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Tazarotene-Induced Gene 1 Interacts with DNAJC8 and Regulates Glycolysis in Cervical Cancer Cells
Chun-Hua Wang,Rong-Yaun Shyu,Chang-Chieh Wu,Mao-Liang Chen,Ming-Cheng Lee,Yi-Yin Lin,Lu-Kai Wang,Shun-Yuan Jiang,Fu-Ming Tsai 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.6
The tazarotene-induced gene 1 (TIG1) protein is a retinoid-inducible growth regulator and is considered a tumor suppressor. Here, we show that DnaJ heat shock protein family member C8 (DNAJC8) is a TIG1 target that regulates glycolysis. Ectopic DNAJC8 expression induced the translocation of pyruvate kinase M2 (PKM2) into the nucleus, subsequently inducing glucose transporter 1 (GLUT1) expression to promote glucose uptake. Silencing either DNAJC8 or PKM2 alleviated the upregulation of GLUT1 expression and glucose uptake induced by ectopic DNAJC8 expression. TIG1 interacted with DNAJC8 in the cytosol, and this interaction completely blocked DNAJC8-mediated PKM2 translocation and inhibited glucose uptake. Furthermore, increased glycose uptake was observed in cells in which TIG1 was silenced. In conclusion, TIG1 acts as a pivotal repressor of DNAJC8 to enhance glucose uptake by partially regulating PKM2 translocation.
Chunli Lu,Longbing Ma,Chenghua Yuan,Lei Cheng,Xinyu Wang,Wanru Duan,Kai Wang,Zan Chen,Hao Wu,Gao Zeng,Fengzeng Jian 대한척추신경외과학회 2022 Neurospine Vol.19 No.3
Objective: The specific association between morphometric characteristics of the syrinx and the prognosis of Chiari malformation type I (CM-I) with syringomyelia following surgical procedure seems to have not been fully elaborated. This study focused on the preoperative clinical and radiologic parameters in CM-I patients with syringomyelia to find out the relationship between the patients’ clinical status and the phenotypes of the syrinx with surgical outcome. Methods: A continuous series of pediatric and adult patients with CM-I and syringomyelia from a prospectively maintained database in a single center were included, and we explored the related factors affecting the prognosis following decompression surgery through retrospective analysis of clinical presentations, imaging characteristics, and the morphological features of syringomyelia, to provide a clinical reference for the treatment of syringomyelia. Results: There were 28 pediatric patients (13.8%), and 174 adults (86.2%) included in our study. The average Chicago Chiari Outcome Scale score was 14.56 ± 1.78. The overall prognosis after surgery was good in our series, among them 152 cases (75.25%) with a favorable prognosis, and syrinx was resolved effectively in 172 cases (85.15%). According to the univariate and multivariate analyses, the preoperative symptom duration, observation time, and with/without moniliform type were independent factors affecting the prognosis in adults. The most obvious difference between moniliform type and nonmoniliform type lies in the preoperative symptom duration, ventral subarachnoid space at the foramen magnum, and with/without straightened cervical physio-curve. Conclusion: Timely decompression surgery could achieve a better outcome in CM-I patients with syringomyelia. Moniliform syringomyelia may suggest a relatively better prognosis.
Wu, Songwei,Jian, Ming-Yuan,Xu, Yan-Chun,Zhou, Chun,Al-Mehdi, Abu-Bakr,Liedtke, Wolfgang,Shin, Hee-Sup,Townsley, Mary I American Physiological Society 2009 American Journal of Physiology: Lung cellular and Vol.297 No.4
<P>Pulmonary vascular endothelial cells express a variety of ion channels that mediate Ca(2+) influx in response to diverse environmental stimuli. However, it is not clear whether Ca(2+) influx from discrete ion channels is functionally coupled to specific outcomes. Thus we conducted a systematic study in mouse lung to address whether the alpha(1G) T-type Ca(2+) channel and the transient receptor potential channel TRPV4 have discrete functional roles in pulmonary capillary endothelium. We used real-time fluorescence imaging for endothelial cytosolic Ca(2+), immunohistochemistry to probe for surface expression of P-selectin, and the filtration coefficient to specifically measure lung endothelial permeability. We demonstrate that membrane depolarization via exposure of pulmonary vascular endothelium to a high-K(+) perfusate induces Ca(2+) entry into alveolar septal endothelial cells and exclusively leads to the surface expression of P-selectin. In contrast, Ca(2+) entry in septal endothelium evoked by the selective TRPV4 activator 4alpha-phorbol-12,13-didecanoate (4alpha-PDD) specifically increases lung endothelial permeability without effect on P-selectin expression. Pharmacological blockade or knockout of alpha(1G) abolishes depolarization-induced Ca(2+) entry and surface expression of P-selectin but does not prevent 4alpha-PDD-activated Ca(2+) entry and the resultant increase in permeability. Conversely, blockade or knockout of TRPV4 specifically abolishes 4alpha-PDD-activated Ca(2+) entry and the increase in permeability, while not impacting depolarization-induced Ca(2+) entry and surface expression of P-selectin. We conclude that in alveolar septal capillaries Ca(2+) entry through alpha(1G) and TRPV4 channels differentially and specifically regulates the transition of endothelial procoagulant phenotype and barrier integrity, respectively.</P>
Cubic time-spline fitting and interpolation for five-axis CNC machining
Wu Qingle,Yuan Chun-Ming,Shen Li-Yong,He Shi-Tao,Gao Xiao-Shan 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.6
In CNC machining, G01 codes are widely used to represent the tool path. Directly interpolating these G01 codes is time-consuming and may cause discontinuities. In this paper, we propose a time-spline curve fitting method that combines tool path fitting and feedrate scheduling into a single step for five-axis CNC machining. The input for this method consists of a three-dimensional linear path of the tool tip in the workpiece coordinate system and two-dimensional tool orientations in the machine coordinate system (MCS). The output is a fitted tool path in the MCS represented by a five-dimensional smooth time-parametric B-spline curve, simply referred to as the time-spline curve. The time-spline curve provides not only position information but also kinematic information, including velocity, acceleration, and jerk for each axis, directly derived from the first, second, and third derivatives of the curve. To meet fitting error constraints and axial kinematic constraints, our objective is to find the time-spline curve that is time-optimal. We formulate the optimization problem as a nonlinear optimization model and design a recursive algorithm to solve it. The resulting time-spline curve demonstrates high accuracy and fully utilizes the machine’s kinematic capabilities. Along the tool path defined by the time-spline curve, exact interpolation points can be straightforwardly obtained according to the interpolation period. Simulations and experimental results indicate that the proposed method yields a time-optimal time-spline curve with the desired precision and kinematic constraints.
( Yuan Zhao ),( Ke Pin Chen ),( Qing Yao ),( Yang Chun Wu ),( Jian Zhang ),( Xi Jie Guo ) 한국잠사학회 2006 International Journal of Industrial Entomology Vol.13 No.2
Seveval Chinese and Japanese varieties with good characters were used in the breeding. After 5 years (15 generations), a pair of robust and high quality silkworm variety with NPV resistance was bred by means of a combination of crossing and pedigree selection complemented by the selection of NPV resistance. The variety was identified jointly nationwide in 2003 and 2004, and appraised by National Mulberry and Silkworm Appraising Committee. Results are as follows: its cocooning rate is over 93%, shell rate 23-25%, filament length 1200-1300 meters, reelability 75-88%, Length of non-broken cocoon filament 900-1100 meters, raw silk rate 17-19%, neatness 95-97 points, and cocoon crop, cocoon shell weight and raw silk weight per 10 000 larvae is higher than those of the control variety by 7-10%, 14-19% and 14-18%, respectively. The variety is not only robust, resistant to high temperature and NPV, easy to rear, uniform in hatching, molting and maturing, but also lays more eggs, and its fecundity is high. It is suitable to rear in the Yangtze River Basin, the Yellow River basin and the Pearl River basin of China.
Microstructural Development of Brass Alloys with Various Bi and Pb Additions
Chih-Chun Hsieh,Jia-Siang Wang,Paxon Ti-Yuan Wu,Weite Wu 대한금속·재료학회 2013 METALS AND MATERIALS International Vol.19 No.6
In the study, using the gravity casting method, adding 1.52%Pb, 0.5%Bi, 1%Bi and 1.5%Bi into the brass (Cu- 40%Zn) alloy. The microstructural changes from the Widmanstätten into the networked structures when Pb was added to 1.5%. The microstructure was an acicular Widmanstätten when Bi contents were 0.5% and 1% and it was a plate Widmanstätten when Bi contents were 1.5%. There were four kinds of precipitation morphologies of Bi particles. The precipitation morphologies of Bi particles can be divided into a globular (<1 μm), a disc (=1 μm), discontinuous massive (>1 μm), and continuous block structures (about 20~30 μm). The Pb particles were embedded in the networked α phase and the Bi particles precipitated at the α/α and the α/β’ grain boundaries. The XRD analysis showed the high proportion of β’ phase with 0.5% Bi-brass and 1% Bi-brass and indicated a lower one with Pb-brass and 1.5% Bi-brass.
The Influence of Resilience on the Coping Strategies in Patients with Primary Brain Tumors
Shu-Yuan Liang,Hui-Chun Liu,Yu-Ying Lu,Shu-Fang Wu,Ching-Hui Chien,Shiow-Luan Tsay 한국간호과학회 2020 Asian Nursing Research Vol.14 No.1
Purpose: The purpose of this study was to assess the amount of variance in the coping strategies of patients with brain tumors that could be accounted for by resilience. Methods: This cross-sectional survey involved 95 patients who had experienced surgical, chemotherapy, or radiotherapy therapies for their brain tumors at least 1 month before data collection. The investigator collected data using the scales of the Ways of Coping Checklist-Revised and Resilience Scale. Data were analyzed using descriptive statistics, t tests, analysis of variance, Pearson product–moment correlation, and hierarchical multiple regression. Results: The results revealed that resilience was significantly positively associated with patients' problem-focused coping (r = .65, p < .001) and total coping (r = .49, p < .001). In addition, resilience accounted for 27% (R2inc = .27, p < .001) and 16% (R2inc = .16, p < .001) of the distinct variances in predicting patients’ problem-focused coping and total coping. Conclusion: The current results provide evidence to support the importance of resilience in shaping the coping strategies of relevant patients. As resilience shows a crucial element in patient coping with brain tumors, health team members should develop and employ appropriate strategies to improve the resilience of patients with brain tumors.
Tazarotene-Induced Gene 1 Interacts with DNAJC8 and Regulates Glycolysis in Cervical Cancer Cells
Wang, Chun-Hua,Shyu, Rong-Yaun,Wu, Chang-Chieh,Chen, Mao-Liang,Lee, Ming-Cheng,Lin, Yi-Yin,Wang, Lu-Kai,Jiang, Shun-Yuan,Tsai, Fu-Ming Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.6
The tazarotene-induced gene 1 (TIG1) protein is a retinoidinducible growth regulator and is considered a tumor suppressor. Here, we show that DnaJ heat shock protein family member C8 (DNAJC8) is a TIG1 target that regulates glycolysis. Ectopic DNAJC8 expression induced the translocation of pyruvate kinase M2 (PKM2) into the nucleus, subsequently inducing glucose transporter 1 (GLUT1) expression to promote glucose uptake. Silencing either DNAJC8 or PKM2 alleviated the upregulation of GLUT1 expression and glucose uptake induced by ectopic DNAJC8 expression. TIG1 interacted with DNAJC8 in the cytosol, and this interaction completely blocked DNAJC8-mediated PKM2 translocation and inhibited glucose uptake. Furthermore, increased glycose uptake was observed in cells in which TIG1 was silenced. In conclusion, TIG1 acts as a pivotal repressor of DNAJC8 to enhance glucose uptake by partially regulating PKM2 translocation.