Research on the Development Strategy of China’s Semiconductor Industry under Sino-US Science and Technology Relations

Li Yuying,Sun Liping 한중경제문화학회 2023 한중경제문화연구 Vol.24 No.-

Purpose - In 2022, the United States introduced Creative Helpful Incentives to Produce Semiconductors for America Act, which strengthened the export control on China’s chips, semiconductors, and other products. The scientific and technological relation between China and the United States is in a tense state, which has a negative impact on the complementary development of technology between China and the United States, as well as the development of China’s semiconductor industry. In the current situation of the scientific and technological relation between China and the United States, this paper hopes to play a certain reference role in the faster and better development of China’s semiconductor industry. Design/Methodology/Approach - This paper uses literature analysis, qualitative analysis and case analysis to elaborate on the general situation of the semiconductor industry and the development process of China’s semiconductor industry under the global semiconductor industry, and analyzes the development, present situation and future of Sino-us scientific and technological relations. Findings - This paper discusses the development problems and opportunities of China’s semiconductor industry under the Sino-US science and technology relations, and puts forward the development strategy of China’s semiconductor industry under the Sino-US science and technology relations. This paper can bring enlightenment to the development of China’s semiconductor industry under the Sino-US science and technology relations. Research Implications - Through the study of the development opportunities and strategies of China’s semiconductor industry under the Sino-US scientific and technological relation, Chinese semiconductor enterprises can better cope with the impact of the United States on the development of China’s semiconductor industry, grasp the development opportunities of the semiconductor industry, and achieve the development goals of the semiconductor industry in the difficult situation.

An Engineered Outer Membrane-Defective Escherichia coli Secreting Protective Antigens against Streptococcus suis via the Twin-Arginine Translocation Pathway as a Vaccine

( Wenyu Li ),( Fan Yin ),( Zixuan Bu ),( Yuying Liu ),( Yongqing Zhang ),( Xiabing Chen ),( Shaowen Li ),( Lu Li ),( Rui Zhou ),( Qi Huang ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.3

Live bacterial vector vaccines are one of the most promising vaccine types and have the advantages of low cost, flexibility, and good safety. Meanwhile, protein secretion systems have been reported as useful tools to facilitate the release of heterologous antigen proteins from bacterial vectors. The twin-arginine translocation (Tat) system is an important protein export system that transports fully folded proteins in a signal peptide-dependent manner. In this study, we constructed a live vector vaccine using an engineered commensal Escherichia coli strain in which amiA and amiC genes were deleted, resulting in a leaky outer membrane that allows the release of periplasmic proteins to the extracellular environment. The protective antigen proteins SLY, enolase, and Sbp against Streptococcus suis were targeted to the Tat pathway by fusing a Tat signal peptide. Our results showed that by exploiting the Tat pathway and the outer membrane-defective E. coli strain, the antigen proteins were successfully secreted. The strains secreting the antigen proteins were used to vaccinate mice. After S. suis challenge, the vaccinated group showed significantly higher survival and milder clinical symptoms compared with the vector group. Further analysis showed that the mice in the vaccinated group had lower burdens of bacteria load and slighter pathological changes. Our study reports a novel live bacterial vector vaccine that uses the Tat system and provides a new alternative for developing S. suis vaccine.

SALT-INDUCED CHLOROPLAST PROTEIN (SCP) is Involved in Plant Tolerance to Salt Stress in Arabidopsis

Yong Zhuang,Yangxuan Liu,Yuxiang Li,Ming Wei,Yuying Tang,Penghui Li,Zhijian Liu,Hui Li,Weizao Huang,Songhu Wang 한국식물학회 2019 Journal of Plant Biology Vol.62 No.6

Soil salinization threats the agricultural productionand food security worldwide. Salt stress induced plantsenescence and chloroplast degradation. However, it remainslargely unknown how the chloroplast-localized proteins affectplant response to salt stress. Here, we characterized a novelgene (At5g39520) in Arabidopsis, which is induced by saltstress and encodes a chloroplast-localized protein. Thus, thisgene was named SALT-INDUCED CHLOROPLAST PROTEIN(SCP). A T-DNA insertion mutant of SCP gene (scp-1)showed the enhanced tolerance to salt stress, as indicated bythe increased survival rates, fresh weights and chlorophyllcontents compared with wild type plants under salt treatment. Salt-induced leaf senescence was also delayed in scp-1 mutant. The scp-1 complementation line and SCP overexpressionlines displayed the hypersensitivity to salt stress. The qRTPCRanalysis indicated that the transcripts of CHLOROPLASTVESICULATION (CV), which mediates stress-inducedchloroplast degradation, were altered in scp-1 mutant andSCP overexpression lines. Taken together, our results suggestthat SCP gene plays a negative role in response to salt stress andhas potential application for genetic modification of improvingplant tolerance to salt stress.

Identification of Plasma Biomarkers in Drug-Naïve Schizophrenia Using Targeted Metabolomics

Qiao Su,Fuyou Bi,Shu Yang,Huiming Yan,Xiaoxiao Sun,Jiayue Wang,Yuying Qiu,Meijuan Li,Shen Li,Jie Li 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.9

Objective Schizophrenia (SCZ) is a severe psychiatric disorder with unknown etiology and lacking specific biomarkers. Herein, we aimed to explore plasma biomarkers relevant to SCZ using targeted metabolomics. Methods Sixty drug-naïve SCZ patients and 36 healthy controls were recruited. Psychotic symptoms were assessed using the Positive and Negative Syndrome Scale. We analyzed the levels of 271 metabolites in plasma samples from all subjects using targeted metabolomics, and identified metabolites that differed significantly between the two groups. Then we evaluated the diagnostic power of the metabolites based on receiver operating characteristic curves, and explored metabolites associated with the psychotic symptoms in SCZ patients. Results Twenty-six metabolites showed significant differences between SCZ patients and healthy controls. Among them, 12 metabolites were phosphatidylcholines and cortisol, ceramide (d18:1/22:0), acetylcarnitine, and γ-aminobutyric acid, which could significantly distinguish SCZ from healthy controls with the area under the curve (AUC) above 0.7. Further, a panel consisting of the above 4 metabolites had an excellent performance with an AUC of 0.867. In SCZ patients, phosphatidylcholines were positively related with positive symptoms, and cholic acid was positively associated with negative symptoms. Conclusion Our study provides insights into the metabolite alterations associated with SCZ and potential biomarkers for its diagnosis and symptom severity assessment.

Lentivirus-Mediated Short-Hairpin RNA Targeting Protein Phosphatase 4 Regulatory Subunit 1 Inhibits Growth in Breast Cancer

Yuying Qi,Tinghui Hu,Kai Li,Renqing Ye,Zuodong Ye 한국유방암학회 2015 Journal of breast cancer Vol.18 No.3

Purpose: Protein phosphatase 4 regulatory subunit 1 (PP4R1), as an interaction partner of the catalytic serine/threonine-protein phosphatase 4 catalytic subunit has been shown to involve in cellular processes and nuclear factor κB signaling. However, the functions of PP4R1 in human breast cancers remain unclear. This study is designed to explore the effect of PP4R1 knockdown on the biological characteristics of breast cancer cells. Methods: A lentivirus-mediated short hairpin RNA (shRNA) was designed to knockdown the expression of PP4R1 in ZR-75-30 breast cancer cells. The efficiency of lentivirus-mediated shRNA infection was determined using fluorescence microscopy to observe lentivirus-mediated green fluorescent protein expression and confirmed to be over 80%. PP4R1 expression in infected ZR-75-30 cells was detected by quantitative real-time polymerase chain reaction and western blot analysis. Cell proliferation and colony formation ability were measured by 3-(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and colony formation assay, respectively. Flow cytometry was used to measure cell cycle progression and cell apoptosis. In addition, apoptosis makers, including poly-ADP-ribose polymerase (PARP) and Caspase-3, were investigated in PP4R1- silenced ZR-75-30 cells by western blot assay. Results: We successfully constructed lentivirus-mediated shRNA to target PP4R1 in ZR-75-30 cells. MTT assay and colony formation assay showed the loss of PP4R1 suppressed the proliferation of ZR-75-30 cells. Flow cytometry analysis indicated cell cycle arrest and increased cell apoptosis in PP4R1 knockdown cells. Further, the apoptosis response in cells depleted of PP4R1 was illustrated by downregulation of PARP and upregulation of Caspase- 3. Conclusion: Our results suggest that PP4R1 could promote breast cancer cell proliferation and might play a vital role in breast cancer occurrence.

YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44

Li Weijian,Chen Gaohuang,Feng Zhenyu,Zhu Baoyi,Zhou Lilin,Zhang Yuying,Mai Junyan,Jiang Chonghe,Zeng Jianwen 한국유전학회 2021 Genes & Genomics Vol.43 No.12

Background Prostate cancer (PCa) is one of the most common malignancies in men. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa. Objective This study explored whether YTHDF1 could regulate TRIM44 in PCa cells. Methods By querying the TCGA database, we evaluated YTHDF1 expression in PCa, the OS and DFS of YTHDF1, and the correlation between YTHDF1 and TRIM44 in PCa. We constructed vectors to interfere with YTHDF1 expression and overexpress TRIM44 to examine the role of YTHDF1 and TRIM44 in PCa cells. Diferentially expressed mRNAs were identifed by mRNA sequencing. The levels of YTHDF1, TRIM44, LGR4, SGTA, DDX20, and FZD8 were measured by qRT-PCR and WB was used to determine YTHDF1 and TRIM44 expression. A CCK-8 assay was used to assess cell proliferation. A Transwell chamber assay was used measure cell migration and invasion ability. Results YTHDF1 was highly expressed in both Pca tissues and cells. PCa patient prognosis with high YTHDF1 expression was relatively poor. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verifed that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44. Conclusions YTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44.

Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus

Yuying Cao,Li Yuan,Shunli Yang,Youjun Shang,Bin Yang,Zhizhong Jing,Hui-Chen Guo,Shuanghui Yin 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.5

Background: Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry. Objectives: This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples. Methods: A spbELISA method was developed based on the recombinant E2 expressed in Escherichia coli. The specificity of this established spbELISA method was evaluated using reference serum samples positive for antibodies against other common infectious diseases. The stability and sensitivity were evaluated using an accelerated thermostability test. Results: The spbELISA successfully detected the antibody levels in swine vaccinated with the C-strain of CSFV. In addition, the detection ability of spbELISA for CSFV antibodies was compared with that of other commercial ELISA kits and validated using an indirect immunofluorescence assay. The results suggested that the spbELISA provides an alternative, stable, and rapid serological detection method suitable for the large-scale screening of CSFV serum antibodies. Conclusions: The spbELISA has practical applications in assessing the vaccination status of large pig herds.

Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus

Cao, Yuying,Yuan, Li,Yang, Shunli,Shang, Youjun,Yang, Bin,Jing, Zhizhong,Guo, Huichen,Yin, Shuanghui 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.3

Background: Classical swine fever (CSF) is a severe infectious disease of pigs that causes significant economic losses to the swine industry. Objectives: This study developed a solid-phase blocking enzyme-linked immunosorbent assay (spbELISA) method for the specific detection of antibodies against the CSF virus (CSFV) in porcine serum samples. Methods: A spbELISA method was developed based on the recombinant E2 expressed in Escherichia coli. The specificity of this established spbELISA method was evaluated using reference serum samples positive for antibodies against other common infectious diseases. The stability and sensitivity were evaluated using an accelerated thermostability test. Results: The spbELISA successfully detected the antibody levels in swine vaccinated with the C-strain of CSFV. In addition, the detection ability of spbELISA for CSFV antibodies was compared with that of other commercial ELISA kits and validated using an indirect immunofluorescence assay. The results suggested that the spbELISA provides an alternative, stable, and rapid serological detection method suitable for the large-scale screening of CSFV serum antibodies. Conclusions: The spbELISA has practical applications in assessing the vaccination status of large pig herds.

Stable Amorphous Bis(diarylamino)biphenyl Derivatives as Hole-Transporting Materials in OLEDs

Zhanfeng Li,Zhaoxin Wu,Wen Fu,Dongdong Wang,Peng Liu,Bo Jiao,Xiaoli Lei,Guijiang Zhou,Yuying Hao 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.5

Stable bis(diarylamino)biphenyl derivatives N4,N4'-di(biphenyl-4-yl)-N4,N4'-bis(2- methylbiphenyl-4-yl)biphenyl-4,4'-diamine (TPD-Ph) and N4,N4'-bis(2',4'-difluoro- 2-methylbiphenyl-4-yl)-N4,N4'-bis(2',4'-difluorobiphenyl-4-yl)biphenyl-4,4'-diamine (TPD-(2,4)-F) were synthesized and characterized. High thermal stability (Td ≥ 480°C and Tg ≥ 108°C) in combination with reversible oxidation process render the both promising candidates as hole-transporting materials for organic light-emitting devices. The device with structure of ITO/TPD-Ph/Alq3/LiF/Al presented the highest current efficiency of 4.2 cd A−1 and good operational stability even under the high current density of 350 mA cm−2, which outperformed TPD or NPB-based devices.

Effect of FTY-720 on Pulmonary Fibrosis in Mice via the TGF-β1 Signaling Pathway and Autophagy

Jin Yuying,Liu Weidong,Gao Ge,Song Yilan,Liu Hanye,Li Liangchang,Zhou Jiaxu,Yan Guanghai,Cui Hong 한국응용약물학회 2023 Biomolecules & Therapeutics(구 응용약물학회지) Vol.31 No.4

We investigated whether FTY-720 might have an effect on bleomycin-induced pulmonary fibrosis through inhibiting TGF-β1 pathway, and up-regulating autophagy. The pulmonary fibrosis was induced by bleomycin. FTY-720 (1 mg/kg) drug was intraperitoneally injected into mice. Histological changes and inflammatory factors were observed, and EMT and autophagy protein markers were studied by immunohistochemistry and immunofluorescence. The effects of bleomycin on MLE-12 cells were detected by MTT assay and flow cytometry, and the related molecular mechanisms were studied by Western Blot. FTY-720 considerably attenuated bleomycin-induced disorganization of alveolar tissue, extracellular collagen deposition, and α-SMA and E-cadherin levels in mice. The levels of IL-1β, TNF-α, and IL-6 cytokines were attenuated in bronchoalveolar lavage fluid, as well as protein content and leukocyte count. COL1A1 and MMP9 protein expressions in lung tissue were significantly reduced. Additionally, FTY- 720 treatment effectively inhibited the expressions of key proteins in TGF-β1/TAK1/P38MAPK pathway and regulated autophagy proteins. Similar results were additionally found in cellular assays with mouse alveolar epithelial cells. Our study provides proof for a new mechanism for FTY-720 to suppress pulmonary fibrosis. FTY-720 is also a target for treating pulmonary fibrosis.