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      • KCI등재

        점안용 웅담.우황.사향 약침액의 안점막자극실험

        서형식,Seo, Hyeong-Sik 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.3

        Objective: The aim of this study is to investigate the safety of Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods: The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration(2005. 10. 21, KFDA 2005-60). After Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution was applied eyewash in the left eye of the rabbits, the author observed eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Results: 1. After Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution was applied eyewash in the left eye of the rabbits, there wasn t physical problem at 9 rabbits. 2. After Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution was applied eyewash in the left eye of the rabbits, there wasn't eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Conclusions: In this study, it was observed that Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution didn't induced eye irritation in rabbits.

      • KCI등재후보

        황련의 증량에 따른 전탕액이 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구

        서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.1

        Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to 50${\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples($20{\mu}l$, $30{\mu}l$, $40{\mu}l$, $50{\mu}l$) in 100% density, but anti-bacterial potency of 80g, $50{\mu}l$ Coptidis rhizoma extract decreased compared with 40g, $50{\mu}l$. 3. MIC on Staphylococcus epidermidis in 40g Coptidis rhizoma extract was showed in $50{\mu}l$, $40{\mu}l$ of 10% density, 80g Coptidis rhizoma extract was showed in $50{\mu}l$ of 10% density, 160g Coptidis rhizoma extract was showed in $50{\mu}l$, $40{\mu}l$, $30{\mu}l$ of 10% density. Conclusions : Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, anti-bacterial potency compare with quantity and density must be studied.

      • KCI등재

        개용환(改容丸)의 항염 및 항산화 효과에 대한 실험적 연구

        서형식,Seo, Hyeong-Sik 대한약침학회 2009 Journal of pharmacopuncture Vol.12 No.3

        Objectives : This study was performed to investigate the anti-inflammatory and anti-oxidantic effects of GaeYongHwan(GYH) extract which has been used for patients with acnes. Methods : Anti-inflammatory and anti-oxidantic effects of GYH extract were tested in terms of inhibitory ability of Nitric oxide(NO) production, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity and anti-bacterial effects against Propionibacterium acnes(P. acnes). Results : 1. All GYH treated groups did not show cytotoxicity. 2. Treatment with $100{\mu}g/m{\ell}$ of GYH extract lowered production levels of NO significantly compared to non-treated control or normal. 3. All of GYH treated groups did not show DPPH free radical scavenging activities. 4. All of GYH treated groups did not show anti-baterial action against P. acnes. Conclusions : These results imply that GYH extract has anti-inflammatory effect to treat acnes.

      • KCI등재

        소풍도적탕(消風導赤湯)과 사물소풍산(四物消風散)의 항염 및 항산화 효과에 대한 실험적 연구

        서형식,Seo, Hyung-Sik 대한약침학회 2010 Journal of pharmacopuncture Vol.13 No.1

        Objective: This experimental study was performed to investigate the anti-inflammation and anti-oxidant effects of Sopungdojeok-tang(SPDJT) and Samulsopung-san(SMSPS) which were used to treat patient with atopic dermatitis Methods: Anti-inflammation and anti-oxidant effects of SPDJT and SMSPS were measured by the inhibitory ability of Nitric oxide(NO) production and the scavenging for 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical. Results: 1. SPDJT and SMSPS were not showed cell toxicity. 2. In inhibitory effects against NO production, all groups of SPDJT were showed anti-inflammation, but all groups of SMSPS were not showed anti-inflammation. There were statistical significances between $20{\mu}g/m\ell$ and 4, $10{\mu}g/m\ell$ in SPDJT groups. 3. In DPPH radical scavenging activity, all groups of SPDJT and all groups of SMSPS were showed anti-oxidant effects. There were statistical significances between $20{\mu}g/m\ell$ SPDJT group and 4, $10{\mu}g/m\ell$ SMSPS groups. Conclusion: These results suggest that the SPDJT groups are better than the SMSPS groups in antiinflammation and anti-oxidant effects.

      • SCOPUSKCI등재

        황련의 증량에 따른 전탕액이 Staphylococcus aureus에 미치는 항균효과에 대한 실험적 연구

        서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.3

        Objective This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus aureus that induce keratitis. Methods Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. 3. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples ($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. Anti-bacterial potency of 80g Coptidis rhizoma extract decreased compared with 40g. Anti-bacterial potency of 160g Coptidis rhizoma extract decreased compared with 40g in $20{\mu}l,\;30{\mu}l$. Conclusions Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, antibacterial potency compare with quantity and density must be studied.

      • KCI등재후보

        황련(黃連) 전탕액(煎湯液)이 Staphylococcus species(S.aureus, S.epidermidis)의 배양일에 따른 항균효과의 지속성에 관한 연구

        서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.3

        Objectives This experimental study was performed to investigate the continuous anti-bacterial potency of Coptidis rhizoma extract on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ diluted Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$). Results 1. Anti-bacterial potency of Coptidis rhizoma extract on S. aureus was appeared in 100%, 10% and was the same as anti-bacterial potency of 2 days and 6 days. Anti-bacterial potency with change of volume(100%) was increased in propotion to increase volume on all samples. Anti-bacterial potency with change of volume(10%) was increased in propotion to increase volume on all samples except $20{\mu}l$. Anti-bacterial potency of Coptidis rhizoma extract on S. aureus was appeared continuous. 2. Anti-bacterial potency of Coptidis rhizoma extract on S. epidermidis was appeared in 100%, 10% and was the same as anti-bacterial potency of 2 days and 6 days. Anti-bacterial potency with change of volume(100%) was increased in propotion to increase volume on all samples. Anti-bacterial potency with change of volume(10%) was appeared in $50{\mu}l$. Anti-bacterial potency of Coptidis rhizoma extract on S. epidermidis was appeared continuous. Conclusions Anti-bacterial potency of Coptidis rhizoma extract on cultivation of S. aureus & S. epidermidis was showed continuous.

      • SCOPUSKCI등재

        진피산(秦皮散)이 Staphylococcus species(S. aureus, S. epidermis)의 배양일에 따른 항균과의 지속성에 관한 연구

        서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.2

        Objectives : This experimental study was performed to investigate the continuous anti-bacterial potency of Jinpi-san on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50${\mu}$l diluted Jinpi-san(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(20${\mu}$l, 30${\mu}$l, 40${\mu}$l, 50${\mu}$l). Results : 1. Anti-bacterial potency of Jinpi-san on S. aureus was appeared in 100% and increased on 6 days. Anti-bacterial potency with change of volume was increased in propotion to increase volume. Anti-bacterial potency of Jinpi-san on S. aureus was appeared continuous. 2. Anti-bacterial potency of Jinpi-san on S. epidermidis was appeared in 100%, 10%, 1% on 2 days and in 100%, 10% on 6 days. In 100% Jinpi-san, Anti-bacterial potency of 6 days was increased, in 10%, Anti-bacterial potency of 2 days was increased, in 1%, Anti-bacterial potency of 6 days was disappear. Anti-bacterial potency with change of volume was increased in propotion to increase volume except for 20${\mu}$l of 6days and increased on 6 days, but bacteria was increased. Anti-bacterial potency of Jinpi-san on S. epidermidis wasn't appeared continuous. Conclusions : Anti-bacterial potency of Jinpi-san on cultivation of S. aureus was showed continuous, but on cultivation of S. epidermidis was not showed continuous.

      • KCI등재후보

        황련의 증량에 따른 전탕액이 Pseudomonas aeruginosa에 미치는 항균효과에 대한 실험적 연구

        서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.1

        Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Pseudomonas aeruginosa that induce keratitis. Methods : MIC was measured by dropping to 50 ${\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 120g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : MIC on Pseudomonas aeruginosa in Coptidis rhizoma extract compared with quantity(40g, 80g, 120g, 160g) was not showed all samples(100%, 10%, 1%, 0.1%). Conclusions : Coptidis rhizoma extract compared with quantity was not showed anti-bacterial potency on Pseudomonas aeruginosa. The results differ that Coptidis rhizoma extract was showed anti-bacterial potency in 1999 year. Anti-bacterial Potency of Coptidis rhizoma extract compared with quantity on Pseudomonas aeruginosa must be studied.

      • SCOPUSKCI등재

        결명자(決明子), 청상자(靑?子), 밀몽화(密蒙花) 전탕액(前湯液)의 항균성에 대한 실험적 연구

        서형식,Seo, Hyung-Sik 대한약침학회 2010 Journal of pharmacopuncture Vol.13 No.2

        Objectives : This experimental study was performed to investigate antibiosis of decoctions made by Cassiae Semen(CaS), Celosiae Semen(CeS) and Buddlejae Flos(BF). Methods : Decoctions made by CaS. CeS and BF were prepared. After administering decoctions made by CaS. CeS and BF on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Condida albicans) which cause Keratitis, the size of inhibition zone and MIC(Minimum Inhibition Concentration) were measured Results : The inhibition zone on bacterial species didn't appear, after administering decoctions made by CaS, CeS and BF. Conclusions : This experimental study is showed that decoctions made by CaS, CeS and BF don't have antibiosis on bacterial species which cause Keratitis.

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