Identification of a novel circularized transcript of the AML1 gene

Ai Ning Xu,Xiu Hua Chen,Yan Hong Tan,Xi Ling Qi,Zhi Fang Xu,Lin Lin Zhang,Fang Gang Ren,Si Cheng Bian,Yi Chen,Hong Wei Wang 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.3

Prognostic Significance of Desmoglein 2 and Desmoglein 3 in Esophageal Squamous Cell Carcinoma

Fang, Wang-Kai,Gu, Wei,Liao, Lian-Di,Chen, Bo,Wu, Zhi-Yong,Wu, Jian-Yi,Shen, Jian,Xu, Li-Yan,Li, En-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objective: Desmogleins (DSGs) are major members among the desmosomal cadherins critically involved in cell-cell adhesion and the maintenance of normal tissue architecture in epithelia. Reports exploring links of DSG family member expression with cancers are few and vary. The aim of this study was to investigate the ratio of DSG2 and DSG3 mRNA expression in esophageal squamous cell carcinoma (ESCC) tissue to normal tissue (T/N ratio) and evaluate correlations with clinical parameters. Methods: The mRNA expression of DSGs, as well as ${\gamma}$-catenin and desmoplakin, was detected by real-time quantitative RT-PCR in 85 cases of ESCC tissue specimens. Results: The expression level of DSG3 mRNA was significantly higher than that of DSG2 in ESCC specimens (p=0.000). DSG3 mRNA expression highly correlated with histological grade (p=0.009), whereas that of DSG2 did not significantly relate to any clinicopathologic parameter. Kaplan-Meier survival analysis showed that only DSG3 expression had an impact on the survival curve, with negative DSG3 expression indicating worse survival (p=0.038). Multivariate Cox regression analysis demonstrated DSG3 to be an independent prognostic factor for survival. Furthermore, correlation analysis demonstrated the mRNA level of DSG3 to highly correlate with those of ${\gamma}$-catenin and desmoplakin in ESCC samples (p=0.000), implying that the expression of desmosomal components might be regulated by the same upstream regulatory molecules. Conclusions: Our findings suggest that DSG3 may be involved in the progression of ESCC and serve as a prognostic marker, while expression of DSG2 cannot be used as a predictor of ESCC patient outcome.

Selective extraction of thorium to directly form self-assembly solid from HNO3 solution

Fang Zhang,Qiang Wu,Lei-Tao Sha,Yang Li,Xu-Xin Li,Ze-Yang Wang,Xuan Fu,Qing-Gang Huang,Bin Liu,Ze-Yi Yan 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

Based on ions exchange between [DMDSA]+[Cl]- (Dimethyl distearyl ammonium chloride) and N,Ndialkyl-succinamide acid (SCA), three novel bifunctional [DMDSA]+[SCA]- ionic liquids (ILs) were firstlysynthesized for extraction of thorium (IV) by self-assembly strategy. The simultaneous extraction andsolidification of Th(IV) were unexpectedly realized in one-step operation using the present ILs in HNO3solution, and more than 99% thorium (IV) was enriched and immediately aggregated into selfassemblysolid at the biphasic interface. The self-assembly solid was further identified by FT-IR, SEM withelement mapping EDS and XPS analysis, and revealing that the self-assembly extraction (SAE) was triggeredby the amphiphilic [DMDSA]+ cations. A three-step extraction mechanism dominated by [SCATh(NO3)4]- was proposed based on the slope analysis method and HRMS analysis. The self-assembly extractionof Th(IV) exhibited the extremely excellent selectivity in the presence of U(VI) and typical lanthanideelements including La(III), Eu(III) and Lu(III), and the separation factors reached 2516 for Th/U, 1885 forTh/La, 1512 for Th/Eu and 558 for Th/Lu, respectively. The proposed SAE strategy was proved to be anefficient method for one-step separation and solidification of thorium ions from U(VI) and/or lanthanides.

RhGLP-1 (7-36) protects diabetic rats against cerebral ischemia-reperfusion injury via up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD

Fang, Yi,Liu, Xiaofang,Zhao, Libo,Wei, Zhongna,Jiang, Daoli,Shao, Hua,Zang, Yannan,Xu, Jia,Wang, Qian,Liu, Yang,Peng, Ye,Yin, Xiaoxing The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5

The present study aimed to explore the neuroprotective effect and possible mechanisms of rhGLP-1 (7-36) against transient ischemia/reperfusion injuries induced by middle cerebral artery occlusion (MCAO) in type 2 diabetic rats. First, diabetic rats were established by a combination of a high-fat diet and low-dose streptozotocin (STZ) (30 mg/kg, intraperitoneally). Second, they were subjected to MCAO for 2 h, then treated with rhGLP-1 (7-36) (10, 20, $40{\mu}g/kg$ i.p.) at the same time of reperfusion. In the following 3 days, they were injected with rhGLP-1 (7-36) at the same dose and route for three times each day. After 72 h, hypoglycemic effects were assessed by blood glucose changes, and neuroprotective effects were evaluated by neurological deficits, infarct volume and histomorphology. Mechanisms were investigated by detecting the distribution and expression of the nuclear factor erythroid-derived factor 2 related factor 2 (Nrf2) in ischemic brain tissue, the levels of phospho-PI3 kinase (PI3K)/PI3K ratio and heme-oxygenase-1 (HO-l), as well as the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA). Our results showed that rhGLP-1 (7-36) significantly reduced blood glucose and infarction volume, alleviated neurological deficits, enhanced the density of surviving neurons and vascular proliferation. The nuclear positive cells ratio and expression of Nrf2, the levels of P-PI3K/PI3K ratio and HO-l increased, the activities of SOD increased and the contents of MDA decreased. The current results indicated the protective effect of rhGLP-1 (7-36) in diabetic rats following MCAO/R that may be concerned with reducing blood glucose, up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD.

RhGLP-1 (7-36) protects diabetic rats against cerebral ischemia-reperfusion injury via up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD

Yi Fang,Xiaofang Liu,Libo Zhao,Zhongna Wei,Daoli Jiang,Hua Shao,Yannan Zang,Jia Xu,Qian Wang,Yang Liu,Ye Peng,Xiaoxing Yin 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5

The present study aimed to explore the neuroprotective effect and possible mechanisms of rhGLP-1 (7-36) against transient ischemia/reperfusion injuries induced by middle cerebral artery occlusion (MCAO) in type 2 diabetic rats. First, diabetic rats were established by a combination of a high-fat diet and low-dose streptozotocin (STZ) (30 mg/kg, intraperitoneally). Second, they were subjected to MCAO for 2 h, then treated with rhGLP-1 (7-36) (10, 20, 40 μg/kg i.p.) at the same time of reperfusion. In the following 3 days, they were injected with rhGLP-1 (7- 36) at the same dose and route for three times each day. After 72 h, hypoglycemic effects were assessed by blood glucose changes, and neuroprotective effects were evaluated by neurological deficits, infarct volume and histomorphology. Mechanisms were investigated by detecting the distribution and expression of the nuclear factor erythroid-derived factor 2 related factor 2 (Nrf2) in ischemic brain tissue, the levels of phospho-PI3 kinase (PI3K)/PI3K ratio and heme-oxygenase-1 (HO-l), as well as the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA). Our results showed that rhGLP-1 (7-36) significantly reduced blood glucose and infarction volume, alleviated neurological deficits, enhanced the density of surviving neurons and vascular proliferation. The nuclear positive cells ratio and expression of Nrf2, the levels of P-PI3K/PI3K ratio and HO-l increased, the activities of SOD increased and the contents of MDA decreased. The current results indicated the protective effect of rhGLP-1 (7-36) in diabetic rats following MCAO/R that may be concerned with reducing blood glucose, up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD.

Liver Cancer Mortality Characteristics and Trends in China from 1991 to 2012

Fang, Jia-Ying,Wu, Ku-Sheng,Zeng, Yang,Tang, Wen-Rui,Du, Pei-Ling,Xu, Zhen-Xi,Xu, Xiao-Ling,Luo, Jia-Yi,Lin, Kun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Purpose: To investigate the distribution of liver cancer mortality as well as its developing trend from 1991 to 2012, forecast the future five-year trend, and provide a basis for the comprehensive prevention and management. Materials and Methods: Mortality data for liver cancer in China from 1991 to 2012 were used to describe characteristics and distribution of liver cancer mortality. Trend surface analysis was used to study the geographical distribution of liver cancer mortality. Curve estimation, time series modeling, gray modeling (GM) and joinpoint regression were used to predict and forecast future trends. Results: The mortality rate of liver cancer has constantly increased in China since 1991. Rates in rural areas are higher than in urban areas, and in males are higher than in females. In addition, our data predicted that the trend will continue to increase in the next 5 years. The age-specific mortality of liver cancer increases with age and peaks in the group of 80-84 years old. Geographical analysis showed the liver mortality rate was higher in the southeast provinces, such as Jiangsu, Zhejiang and Guangdong, and southwest regions like Guangxi Province. Conclusions: The standardized mortality rate of liver cancer in China has consistently increased from 1991 to 2012, and the upward trend is predicted to continue in the future. Much better prevention and management of liver cancer is needed in high mortality areas (the southwestern and southeastern parts of China) and high mortality age groups (80- to 84-year-olds), especially in rural areas.

Enhanced Reactive Dye Inkjet Printing Performance of Antimicrobial Silk Fabrics Surface Modified with Plasma and Chitosan

Yi Xu,Kuanjun Fang,Weichao Chen,Xiangwu Zhang,Chunming Zhang 한국섬유공학회 2022 Fibers and polymers Vol.23 No.9

This paper develops a new clean production technology combining atmospheric pressure plasma jets and nanochitosanto prepare strong and durable bio-based coatings for improving the inkjet printing performance of antimicrobial silkfabrics. The key contribution of this study is to introduce green and environmental friendly production methods, avoiding theuse of chemicals and pollution caused by dye hydrolysis in traditional ways. By contradistinctive experiments, we presentdirect empirical evidence that the plasma-chitosan treatment can significantly improve the hydrophilicity of the silk surfaces. Wettability test indicates that the infiltration time of droplet on the surface of modified silk fabric was only 1/12 of the timespent on the untreated sample. The study of reactive dye inkjet printing illustrates that the antibleeding property on modifiedsamples was evidently enhanced. Meanwhile, in respect of color fixation of reactive dyes, there was also an increase in K/Svalue on modified samples, ranging from 76 % to 214 % in color depth of the four-color print blocks, even after gas steamingand washing. Scanning electron microscope (SEM) observation reveals that the surface of silk fibers modified with plasma iscovered with firmer and more uniform chitosan coatings in terms of morphology change. X-ray photoelectron spectroscopy(XPS) analysis verifies that the content of oxygenous groups on the silk fabric processed by plasma-chitosan increased bymore than 42 %. As to the antibacterial effect of nano-chitosan, the photographs of inhibition zone shows that the plasmaprocessing effectively enhanced the durability of chitosan against B. subtilis, and thus prepared long-lasting antibacterial silkfabric. Furthermore, measurements of washing and rubbing color fastness prove that the plasma-chitosan treated fabrics stillhad excellent inkjet printing effect even after 45 cycles of soaping. Taken together, it turns out that such nontoxic,nonpolluting, low energy-consumed chitosan coating combining with plasma can explicitly provide an innovative option forthe production of inkjet printing on natural fabrics.

The Effects of Allitridin on the Expression of Transcription Factors T-bet and GATA-3 in Mice Infected by Murine Cytomegalovirus

Xu Yi,Fang Feng,Zhidan Xiang,Li Ge 한국식품영양과학회 2005 Journal of medicinal food Vol.8 No.3

This study was designed to investigate the effects of allitridin on the expression of transcription factors T-betand GATA-3 in mice infected by murine cytomegalovirus (MCMV). A BALB/c mouse model system of MCMV infectionwas established. Twenty mice were allocated randomly into an allitridin-treated group (n . 10) and a placebo control group(n . 10). The same dose (25 mg/kg/day) and regimen of allitridin were used in the treated group in the 24 hours after virusinfection; the same volume of saline solution was injected in placebo control mice. In an additional blank control group (n .10), the same volume of saline solution was injected. The expression levels of the transcription factors T-bet and GATA-3were measured by reverse transcriptionpolymerase chain reaction. The expression levels of the T helper (Th) 1 cytokine in-terferon-. (IFN-.) and the Th2 cytokine interleukin (IL)-10 in supernatant of spleen cell culture were measured by enzyme-linked immunosorbent assay. MCMV infection markedly down-modulated the expression of IFN-. and T-bet and significantlyup-modulated the expression of IL-10 and GATA-3. Allitridin induced significantly (P. .01) increased expression of thetranscription factor T-bet and the Th1 cytokine IFN-. and markedly (P. .01) decreased expression of the transcription fac-tor GATA-3 and the Th2 cytokine IL-10. Thus MCMV infection could lead to disequilibrium of Th1/Th2 cytokine expres-sion: The level of the Th1 cytokine IFN- was decreased significantly, and Th2 cytokine IL-10 was overexpressed markedly.Allitridin could up-regulate the expression of T-bet and IFN-. and inhibit the expression of GATA-3 and IL-10 in MCMV-infected mice, indicating a Th1 dominant state, which should enhance the specific cellular immune reactions against cy-tomegalovirus (CMV) and be helpful for clearance of CMV from the host.

Activation of JNKs is essential for BMP9-induced osteogenic differentiation of mesenchymal stem cells

Yan Fang Zhao,Jing Xu,Wen Juan Wang,Jin Wang,Juan Wen He,Li Li,Qian Dong,Yan Xiao,Xing Lian Duan,Xue Yang,Yi Wen Liang,Tao Song,Min Tang,Dan Zhao,Jin Yong Luo 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.8

The Effect of Alkali Treatment on the Crystallinity, Thermal Stability, and Surface Roughness of Bamboo Fibers

Xiaoyang Fang,Jianuo Xu,Hongwu Guo,Yi Liu 한국섬유공학회 2023 Fibers and polymers Vol.24 No.2

The bamboo fibers were treated with sodium hydroxide (NaOH) to investigate the impacts of the NaOH concentration, reactiontemperature, and soaking time on the crystallinity, thermal stability, and surface roughness of bamboo fibers. The resultsrevealed that the NaOH concentration had a significant impact (0.01 < P < 0.05) on the crystallinity and thermal stability ofbamboo fibers, as well as an extremely significant impact (P < 0.01) on their surface roughness. It was found an extremelysignificant (P < 0.01) effect of the reaction temperature in the crystallinity and surface roughness of bamboo fibers but notin thermal stability. The soaking time significantly (P < 0.01) affected the surface roughness of bamboo fibers, whereas nosignificant effects were observed for the crystallinity and thermal stability. Compared with untreated bamboo fibers, thecrystallinity, thermal stability, and surface roughness of alkali-treated bamboo fibers generally increased.