Promoter Analysis of Bombyx mori Nucleopolyhedrovirus Ubiquitin Gene

Xu’ai Lin,Yin Chen,Yongzhu Yi,Jie Yan,Zhifang Zhang 한국미생물학회 2008 The journal of microbiology Vol.46 No.4

The aim of this study was to analyze the characteristics of Bombyx mori nucleopolyhedrovirus (BmNPV) ubiquitin gene promoter and the effects of conserved motifs, such as TAAG, TATA, and CAAT, along with baculovirus enhancer homologous region 3 (hr3), on promoter activity. Ubiquitin gene of BmNPV was expressed during the late phase of virus infection. In the presence of viral factors, significant reduction of promoter activity was observed by deletion of -382 to -124 bp upstream of ATG. The fragment between -187 and -383 bp upstream of ATG, including distal TAAG, CAAT motif, and TATA box, could also drive expression of the reporter gene. The mutation of cis-elements TATA boxes and TAAG motifs significantly decreased the promoter’s activity, while CAAT mutations enhanced promoter activity by 2- or 3-fold, as compared with the native promoter. In the presence of BmNPV, hr3, both located downstream of the reporter gene of the same vector, and separate vector, could significantly enhance transcription activity of ubiquitin promoter as compared to the control. We concluded that BmNPV ubiquitin gene might be regulated by dual sets of promoter elements, where TAAG and TATA box may positively regulate the expression of ubiquitin, while CAAT motif functions as a negative regulator. Viral factor(s) play an important role in the co-activation of hr3 and promoter.

Cloning, Expression, and Functional Characterization of the Dunaliella salina 5-enolpyruvylshikimate-3-phosphate Synthase Gene in Escherichia coli

Yi, Yi,Qiao, Dairong,Bai, Linhan,Xu, Hui,Li, Ya,Wang, Xiaolin,Cao, Yi The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.2

5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19) is the sixth enzyme in the shikimate pathway which is essential for the synthesis of aromatic amino acids and many secondary metabolites. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide glyphosate. In this study, the Dunaliella salina EPSP synthase gene was cloned by RT-PCR approach. It contains an open reading frame encoding a protein of 514 amino acids with a calculated molecular weight of 54.6 KDa. The derived amino acid sequence showed high homology with other EPSP synthases. The Dunaliella salina EPSP synthase gene was expressed in Escherichia coli and the recombinant EPSP synthase were identified by functional complementation assay.

The <i>Arabidopsis</i> NAC Transcription Factor ANAC096 Cooperates with bZIP-Type Transcription Factors in Dehydration and Osmotic Stress Responses

Xu, Zheng-Yi,Kim, Soo Youn,Hyeon, Do Young,Kim, Dae Heon,Dong, Ting,Park, Youngmin,Jin, Jing Bo,Joo, Se-Hwan,Kim, Seong-Ki,Hong, Jong Chan,Hwang, Daehee,Hwang, Inhwan American Society of Plant Biologists 2013 The Plant cell Vol.25 No.11

<P>This work examines the role of the NAC transcription factor ANAC096, finding that ANAC096 interacts with specific bZIP transcription factors to globally affect abscisic acid–responsive transcription during osmotic and drought stresses.</P>

Enhanced Reactive Dye Inkjet Printing Performance of Antimicrobial Silk Fabrics Surface Modified with Plasma and Chitosan

Yi Xu,Kuanjun Fang,Weichao Chen,Xiangwu Zhang,Chunming Zhang 한국섬유공학회 2022 Fibers and polymers Vol.23 No.9

This paper develops a new clean production technology combining atmospheric pressure plasma jets and nanochitosanto prepare strong and durable bio-based coatings for improving the inkjet printing performance of antimicrobial silkfabrics. The key contribution of this study is to introduce green and environmental friendly production methods, avoiding theuse of chemicals and pollution caused by dye hydrolysis in traditional ways. By contradistinctive experiments, we presentdirect empirical evidence that the plasma-chitosan treatment can significantly improve the hydrophilicity of the silk surfaces. Wettability test indicates that the infiltration time of droplet on the surface of modified silk fabric was only 1/12 of the timespent on the untreated sample. The study of reactive dye inkjet printing illustrates that the antibleeding property on modifiedsamples was evidently enhanced. Meanwhile, in respect of color fixation of reactive dyes, there was also an increase in K/Svalue on modified samples, ranging from 76 % to 214 % in color depth of the four-color print blocks, even after gas steamingand washing. Scanning electron microscope (SEM) observation reveals that the surface of silk fibers modified with plasma iscovered with firmer and more uniform chitosan coatings in terms of morphology change. X-ray photoelectron spectroscopy(XPS) analysis verifies that the content of oxygenous groups on the silk fabric processed by plasma-chitosan increased bymore than 42 %. As to the antibacterial effect of nano-chitosan, the photographs of inhibition zone shows that the plasmaprocessing effectively enhanced the durability of chitosan against B. subtilis, and thus prepared long-lasting antibacterial silkfabric. Furthermore, measurements of washing and rubbing color fastness prove that the plasma-chitosan treated fabrics stillhad excellent inkjet printing effect even after 45 cycles of soaping. Taken together, it turns out that such nontoxic,nonpolluting, low energy-consumed chitosan coating combining with plasma can explicitly provide an innovative option forthe production of inkjet printing on natural fabrics.

A Comparison of Transradial and Transfemoral Percutaneous Coronary Intervention in Chinese Women Based on a Propensity Score Analysis

Yi Xu,Chen Jin,Shubin Qiao,Yongjian Wu,Hongbing Yan,Kefei Dou,Bo Xu,Jingang Yang,Yuejin Yang 대한심장학회 2018 Korean Circulation Journal Vol.48 No.8

Background and Objectives: Over the past decades, transradial approach for percutaneous coronary intervention (PCI) has been increasingly adopted in clinical practice. Women represent a large population who will possibly benefit from PCI, but they are often under-represented in clinical studies. Therefore, the role of TRI in women remains to be further defined. This study sought to compare safety and efficacy for transradial intervention (TRI) and transfemoral intervention (TFI) in women undergoing PCI in China. Methods: The study population consisted of 5,067 women undergoing PCI at Fuwai Hospital, Beijing, China between 2006 and 2011 (TRI: n=4,105, TFI: n=962). Incidence rates of clinical outcomes during hospitalization and at 1-year follow-up were compared between TRI and TFI. In order to minimize potential biases, a 1:1 propensity score matching (PSM) was performed. A total of 899 pairs were matched. Results: Baseline and procedural characteristics were well-balanced between TRI and TFI groups after controlling for confounders using PSM. TRI was associated with reduced major post-PCI bleeding (odds ratio [OR], 0.64; 95% confidence interval [CI], 0.54–0.76; p<0.001) and access site complications (OR, 0.67; 95% CI, 0.61–0.74; p<0.001) after PSM. There was no statistical differences in the incidence rates of major adverse cardiac events (a composite of cardiac death, myocardial infarction, and target vessel revascularization) both during hospitalization and at 1-year follow-up (p>0.05). Conclusions: In this propensity score-based analysis of TRI versus TFI in Chinese women, TRI showed advantages of safety and feasibility over TFI. A wider adoption of TRI in women has the potential to improve outcomes in treatment of coronary artery diseases.

The Effects of Allitridin on the Expression of Transcription Factors T-bet and GATA-3 in Mice Infected by Murine Cytomegalovirus

Xu Yi,Fang Feng,Zhidan Xiang,Li Ge 한국식품영양과학회 2005 Journal of medicinal food Vol.8 No.3

This study was designed to investigate the effects of allitridin on the expression of transcription factors T-betand GATA-3 in mice infected by murine cytomegalovirus (MCMV). A BALB/c mouse model system of MCMV infectionwas established. Twenty mice were allocated randomly into an allitridin-treated group (n . 10) and a placebo control group(n . 10). The same dose (25 mg/kg/day) and regimen of allitridin were used in the treated group in the 24 hours after virusinfection; the same volume of saline solution was injected in placebo control mice. In an additional blank control group (n .10), the same volume of saline solution was injected. The expression levels of the transcription factors T-bet and GATA-3were measured by reverse transcriptionpolymerase chain reaction. The expression levels of the T helper (Th) 1 cytokine in-terferon-. (IFN-.) and the Th2 cytokine interleukin (IL)-10 in supernatant of spleen cell culture were measured by enzyme-linked immunosorbent assay. MCMV infection markedly down-modulated the expression of IFN-. and T-bet and significantlyup-modulated the expression of IL-10 and GATA-3. Allitridin induced significantly (P. .01) increased expression of thetranscription factor T-bet and the Th1 cytokine IFN-. and markedly (P. .01) decreased expression of the transcription fac-tor GATA-3 and the Th2 cytokine IL-10. Thus MCMV infection could lead to disequilibrium of Th1/Th2 cytokine expres-sion: The level of the Th1 cytokine IFN- was decreased significantly, and Th2 cytokine IL-10 was overexpressed markedly.Allitridin could up-regulate the expression of T-bet and IFN-. and inhibit the expression of GATA-3 and IL-10 in MCMV-infected mice, indicating a Th1 dominant state, which should enhance the specific cellular immune reactions against cy-tomegalovirus (CMV) and be helpful for clearance of CMV from the host.

Five new anthraquinones from the seed of Cassia obtusifolia

Yi-Long Xu,Li-Ying Tang,Xi-Dan Zhou,Guo-Hong Zhou,Zhu-Ju Wang 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

A phytochemical investigation on the seeds ofCassia obtusifolia led to the isolation of five new anthraquinones,including one aglycon and four glycosides. Thestructures were elucidated by analysis of extensive spectroscopicdata and the results of acid hydrolysis. All theseisolates were evaluated for their inhibitory effects againsta-glucosidase, and 1 showed potent activity with IC50value of 185 ± 15 lM.

朝鮮使者眼中的淸代戱曲

( Yi Xu ) 연민학회 2010 연민학지 Vol.14 No.-

본고는 조선시기 『燕行錄』중의 희곡 관련 자료를 연구대상으로 하여, 이국자의 시각으로 본 청대 희곡의 연극 활동 상황을 고찰하여 본다. 본고는 우선 『燕行錄』중에 청대 희곡의 연출 환경에 대한 묘사를 검토 분석하고, 그 다음 희곡 연출 복식에 대한 조선 사신들의 관심에 대해 논술하고, 마지막으로 『燕行錄』에 기록된 희곡 극목에 근거하여 청대 민간 희곡과 궁정 희곡의 분야를 서술해보려고 한다. 이런 보기드문 희곡 자료에 대한 정리를 통해 청대 희곡 연구의 시야를 넓혀보려고 한다. The research object of this paper is the Chinese Opera materials that are contained in YanXingLu (Travelogues of Korean Envoys to China). The activities of the Chinese opera are depicted through the perspective of Li Dynasty`s scholars. Firstly, I will analyze the environment of the performance recorded by the Korean envoys. Secondly, I will explain the reason that Korean scholars pay attention to the costume of the Chinese Opera. Finally, I will illustrate the differences between the folk drama and the palace opera. We could expand our research sight on the Qing Dynasty Opera by analyzing these ancient art works.

Enhanced Photocatalytic Performance of Heterojunction BiOI/BiOIO3 Nanocomposites Under Simulated Solar Light

Yi Ling Qi,Xu Chun Song,Yi Fan Zheng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.3

Novel heterostructure BiOI/BiOIO3 nanocomposites were successfully prepared through a facile deposition method at room temperature. BiOIO3 is a noncentrosymmetric compound that has an internal self-built electric field. BiOI was applied as a visible light absorber to sensitize semiconductors owing to its smallest bandgap. The coupling between BiOIO3 and BiOI can combine their advantages and improve photocatalytic properties. Compared with the single BiOI and BiOIO3, the heterostructure BiOI/BiOIO3 nanocomposites displayed a significantly enhanced photocatalytic activity for the Rhodamine B (RhB) degradation. The enhanced photocatalytic performance is deduced closely related to the formation of BiOI/BiOIO3 heterojunction interface whose presence is regarded to be a favorable factor for the transfer and separation of the photogenerated electrons and holes.

十八世紀朝淸文士筆談內容考述

( Yi Xu ),( Li Chen ) 한국외국어대학교 중국연구소 2014 中國硏究 Vol.60 No.-

The 18th century is a period when Qing and Choson literati communicate frequently with a variety of communicating forms such as conversations by writing, the exchange of poetry, reciprocating letters, asking for providing preface and postscript, literary criticism as well as giving books as presents, among which the conversations by writing is the most popular way of interaction. According to statistical study by the author, at least 193 detailed conversations by writing happened in the 18th century were recorded in the extant Yeonhaeng Rok, one of Chinese and Korean ancient scriptures. Its contents mainly covered ten aspects, in which some in-depth exchange was recorded. Therefore, these materials are of great literature value. Regarding to the communication of etiquette system and custom, Qing people`s comments on Choson ambassadors` clothing and inturn Choson views of binding feet of Qing women are always the key points. Besides, it also includes the communication of imperial examination system in Qing Dynasty and Ancient Korea;the discussion about academy and literary between Qing and Choson scholars; Choson literati` concerns with religion; Choson literati` focus on the Ming historical events, Qing political and agricultural situation as well associal development; Choson literati` acquaintance with compilation of books in Qing Dynasty, for example "The Imperial Collection of Four Treasures";the exchange of banned books in Qing Dynasty between Qing and Choson literati; Choson scholars` care for the landscape, geography of China and places of interest in China. Qing people also pay attention to Choson in many regards, for instance, landscape and geographical situation, scene style and feature views, ancient authors and their works, history, etiquette, custom, education, languageetc. It is obvious to find the diversity of their conversation topics. And these topics are closely associated with the culture, custom, history and the focus on Confucianism of Qing Dynasty and Choson. It should also be emphasized that no matter how abundant the conversations are, the key point is always related to concerns of Choson literati when visiting Qing Dynasty and their own scholar traits.