Effect of Osmotic Stress on Human Red Cell Rheology: Cell Deformability, Aggregability and Blood Viscosity

Ku, Yun-Hee,Shin, Se-Hyun,Suh, Jang-Soo Biomedical Engineering Society for Circulation 2006 International Journal of Vascular Biomedical Engin Vol.4 No.2

The present study investigated the effects of the osmotic environment on the rheological properties of erythrocytes and their suspensions. In an iso-osmotic medium, erythrocytes forming a biconcave discocyte under resting conditions, exhibited high deformability. In a low-osmotic medium, the deformability of erythrocytes, which swelled and exhibited a spherical shape, significantly decreased at a high shear stress and the high-shear viscosity of the cell suspension was slightly higher than that of normal blood. Hyper-osmotic stress, however, which caused to form echinocytes, decreased cell deformability but exhibited smaller viscosity in low shear rates than iso-osmotic blood viscosity. These results showed a close relation with the aggregability measurements, in that hypertonic blood showed lower aggregability than the hypotonic and isotonic RBC suspensions. These findings indicate that the physicochemical environment has a strong influence on the rheological properties of the erythrocyte and its suspensions.

제2형 당뇨병환자에서 유산소 운동 강도에 따른 인슐린저항성의 변화

구윤희 ( Yun Hyi Ku ),구보경 ( Bo Kyung Koo ),안희정 ( Hee Jung Ahn ),정지연 ( Ji Yun Jeong ),석희금 ( Hee Geum Seok ),김호철 ( Ho Chul Kim ),한경아 ( Kyung Ah Han ),민경완 ( Kyung Wan Min ) 대한당뇨병학회 2009 Diabetes and Metabolism Journal Vol.33 No.5

연구배경: 운동은 주로 동맥경화증 및 인슐린저항성으로부터 보호하는 작용을 통해 사망률을 감소시키는 데에 도움이 되는 것으로 알려져 있다. 이번 연구에서는 제2형 당뇨병환자에서 어떤 강도의 운동이 염증을 완화하고, 내피 세포 기능을 개선시키며 인슐린저항성을 호전시키는 데에 더욱 도움이 되는지 알아보고자 하였다. 방법: 총 59명의 비만한 여성 2형 당뇨병환자가 대조군(CG, N=18), 중강도 운동군(MEG, N=17), 고강도 운동군(VEG, N=14)으로 randomly 배정되어, 가속계로 모니터하며 12주간 운동 요법을 시행하였다. 연구시작시점, 12주 운동 종료 시점, 연구시작으로부터 1년 시점에 체중, 전산화 단층 촬영을 이용하여 복부지방, 피하지방, 내장지방을 측정하였으며, 혈장 hs-CRP, IL-6, 인슐린감수성을 측정하였다. 결과: 평균 연령은 54±7세, 체질량지수는 26.9±2.5 kg/m2이었다. 운동 요법 동안 두 운동군은 운동 칼로리로 각각 488.6±111.9 kcal/day, 518.8±104.1 kcal/day를 사용하였다. 체질량지수, 복부지방, 피하지방은 두 운동군에서(ΔBMI: -1.1±0.7, -0.8±0.5, ΔTF: -4,647±3,613 mm2, -2,577±2,872 mm2, ΔSF: -2,057±2,021 mm2, -1,141±1,825 mm2, respectively) 대조군에 비해 유의하게 감소하였으나(P<0.01), hs-CRP, IL-6 및 FMD는 변화 없었다. 인슐린감수성은 고강도 운동군에서만 감소하였으며, 내장지방은 중강도 운동군에서만 감소하였다. 1년 추적관찰 결과, 이러한 차이는 모두 소실되는 경향을 보였다. 결론: 제2형 당뇨병환자에서 체중 및 체지방 감소를 동반한 고강도 운동은 3개월간의 intervention을 통해 전신 염증수치나 내피 세포 기능의 변화 없이 인슐린감수성 개선 효과를 나타냈다. Background: Exercise offers protection against atherosclerosis and insulin resistance. We evaluated the benefits of exercise at different levels of intensity for ameliorating inflammation, endothelial dysfunction, and insulin resistance in a sample of type 2 diabetic subjects. Methods: Fifty-nine overweight women with type 2 diabetes were randomly assigned to control (CG, N=18), moderate-intensity exercise (MEG, N=17), and vigorous-intensity exercise (VEG, N=14) groups. Patients in the two experimental groups completed a 12-week exercise program, with their exercise activities monitored by accelerometers. We assessed the patients` body weights, total abdominal fat (TF), subcutaneous fat (SF) and visceral fat (VF) via computed tomography, measurements of plasma levels of hs-C-reactive protein (hs-CRP) and interleukin-6 (IL-6), assessment of endothelial function by brachial artery flow-mediated dilation (FMD), and evaluation of insulin sensitivity by insulin tolerance tests, at baseline, at the end of the 12-week interventions, and one year after initiation of the study. Results: At baseline, the average age of all subjects was 54±7 years, and average body mass index (BMI) was 26.9±2.5 kg/m2. During the intervention, patients in the MEG and VEG groups expended comparable amounts of activity-related calories (488.6±111.9 kcal/day, 518.8±104.1 kcal/day, respectively). Although BMI, TF, and SF decreased similarly in the MEG and VEG groups (ΔBMI: -1.1±0.7, -0.8±0.5, ΔTF: -4,647±3,613 mm2, -2,577±2,872 mm2, ΔSF: -2,057±2,021 mm2, -1,141±1,825 mm2, respectively), compared to control (P<0.01), hs-CRP, IL-6, and FMD remained constant in both exercise groups even after completion of the 12-week exercise intervention. Insulin sensitivity improved only in patients subjected to vigorous exercise (VEG). Visceral fat loss was observed only in patients subjected to moderate exercise (MEG). At one-year follow up, these values had all returned to baseline. Conclusion: Exercise vigorous enough to result in significant weight and fat reduction did not ameliorate inflammation and endothelial dysfunction as measured at the end of a 12-week exercise intervention, nor did it result in sustained improvements in insulin sensitivity in type 2 diabetic subjects. (Korean Diabetes J 33:401-411, 2009)

초등학생용 생활역량 진단검사 개발 및 타당화 연구

윤명희 ( Yun Myung Hee ),구경희 ( Ku Kyung Hee ) 한국초등교육학회 2016 초등교육연구 Vol.29 No.3

본 연구의 목적은 초등학생들이 성인이 되어 자신의 삶을 성공적으로 주도해나갈 수 있는 생활인으로 성장하는데 필요한 역량을 구체적으로 진단할 수 있는 초등학생용 생활역량 진단척도를 개발하는데 있다. 이를 위해 `생활역량모델`에 근거하여 5, 6학년을 대상으로 예비검사와 본검사를 실시하여 자료를 수집하였다. 본 연구 과정에서 개발된 초등학생 생활역량 진단검사는 자기조절역량, 자기개발역량, 학습역량, 대인관계역량, 민주시민역량, 문제상황대처역량의 6개 하위 생활역량과 이를 구체적으로 함양할 수 있는 16개 생활기술, 각 기술별 5문항인 총 80문항으로 구성되었다. 전문가에 의한 내용타당도 검증과 요인부하량 및 상관관계 분석을 통해 구인타당도를 검증하였고, 경로모형의 표준화계수 산출 및 적합도 검증을 통해 모델의 타당성을 검증하였다. 6개 하위 생활역량의 신뢰도는 0.83∼0.91로 나타났으며, 전체 검사도구의 신뢰도는 0.97로 나타났다. 진단검사는 생애발달적 차원에서 민주시민으로 성장하는데 필요한 역량이나 기술을 어려서부터 함양시킬 수 있는 기초자료로 활용될 수 있을 것이다. The purpose of this study is to develop the Life Competency Test for elementary school students, which can be used for growing as an individual who can lead successfully his/her own life. For this, items were developed from literature review and Life Competency Model. 226 students for pretest and 584 students of 5th and 6th grade were participated for this study. Through test development processes, the Life Competency Test with 6 sub-life competencies, 16 life skills and 80 items was developed. 6 sub-life competencies are as follows: self regulation, self development, learning, interpersonal relationship, democratic citizenship, and coping with problematic situations. The coefficients of Cronbach α and construct validity were high, and suitability indexes of item factor structure were high. Students can get informations about their life competencies easily through this test, furthermore, teachers or parents can provide educational programs to enhance student`s deficient competencies or life skills. Also, the collected data from Life Competency Tests can be used as basic data to develop programs and to make policies in elementary schools or fields of youth.

월드컵 스폰서십과 매복마케팅: KTF의 2002 월드컵과 2006 월드컵 기업PR 사례연구

구윤희 ( Yun Hee Ku ),심재철 ( Jae Chul Shim ) 한국PR학회 2007 PR연구 Vol.11 No.1

This study compares KTF (Korea Telecom Freetell) official sponsorship of the Korea-Japan World Cup in 2002 with its ambush marketing of the 2006 FIFA World Cup in Germany. We have researched KTF`s sports marketing campaigns and compared the two periods of World Cup sponsorships in 2002 and 2006. This case study provides the rare opportunity since the one Korean mobile company sponsored the World Cup sequentially in a different way. We have examined the corporate public relations, specifically by focusing on the company`s different preparation, process and the end results in 2002 and 2006. In-depth interviews were conducted to obtain strategic inside information from the company. Also, any news articles related to KTF and the World Cup events from Korean newspapers were qualitatively content-analyzed. We found that KTF campaigned effectively by using a monopolistic position as an official sponsor of the World Cup in 2002. Nevertheless, there was an in-house criticism that KTF`s official sponsorship was not effective compared with its rival company, SK Telecom`s ambush marketing in the same period. Yet, KTF`s ambush marketing in 2006 had great success since it was based on the long-term planning of KTF`s corporate communication. KTF took over SKT`s ambush marketing position in 2006 by winning over the popularity of the Red Devils, red-clad cheerers of the Korean national soccer team. Based on these findings, we discuss official sponsorship with ambush marketing, elaborate a model of corporate sports marketing campaigns, and emphasize long-term planning and ethical performance.

CSR 진정성 효과 연구 -CSR에 대한 기업의 실제 동기와 표현 동기 일치 여부를 중심으로

구윤희 ( Yun Hee Ku ),윤영민 ( Youngmin Yoon ),이헌율 ( Hun Yul Lee ) 한국PR학회 2015 PR연구 Vol.19 No.4

This study was conducted to explore the effect of a firm``s CSR authenticity toward response of the public by comparing the attitudes toward CSR, the attitudes toward corporations, and activation of persuasion knowledge. CSR authenticity was manipulated with the consistency of the firm``s actual motive and stated motive. This study expected that self-centered stated motive with high CSR authenticity would get more positive response from public than those of other-centered stated motive with low CSR authenticity. An experimental result indicated that there was no significant difference between those two groups influenced by CSR authenticity. Although the CSR authenticity did not make any difference to activate persuasion knowledge, once the public``s persuasion knowledge is activated, there was a negative effect on publics`` attitudes toward CSR and corporations. This study also found that other-centered CSR motive gets more positive response than self-centered motive under a high CSR authenticity condition. Based on the result, we conducted supplementary analysis with reassignment of the firm``s actual CSR motives data. The result showed that there was a negative effect on attitudes and an activation of persuasion knowledge when the actual motive was self-centered. Meaning, a firm``s actual motive have more positive effect on response of the public regardless of a firm``s CSR authenticity.

근거 기반의 실제(Evidence-Based Practice)를 구축하기 위한 질적 지표 및 로드맵을 통한 분석 : 국내의 긍정적 행동지원 전력 연구들을 중심으로

신윤희 ( Yun Hee Shin ),윤주연 ( Ju Yeon Yun ),구현진 ( Hyun Jin Ku ),구원옥 ( Won Ok Gu ),최미향 ( Mi Hyang Choi ),장수정 ( SU Jung Jang ),김경서 ( Kyong Seo Kim ) 한국정서ㆍ행동장애아교육학회 2009 정서ㆍ행동장애연구 Vol.25 No.3

Evening Chronotype Is Associated With Metabolic Disorders and Body Composition in Middle-Aged Adults

Yu, Ji Hee,Yun, Chang-Ho,Ahn, Jae Hee,Suh, Sooyeon,Cho, Hyun Joo,Lee, Seung Ku,Yoo, Hye Jin,Seo, Ji A,Kim, Sin Gon,Choi, Kyung Mook,Baik, Sei Hyun,Choi, Dong Seop,Shin, Chol,Kim, Nan Hee Issued for the Endocrine Society by the Williams & 2015 The Journal of clinical endocrinology & metabolism Vol.100 No.4

Advantages of In Vitro Cytotoxicity Testing by Using Human Hematoma Cell in Comparison with Mouse Fibroblast 3T3 for the Prediction of Hepatotoxicity

So-ryeon Hwang(So-ryeon Hwang),Seong-Yun Hwang(Seong-Yun Hwang),Hee Yi(Hee Yi),Ji-Hyun Bang(Ji-Hyun Bang),Yong-sang Kim(Yong-sang Kim),Hyun-ok Ku(Hyun-ok Ku),Byung-suk Jeon(Byung-suk Jeon) 한국예방수의학회 2019 한국예방수의학회 학술대회자료집 Vol.2018 No.-

아동의 장난감 선택에 영향을 주는 제 변인에 관한 연구 : 서울 시내 유치원 아동을 중심으로

구윤모,김혜경,유소현,유은영,이승희,임성은,차주연,홍기영 연세대학교 생활과학대학 1988 婦學 Vol.21 No.-

The purpose of this study was to investigate the relationship between ⑴ social status and selection of toys, ⑵ sibling order and selection of toys and ⑶ sex and toy preference. The subjects were 97 mothers of preschool children. A questionnaire was devised and used for the collection of data. The data were analyzed with Chi-square. The results were as follows: ⑴ There was a significant difference in selection of toys according to the socio-economic status of children. ⑵ There was no significant difference in selection of toys according to sibling order of the child. ⑶ There were significant differences between boys and girls in toy preference.

Inactivation of Listeria monocytogenes and Campylobacter jejuni in Chicken by Aqueous Chlorine Dioxide Treatment

Yun-Hee Hong,Gyeong-Ju Ku,Min-Ki Kim,Kyung Bin Song 한국식품영양과학회 2007 Preventive Nutrition and Food Science Vol.12 No.4

Aqueous chlorine dioxide (ClO₂) treatment was used for the inactivation of Listeria monocytogenes and Campylobacter jejuni in chicken. Chicken breasts and legs were inoculated with 8~9 log CFU/g of Listeria monocytogenes and Campylobacter jejuni, respectively, and then treated with 0, 50, and 100 ppm of ClO₂ solution. Aqueous ClO₂ treatment decreased the populations of the pathogenic bacteria on the chicken samples. One hundred ppm ClO₂ treatment on the chicken breast and leg reduced the populations of Listeria monocytogenes and Campylobacter jejuni by 0.61~1.93 and 0.99~1.21 log CFU/g, respectively. Aqueous ClO₂ treatment affected the microbial growth during storage at 4oC by decreasing the initial microbial populations. These results clearly suggest that aqueous ClO₂ treatment should be useful in improving the microbial safety of chicken during storage and extending the shelf life.