退溪의 政治道義 實現과 聖君의 役割 : 聖學十圖를 중심으로 Center on Sunghaksipdo(『聖謔十圖』)

柳星烈 단국대학교 퇴계학연구소 1996 退溪學硏究 Vol.10 No.-

DDC,MEA,WR-2721의 放射線 防護效果에 關한 硏究

柳星烈,陳秀一,鄭鉉佑,鄭引溶,高柱煥,고경환 대한방사선 방어학회 1986 방사선방어학회지 Vol.11 No.2

放射線障害 豫防藥劑의 人體適用은 副作用으로 臨床的 利用이 되지 못한 實情으로서 生理學的 및 藥理學的 毒性, 物理學的 諸性質, 藥劑의 作用機轉究明 및 臨床的 技術開發이 未洽하여 放射線 障害者에 對한 治療는 對症療法에 不過한 實情이다. 著者들은 放射線에 依한 人體障害豫防에 必要한 基礎資料를 얻기 위하여 現在까지 比較的 毒性이 적고 化學的으로 安定하며 藥效가 優秀하다고 알려진 DDC, MEA, WR-2721을 選定하여 實驗動物 非近交系 Swiss Webster(NIH-GP)마우스에 利用한 放射線 防護作用에 關한 實驗結果는 다음과 같다. 1) 各種 放射線 防護劑(DDC, MEA, WR-2721)의 마우스에 대한 適定 投與量은 各各 DDC; 1,000, MEA; 200, WR-2721; 400mg/kg이었다. 2) 實驗動物에 放射線 反致死線量 LD50/10에 對한 藥劑의 防護效果을SMS DDC;1.2, MEA; 1.4, WR-2721; 1.9이었고 LD50/30에 對 한 DMF는 DDC; 1.7, MEA; 1.8, WR-2721; 2.5이었다. 3) 空腹 小?腺(Jejunum crypt)의 放射線平均致死線量(Do)에 對한 防護效果 DMF는 DCC; 1.07, MEA; 1.21, WR-2721; 1.76이었고, 小?腺細胞의 致死線 平均致死線量에 對한 DMF는 DDC; 1.04, MEA; 1.08, WR-2721; 1.38이었다. 4) 以上으로 上記 3種의 放射線 防護劑中 WR-2721의 放射線 防護效果가 가장 優秀하였고 이 結果는 向後 臨床的 適用에 關한 硏究의 基礎資料가 될 것으로 思料된다. At present, the treatments of the radiation-induced diseases are only performing by the palliative treatment technique. Moreover, radiation protective agents are a little toxic for human being and this seriously limits their applicability with various complications in clinical uses. Accordingly, as a part of the aim of gain of the basic data for protective roles of some radioprotectors, the present investingation was carried out to evaluate the comparative radioprotective effects by the administration of DDC, MEA, WR-2721. Results are shown for statistically significant analysis and correlation with each group as follows; 1. The proper doses of the radioprotectors were DDC; 1,550mg/kg, MEA; 450mg/kg, WR-2721; 780mg/kg of the mouse body weight. 2. DMF(Dose modification factor) of LD50/10 and LD50/30 for whole body irradiation was DDC; 1.2, MEA; 1.4, WR-2721; 1.9 and DDC; 1.7, MEA; 1.8 and WR-2721; 2.5 respectively. 3. DMF for radiation reaction of jejunal crypt was DDC; 1.07, MEA; 1.21 and WR-2721; 1.76 and that of jejunal crypt cell was DDC; 1.04, MEA; 1.08 and WR-2721; 1.38 respectively. 4. Conclusively, WR-2721 was the most effective drung among the three radioprotectors and this result must be a supportive data for further study for clinical application.

우라늄오염에 의한 신부전증에 미치는 제염제의 방호효과

김태환,정인용,김성호,김경중,방효창,류성렬,진수일 대한방사선 방어학회 1990 방사선방어학회지 Vol.15 No.2

원자력산업의 시설증대로 우라늄 오염의 가능성이 증가되고 있음에도 불구하고 종사자 및 국민에대한 안전대책 및 의료적 처치에 관한 연구가 매우 미흡한 실정에 있어 이에 대한 응급처치방안을 수립코자 우라늄을 투여한 후 제염제를 투여하여 관찰하였던 바 다음과 같은 결론을 얻었다. 1. 우라늄오염에 의한 체중변화에 미치는 제염제의 영향에 있어서 sodium bicarbonate와 생리적 식염수를 병행투여한 군과 우라늄을 투여하고 30분이 지나서 dithiothreitol을 투여한 군에서는 우라늄단독투여군에 비해 체중이 현저하게 증가하였다. (P<0.05). 2. 모든 실험군은 우라늄오염에 의한 음수량과 배뇨량의 변화를 유의성 있게 호전시켰으며(P<0.05), 특히 sodium bicarbonate와 생리적 식염수를 병행투여한 군과 우라늄을 투여하고 30분이 지나서 dithiothreitol을 투여한 군이 가장 높은 증가경향을 나타냈다(P<0.05). 3. 우라늄오염에 의한 BUN농도 변화에 미치는 제염제의 효과는 sodium bicarbonate와 생리적 식염수를 병행투여한 군과 우라늄을 투여하고 30분이 지나서 dithiothreitol를 투여한 군이 공히 우라늄단독투여군보다 BUN농도가 매우 감소되었다(P<0.01). 4. 우라늄을 투여하고 30분이 지나서 dithiothreitol를 투여한 군은 우라늄오염에 의한 serum creatinine의 농도증가를 유의하게 감소시켰으나 (P<0.01), sodium bicarbonate와 생리적 식염수를 병행투여한 군은 다소 감소하는 경향으로 나타났다. 5. 우라늄오염에 의한 urine creatinine농도 변화에 미치는 제염제의 효과에 있어서 sodium bicarbonate와 생리적 식염수를 병행토여한 군과 우라늄을 투여하고 30분이 지나서 dithiothreitol를 투여한 군에서는 우라늄단독투여군에 비해 creatinine의 배설이 상당히 증가하였다(P<0.05). 6. 우라늄오염에 의한 신장의 소견에 있어 우라늄단독투여군은 근위곡세뇨관상피의 공포화 및 종창, microvilli와 brush border의 손실, 세뇨관 상피의 괴사가 관찰되었으며, 간장의 충혈, 중심성 괴사 우라늄을 투여하고 30분이 지나서 dithiothreitol를 투여한 군에서는 우라늄단독투여군에 비해 높은 방호효과가 관찰되었으나 다른 실험군에서는 큰 효과가 없는 것으로 나타났다. 결론적으로 우라늄의 체내오염시에는 sodium bicarbonate와 생리적 식염수를 가능한 빨리 병행투여하거나 dithiothreitol을 체내오염후 30분이 지나서 투여하는 방법이 우라늄오염에 대한 제염에 매우 유효할 것으로 생각되며, 특히 우라늄에 의한 인체장해를 유의하게 경감시켜줄 것으로 사료되었다. Appreciable radiation exposures certainly were occurred m the reactor burn-up, the nuelear fall-out and the surroundings of nuclear installations with radioactive effluents. Therefore ,radioactive nuclides is not only potentially hazardous to workers of nuclear power plants and related industrials, but also the wokers who handle radioactive nuclides in biochemical research and nuclear medicine diagnostics. And in the case of occurring the nuclear accidents, the early medical treatment of radiation injury should be necessary but little is established'medical procedures to decontaminate the victims of internal contamination of radioactive nuclides in korea. Accordingly, to achieve the basic data for protective roles and medical treatment of radiation injury, the present studies were can-id out to evaluate the decontamination of uranium by the chemical drugs. The results observed were summarized as follows : 1. The combined treatmet group of sodium bicarbonate and saline with uranyl nitrate injection simultaneously and the dithiothreitol group that was administered 30 minutes after uranyl nitrate injection were increased significantly in the change of body weight than uranyl nitrate-only group(P<0.005). 2. All the experimental groups were increased the fluid intake and urine volume on the uranyl nitrate-induced acute renal failure, but the combined treatment group of sodium bicarbonate and saline with uranyl nitrate injection simultaneously and the dithiothreitol group that was administered 30 minutes after uranyl nitrate injection have the higher increment of fluid intake and urine volume(P<0.05). 3. When sodium bicarbonate and saline was treated with uranyl nitrate injection simultaneously, and dithiothreitol was administered 30 minutes after uranyl nitrate injection, there was significantly reduced in BUN concentration (P<0.0l). 4. When dithiothreitol was administered 30 minutes after uranyl nitrate injection, there was reduced more significantly on the increment of serum creatinine concentration than that observed in uranyl nitrate-only group(P<O.Ol). but when the combined treatment of sodium bicarbonate and saline with uranyl nitrate simultaneously, there was still, albeit much less marked, decrease in serum creatinine concentration. 5. The sodium bicarbonate and saline was treated with uranyl nitrate simultaneously and dithiothreitol was administered at 30 minutes after uranyl nitrate were excreted markedly higher urine creatinine concentration than the uranyl nitrate-only group. 6. Uranyl nitrate has been used in experimental animals to produce hydropic degeneration and swelling of proximal tubules, disappearance of microvilli and brush border or necrosis in the kidney and centrilobular necrosis, congestion, and telangiectasia of the liver. When the sodium bicarbonate and saline was treated with uranyl nitrate simultaneously, and dithiothreitol was administered 30 minutes after uranyl nitrate, there was more marked the protective effect than uranyl nitrate-only group. Finally, if the sodium bicarbonate and saline may administered as quickly as possible each time that some risk for internal contamination with uranium, and dithiothreitol is administered 30 minutes after uranium contamination, there ameliorates the course of uranyl nitrate-induced acute renal failure,and this effect is assocciated with prevention of uranium (heavy metal) induced alterations in BUN. serum creatinine. urine creatinine. fluid intake, urine volume and body weight.

KCCH cyclotron neutron 및 60Co r-ray에 의한 인체 말초혈액 임파구의 염색체 이상측정

김성호,김태환,정인용,조철구,고경환,류성렬 대한방사선 방어학회 1992 방사선방어학회지 Vol.17 No.1

KCCH cyclotron neutron(30cCy/min) 및 ??Co γ-ray(210cGy/min)를 시험관내의 정상 인체 말초혈액임파구에 조사하여 염색체이상(dicentric 및 centric ring)을 관찰하고 이의 선량-반응관계식을 linear model(Y=K₁D+a), power-law model(Y=K₂Dⁿ), quadratic model(Y=K₃D²) 및 linear-quadratic model(Y=αD+βD²)을 사용하여 구하고 이들 model중 염색체이상의 측정치와 가장 일치하는 관계식을 근거로 하여 γ -ray에 대한 neutron의 relative biological effectiveness(RBE)를 산출하였다. 염색체 이상(dicentric plus centric ring)의 발생분포는 γ-ray의 경우 linear model(P=0.067)을 제외한 power-law model[Y=(5.81±1.96)×10??, P=0.931], quadratic model [Y=(3.91±0.09)×10?D², P=0.972] 및 linear-quadratic model [Y=(6.55±6.83)×10?D+(3.72±0.22)×10?D², P=0.922]에 적합하였다. Neutron의 경우 linear model [Y=(6.12±0.17)×10?³ D-022, P=0.987]에 가장 일치하였고 quadratic model (P<0.005)을 제외한 power-law model[Y=(2.43±0.70)×10?³D+(1.21±0.39)×10?D², P=0.415]에 비교적 적합하였다. 세포당 0.1-1.5개의 염색체이상을 나타내는 neutron의 γ-ray에 대한 RBE는 2.714±0.408이었다. The frequencies of KCCH cyclotron neutron(30cCy/min) or ??Co γ-ray(210cGy/min)-induced asymmetrical interchanges(dicentrics and centric ring) and acentric fragment(deletion) at several doses were measured in the normal human peripheral blood lymphocytes. Chromosome aberrations were scored at the first nitosis after stimulation with phytohemagglutinin. The neutron and γ-ray data were analysed on liner, power-law, quadratic and linear-quadratic model. When the dicentrics and centric rings of γ-ray datas were pooled and fitted to these model, good fits were obtained to power-law [Y=(5.81±1.96)×10??, P=0.931], quadratic [Y=(3.91±0.09)×10?D², P=0.972] 및 linear-quadratic model [Y=(6.55±6.83)×10?D+(3.72±0.22)×10?D², P=0.922] except for linear model(P=0.067). As in the case of neutron data, the best fits was obtained to the linear model [Y=(6.12±0.17)×10?³ D-022, P=0.987] and good fits were obtained to power-law[Y=(5.36±3.02)×10?⁴D??, P=0.601] and linear-quadratic model [Y=(2.43±0.70)×10?³D+(1.21±0.39)×10?D², P=0.415], except for quadratic model (P<0.005). The relative biological effectiveness(RBE) of neutron compared with γ-ray was estimated by best fitting model. In the asymmetrical interchanges range between 0.1 and 1.5 per cell, the REE was found to be 2.714±0.408.

An Experimental Study of Radioprotective Effect of Ginseng Alkaloid Fraction on Cellular Damage

Seong Yul Yoo,Chul Koo Cho,Mi Sook Kim,Hyung Jun Yoo,Seong Ho Kim,Tae Hwan Kim 대한방사선방어학회 1997 방사선방어학회지 Vol.22 No.3

An Experimental Study of Radioprotective Effect of Ginseng Alkaloid Fraction on Cellular Damage

Yoo,Seong-Yul,Cho,Chul-Koo,Kim,Mi-Sook,Yoo,Hyung-Jun,Kim,Seong-Ho,Kim,Tae-Hwan 대한방사선 방어학회 1997 방사선방어학회지 Vol.22 No.3

본 연구에서는 인삼의 알카로이드 분획 (alkaloid fraction)을 주요성분으로 함유하는 Adaptagen의 방사선 방어제로서의 효과를 구명(究明)하기 위하여, 방사선조사에 의한 마우스 공장 소낭선 세포 손상 및 회복을 측정하고, 마우스 비장임파구 미세핵 측정법을 시험관 내 및 생체에서 실시함으로써 본 제재의 방사선 방어효과를 평가하였으며, 실험결과 얻어진 결론은 다음과 같다. 1. 마우스 공장소낭선세포의 방사선 손상은 대조군에 비해 alkaloid 분획 투여시와 수용성 분획 투여시 공히 감소하였으나, alkaloid 분획 투여시 더욱 현저하였다. 2. 마우스 공장소낭선세포 손상의 회복 및 증식은 대조군에 비해 alkaloid 분획 투여시와 수용성 분획 투여시 공히 현저히 증가하였다. 3. 시험관내 시험에서 방사선에 의한 임파구 미세핵 형성도는 쌍핵세포의 형성율이 낮아 통계학적 의의는 없으나 약제 투여군에서 빈도가 낮은 경향을 보였으며 alkaloid 분획 투여시 더욱 현저하였다. 4. 생체내 시험에서 방사선에 의한 임파구 미세핵 형성빈도는 대조군에 비해 alkaloid 분획 투여시와 수용성 분획 투여시 공히 감소하였으나 alkaloid 분획 투여시 더욱 현저하였다. 이상의 결과에서 인삼의 alkaloid 및 수용성 분획이 방사선에 의한 염색체 손상을 억제하고 손상된 세포의 회복 (repair) 및 증식 (regeneration)을 촉진하여 방사선 방어효과를 나타냄을 알 수 있엇으며, 이는 독성이 비교적 없는 자연산생물 (natural products)로서 방사선방어제로 임상에 직접 사용 할 수 있다. This paper is to assess the effect of Adaptagen as a radioprotector in which main component is alkaloid fraction of ginseng. Evaluation was made in vitro and in vivo study with NIGP)S) mouse by the measurement of regeneration of jejunal crypt cell and micronucleus assay to analyze radioprotective effect of ginseng alkaloid fraction in comparison with that of water fraction after whole body irradiation. The results were as follows, 1. The degree of radiation damage of mouse jejunal crypt cell was diminished in both of alkaloid and water fraction groups compared to control group but more in alkaloid fraction group than water fraction group. 2. Regeneration of mouse jejunal crypt cell was higher both in alkaloid and water fraction groups than control group. 3. In vitro study, frequency of micronucleus was diminished in tendency for the treated groups than control group but statistically insignificant. 4. In vitro study, frequency of micronucleus was diminished in both alkaloid and water fraction groups compared to control group but more in alkaloid fraction group than water fraction group.

Compositional Qualification of Radiation Protection in Neutron Radiotherapy Room with KCCH Cyclotron

Yoo,Seong Yul,Cho,Chul Koo,Koh,Kyoung Hwan 대한방사선 방어학회 1989 방사선방어학회지 Vol.14 No.2

원자력병원 싸이클로트론 中性子線 치료실의 방사선 準位를 측정함으로써 방사선 안전도를 검토하여 보았다. 중성자선 치료실내 방사선 노풀은 주로 isocentric gantry에 내장된 중성자선 標的과 照射野를 결정하는 collimator의 放射化로 인한 殘留放射能(remnent radioactivity)에 의하여 결정된다. 측정결과 線量率은 과다하지 않았고 개인 集積線量도 허용치 이내였다. 방사선ㄴ 작업종사자로서의 의료기사는 환자치료시 매 照射 완료 직후부터 5분간 減殺시간을 갖도록 조치하였다. Radiation exposure of the personnel in the neutron therapy facility of KCCH cyclotron neutron system is discussed. In neutron therapy room, medical personnel is exposed to photons of the remanent induced radioactivity from the isocentric gantry in which targets and collimators arc mounted. The radiation level of the neutron therapy room of KCCH cyclotron was acceptable and it decreased immediately after beam off. Personal exposure measured by individual monitor was far less than permissible level.

Compositional Qualification of Radiation Protection in Neutron Radiotherapy Room with KCCH Cyclotron

Seong Yul Yoo,Chul Koo Cho,Kyoung Hwan Koh 대한방사선방어학회 1989 방사선방어학회지 Vol.14 No.2

수술불능 및 재발성 직장암에 대한 중성자선 치료

류성렬 (Seong Yul Yoo),고경환 (Kyoung Hwan Koh),조철구 (Chul Koo Cho),박우윤 (Woo Yun Park),윤형근 (Hyong Geun Yun),심재원 (Jae Won Shim) 대한방사선종양학회 1993 Radiation Oncology Journal Vol.11 No.1

Total of 53 patients of unresectable and recurrent rectal cancer treated with neutron beam during the period from Oct. 1987 to Apr. 1992 were analyzed. Dose fractionation for the neutron only group was 1.5 Gy per fraction, 3 fraction per week, 21 Gy/4¹/₂ wks for 42 patients out of 53 (76%). Neutron only but modified fractionation schedule (10% more or less of total dose) was applied for 9 patients, and mixed beam (neutron boost) was for 4 patients. Complete tumor response was obtained in 40 patients (76% response rate). Local control rate was 28 out of 53 (53%). Statistically significant better prognostic factors for local control were age below 49 years old (15/22, 68%) than above 50 years old (13.31, 42%), male (20/32, 63%) than female (8/21, 38%), tumor size less than 5 cm and non-metastatic (16/24, 67%) than size more than 5 cm or metastatic (12/29, 41%). Major complication had developed in 7 patients (13%). Two year overall survival rate by Kaplan-Meier method was 30%, but it was rised to, 47% when the tumor was less than 5 cm non-metastatic.

두경부암의 근접방사선 치료

류성렬(Seong Yul Yoo) 대한두경부종양학회 1991 대한두경부 종양학회지 Vol.7 No.1

Brachytherapy is a method of radiotherapy in advantage to achieve better local control with minimum radiation toxicity in comparison with external irradiation because radiation dose is distributed according to the inverse square low of gamma-ray emitted from the implanted sources. The main characteristics of brachytherapy are delivering of higher dose to target volume shortening of total treatment period and sparing of normal tissue. Recent development of iridium ribbons for low dose rate implant provides improvement of technology of brachytherapy in terms of safety and efficiency. High dose rate method. on the other hand, is effective to avoid unnecessary expoure of medical personnel.