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        A Pleiotropic Phenotype is Associated with Altered Endogenous Hormone Balance in the Developmentally Stunted Mutant (dsm1)

        Hui-Fang Zhao,Kai Qiu,Guo-Dong Ren,Yong Zhu,Ben-Ke Kuai 한국식물학회 2010 Journal of Plant Biology Vol.53 No.1

        A developmentally stunted mutant (dsm1) of Arabidopsis, isolated from an EMS mutant screen, had a pleiotropic phenotype, including repressed germination,retarded growth, delayed flowering, and impaired fertility. Additionally, dsm1 had a lifespan of approximately 160 days, which was more than twice the lifespan of the wild type (Col-0). Fine morphological and anatomical characters, such as the shoot apical meristem, root apical meristem, seed shape, and seed surface, were obviously altered in dsm1. We found that both abscisic acid and zeatin riboside levels were significantly greater in dsm1 than in Col-0 at all stages of development, while the levels of indole-3-acetic acid and gibberellins varied by age. The expressions of some abscisic acid-related genes were higher in dsm1 than in Col-0. These data indicate that DSM1 may play a general role in plant growth and development.

      • SCIESCOPUSKCI등재
      • KCI등재

        Effect of Ultrasonic Vibration Tensile on the Mechanical Properties of High-Volume Fraction SiCp/Al Composite

        Dao-Hui Xiang,Zhi-Meng Zhang,Bang-Fu Wu,Hao-Ren Feng,Zhan-Li Shi,Bo Zhao 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.11

        Silicon carbide particle-reinforced aluminum matrix composite has been widely used in the military and aerospace industry due to its special performance; however, there remain many problems in processing. The present paper introduces an ultrasonic vibration tensile device with a view to investigating an ultrasonic vibration tensile specimen. The results show that there are three major stages in the change in stress of the material under ultrasonic vibration: the ultrasonic stress superposition effect, softening effect, and Hall–Petch strengthening effect, these three effects occupy different proportions in different tensile stages. In addition, increasing the frequency of ultrasonic vibration increased the degree of stress reduction. Increasing the ultrasonic vibration amplitude reduced the fracture strength of the material. Comparison of the fracture morphology shows that the conventional condition was mainly interfacial peeling of SiC particles, and cleavage of the fracture occurred under ultrasonic vibration conditions.

      • KCI등재

        Exploring the Key Genes and Pathways of Osteoarthritis in Knee Cartilage in a Rat Model Using Gene Expression Profiling

        Yi-Ming Ren,Xin Zhao,Tao Yang,Yuan-Hui Duan,Yun-Bo Sun,Wen-Jun Zhao,Meng-Qiang Tian 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.6

        Purpose: To compare differentially expressed genes (DEGs) mediating osteoarthritis (OA) in knee cartilage and in normal kneecartilage in a rat model of OA and to identify their impact on molecular pathways associated with OA. Materials and Methods: A gene expression profile was downloaded from the Gene Expression Omnibus database. Analysis ofDEGs was carried out using GEO2R. Enrichment analyses were performed on the Gene Ontology (GO) and Kyoto Encyclopedia ofGenes and Genomes pathway using the Search Tool for the Retrieval of Interacting Genes database (http://www.string-db.org/). Subsequently, the regulatory interaction network of OA-associated genes was visualized using Cytoscape software (version 3.4.0;www.cytoscape.org). Results: In the gene expression profile GSE103416, a total of 99 DEGs were identified. Among them, 76 DEGs (76.77%) were overexpressed,and the remaining 23 DEGs (23.23%) were underexpressed. GO and pathway enrichment analyses of target geneswere performed. Using gene-gene interaction network analysis, relevant core genes, including MET, UBB, GNAI3, and GNA13,were shown to hold a potential relationship with the development of OA in cartilage. Using quantitative real-time PCR, the Gna13/cGMP-PKG signaling pathway was identified as a potential research target for therapy and for further understanding the developmentof OA. Conclusion: The results of the present study provide a comprehensive understanding of the roles of DEGs in knee cartilage in relationto the development of OA.

      • KCI등재

        Finite Element and Experimental Analysis of Ultrasonic Vibration Milling of High-Volume Fraction SiCp/Al Composites

        Zhan-Li Shi,Dao-Hui Xiang,Hao-Ren Feng,Bang-Fu Wu,Zhi-Meng Zhang,Guo-Fu Gao,Bo Zhao 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.10

        SiCp/Al composites have been widely used in many fields due to their excellent mechanical properties. However, the addition of reinforced phase SiC particles makes the overall properties of the composites hard and brittle, which brings great challenges to milling. Ultrasonic vibration-assisted processing technology has great advantages in processing hard and brittle materials. However, the process of rupture of SiC particles cannot be effectively observed during the test processing, and a large number of tests increase the cost of the test. The combination of finite element analysis and experiment was used to study the machining performance of High-volume fraction SiCp/Al composites in longitudinal-torsional ultrasonicassisted milling (LTUAM), and its feasibility was evaluated by comparing with conventional Milling (CM). By analyzing the trajectories of cutting edges in ultrasonic-assisted milling, It was found that ultrasonic frequency determines the time of periodic contact-separation between chisel edge and workpiece, and ultrasonic amplitude determines the maximum distance of contact-separation. Using ABAQUS finite element software, a polygon SiC particles model with a high-volume fraction was established to investigate the SiC particles crushing process under different ultrasonic-assisted milling conditions. The results showed that high-frequency ultrasonic-assisted milling could soften SiCp/Al composites, and the structural integrity of silicon carbide particles could be better maintained under appropriate ultrasonic amplitudes, reducing the probability of fragmentation. The removal mode was mainly plastic removal or crushing into small particles. The surface roughness value and milling force were reduced, improving the surface quality of the processed composite material. The conclusions of the milling test were basically consistent with the simulation results, which prove the correctness and feasibility of the simulation results

      • Comorbidity Relationship to Outcome of Radical Cystectomy in Chinese: a Single Institution Study with the ACE-27 Comorbidity Index

        Xuan, Zhu,Zhong, Zhao-Hui,Zhang, Xuan-Zhi,Zhang, Lei,Zhao, Xiao-Kun,Lv, Chen,Xu, Ran,Ren, Wei-Gang,Li, Song-Chao Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

        To determine the relationship between comorbidity and outcome after radical cystectomy in Chinese patients by using the Adult Comorbidity Evaluation (ACE)-27 index. Two-hundred-and-forty-six patients treated with radical cystectomy at the Second Xiangya Hospital of Central South University, Hunan Province, China between 2000 and 2010 were retrospectively analyzed. Medical records were reviewed for age, gender, delayed time of radical cystectomy, urinary diversion type, pelvic lymphadenectomy status, TNM stage, and pathological grade. Comorbidity information was assessed by the ACE-27 index. The outcome measurement was overall survival. Univariate and multivariate Cox proportional hazards regression analyses were used to determine the association between comorbidity and outcome. The study population consisted of 215 (87.40%) males and 31 (12.60%) females with a mean age of $62{\pm}11$ years. Median duration of follow-up was $47{\pm}31$ months. A total of 151 (61.38%) patents died during follow-up. Of those, 118 (47.97%) had at least one comorbidity. According to the ACE-27 scores, 128 (52.03%) patients had no comorbidity, 79 (32.11%) had mild, 33 (13.41%) had moderate, and 6 (2.45%) had severe comorbidities. Multivariate analysis indicated that moderate (p=0.002) and severe (p<0.001) comorbidity was significantly associated with decreased overall survival. In addition, age ${\geq}70$ years (p=0.002), delayed time of radical cystectomy >12 weeks (p=0.044), pelvic lymphadenectomy status (p=0.014), and TNM stage >T3 (p<0.001) were determined to be independent risk factors of overall survival. Increasing severity of comorbidity statistically correlated with decreased overall survival after radical cystectomy.

      • SCIESCOPUSKCI등재

        A regression for estimating metabolizable glucose in diets of weaned piglets for optimal growth performance

        Lv, Liangkang,Feng, Zhi,Zhang, Dandan,Lei, Long,Zhang, Hui,Liu, Zhengya,Ren, Ying,Zhao, Shengjun Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.10

        Objective: Two experiments were conducted to provide a new approach for evaluating feed nutritional value by metabolizable glucose (MG) in piglet diets with different levels of starch and crude fiber. In Exp 1, a regression equation for MG was generated. In Exp 2, the equation was verified, and the optimal growth performance of piglets under appropriate MG levels was tested. Methods: In Exp 1, 20 weaned piglets (7.74±0.81 kg body weight [BW]) were randomly assigned to 1 of 4 treatments, including the basal diet containing different levels of MG (starch, 25.80%, 31.67%, 45.71%, 49.36%; crude fiber, 1.23%, 1.35%, 1.80%, 1.51%). The piglets were implanted with an ileal fistula, cannulation of the carotid artery, portal vein, and mesenteric artery. The chyme from the ileum fistula and blood samples were collected. In Exp 2, 30 weaned piglets (8.96±0.50 kg BW) were randomly assigned to 1 of 5 treatments, including the experimental diets with different levels of MG (37.6, 132.5, 300.0, 354.3, and 412.5 g/kg). The piglets' BW, and feed consumption were recorded to calculate growth performance during the 28-d experiment. Results: In Exp 1, the MG levels in 4 diets were 239.62, 280.68, 400.79, and 454.35 g/kg. The regression equation for the MG levels and dietary nutrients was: Y (MG) = 12.13×X<sub>1</sub> (starch)+23.18×X<sub>2</sub> (crude fiber)-196.44 (R<sup>2</sup> = 0.9989, p = 0.033). In Exp 2, treatments with 132.5 and 300.0 g/kg MG significantly (p<0.05) increased average daily gain and feed conversion efficiency of weaned piglets, increased digestibility of crude fat, and had no effect on digestibility of crude protein compared to 300.0 to 412.5 g/kg MG. Conclusion: The pig model combining the ileum fistula and cannulation of blood vessels was successfully used to determine the dietary MG levels. The recommended MG level in weaned pig diets is 132.5 to 300.0 g/kg.

      • Human Papillomavirus Vaccine Awareness, Acceptability, and Decision-Making Factors among Chinese College Students

        Wang, Shao-Ming,Zhang, Shao-Kai,Pan, Xiong-Fei,Ren, Ze-Fang,Yang, Chun-Xia,Wang, Zeng-Zhen,Gao, Xiao-Hong,Li, Man,Zheng, Quan-Qing,Ma, Wei,Zhao, Fang-Hui,Qiao, You-Lin,Sivasubramaniam, Priya Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

        Background: College students are recommended as the target groups for catch-up human papillomavirus (HPV) vaccination. Systematical exploration of awareness, acceptability, and decision-making factors of HPV vaccination among Chinese college students has been limited. Materials and Methods: A multi-center survey was conducted in mainland China between November 2011 and May 2012. College students aged 18-22 years were stratified by their grade, gender, and major for sampling. Socio-demographic and HPV-related information such as knowledge, perceptions, acceptability, and attitudes were collected through a questionnaire. Results: A total of 3,497 undergraduates completed the questionnaire, among which 1,686 were males. The acceptability of the HPV vaccine was high (70.8%). Undergraduates from high-level universities, at lower grade, or with greater prior knowledge of HPV vaccines showed higher acceptability of HPV vaccination ($p_{trend}$ <0.001). Additionally, undergraduates with vaccination experience outside the National Expanded Program on Immunization (OR=1.29; 95%CI: 1.10-1.51) or fear of HPV-related diseases (OR=2.79; 95%CI: 2.28-3.41) were more willing to accept HPV vaccination. General knowledge of HPV vaccine was low among undergraduates, and safety was a major concern (71.05%). The majority of students wished to pay less than 300RMB for HPV vaccine and chose the Chinese Center for Disease Control and Prevention as the most appropriate venue for vaccination. Conclusions: Although most undergraduates demonstrate positive attitudes towards HPV vaccination, challenges pertaining to introduction exist in China. Corresponding proactive education and governmental subsidy to do so are urgently needed by this age-group population. Suggestions and potential strategies indicated may help shape the future HPV vaccination program in China.

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