Regulatory Mechanisms of Annexin-Induced Chemotherapy Resistance in Cisplatin Resistant Lung Adenocarcinoma

Wang, Chao,Xiao, Qian,Li, Yu-Wen,Zhao, Chao,Jia, Na,Li, Rui-Li,Cao, Shan-Shan,Cui, Jia,Wang, Lu,Wu, Yin,Wen, Ai-Dong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Adenocarcinoma of lung has high incidence and a poor prognosis, woith chemotherapy as the main therapeutic tool, most commonly with cisplatin. However, chemotherapy resistance develops in the majority of patients during clinic treatment. Mechanisms of resistance are complex and still unclear. Although annexin play important roles in various tumor resistance mechanisms, their actions in cisplatin-resistant lung adenocarcinoma remain unclear. Preliminary studies by our group found that in cisplatin-resistant lung cancer A549 cells and lung adenocarcinoma tissues, both mRNA and protein expression of annexins A1, A2 and A3 is increased. Using a library of annexin A1, A2 and A3 targeting combined molecules already established by ourselves we found that specific targeting decreased cisplatin-resistance. Taken together, the underlined effects of annexins A1, A2 and A3 on drug resistance and suggest molecular mechanisms in cisplatin-resistant A549 cells both in vivo and in vitro. Furthermore, the study points to improved research on occurrence and development of lung adenocarcinoma, with provision of effective targets and programmes for lung adenocarcinoma therapy in the clinic.

카드뮴에 의해 유도된 담배 식물의 생장, 엽록소와 rubisco/rubisco activase에 대한 salicylic acid의 전환 효과

Yu Shan Wang(王玉珊),Kwang Soo Roh(노광수) 한국생명과학회 2012 생명과학회지 Vol.22 No.6

카드뮴에 의해 유도되는 담배의 생장, 엽록소 함량, rubisco와 rubisco activase에 미치는 SA의 영향과, 이에 대한 변성제의 효과를 연구하였다. 담배 기내생장에 대한 SA의 최적농도를 찾기 위해, 10?? mM - 10² mM SA를 처리하여 9주간 생장시킨 결과, 10?⁴ mM SA에서 가장 높은 생장을 보였다. SA와 카드뮴을 4개의 실험구(대조구, SA, 카드뮴, 카드뮴 + SA)로 하여 생장, 엽록소 함량 및 rubisco와 rubisco activase의 함량과 활성을 측정한 결과, 카드뮴 > 카드뮴 + SA < 대조구 < SA 순서이었으며, 대조구보다 카드뮴을 처리했을 때 낮았고, 대조구보다 SA에서 높았으며, 카드뮴 보다 SA에서 높게 나타났다. 이는 카드뮴에 의해 유발된 억제 현상이 SA에 의해 반전되었음을 의미하는 것으로, SA는 담배 식물의 기내생장, 엽록소 및 rubisco/rubisco activase에 대한 카드뮴의 손실 효과를 개선시키는 positive effect 작용을 하는 것으로 나타났다. Rubisco의 활성에 대한 변성제 들은 카드뮴 > 카드뮴 + SA < 대조구 < SA 순서로 효과를 나타냈으며, 이 중 L-cysteine과 β-mercaptoethanol은 활성을 촉진시켰으며, urea, thiourea, guanidium-HCl은 억제시켰다. 이는 L-cysteine과 β-mercaptoethanol은 변성에 관여하지 않으며, urea, thiourea, guanidium-HCl은 변성에 관여하였음을 의미한다. Rubisco activase의 활성에 대한 변성제들의 영향을 조사한 결과, 5종 모두는 비처리구와 비교하여 현저한 영향은 나타내지 않았다. The influence of salicylic acid (SA) on growth, chlorophyll, and rubisco/rubisco activase and effect of denaturator on rubisco/rubisco activase activity were studied in tobacco plants grown in vitro with cadmium (Cd) treatment. In order to find out the optimum concentration of SA, tobacco plants treated with 10?? mM?10² mM of SA were grown in MS medium for 9 weeks, respectively. The most pronounced effect on in vitro growth was found at 10?⁴ mM SA. Among the control (not treated with Cd and SA), SA, Cd, and Cd + SA, the growth and content of chlorophyll were in the sequence of Cd < Cd + SA < control < SA, and significantly higher at SA compared with others. Similar results were also observed in the content and activity of rubisco and rubisco activase. These data suggest that inhibitory effect by Cd was reversed by SA. These results also indicate that SA has a positive effect on Cd. The effect of denaturants on rubisco activity showed in the sequence of Cd < Cd + SA < control < SA. Rubisco activity was promoted by L-cysteine and β-mercaptoethanol, not by urea, thiourea, and guanidium-HCl. These data suggest that urea, thiourea, and guanidium-HCl are able to act as denaturator, and L-cysteine and β-mercaptoethanol are not. None of the five denaturants affected the activity of rubisco activase.

Analysis Of the Total Electrical Length Extremum Of TSSIR Using Lagrange Multipliers Method

Wang Shan,Sun Yu,Wang Yao,Yu Yiqiang 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.1

A systematic approach to determine the extreme value of total electrical length for tri-section stepped impedance resonator (TSSIR) is proposed in this paper. It utilizes the Lagrange multipliers method to search the extremum condition. Result demonstrates that the maximum resonator length of TSSIR is limited to triple that of the corresponding uniform impedance resonator (UIR). Spurious resonant frequency of λ/2 TSSIR is mainly analysized under the extremum condition (θ1≠θ2≠θ3). Through comparison between the proposed approach and previously assumed condition (θ1=θ2=θ3), the staircase discontinuity of transmission line can be reduced using the paper theory to achieve same harmonic ratio. Last, a simulated example is presented to verify correctness of the method. This paper aims to provide a theoretical basis and general guideline for the filter design using TSSIR.

Sensitivity of Plasmodium falciparum to Antimalarial Drugs in Hainan Island, China

Shan-Qing Wang,Guang-Ze Wang,Yu-Chun Li,Feng Meng,Shi-Gan Lin,Zhen-Hu Zhu,Ding-Wei Sun,Chang-Hua He,Xi-Min Hu,Jian-Wei Du 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1

Effects of Rad51 on Survival of A549 Cells

Yu, Sha-Sha,Tu, Yi,Xu, Lin-Lin,Tao, Xue-Qin,Xu, Shan,Wang, Shan-Shan,Xiong, Yi-Feng,Mei, Jin-Hong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1

Rad51, a key factor in the homologous recombination pathway for the DNA double-strand break repair, plays a vital role in genesis of non-small-cell lung cancer (NSCLC). In recent years, more and more studies indicate that high expression of Rad51 is of great relevance to resistance of NSCLC to chemotherapeutic agents and ionizing radiation. However, the underlying molecular mechanisms are poorly understood. In this study, we investigated the role of single Rad51 on cell viability in vitro. Our results show that depletion of endogenous Rad51 is sufficient to inhibit the growth of the A549 lung cancer cell line, by accumulating cells in G1 phase and inducing cell death. We conclude that independent Rad51 expression is critical to the survival of A549 cells and can be an independent prognostic factor in NSCLC patients.

Protective Effect of Schisandra chinensis Polysaccharides Against the Immunological Liver Injury in Mice Based on Nrf2/ARE and TLR4/NF-κB Signaling Pathway

Yingying Shan,Bin Jiang,Jiahui Yu,Jiaye Wang,Xiaoli Wang,He Li,Chunmei Wang,JianGuang Chen,Jinghui Sun 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.9

We have previously demonstrated the hepatoprotective effect of Schisandra chinensis polysaccharides (SCP) against the liver injury induced by alcohol, high-fat diet, and carbon tetrachloride in mice. In this study, we investigated the effect of SCP against the immunological liver injury induced by concanavalin A (Con A) in mice. The results showed that SCP could significantly reduce the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the serum of mice with immunological liver injury. SCP could significantly decrease the content of malondialdehyde (MDA) and nitric oxide (NO) and increase the activity of superoxide dismutase (SOD) and glutathione (GSH) in the liver tissue. SCP could significantly increase the number of CD4+ and decrease the number of CD8+ in the peripheral blood, and elevate the ratio of CD4+/CD8+. SCP could significantly downregulate the expression of Kelch-like ECH-associated protein 1 (Keap1) and upregulate the expression of nuclear factor-erythroid 2-related factor2 (Nrf2) and downstream gene heme oxygenase-1 (HO-1), and downregulate the expression of toll-like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (MyD88), and nuclear factor-kappa B (NF-κB) proteins. This study indicates that SCP can reduce the release of a large number of inflammatory factors to inhibit the oxidative stress in mice with the immunological liver injury induced by Con A, and its mechanism is closely related to the regulation of Nrf2/antioxidant response element and TLR4/NF-κB signaling pathways.

Influence of Rashba Spin-orbit Coupling on Fano-Kondo Effect in a Parallel Double-quantum-dot Structure

Jing Shan,Yun-Gang Wang,Yu Wang,Hui-Min Wang,Yu Han 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.64 No.3

Electron transport through a parallel double-quantum-dot structure is theoretically studied withone dot in the Kondo region and the other one in the region of local Rashba interaction. Thecoupling between the Kondo dot and the electron reservoirs is found to offer a reference channel forelectron travels, and the connection of the Rashba dot and the leads is found to afford a resonantchannel. The interplay of these two mechanisms gives rise to the Fano effect, which also depends onthe adjustment of the Rashba field. We then conclude that in this structure, the Fano interferenceis more robust because it causes a quenching of the Kondo resonance. In the presence of a localmagnetic flux, the quenched Kondo resonance becomes determined by the electron spin, which ishelpful for spin manipulation.

Prevalence and Age, Gender and Geographical Area Distribution of Esophageal Squamous Cell Carcinomas in North China from 1985 to 2006

Feng, Xiao-Shan,Yang, Yan-Tong,Gao, She-Gan,Ru, Yi,Wang, Gong-Ping,Zhou, Bo,Wang, Yu-Feng,Zhang, Peng-Fei,Li, Pu-Yu,Liu, Yong-Xuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Objective: To establish the prevalence and distribution profile of esophageal squamous cell carcinomas (ESCCs) over a 22-yr period in North China. Methods: Using endoscopy for primary diagnosis and histological analysis for the further confirmation, a total of 74,854 ESCC patients aged 20-89 between January 1985 and December 2006 were investigated to analyze the epidemiological profile including prevalence rates, distribution of age-of-onset, gender and geographical area of ESCC in Luoyang, the highest incidence area of North China. Results: A total of 4092 cases of ESCC were finally diagnosed among 74,854 patients who had their first endoscopies. The prevalence among males was higher than that among females (p<0.01), resulting in an overall male:female OR of 1.2 (95%CI, 1.2-1.3). The prevalence in rural areas was higher than in urban areas (p<0.01), resulting in an overall rural:urban OR of 2.6 (95%CI, 2.4-2.9). The rural:urban ORs and the 95% CI increased continuously from 2.6, 2.3-3.0 to 2.7, 2.2-3.3, respectively, for 4 consecutive periods during the 22-yr study period. Moreover, the median age of onset among females was higher than that among males (p<0.01). For both sexes and in both areas, the prevalence rates declined and the median age of onset rose for 4 consecutive periods in the 22-yrs time frame (p<0.01). Conculsions: These data reveal the epidemiological profile of ESCC in the area of North China, and suggest that urban areas and rural people account for a growing proportion of the ESCC patients although the prevalence of ESCC significantly declined and the median age-of-onset postponed over the 22-yrs period. Moreover, the prevalence status of ESCC in rural areas also underlines the need for public health initiatives aimed at reducing risk factors of this fatal disease.

South Korean Translations of Chinese Literary

Jin Chang-Shan, Wang-Yu 조선대학교 국제문화연구원 2014 국제문화연구 Vol.7 No.1

South Korean translations of Chinese literary works in the early 1980s had been totally different from that in the late 1980s. In the firse half period, the South Korea had been do minated by the new military which had censored and regulated the right to free speech and press. Therefore, its translations of Chinese literary works in this period can be regarded as nothing but the continuation of that in the 1970s, with only translations of Chinese classic literary works and martial arts novels as well as works by Lu Xun, Lin Yutang. Such situation had not been improved until the second half of the 1970s when works by writers in socialist countries had been allowed to be introduced into South Korea. Until the second half of the 1980s, the modern Chinese literary works had the opportunity to be read by readers in SouthKorea. In the second half of the 1980s especially after the “6·29 Democratic Declaration” in 1987, literary works by writers in socialist countries like China had been publicly and officially allowed to be translated in South Korea. Some completed works collecting many classic literary works about Chinese socialism, such as Modern Chinese Collected Works, have been colored strongly with socialism and exerted powerful impacts. And allowing the translation of such completed works by the South Korean government shows that its thirty-year’s bans on the introduction of socialist literary works had been thoroughly lifted, which was a milestone in its history of ranslatingliteraryworks.

Parkinson’s Disease with Fatigue: Clinical Characteristics and Potential Mechanisms Relevant to α-Synuclein Oligomer

Li-Jun Zuo,Shu-Yang Yu,Fang Wang,Yanghui Xia,Ying-Shan Piao,Yang Du,Teng-Hong Lian,Rui-Dan Wang,Qiu-Jin Yu,Ya-Jie Wang,Xiao-Min Wang,Piu Chan,Sheng-Di Chen,Yongjun Wang,Wei Zhang 대한신경과학회 2016 Journal of Clinical Neurology Vol.12 No.2

Background and Purpose The aim of this study was to identify the clinical characteristics and potential mechanisms relevant to pathological proteins in Parkinson’s disease (PD) patients who experience fatigue. Methods PD patients (n=102) were evaluated using a fatigue severity scale and scales for motor and nonmotor symptoms. The levels of three pathological proteins—α-synuclein oligomer, β-amyloid (Aβ)1-42, and tau—were measured in 102 cerebrospinal fluid (CSF) samples from these PD patients. Linear regression analyses were performed between fatigue score and the CSF levels of the above-listed pathological proteins in PD patients. Results The frequency of fatigue in the PD patients was 62.75%. The fatigue group had worse motor symptoms and anxiety, depression, and autonomic dysfunction. The CSF level of α-synuclein oligomer was higher and that of Aβ1-42 was lower in the fatigue group than in the non-fatigue group. In multiple linear regression analyses, fatigue severity was significantly and positively correlated with the α-synuclein oligomer level in the CSF of PD patients, after adjusting for confounders. Conclusions PD patients experience a high frequency of fatigue. PD patients with fatigue have worse motor and part nonmotor symptoms. Fatigue in PD patients is associated with an increased α-synuclein oligomer level in the CSF