A Method for Determining Optimum Particle Size Distribution Based on Fractal Theory

( Zhihong Zhang ),( Fan Yang ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

The particle-size distribution is one of fundamental soil physical properties. In this study the fractal theory was applied to describe the distribution of partial size, and the relationship among particle mass, gradation curve of particle size and fractal dimension is determined. According to the principle that the mechanical properties is optimum when the fractal dimension of filler is unique, the method for determining optimum particle size distribution based on fractal theory has been proposed, based on the one-dimensional fractal characteristics of filling integral. The advantage of the presented method is not including unknown parameters, which is available for soil and rock. The indoors consolidation tests in low and high pressures and numerical simulations with the software Power Factor Correction are conducted to testify the validation of presented method. The results show that the proposed method for determining optimum particle size based on fractal theory is prior to the existing methods. The smaller deformations are observed by the proposed method than others. This investigation is suitable for artificial filling to reach the densest state in the practical engineering.

Lnc Tmem235 promotes repair of early steroid-induced osteonecrosis of the femoral head by inhibiting hypoxia-induced apoptosis of BMSCs

Zhang Fei,Peng Wuxun,Wang Tao,Zhang Jian,Dong Wentao,Wang Chuan,Xie Zhihong,Luo Hong,Liu Gang 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Bone marrow mesenchymal stem cells (BMSCs) have been used in the treatment of early steroid-induced osteonecrosis of the femoral head (SONFH). However, the hypoxic microenvironment in the osteonecrotic area leads to hypoxia-induced apoptosis of transplanted BMSCs, which limits their efficacy. Therefore, approaches that inhibit hypoxia-induced apoptosis of BMSCs are promising for augmenting the efficacy of BMSC transplantation. Our present study found that under hypoxia, the expression of the long noncoding RNA (Lnc) transmembrane protein 235 (Tmem235) was downregulated, the expression of Bcl-2-associated X protein was upregulated, the expression of B-cell lymphoma-2 protein was downregulated, and the apoptotic rate of BMSCs was over 70%. However, overexpression of Lnc Tmem235 reversed hypoxia-induced apoptosis of BMSCs and promoted their survival. These results demonstrated that Lnc Tmem235 effectively inhibited hypoxia-induced apoptosis of BMSCs. Mechanistically, we found that Lnc Tmem235 exhibited competitive binding to miR-34a-3p compared with BIRC5 mRNA, which is an inhibitor of apoptosis; this competitive binding relieved the silencing effect of miR-34a-3p on BIRC5 mRNA to ultimately inhibit hypoxia-induced apoptosis of BMSCs by promoting the expression of BIRC5. Furthermore, we cocultured BMSCs overexpressing Lnc Tmem235 with xenogeneic antigen-extracted cancellous bone to construct tissue-engineered bone to repair a model of early SONFH in vivo. The results showed that overexpression of Lnc Tmem235 effectively reduced apoptosis of BMSCs in the hypoxic microenvironment of osteonecrosis and improved the effect of BMSC transplantation. Taken together, our findings show that Lnc Tmem235 inhibited hypoxia-induced apoptosis of BMSCs by regulating the miR-34a-3p/BIRC5 axis, thus improving the transplantation efficacy of BMSCs for treating early SONFH.

Research on dynamic load estimation method of crawler travel system

Zhihong Zhang,Hong Zhang,You Chen,Honghong Yan 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.2

This paper proposed a novel approach for dynamic load estimation. The similarity criteria between the model vehicle and the prototype vehicle were established based on the structural, operating, and contact parameters of the crawler travel system. The rigid and flexible coupling simulation models of the model vehicle and the prototype vehicle were established, respectively. The accuracy of the simulation model of the model vehicle was verified by the vibration acceleration and stress load obtained by the experiment of the model vehicle. The accuracy of the simulation model of the prototype vehicle (SMOP) was verified by theoretical calculation of the travel resistance torque. Results indicated that the relative error between the dynamic load estimated based on the similarity theory and the dynamic load obtained by the SMOP was 14.6 %. This research helps to reduce the test cost and provides a theoretical basis for the preliminary research on construction machinery.

Research on Covert Communication Technology Based on Matrix Decomposition of Digital Currency Transaction Amount

Lejun Zhang,Bo Zhang,Ran Guo,Zhujun Wang,Guopeng Wang,Jing Qiu,Shen Su,Yuan Liu,Guangxia Xu,Zhihong Tian,Sergey Gataullin 한국인터넷정보학회 2024 KSII Transactions on Internet and Information Syst Vol.18 No.4

With the development of covert communication technologies, the number of covert communication technologies using blockchain as a carrier is increasing. However, using the transaction amount of digital currency as a carrier for covert communication has problems such as low embedding rate, large consumption of transaction amount, and easy detection. In this paper, firstly, by experimentally analyzing the distribution of bitcoin transaction amounts, we determine the most suitable range of amounts for matrix decomposition. Secondly, we design a novel matrix decomposition method that can successfully decompose a large amount matrix into two small amount matrices and utilize the elements in the small amount matrices for covert communication. Finally, we analyze the feasibility of the novel matrix decomposition method in this scheme in detail from four aspects, and verify it by experimental comparison, which proves that our scheme not only improves the embedding rate and reduces the consumption of transaction amount, but also has a certain degree of resistance to detection.

An Improved Genetic Algorithm Based on Combination Theory and Fuzzy Reasoning Technique and its Applications

Zhang Liangjie,Mao Zhihong,Li Yanda 대한전자공학회 1994 ICONIP : International Conference On Neural Inform Vol.1 No.3

Research and Application of Video Surveillance for Substation Sequence Control

Zhang, Zhixue,Huo, Zhihong 대한전기학회 2014 The Journal of International Council on Electrical Vol.4 No.3

Video surveillance system has been set up in new-built or reformed smart substations for safe operation, fire alarm and assets safety. In this paper video surveillance system has been developed for operation, especially for programmed operation of smart substation. Therefore, video image information besides conventional signals such as telemetry, remote signal, telecontrol, and remote regulating has been integrated in the novel integrated system to assist safety operation. The system architecture has been divided into three components and introduced in this paper firstly. Communication protocol has been developed for exchange information between integrated SCADA system and video surveillance system.

Identification and Validation of Potential Conserved microRNAs and Their Targets in Peach (Prunus persica)

Zhihong Gao,Xiaoyan Luo,Ting Shi,Bin Cai,Zhen Zhang,Zongming Cheng,Weibing Zhuang 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.3

MicroRNAs are a class of small, endogenous, non-coding RNA molecules that negatively regulate gene expression at the transcriptional or the post-transcriptional level. Although a large number of miRNAs have been identified in many plant species, especially from model plants and crops, they remain largely unknown in peach. In this study, 110 potential miRNAs belonging to 37 families were identified using computational methods. A total of 43 potential targets were found for 21 families based on near-perfect or perfect complementarity between the plant miRNA and the target sequences. A majority of the targets were transcription factors which play important roles in peach development. qRT-PCR analysis of RNA samples prepared from different peach tissues for 25 miRNA families revealed that miRNAs were differentially expressed in different tissues. Furthermore, two target genes were experimentally verified by detection of the miRNA-mediated mRNA cleavage sites in peach using RNA ligase-mediated 5’ rapid amplification of cDNA ends (RLM-RACE). Finally, we studied the expression pattern of the two target genes in three different tissues of peach to further understand the mechanism of the interaction between miRNAs and their target genes.

Rapid Detection of Lily mottle virus and Arabis mosaic virus Infecting Lily (Lilium spp.) Using Reverse Transcription Loop-Mediated Isothermal Amplification

Yubao Zhang,Yajun Wang,Zhongkui Xie,Ruoyu Wang,Zhihong Guo,Yuhui He 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.2

The Lily mottle virus (LMoV) impedes the growth and quality of lily crops in Lanzhou, China. Recently Arabis mosaic virus (ArMV) has been detected in LMoV- infected plants in this region, causing plant stunting as well as severe foliar symptoms, and likely posing a threat to lily production. Consequently, there is a need to develop simple, sensitive, and reliable detection methods for these two viruses to prevent them from spreading. Reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assays have been de- veloped to detect LMoV and ArMV using two primer pairs that match six conserved sequences of LMoV and ArMV coat proteins, respectively. RT-LAMP assay results were visually assessed in reaction tubes using green fluorescence and gel electrophoresis. Our assays successfully detected both LMoV and ArMV in lily plants without the occurrence of viral cross-reactivity from other lily viruses. Optimal conditions for LAMP reactions were 65°C and 60°C for 60 min for LMoV and ArMV, respectively. Detection sensitivity for both RT-LAMP assays was a hundredfold greater than that of our comparative RT-polymerase chain reaction as- says. We have also found this relatively rapid, target specific and sensitive method can also be used for samples collected in the field and may be especially useful in regions with limited or no laboratory facilities.

Rapid and Sensitive Detection of Lettuce Necrotic Yellows Virus and Cucumber Mosaic Virus Infecting Lettuce (Lactuca sativa L.) by Reverse Transcription Loop-Mediated Isothermal Amplification

Yubao Zhang,Zhongkui Xie,John D Fletcher,Yajun Wang,Ruoyu Wang,Zhihong Guo,Yuhui He 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.1

Cucumber mosaic virus (CMV) is damaging to the growth and quality of lettuce crops in Lanzhou, China. Recently, however, for the first time an isolate of lettuce necrotic yellows virus (LNYV) has been detected in lettuce crops in China, and there is concern that this virus may also pose a threat to lettuce production in China. Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed to detect the nucleoprotein (N) and coat protein (CP) genes of LNYV and CMV, respectively. RT-LAMP amplification products were visually assessed in reaction tubes separately using green fluorescence and gel electrophoresis. The assays successfully detected both viruses in infected plants without cross reactivity recorded from either CMV or LNYV or four other related plant viruses. Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg2+ at 65°C for LNYV and 60°C for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. This rapid, specific, and sensitive technique should be more widely applied due to its low cost and minimal equipment requirements.

Titanium Oxide Film : A New Biomaterial For Artificial Heart Valve Prepared by Ion Beam Enhanced Deposition

Liu, Xianghuai,Zhang, Feng,Zheng, Zhihong,Huang, Nan The Korean Vacuum Society 1997 Applied Science and Convergence Technology Vol.6 No.1

Titanium oxide films were prepared by ion beam enhanced deposition where the films were synthesized by deposition titianium atoms and simultaneously bombarding with xenon ion beam at an energy of 40 keV in an $O_2$ environ,ent. Structure and composition of titanium oxide films were investigated by X-ray Doffractopm (XRD) Ritjerfprd Backscattering Spectroscopy (RBS) and X-ray Diffraction(XRD) Rutherford Backscattering Spectroscopy (RBS) and X-ray photoelectron spectroscopy (XPS) The results show that thestructure of the prepared films exhibit a rutile phase structure wit high(200) orientation and the O/Ti ratio of the titanium oxide films was about 2:1 XPS anlysis shows that $Ti^{2+},Ti^{3+}\;and\;Ti^{4+}$ chemical states exist on the titanium oxide films. the blood compatibility of the titanium oxide films was studied by measurements of blood clotting time and platelet adhesion. The results show that the anticoagulation property of titanium oxide films improved significantly and better than that of LTI-carbon which was widely used to fabricate artificial heart valve.