siRNA Interference with a Proliferation-Inducing Ligand Gene in the Sgr-7901 Gastric Carcinoma Cell Line

Ni, Shao-Zhong,Cao, Hong-Yong,Chen, Zheng,Zhu, Yi,Xu, Ze-Kuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: The present study aimed to investigate the influence of siRNA interference with a proliferation-inducing ligand (APRIL) gene on gastric carcinoma sgr-7901 cell apoptosis. Correlations between APRIL silencing and tyrosine kinase (trka) expression were also explored. Methods: Two APRIL-silencing siRNA vectors were constructed, and transfected into human gastric carcinoma sgr-7901 cells, expression before and after transfection being detected using RT-PCR and western blot analyses. The expression of 15 trka genes was detected using RT-PCR and apoptotic rates of sgr-7901 were assessed by flow cytometry. Results: The expression levels of receptor trka genes were significantly decreased, and the apoptotic rate of sgr-7901 was significantly increased after transfection (P < 0.05). Conclusion: APRIL gene silencing can increase the apoptotic rate of gastric carcinoma cells, and inhibit the expression of receptor trka genes. There is a correlation between the signaling pathways of APRIL and trka.

Hydrophobic modification of silica/exfoliated graphite nanoplatelets aerogel and its application as supporting material for form-stable phase change materials

Ni Tan,Yang Feng,Ping Hu,QiLin Ding,Chuan-Huang Lin,Yu-Hao Ning,Hao-Nan Zhou,Linping Yu,Zhong Cao,Ju-Lan Zeng 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.99 No.-

Hydrophobic modified silica/exfoliated graphite nanoplatelets aerogel (M-SiO2/xGnP) were successfullyprepared via surface modification of silica/xGnP alcogel and followed by ambient pressure drying. Afterwards, form-stable PCMs in which capric–palmitic acids eutectic (CA–PA) was confined in theprepared aerogels were obtained by vacuum infiltration. Characterization of the prepared form-stablePCMs revealed that both the hydrophobic modification and the doping of xGnP could effectively improvethe loading of CA–PA in the aerogel. The unmodified silica aerogel could not adsorb CA–PA, while theloading of CA–PA in the surface modified pure silica aerogel supported form-stable PCM and theunmodified silica/xGnP aerogel supported form-stable PCM were 24.2 wt% and 44.4 wt%, respectively. Besides, the hydrophobic modification and the doping of xGnP showed significant synergistic effect. Theloading of CA–PA in the M-SiO2/xGnP supported form-stable PCM (FPCM/xGnP-20-48) could attain78.9 wt% when the M-SiO2/xGnP was obtained by modifying the alcogel with 20 vol% trimethylchlorosilane for 48 h. The FPCM/xGnP-20-48 not only had high latent heat and good thermal reliability,but also exhibited significantly improved thermal conductivity and alleviated supercooling due to theeffective thermal conductive network formed by xGnP and the promoted heterogeneous nucleation ofCA–PA at interfaces with aerogel.

Identification of a Novel Human Zinc Finger Gene, ZNF438, with Transcription Inhibition Activity

Zhong, Zhaomin,Wan, Bo,Qiu, Yun,Ni, Jun,Tang, Wenwen,Chen, Xinya,Yang, Yun,Shen, Suqin,Wang, Ying,Bai, Meirong,Lang, Qingyu,Yu, Long Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.4

There were many different families of zinc finger proteins that contained multiple cysteine and/or histidine residues and used zinc to stabilize their folds. The classical C2H2 zinc finger proteins were the founding members of this superfamily and were among the most abundant proteins in eukaryotic genomes. C2H2 proteins typically contained several C2H2 fingers that made tandem contacts along the DNA. Here we reported a novel C2H2 type zinc finger gene, ZNF438, which encoded 828 amino acids that formed five zinc finger domains. Bioinformatics analysis revealed that the ZNF438 was mapped to human chromosome 10p11.2 and shared 62% identity with rat and mouse homologues. RT-PCR analysis indicated that it was ubiquitously expressed in 18 human adult tissues. With immunofluorescence assay, it was shown that the exogenous Flag-tagged ZNF438 was located in nucleus of COS-7 cells. To further explore the function of ZNF438, we examined the transcriptional activity of ZNF438 protein by transfecting recombinant pM-ZNF438 into mammalian cells. The subsequent analysis based on the duel luciferase assay system showed that ZNF438 was a transcriptional repressor.

Numerical simulation of the sheet/cloud cavitation around a two-dimensional hydrofoil using a modified URANS approach

Zhong Li,Dawei Zheng,Feng Hong,Dan Ni 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.1

Cavitating flow over a two-dimensional Clark-Y hydrofoil is numerically investigated via a Modified density correction method (MDCM) for turbulence closure to improve the capability of two-phase flow simulation for the k-ε RNG turbulence model. A transport equation model for the local volume fraction of vapor is solved, and a modified version of the Kunz model is used for the condensation and evaporation processes. Simulations have been conducted for various cavitation numbers ranging from non-cavitating to cloud cavitation. We compared the time-averaged lift and drag coefficients, cavitation dynamics, and time-averaged velocity profiles with the available experimental data for the MDCM and Density correction method (DCM). The comparisons between numerical and experimental results show that the MDCM and DCM are capable of capturing the special trends of the lift coefficient at the inception cavitation stage and the drag coefficient at the cloud cavitation stage. The MDCM is more robust and physical than the DCM in predicting the wake flow downstream from the trailing edge. The predicted attached cavities of both models almost show the same trend near the leading edge of the hydrofoil. However, the MDCM predicts more shedding cavity than the DCM in the wake region.

The Inhibition Effect of Triptolide on Human Endometrial Carcinoma Cell Line HEC-1B: a in vitro and in vivo Studies

Ni, Jing,Wu, Qiang,Sun, Zhi-Hua,Zhong, Jian,Cai, Yu,Huang, Xin-En Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

Background: To investigate the inhibitory effect and the underlying mechanism of triptolide on cultured human endometrial carcinoma HEC-1B cells and corresponding xenograft. Materials and Methods: For in vitro studies, the inhibition effect of proliferation on HEC-1B cell by triptolide was determined by MTT assay; cell cycle and apoptosis of the triptolide-treated and untreated cells were detected by flow cytometry. For in vivo studies, a xenograft tumor model of human endometrial carcinoma was established using HEC-1B cells, then the tumor-bearing mice were treated with high, medium, and low-dose ($8{\mu}g$, $4{\mu}g$ and $2{\mu}g/day$) triptolide or cisplatin at $40{\mu}g/day$ or normal saline as control. The mice were treated for 10-15 days, during which body weight of the mice and volume of the xenograft were weighted. Then expression of Bcl-2 and vascular endothelial growth factor (VEGF) was analyzed by SABC immunohistochemistry. Results: Cell growth was significantly inhibited by triptolide as observed by an inverted phase contrast microscope; the results of MTT assay indicated that triptolide inhibits HEC-1B cell proliferation in a dose and time-dependent manner; flow cytometry showed that low concentration (5 ng/ml) of triptolide induces cell cycle arrest of HEC-1B cells mainly at S phase, while higher concentration (40 or 80 ng/ml) induced cell cycle arrest of HEC-1B cells mainly at G2/M phase, and apoptosis of the cells was also induced. High-dose triptolide showed a similar tumor-inhibitory effect as cisplatin (-50%); high-dose triptolide significantly inhibited Bcl-2 and VEGF expression in the xenograft model compared to normal saline control (P<0.05). Conclusions: triptolide inhibits HEC-1B cell growth both in vitro and in mouse xenograft model. Cell cycle of the tumor cells was arrested at S and G2/M phase, and the mechanism may involve induction of tumor cell apoptosis and inhibition of tumor angiogenesis.

Ginsenoside Rg1 ameliorates Alzheimer's disease pathology via restoring mitophagy

Ni Wang,Junyan Yang,Ruijun Chen,Yunyun Liu,Shunjie Liu,Yining Pan,Qingfeng Lei,Yuzhou Wang,Lu He,Youqiang Song,Zhong Li The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.3

Background: Alzheimer's disease (AD) is a common form of dementia, and impaired mitophagy is a hallmark of AD. Mitophagy is mitochondrial-specific autophagy. Ginsenosides from Ginseng involve in autophagy in cancer. Ginsenoside Rg1 (Rg1 hereafter), a single compound of Ginseng, has neuroprotective effects on AD. However, few studies have reported whether Rg1 can ameliorate AD pathology by regulating mitophagy. Methods: Human SH-SY5Y cell and a 5XFAD mouse model were used to investigate the effects of Rg1. Rg1 (1µM) was added to β-amyloid oligomer (AβO)-induced or APPswe-overexpressed cell models for 24 hours. 5XFAD mouse models were intraperitoneally injected with Rg1 (10 mg/kg/d) for 30 days. Expression levels of mitophagy-related markers were analyzed by western blot and immunofluorescent staining. Cognitive function was assessed by Morris water maze. Mitophagic events were observed using transmission electron microscopy, western blot, and immunofluorescent staining from mouse hippocampus. The activation of the PINK1/Parkin pathway was examined using an immunoprecipitation assay. Results: Rg1 could restore mitophagy and ameliorate memory deficits in the AD cellular and/or mouse model through the PINK1-Parkin pathway. Moreover, Rg1 might induce microglial phagocytosis to reduce β-amyloid (Aβ) deposits in the hippocampus of AD mice. Conclusion: Our studies demonstrate the neuroprotective mechanism of ginsenoside Rg1 in AD models. Rg1 induces PINK-Parkin mediated mitophagy and ameliorates memory deficits in 5XFAD mouse models.

Numerical analysis and experimental investigation on impeller-volute match characteristics

Yongyan Ni,Xiao Wang,Zhong-yong Pan 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.11

Impeller-volute match is an essential issue of pump dynamic characteristics and it is very important for the understanding of the flow features in the pumps and the vibration symptom of the device. Advanced performance diagnosis and smart operation and maintenance are profoundly dependent upon the perceiving of the rotor-stator interaction. In order to describe the interaction more comprehensively, the related flows were described by both theoretical approach and numerical simulations. Both velocity distribution and area-ratio aspect were theoretically indicated and a critical condition of non-shock criterion was proposed. Numerical efforts were therefore carried out and unveiled the flow regime as well as the pressure variations. The frequency properties of the pressure variations indicated that both the shaft rotation frequency and the blade passing frequency (BPF) dominated the contents, which implicitly demonstrated the accurate simulation work. Vibration content was lastly tested to validate the numerical results and the rotor-stator interaction effects. No significant shaft rotation contents appeared in the tested vibration signals due to the precise shaft support. Other than the blade passing frequency, both sub-synchronous and super-synchronous contents of BPFs appear under nearly all conditions. It is noted that the experimental means used here are able to get sufficient information of the pump operation.

A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C

Yao-zhong Ding,Jian-hua Zhou,Li-na Ma,Yan-ni Qi,Gang Wei,Jie Zhang,Yong-guang Zhang 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.3

A reverse transcription loop-mediated isothermalamplification (RT-LAMP) assay was developed to rapidlydetect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples,sensitivity of the FMDV C RT-LAMP was found to be 10times higher than that of conventional reverse transcription-PCR (RT-PCR). No cross-reactivity with A, Asia 1, or OFMDV or swine vesicular disease virus (SVDV) indicatedthat FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.

Numerical investigation on radial impeller induced vortex rope in draft tube under partial load conditions

Yongyan Ni,Renqing Zhu,Xiao Zhang,Zhong-yong Pan 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.1

The vortex rope phenomena appearing in a draft tube under partial load operations were investigated. The efforts were carried out by using a homogeneous equilibrium vapor-liquid two-phase model and a blending SST turbulence model, and the former takes account of the weak compressibility of mixed media. The vortex rope phenomena were induced by the radial impeller and three kinds of flow regimes were illustrated. Vortex rope was visible at some conditions and it displayed various patterns, while no obvious vortex rope was found under some conditions. It was found that the plant cavitation number determines that whether the vortex rope was visible or invisible. An interesting profile was that a secondary vortex filament ring entwining the vortex rope appeared clearly near the design condition. Both flow structures and pressure characteristics were studied to depict the vortex mechanism and the characteristics of the η-σ curves. Further analysis found that the shedding frequency of the vortex ring is similar with that of the precession movement.

Identification of a Novel Human Zinc Finger Gene, ZNF438, with Transcription Inhibition Activity

( Zhao Min Zhong ),( Bo Wan ),( Yun Qiu ),( Jun Ni ),( Wen Wen Tang ),( Xin Ya Chen ),( Yun Yang ),( Su Qin Shen ),( Ying Wang ),( Mei Rong Bai ),( Qing Yu Lang ),( Long Yu ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.4