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Targeting treatment of bladder cancer using PTK7 aptamer-gemcitabine conjugate
Xiang Wei,Peng Yongbo,Zeng Hongliang,Yu Chunping,Zhang Qun,Liu Biao,Liu Jiahao,Hu Xing,Wei Wensu,Deng Minhua,Wang Ning,Liu Xuewen,Xie Jianfei,Hou Weibin,Tang Jin,Long Zhi,Wang Long,Liu Jianye 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Gemcitabine (GEM) is one of the first-line chemotherapies for bladder cancer (BC), but the GEMs cannot recognize cancer cells and have a low long-term response rate and high recurrence rate with side effects during the treatment of BC. Targeted transport of GEMs to mediate cytotoxicity to tumor and avoid the systemic side effects remains a challenge in the treatment of BC.Based on a firstly confirmed biomarker in BC-protein tyrosine kinase 7 (PTK7), which is overexpressed on the cell membrane surface in BC cells, a novel targeting system protein tyrosine kinase 7 aptamer-Gemcitabine conjugate (PTK7-GEMs) was designed and synthesized using a specific PTK7 aptamer and GEM through auto-synthesis method to deliver GEM against BC. In addition, the antitumor effects and safety evaluation of PTK7-GEMs was assessed with a series of in vitro and in vivo assays.PTK7-GEMs can specifically bind and enter to BC cells dependent on the expression levels of PTK7 and via the macropinocytosis pathway, which induced cytotoxicity after GEM cleavage from PTK7-GEMs respond to the intracellular phosphatase. Moreover, PTK7-GEMs showed stronger anti-tumor efficacy and excellent biosafety in three types of tumor xenograft mice models.These results demonstrated that PTK7-GEMs is a successful targeted aptamer-drug conjugates strategy (APDCs) to treat BC, which will provide new directions for the precision treatment of BC in the field of biomarker-oriented tumor targeted therapy.
<i>Chd7</i> Is Critical for Early T-Cell Development and Thymus Organogenesis in Zebrafish
Liu, Zhi-Zhi,Wang, Zi-Long,Choi, Tae-Ik,Huang, Wen-Ting,Wang, Han-Tsing,Han, Ying-Ying,Zhu, Lou-Yin,Kim, Hyun-Taek,Choi, Jung-Hwa,Lee, Jin-Soo,Kim, Hyung-Goo,Zhao, Jian,Chen, Yue,Lu, Zhuo,Tian, Xiao-L Elsevier 2018 The American journal of pathology Vol.188 No.4
<P>Coloboma, heart defect, atresia choanae, retarded growth and development, genital hypoplasia, ear anomalies/deafness (CHARGE) syndrome is a congenital disorder affecting multiple organs and mainly caused by mutations in CHD7, a gene encoding a chromatin-remodeling protein. Immunodeficiency and reduced T cells have been noted in CHARGE syndrome. However, the mechanisms underlying T lymphopenia are largely unexplored. Herein, we observed dramatic decrease of T cells in both chd7knockdown and knockout zebrafish embryos. Unexpectedly, hematopoietic stem and progenitor cells and, particularly, lymphoid progenitor cells were increased peripherally in nonthymic areas in chd7-deficient embryos, unlikely to contribute to the T-cell decrease. Further analysis demonstrated that both the organogenesis and homing function of the thymus were seriously impaired. Chd7 might regulate thymus organogenesis through modulating the development of both neural crest cell-derived mesenchyme and pharyngeal endoderm-derived thymic epithelial cells. The expression of faxn1, a central regulator of thymic epithelium, was remarkably down-regulated in the pharyngeal region in chd7-deficient embryos. Moreover, the T-cell reduction in chd7-deficient embryos was partially rescued by overexpressingfoxnl, suggesting that restoring thymic epithelium may be a potential therapeutic strategy for treating immunodeficiency in CHARGE syndrome. Collectively, the results indicated that chd7 was critical for thymic development and T-lymphopenia in CHARGE syndrome may be mainly attributed to the defects of thymic organogenesis. The current finding may benefit the diagnosis and therapy of T lymphopenia and immunodeficiency in CHARGE syndrome.</P>
Performing Side-Effects in Restricted AND/OR Parallel Execution model of Prolog
Liu, Zhi Long,Hu, Shou Ren 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
Logic programming has gained its popularity in symbolic processing. A question that naturally arises is how to execute standard sequential prolog programs with side-effects in parallel. The problems of performing side-effects in AND parallel systems and in OR parallel systems have been considered elsewhere. This paper makes an inquiry into how to control side-effects in an Restricted AND/OR parallel execution model. The synchronization blocks are added in compiling time. The extension of the Warren abstract instruction is given out. Finally, our future work is given out.
Zhi Hui Liu,Wen Jun Xu,Sheng Yu Li,Cheng Zhi Long,Jia Ru Lin 한국전자통신연구원 2016 ETRI Journal Vol.38 No.5
In this paper, cognitive radio and simultaneous wireless information and power transfer (SWIPT) are effectively combined to design a spectrum-efficient and energy-efficient transmission paradigm. Specifically, a novel SWIPT-based primary-secondary cooperation model is proposed to increase the transmission rate of energy/spectrum constrained users. In the proposed model, a multi-antenna secondary user conducts simultaneous energy harvesting and information forwarding by means of power splitting (PS), and tries to maximize its own transmission rate under the premise of successfully assisting the data delivery of the primary user. After the problem formulation, joint power splitting and beamforming optimization algorithms for decode-and-forward and amplify-and-forward modes are presented, in which we obtain the optimal PS factor and beamforming vectors using a golden search method and dual methods. Simulation results show that the proposed SWIPTbased primary-secondary cooperation schemes can obtain a much higher level of performance than that of non-SWIPT cooperation and non-cooperation schemes.
Xin Chao Liu,Xiao Nan Lu,Qi Zhi Liu,Zhi Long Liu 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4
Water-distilled essential oil from the dried bulbs of Allium chinense (Liliaceae) was analyzed by gaschromatography–mass spectrometry (GC–MS). Eighteen compounds, accounting for 98.4% of the total oil,were identified and the main components of the essential oil of A. chinense were methyl allyl trisulfide(30.7%), dimethyl trisulfide (24.1%), methyl propyl disulfide (12.8%) and dimethyl disulfide (9.6%) followed bymethyl allyl disulfide (3.4%) and methyl propyl trisulfide (3.6%). The essential oil exhibited contact toxicityagainst the booklice (Liposcelis bostrychophila) with an LC50 value of 441.8 μg/cm2 while the two major constituents,dimethyl trisulfide and methyl propyl disulfide had LC50 values of 153.0 μg/cm2 and 738.0 μg/cm2 againstthe booklice, respectively. The essential oil of A. chinense possessed strong fumigant toxicity against the booklicewith an LC50 value of 186.5 μg/l while methyl allyl trisulfide (LC50=90.4 μg/l) and dimethyl trisulfide (LC50=114.2 μg/l) exhibited stronger fumigant toxicity than methyl propyl disulfide (LC50= 243.4 μg/l) and dimethyldisulfide (LC50= 340.8 μg/l) against the booklice. The results indicated that the essential oil and itsmajor constituentshave potential for development into natural insecticides or fumigants for control of insects in stored grains.
Chunping Yu,Yi Zhang,Ning Wang,Wensu Wei,Ke Cao,Qun Zhang,Peiying Ma,Dan Xie,Pei Wu,Biao Liu,Jiahao Liu,Wei Xiang,Xing Hu,Xuewen Liu,Jianfei Xie,Jin Tang,Zhi Long,Long Wang,Hongliang Zeng,Jianye Liu 한국생체재료학회 2022 생체재료학회지 Vol.26 No.1
Background: Circular RNAs (circRNAs) have important functions in many fields of cancer biology. In particular, we previously reported that the oncogenic circRNA, circPRMT5, has a major role in bladder cancer progression. Therapy based on circRNAs have good prospects as anticancer strategies. While anti-circRNAs are emerging as therapeutics, the specific in vivo delivery of anti-circRNAs into cancer cells has not been reported and remains challenging. Methods: Synthesized chrysotile nanotubes (SCNTs) with a relatively uniform length (~ 200 nm) have been designed to deliver an siRNA against the oncogenic circPRMT5 (si-circPRMT5) inhibit circPRMT5. In addition, the antitumor effects and safety evaluation of SCNTs/si-circPRMT5 was assessed with a series of in vitro and in vivo assays. Results: The results showed that SCNTs/si-circPRMT5 nanomaterials prolong si-circPRMT5’s half-life in circulation, enhance its specific uptake by tumor cells, and maximize the silencing efficiency of circPRMT5. In vitro, SCNTs encapsulating si-circPRMT5 could inhibit bladder cancer cell growth and progression. In vivo, SCNTs/si-circPRMT5 inhibited growth and metastasis in three bladder tumor models (a subcutaneous model, a tail vein injection lung metastatic model, and an in situ model) without obvious toxicities. Mechanistic study showed that SCNTs/sicircPRMT5 regulated the miR-30c/SNAIL1/E-adherin axis, inhibiting bladder cancer growth and progression. Conclusion: The results highlight the potential therapeutic utility of SCNTs/si-circPRMT5 to deliver si-circPRMT5 to treat bladder cancer.
Zhi-long Wang,Ming-nian Wang,Dagang Liu 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.12
Convergence-confinement method (CCM) has become a common method for tunnel design due to its simplicity and practicality. The ground reaction curve (GRC), the support characteristic curve (SCC) and the longitudinal deformation profile (LDP) are all considered in this method. One of these is the LDP, which serves as a unique identifier for the location of the tunnel support. The installation point of the tunnel support is one of the most important outcomes of the method. LDP is more impacted by the reinforcement of the tunnel face. However, more existing LDP calculation methods do not consider the reinforcement of the tunnel face. A study on methods for calculating LDP considering the tunnel face reinforcement was carried out to address this constraint of CCM. Firstly, the transformation of tunnel face extrusion deformation and pre-convergence deformation at the tunnel face is realized in accordance with equivalent volume. Then a model of LDP with the tunnel face extrusion deformation as the variable was established considering the tunnel face reinforcement effect. The proposed model's accuracy is assessed in comparison with other typical models. We conclude with parametric analyses concerning the tunnel face reinforcement's effects on the point of support installation, as well as convergence deformation of the tunnel. By taking into account tunnel faces' reinforcement effects, tunnel support design can be more accurate, and the construction costs can be reduced.
Fretting Fatigue Behavior of Ti–6Al–4V and Ti–10V–2Fe–3Al Alloys
Zhi Yan Li,Xiao Long Liu,Guo Qing Wu,Zheng Huang 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.1
The effect of fretting on fatigue performance of different microstructures for titanium alloy was studied using a high-frequencypush–pull fatigue testing machine. Both plain and fretting fatigue curves were obtained for comparative analysis of the frettingeffect on fatigue performance of the different titanium alloy. The result shows that the strength, plain fatigue of Ti6Al4Vtitanium is lower than those of Ti1023 titanium. But the fretting fatigue of Ti6Al4V titanium is higher under each contactstress. The fatigue source depth of Ti1023 alloy is greater than Ti6Al4V alloy. Hardening of Ti1023 alloy is more seriousafter fretting. The wear mechanism of two titanium alloys is different, Ti1023 titanium alloy is more sensitive to fretting wear.