Apoptotic Effects of Sipimikwanjung-tang of Sasang Constitutional Medicine in Human Hepatoblastoma Cells

Song Seung-Yun,Bae Young-Chun,Lee Sang-Min,Kim Kyung-Yo,Joo Jong-Cheon,Ko Ki-Duk,Park Soo-Jeong,Lee Kyung-Sung,Choi Yong-Seok,Kim Jong-Yeol The Society of Korean Medicine 2005 대한한의학회지 Vol.26 No.1

Objective : This study on Sipimikwanjung-tang was undertaken to evaluate its antioxidant capacities and antiperoxidation activities in rat liver tissues. Sipimikwanjung-tang which has been one of the prescriptions in sasang constitutional medicine is usually applied for the therapy of various liver diseases. It is elucidated that Sipimikwanjung-tang has antioxidants on liver tissue of rat and the cytotoxic effects on human hepatoblastoma Hep G2 cells. Methods: Sipimikwanjung-tang extract in antioxidant effects of Hep G2 cells is evaluated by MTT assay, DAPI staining, DNA fragmentation assays and FACS can analysis. Results: Sipimikwanjung-tang induced apoptosis in Hep G2 cells, and induced G1 and G2M arrest of the cell cycle as well as a significant increase in PARP and caspase-3 activity. It induced an increase in $H_2O_2$ generation and the subsequent $NF-{\kappa}B$ activation and also induced cell apoptosis through the caspase-3-dependent pathways in the low concentration of Sipimikwanjung-tang extracts. However, the high dose of Sipimikwanjung-tang extract in Hep G2 cells inhibited $TGF-{\beta}l-induced$ apoptosis via increase in cellular $H_2O_2$, formation and $NF-{\kappa}B$ activation in human hepatoblastoma Hep G2 cells. Conclusion: From this study, the possibility that Sipimikwanjung-tang extracts apply to antioxidant and apoptotic treatment of disease is revealed.

The Effect of Livestock and Food Waste Compost on Rice Yield and Nutrient Utilization Efficiency according to Different Nitrogen Fertilizer Treatments Rates

Song Rae Cho,Ye-Jin Lee,Chan-Wook Lee,Byeong-Geun Hyun,Yo-Sung Song,Chul-Hyun Ryu,Yang Min Kim,Seul-Bi Lee 한국토양비료학회 2021 한국토양비료학회지 Vol.54 No.4

The amount of livestock manure generated was 1,532 hundred tons/day as of 2019, and it increased by about 11% over 10 years. It has been raised as a cause of soil and water pollution due to the continuous overspray of livestock compost on farmland. It is essential to calculate an appropriate amount of livestock compost that can replace chemical fertilizers in order to manage local environment-friendly nutrients without problems caused by the use of livestock compost. In addition, food waste is mostly agricultural products-derived materials, and it is necessary to establish a comprehensive management system for agricultural cycle use. In this study, to confirm the compost mixed with livestock and food waste as an alternative to chemical fertilizer, comparison of the basic replacement of chemical fertilizer and livestock, the replacement of the mixed compost including food waste, the replacement of the total nitrogen amount of livestock compost, and the replacement of the total nitrogen amount of the mixed compost. Experiments were conducted to confirm the effect of each compost on crop production. Commercially available livestock compost (_L) and mixed compost (food waste and live-stock)(_M) were used as the material for the experiment. Treatments were control, NPK, 5N_L, 5N_M, 7.5N_L, 7.5N_M, 9N_L, 9N_M. The comparison between livestock compost and mixed compost, mixed compost has 30% high crop productivity. The nitrogen use efficiency was shown as 7.5N_M > 5N_M > 5N_L > NPK > 7.5N_L > 9N_M > 9N_L. For high yield and nutrient uptake, fertilizer partial replacement (55.6% or 83.3% than total replacement) will be more efficient than 100% total nitrogen requirement replacement.

Unusual migration of ringed polytetrafluoroethylene middle hepatic vein graft into the stomach in a patient undergone living donor liver transplantation

Sung-Hwa Kang,Shin Hwang,Dong-Hwan Jung,Chul-Soo Ahn,Ki-Hun Kim,Deok-Bog Moon,Tae-Yong Ha,Gi-Won Song,Gil-Chun Park,Hyung-Woo Park,Yo-Han Park,Bo-Hyun Jung,Sung-Gyu Lee 한국간담췌외과학회 2013 한국간담췌외과학회 학술대회지 Vol.2013 No.4

Total extirpation of the extrahepatic bile duct for superficial bile duct malignancy

Yo-Han Park,Shin Hwang,Hyung-Woo Park,Sung-Hwa Kang,Bo-Hyun Jung,Gi-Won Song,Dong-Hwan Jung,Tae-Yong Ha,Chul-Soo Ahn,Ki-Hun Kim,Deok-Bog Moon,Gil-Chun Park,Sung-Gyu Lee 한국간담췌외과학회 2013 한국간담췌외과학회 학술대회지 Vol.2013 No.4

Is There Any Difference of Microorganism Between Past and Present in Acute Cholangitic Patients?

( Yo Han Jeong ),( Kyeong Ok Kim ),( Jay Song ),( Si Hyung Lee ),( Tae Nyeun Kim ),( Ho Chan Lee ),( Sung Bum Kim ),( Jae Hyun Park ),( Kook Hyun Kim ),( Byung Ik Jang ),( Se Hoon Sohn ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Recently, antibiotics resistant microorganism, particularly extended- spectrum beta-lactamase (ESBL)-producing organisms have been increased. Theaim of this study was to analyze the change of microorganism and origin of infection between past (2000-2006, group A) and present (2007-2013, group B) in patients with acute cholangitis. Methods: Of the acute cholangitic patients from Jan 2000 to Dec 2013, a systemic review of 896 patients having positive blood and/or bile culture (293 in group A vs. 603 in group B) was conducted retrospectively. They were further classifi ed into the two subgroups: community- and hospital-acquired infection. Bile culture was obtained via ENBD catheter. Results: In blood culture, the most common microorganism was E.coli [43.3% vs 41.8%] between group A and B. ESBL-producing E.coli was significantly higher in group B [13.6% vs 6.2%, p= 0.001]. The numbers of patients with bile culture-positive were 123 in group A and 196 in group B. In bile culture, the most common pathogen was E.coli [58.5% vs 45.4%] between group A and B. ESBL-producing E.coli was signifi cantly higher in group B [22.4% vs 4.8%, p=0.001]. The numbers of patients with community-acquired infection was higher than hospital-acquired infection (71.9% vs 28.9%, respectively). In community-acquired infection, ESBL-producing E.coli was signifi cantly higher in group B both in the blood culture [11.2% vs 5.6%, p=0.021] and in the bile culture [6.4% vs 2.3%, p=0.025]. In hospital-acquired infection, ESBL-producing E.coli was signifi cantly higher in group B both in the blood culture [19% vs 8.0%, p=0.028] and in the bile culture [9.2% vs 1.3%, p=0.023]. Conclusions: The numbers of ESBL-producing E.coli has been increased recently. The physicians must keep in mind that ESBL-producing E.coli is the causative pathogen in patients with acute cholangitis, irrespective of whether community or hospital-acquired infection.

Surgical results of only antegrade del Nido cardioplegia infusion in conventional coronary artery bypass grafting: a retrospective study

Sang-Uk Park,Yo Han Bae,김윤석,Kyungsub Song,장우성 영남대학교 의과대학 2023 Yeungnam University Journal of Medicine Vol.40 No.-

Background: Additional retrograde cardioplegia infusion in conventional coronary artery bypass grafting (CABG) was introduced to address the concern of inappropriate cardioplegia delivery through the stenotic coronary artery. However, this method is complex and requires repeated infusions. Therefore, we investigated the surgical outcomes of only antegrade cardioplegia infusion in conventional CABG. Methods: We included 224 patients who underwent isolated CABG between 2017 and 2019. The patients were divided into two groups according to the cardioplegia infusion method: antegrade cardioplegia infusion with del Nido solution (n=111, group I) and antegrade+retrograde cardioplegia infusion with blood cardioplegia solution (n=113, group II). Results: The sinus recovery time after release of the aorta cross-clamp was shorter in group I (3.8±7.1 minutes, n=98) than in group II (5.8±4.1 minutes, n=73) (p=0.033). The total cardioplegia infusion volume was lower in group I (1,998.6±668.6 mL) than in group II (7,321.0±2,865.3 mL) (p<0.001). Creatine kinase-MB levels were significantly lower in group I than in group II (p=0.039). Newly developed regional wall motion abnormalities on follow-up echocardiography were detected in two patients (1.8%) in group I and five patients (4.4%) in group II (p=0.233). There was no significant difference in ejection fraction improvement between the two groups (3.3%±9.3% in group I and 3.3%±8.7% in group II, p=0.990). Conclusion: The only antegrade cardioplegia infusion strategy in conventional CABG is safe and has no harmful effects.

Effects of Temperature and Time on the Melting and Crystallization of Polyesters(Ⅱ) : Poly(1,4-butanediol succinate) 폴리(1,4-부탄디올 숙시네이트)의 용융 및 결정화

Sung, Yoing Kiel,Song, Dae Kyung 東國大學校 1994 東國論叢 Vol.33 No.-

중합체의 유리전이온도, 용융온도 및 결정화 온도를 시차주사열량계를 이용하여 측정 분석하였다. 폴리(1,4-부탄디올 숙시네이트)의 유리전이온는 73℃에서 나타났으며, 다중용융현상을 나타내었다. 가열속도, 냉각속도, 재가열속도 및 분자량에 따른 중합체의 결정화 및 냉결정화에 대하여 조사하였다. 가열속도가 느릴수록 핵형성시간 및 중합체사슬의 재배열시간이 증가되므로 인하여 용융상태로부터 결정화 온도가 증가하였다. 등온과정에서의 결정화 속도에 대해 조사하고 핵형성 및 결정화에 정보를 주는 아브라미지수가 3이 됨을 확인하였다. 그리고 폴리(1,4-부탄디올 숙시네이트)로부터 형성된 구정은 말타십자형이었다. The glass transition temperature, melting, and crystallization temperatures of poly(1, 4-butanediol succinate) were measured by differential scanning calorimetry. The glass transition temperature of poly(1,4-butanediol succinate) was shown at 73℃ and melting endotherm revealed multiplet. The crystallization and cold crystallization of the polymers were investigated as a function of heating rate, cooling rate, reheating rate, and molecular weight. Slower scanning rates allowed more times for nucleation, reorganization, and packing of the polymer chain, so the onset temperature of crystallization from the melt was increased. The crystallization kinetics were also studied in isothermal condition. The Avrami exponent, n, which provides the information on the type of nucleation and crystallization occuring, was about 3. The spherulite formed in poly(1, 4-butanediol succinate) has a Maltese cross.

Poster Session:PS 1130;Gastroenterology (Liver):A Sarcomatoid Carcinoma Arising from Intrahepatic Cholangiocyte: An Unusual Primary Liver Cancer

( Yo Han Kim ),( Kyoung Hwang Shin ),( Sung Hwahn Hahn ),( Jae Hwan Kong ),( Joon Ho Choi ),( Seoung Min Jeon ),( Hyun Deok Shin ),( Suk Bae Kim ),( Jung Eun Shin ),( Hong Ja Kim ),( Il Han Song ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Sarcomatoid carcinoma arising from intrahepatic cholangiocyte, an extremely rare primary liver cancer, has highly invasive and metastatic potential. The pathogenesis of this tumor is unclear, although histogenetic mechanisms such as ‘transformation’, ‘combination’ and ‘collision’ might be suggested to explain the simultaneous co-existence of carcinoma and sarcoma components in the same tumor. Case: A 58 year-old male presented with a hepatic mass that was found during regular health examination. Liver computed tomography (CT) scan revealed 2. 0 cm-sized heterogeneous low density mass without enhancement located on the segment 6. Magnetic resonance imaging showed the tumor of segment 6 with a low-signal intensity in T1-weighted image and a high-signal intensity in T2-weighted image. Positron emission tomography-CT showed a focal hypermetabolic lesion in the same area, with no evidence of distant metastasis. This mass was suspected to be intrahepatic cholangiocarcinoma by ultrasonography-guided needle biopsy, so right posterior sectionectomy was performed. The resected liver showed a well-defi ned whitish or yellowish gray solid mass with a central hemorrhage and necrosis at a subcapsular area of segment 6. Microscopically, the tumor tissue consists of multiple polygonal and pleomorphic cells. The tumor cells were immunohistochemically positive for cytokeratin 19 and vimentin, but negative for Hep-par 1. The patient was confi rmed a defi nitive diagnosis of intrahepatic sarcomatoid carcinoma. After 2 months, the patient developed extensive abdominal metastases and received systemic chemoradiotherapy, resulting in no response visible radiologically. Conclusions: We report an unusual case of sarcomatoid carcinoma of the liver arising from intrahepatic cholangiocyte, confi rmed by immunohistochemical analysis following surgical resection.

The Effect of cilostazolon Glucose Tolerance and Insulin Resistance in a Rat Model of Non - insulin Dependent Diabetes Mellitus

(Sang Ah Chang),(Bong Yun Cha),(Soon Jib Yoo),(Yo Bae Ahn),(Ki Ho Song),(Je Ho Han),(Jong Min Lee),(Hyun Sik Son),(Kun Ho Yoon),(Moo Il Kang),(Kwang Woo Lee),(Ho Young Son),(Sung Ku Kang) 대한내과학회 2001 The Korean Journal of Internal Medicine Vol.16 No.2

N/A Background : It has been reported that many peripheral vasodilating drugs might improve insulin resistance. Cilostazol, a antithrombotic agent, increases peripheral blood flow in non-insulin dependent diabetic patients. The effect of cilostazol treatment on insulin resistance in streptozotocin (STZ)-induced non-insulin dependent diabetic Wistar rats was examined. Methods : About a half of two-day old neonate siblings were injected intraperitoneally with STZ and maintained for six months, at which time they were compared with age-matched control rats for intraperitoneal glucose tolerance test (IPGTT) and for glucose infusion rate (GINF) in a euglycemic hyperinsulinemic glucose-clamp study. After that, these studies were also performed after feeding rat chow containing cilostazol (100 mg/kg/day) to rats with STZ-induced non-insulin dependent diabetes mellitus for four-weeks and compared with those of age-matched control rats. Results : In the intraperitoneal glucose tolerance test studies, plasma glucose levels of STZ-induced non-insulin dependent diabetic rats were significantly higher and plasma insulin levels significantly lower than those of age-matched control rats in the age of six months. Glucose infusion rate was lower in STZ-induced non-insulin dependent diabetic rats than those of age-matched control rats. However, after a four-week cilostazol treatment, glucose infusion rate of STZ-induced non-insulin dependent diabetic rats was not significantly different from that of control rats. Conclusion : These findings suggested that cilostazol may improve insulin resistance in STZ-induced non-insulin dependent diabetic rats.

Characterization of Cell Growth and Camptothecin Production in Cell Cultures of Camptotheca acuminata

Song, Seung-Hoon,Byun, Sang-Yo The Korean Society for Microbiology and Biotechnol 1998 Journal of microbiology and biotechnology Vol.8 No.6

Studies were made to elucidate the cell growth and the production of camptothecin and its derivatives in cell cultures of Camptotheca acuminata. High resolution HPLC chromatograms to analyze camptothecin and 10-hydroxycamptothecin in lactone and carboxylate forms were obtained with a fluorescence detector. Calli inductions were optimized with the young stem of explant on Schenk and Hildebrandt (SH) medium supplemented with 5 mg/l $\alpha$-naphthaleneacetic acid (NAA), 0.2 mg/l 6-benzylamino purine (BAP), 2.0% sucrose, and 0.5% agar. The hybrid medium, a mixture of SH and Murashige and Skoog (MS) salts, was developed for homogeneous suspension cultures without large cell aggregates. The optimum phytohormone concentrations for successful suspension cultures were 1.0mg/l of 2,4-D and 0.5 mg/l of kinetin. The highest growth in suspension cultures was observed when 49.7% (w/w) of the cells was composed of small aggregates which were below 0.1 mm in diameter. Time course changes of cell growth and camptothecin production showed that camptothecin accumulation was started at the end of the growth phase and the maximum content was obtained 10 days after inoculation. Yeast extract elicitor increased camptothecin accumulation 4 times. Methyl jasmonate and jasmonic acid also increased camptothecin production 6 and 11 times, respectively.