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Effects of regeneration conditions on sulfated CuSSZ-13 catalyst for NH3-SCR
Meiqing Shen,Zhixin Wang,Xinhua Li,Jiaming Wang,Jianqiang Wang,Chen Wang,Jun Wang 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.8
To understand the role of regeneration conditions on sulfur-poisoned Cu/SSZ-13 catalysts for NH3-SCR, the physicochemical characterizations and NOx conversions were investigated. The sulfur-poisoned Cu/SSZ-13 catalysts were treated at different conditions as a function of temperature and duration. TGA results revealed that regeneration at 500 oC only removed parts of sulfur spices and at 700 oC can completely remove all sulfur spices. The physical structural characterizations results illustrate that regeneration below 700 oC has no significant impact on CHA structure for Cu/SSZ-13 catalysts, while dealumination occurs on poisoned Cu/SSZ-13 when regeneration temperature is higher than 700 oC. EPR and H2-TPR results show that the sulfate decomposition and Cu migration reactions involved during regeneration and, as a result, the content of Cu2+ gradually increased as the extent of regeneration increased. The kinetics tests support that NOx conversion recovery is related to the content of Cu2+ increase during regeneration. Our study reveals that the optimum regeneration temperature is 700 oC, because severe dealumination at 750 oC inhibited Cu2+ amount increase.
Wang Wei,Zhang Zhixin,Zhang Chuanbao,Zhao Haijian,Yuan Shuai,Liu Jiali,Dong Na,Wang Zhiguo,Kang Fengfeng 대한진단검사의학회 2024 Annals of Laboratory Medicine Vol.44 No.3
Background: Clinical chemistry tests are most widely used in clinical laboratories, and diverse measurement systems for these analyses are available in China. We evaluated the imprecision of clinical chemistry measurement systems based on internal QC (IQC) data. Methods: IQC data for 27 general chemistry analytes were collected in February each year from 2013 to 2022. Four performance specifications were used to calculate pass rates for CVs of IQC data in 2022. Boxplots were drawn to analyze trends of CVs, and differences in CVs among different groups were assessed using the Mann–Whitney U-test or Kruskal–Wallis test. Results: The number of participating laboratories increased significantly from 1,777 in 2013 to 5,425 in 2022. CVs significantly decreased for all 27 analytes, except creatine kinase and lipase. Triglycerides, total bilirubin, direct bilirubin, iron, and γ-glutamyl transferase achieved pass rates >80% for all goals. Nine analytes with pass rates <80% based on 1/3 allowable total error were further analyzed; the results indicated that closed systems exhibited lower CVs than open systems for all analytes, except total protein. For all nine analytes, differences were significant between tertiary hospitals and non-tertiary hospitals and between accredited and non-accredited laboratories. Conclusions: The CVs of IQC data for clinical chemistry have seen a continuous overall improvement in China. However, there is ample room for imprecision improvement for several analytes, with stricter performance specifications.
Jina Wang, Kaidong Xu,Zhixin Li,Yilong Yang,Qingxiao Li,Yun Bao,Huan Yang,Lingling Ding,Ruixin Zhang,Yuanyuan Wang,Lan Yao 한양대학교 세라믹연구소 2022 Journal of Ceramic Processing Research Vol.23 No.1
In order to effectively reuse red mud and coal gangue, foam glass was successfully prepared by sintering. The foaming of foamglass is ensured by adding foaming agent, foam stabilizer and fluxing agent to the raw materials including red mud, coalgangue and waste glass. Furthermore, the defects in the foam glass are also reduced. The obtained powder was dried at 105ºC and characterized by XRF, DSC-TG analysis, etc. According to cellular structure, apparent density and water absorption,the optimal type and ratio of foaming agent, foaming stabilizer and fluxing agent were revealed. The results indicate that theoptimized formula of foaming agent, foaming stabilizer and fluxing agent is 3% MnO2-2% Na3PO4-2% borax. The foam glassprepared under optimized conditions exhibits uniform porous distribution and suitable pore size with an apparent density of0.575 g/cm3 and water absorption of 4.61%. The research in this work could establish the technical foundation of red mudbasedporous material for industrial production.
Guo, Zhixin,Wang, Yanfeng,Feng, Xue,Bao, Chaogetu,He, Qiburi,Bao, Lili,Hao, Huifang,Wang, Zhigang Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.11
Mammalian target of rapamycin complex 1 (mTORC1) is a central regulator of cell growth and metabolism and is sufficient to induce specific metabolic processes, including de novo lipid biosynthesis. Elongation of very-long-chain fatty acids 1 (ELOVL1) is a ubiquitously expressed gene and the product of which was thought to be associated with elongation of carbon (C) chain in fatty acids. In the present study, we examined the effects of rapamycin, a specific inhibitor of mTORC1, on ELOVL1 expression and docosahexaenoic acid (DHA, C22:6 n-3) synthesis in bovine mammary epithelial cells (BMECs). We found that rapamycin decreased the relative abundance of ELOVL1 mRNA, ELOVL1 expression and the level of DHA in a time-dependent manner. These data indicate that ELOVL1 expression and DHA synthesis are regulated by mTORC1 in BMECs.
Yanfeng Wang,Xudong Guo,Zhigang Wang,Zhixin Guo,Mingtao Liu,Keyu Zhao,Xue Feng,Xu Zheng,Xiaojing Wang,Huifang Hao 한국통합생물학회 2017 Animal cells and systems Vol.21 No.5
PSMB1 (proteasome subunit beta type 1) is a core component of the 20S proteasome, and based on its structure, it might have a crucial function in the transcription of certain genes. Rheb (Ras homolog enriched in brain) is a Ras-like small GTPase that acts as an upstream positive regulator of the mechanistic target of rapamycin complex 1 (mTORC1) pathway. We cloned and characterized PSMB1 (KY310590.1) to determine its function in cell cycle progression and proliferation of goat fetal fibroblasts (GFbs). Further, by yeast two-hybrid screen and coimmunoprecipitation, we conformed that PSMB1 interacts directly with Rheb. An siRNA was designed and expressed targeting PSMB1 mRNA in GFbs and inducing cell cycle arrest. Rheb overexpression in GFbs significantly increased the number of S phase cells and growth efficiency compared with control cells. These data indicate that PSMB1 and Rheb have important functions in the cell cycle and proliferation of GFbs, indicating that their interaction governs many processes in GFbs.
Hui Ji,Hui Wang,Qiumei Ji,Winbo Ji,Xiaolin Luo,Jiabo Wang,Zhixin Chai,Jinwei Xin,Xin Cai,Zhijuan Wu,Jikun Wang,Jincheng Zhong 한국유전학회 2020 Genes & Genomics Vol.42 No.11
Background miRNAs play an important role in regulating normal animal development. Muscle tissue and fat metabolismare important for maintaining energy balance in animals. Yak has important agricultural and economic importance as itprovides milk, meat, and hair. It is used for transportation as well. However, the miRNA expression profiles of their muscleand adipose tissue are currently unknown. Objective To explore the regulatory roles of miRNAs in the skeletal muscle and adipose tissues of yak. Methods A total of 12 small RNA libraries were constructed from the skeletal muscle and adipose samples from yak aged 0.5,2.5, 4.5, and 7.5 years. High-throughput sequencing and bioinformatics analysis were used to determine the dynamic expressionprofile of miRNA, and a miRNA regulatory network related to muscle and adipose tissue development was established. Results miR-1-3p and miR-143-3p showed the highest expression during yak skeletal muscle and fat development, respectively. The MAPK and Ras signaling pathways were the pivotal pathways. miR-181-5p, miR-542-3p, and miR-424-5p mayhave key roles in skeletal muscle development, and CREBRF, GRB10, CDK1, RFX3, and EPC2 were the core target genes. While miR-127-5p, miR-379-3p, and miR-494-3p may play important regulatory roles in adipose deposition, and ETV1,XPO7, and C5AR2 were the core target genes. Conclusion This study provides valuable resources for further study of the molecular mechanisms underlying yak skeletalmuscle and adipose tissue development, and also a basis for studying the interactions between genes and miRNAs.
Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
Bao, Wenlei,Yin, Jianxin,Liang, Yan,Guo, Zhixin,Wang, Yanfeng,Liu, Dongjun,Wang, Xiao,Wang, Zhigang Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.9
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant $6{\times}his-gVEGF164$ protein was induced by 0.5 mM isopropyl thio-${\beta}$-D-galactoside at $32^{\circ}C$. Recombinant goat VEGF164 (rgVEGF164) was purified and identified by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.
Expression of fox-related genes in the skin follicles of Inner Mongolia cashmere goat
Wenjing Han,Xiaoyan Li,Lele Wang,Honghao Wang,Kun Yang,Zhixin Wang,Ruijun Wang,Rui Su,Zhihong Liu,Yanhong Zhao,Yanjun Zhang,Jinquan Li 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3
Objective: This study investigated the expression of genes in cashmere goats at different periods of their fetal development. Methods: Bioinformatics analysis was used to evaluate data obtained by transcriptome sequencing of fetus skin samples collected from Inner Mongolia cashmere goats on days 45, 55, and 65 of fetal age. Results: We found that FoxN1, FoxE1, and FoxI3 genes of the Fox gene family were probably involved in the growth and development of the follicle and the formation of hair, which is consistent with previous findings. Real-time quantitative polymerase chain reaction detecting system and Western blot analysis were employed to study the relative differentially expressed genes FoxN1, FoxE1, and FoxI3 in the body skin of cashmere goat fetuses and adult individuals. Conclusion: This study provided new fundamental information for further investigation of the genes related to follicle development and exploration of their roles in hair follicle initiation, growth, and development.
Inositol Requiring Enzyme (IRE), a multiplayer in sensing endoplasmic reticulum stress
Zhixin Zhou,Qian Wang,Marek Michalak 한국통합생물학회 2021 Animal cells and systems Vol.25 No.6
The endoplasmic reticulum (ER) can sense a wide variety of external and internal perturbations and responds by mounting stress coping responses, such as the unfolded protein response (UPR). The UPR is composed of three stress sensors, namely IRE1α, PERK, and ATF6 that are activated to reestablish ER homeostasis. IRE1α represents the most ancient branch of the UPR affecting many cellular processes in plant and animal cells. IRE1α is a type I transmembrane protein with kinase/nuclease activities in response to ER stress. Both the ER luminal and cytosolic IRE1α interactomes have been identified revealing a multifunctional role of the ER stress sensor. IRE1α is also associated with organellar membrane contacts to promote rapid communication between intracellular organelles under stress conditions.
Density-based Outlier Detection in Multi-dimensional Datasets
Xite Wang,Zhixin Cao,Rongjuan Zhan,Mei Bai,Qian Ma,Guanyu Li 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.12
Density-based outlier detection is one of the hot issues in data mining. A point is determined as outlier on basis of the density of points near them. The existing density-based detection algorithms have high time complexity, in order to reduce the time complexity, a new outlier detection algorithm DODMD (Density-based Outlier Detection in Multidimensional Datasets) is proposed. Firstly, on the basis of ZH-tree, the concept of micro-cluster is introduced. Each leaf node is regarded as a micro-cluster, and the micro-cluster is calculated to achieve the purpose of batch filtering. In order to obtain n sets of approximate outliers quickly, a greedy method is used to calculate the boundary of LOF and mark the minimum value as LOF i . Secondly, the outliers can filtered out by LOF i , the real outliers are calculated, and then the result set is updated to make the boundary closer. Finally, the accuracy and efficiency of DODMD algorithm are verified on real dataset and synthetic dataset respectively.