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Uniaxial Eccentric Load Behavior of L-CFSST Composite Column
Zhen Wang,Zhe Liu,Xingbo Wang,Xuejun Zhou,Zhoutai Wang,Guodong Xian 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.5
This study presents the mechanical behavior of L-shaped concrete-filled square steel tube (L-CFSST) composite columns under uniaxial eccentric compression. Nine specimens were tested and the effects of eccentricity, steel tube thickness, steel yield strength, and slenderness ratio were investigated regarding failure modes, ultimate load, load-lateral displacement curves, and load-strain curves. Test results showed that, under uniaxial eccentric load, these columns showed favorable mechanical properties and steel tubes showed strong cooperative working performance. There were two types of failure modes for these columns: local buckling of the steel tube or overall bending of the specimen. ABAQUS was used to simulate the loading process and the accuracy of the numerical simulation results was verified by comparing failure characteristics, load-displacement curves, and ultimate bearing capacity with the test results. The N/Nu-M/Mu curves of the columns under uniaxial eccentric load with different parameters were simulated by the finite element method. A simplified formula for predicting the N/Nu-M/Mu relationship was established based on the finite element analysis results, which might be a workable approach for designing L-CFSST composite columns in engineering practice.
Liuqiang Wang,Zhen Li,Chao Wang,Deyin Wang,Yucheng Wang,Mengzhu Lu 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6
Peroxiredoxins (Prxs) are ubiquitous thiol-specificantioxidant enzymes that are critically involved in cell defenseand protect cells from oxidative damage. In this study, aputative Type II Prx (ThPrx1) was identified and characterizedfrom Tamarix hispida. The expression of ThPrx1 is highlyinduced in response to hydrogen peroxide (H2O2) and methylviologen (MV) stresses. When expressed ectopically, ThPrx1showed enhanced tolerance against oxidative stress in yeastand Arabidopsis. In addition, transgenic Arabidopsis plantsoverexpressing ThPrx1 displayed improved seedling survivalrates and increased root growth and fresh weight gain underH2O2 and MV treatments. Moreover, transgenic Arabidopsisplants showed decreased accumulation of H2O2, superoxide(O2•−) and malondialdehyde (MDA), increased superoxidedismutase (SOD) activity compared to wild-type (WT) plantsunder oxidative stress. Moreover, transgenic plants maintainedhigher photosynthesis efficiency and lower electrolyte leakagerates than that of WT plants under stress conditions. Theseresults clearly indicated that ThPrx1 plays an important rolein cellular redox homeostasis under stress conditions, leadingto the maintenance of membrane integrity and increasedtolerance to oxidative stress.
Tyrosine nitration site specificity identified by LC/MS innitrite-modified collagen type IV
Zhen Wang,David C. Paik,James P. Dillon,Elizabeth R. Gaillard 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.1
Non-enzymatic nitrite induced collagen cross-link-ing results in changes reminiscent of age-related damage and parallels the well-known model system, non-enzymatic glycation. We have recently ob-served that nitrite modification of basement mem-brane proteins can induce deleterious effects on overlying retinal pigment epithelial cells in studies relevant to age-related macular degeneration. The 3-nitro-tyrosine (3-NT) as a product of the reaction and to identify the site specificity of nitration in collagen IV, a major component of basement mem-branes. Human collagen type IV was modified via incubation with 200 mM NaNO2 (pH = 7.38) for one week at 37oC. The modified protein was prepared in 2 different ways, including acid hydrolysis and trypsin digestion for site specificity determination. The samples were analyzed by LC/MS using a C12 RP column. Site specificity was determined from tan-dem MS/MS data utilizing TurboSEQUEST software and the Swiss-Prot sequence database. 3-NT was detected in protein digests and acid hydrolysates of nitrite modified collagen IV. Positive identification with standard 3-NT was confirmed by identical Rt, λmax= 279 nm and 355 nm, and m/z= 227. Analyses of tryptic digests identified four sites of tyrosine ni-tration, α1(IV)Y348, α1(IV)Y534, α2(IV)Y327, and α2(IV)-Y1081. These sites are located in the triple- helical region of the protein and provide clues regarding potential sites for nitrite modification in collagen type IV.
Wang, Zhen-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2
This study is aimed to investigate the role of paired boxed gene 1 (PAX1) methylation analysis by methylation-sensitive high-resolution melting (MS-HRM) in the detection of high grade lesions in atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (ASC-H) and compared its performance with the Hybrid Capture 2 (HC2) human papillomavirus (HPV) test. In our study, 130 cases with a diagnosis of ASC-H from the cervical cytological screening by Thinprep cytologic test (TCT) technique were selected for triage. Their cervical scrapings were collected and evaluated by using PAX1 methylation analysis (MS-HRM) and high-risk HPV DNA test (HC2), followed by colposcopy and cervical biopsy. Chi-square test were used to test the differences of PAX1 methylation or HPV infection between groups. In the detection of CIN2+, the sensitivity, specificity, the PPV, NPV and the accuracy of PAX1 MS-HRM assay and high-risk HPV (HR-HPV) tests were respectively 80.6% vs 67.7%, 94.9% vs 54.5%, 83.3%, vs 31.8%, 94.0% vs 84.4%, and 91.5% vs 57.7%. The PAX1 MS-HRM assay proved superior to HR-HPV testing in the detection of high grade lesions (CIN2+) in ASC-H. This approach could screen out the majority of high grade lesion cases of ASC-H, and thus could reduce the referral rate to colposcopy.
Zhen Wang,Bin Wu,Guoshan Xu,Yong Ding,Oreste S. Bursi 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.6
Real-Time Hybrid Simulation (RTHS) is a novel approach conceived to evaluate dynamic responses of structures with parts of a structure physically tested and the remainder parts numerically modelled. In RTHS, delay estimation is often a precondition of compensation; nonetheless, system delay may vary during testing. Consequently, it is sometimes necessary to measure delay online. Along these lines, this paper proposes an online delay estimation method using least-squares algorithm based on a simplified physical system model, i.e., a pure delay multiplied by a gain reflecting amplitude errors of physical system control. Advantages and disadvantages of different delay estimation methods based on this simplified model are firstly discussed. Subsequently, it introduces the least-squares algorithm in order to render the estimator based on Taylor series more practical yet effective. As a result, relevant parameter choice results to be quite easy. Finally in order to verify performance of the proposed method, numerical simulations and RTHS with a buckling-restrained brace specimen are carried out. Relevant results show that the proposed technique is endowed with good convergence speed and accuracy, even when measurement noises and amplitude errors of actuator control are present.