발아 및 발아온도가 커피의 산화방지 활성에 미치는 영향

임예서(Yeseo Lim),신용국(Yong Kook Shin),김도완(Do Wan Kim) 한국식품과학회 2018 한국식품과학회지 Vol.50 No.2

커피 생두의 발아 유무와 발아온도에 따른 커피 내 산화방지물질과 산화방지 활성 변화에 대해 살펴보았다. 20℃ 발아 커피는 미발아 커피에 비해 총 폴리페놀과 플라보노이드 함량이 높았고, DPPH와 ABTS 제거능 또한 높게 나타났다. 40℃ 발아 커피는 20℃ 발아커피에 비해 총 폴리페놀과 플라보노이드 함량이 유의적으로 낮았다. 특히, 40℃ 발아 커피는 미발아 커피와 비교했을 때에도 총 폴리페놀 함량이 낮은 것을 확인할 수 있었다. 커피 내 플라보노이드 함량과 산화방지 활성은 강한 양의 상관관계를 보였으나, 총 폴리페놀 함량은 산화방지 활성과 낮은 상관관계를 보였다. 이는 미발아 커피에 비해 40℃ 발아 커피에서 총 폴리페놀 함량이 유의적으로 감소했음에도 불구하고, DPPH제거능은 유의적으로 증가하였고, ABTS 제거능은 유의적 차이를 보이지 않은 것과 연관된다. 이상의 결과를 종합해보면, 커피생두의 발아는 커피 내 산화방지 물질과 산화방지 활성을 높이며, 적절한 발아온도의 설정은 이러한 커피의 생리활성을 극대화할 수 있는 중요한 요소라 할 수 있다. The present study aimed to investigate the effect of germination and temperature on the antioxidant activity of coffee. Green beans were germinated at 20 and 40℃. Germinated green beans were dried and roasted. Ground coffee was brewed at 90℃ for 5 min. Coffee samples were analyzed for antioxidant compounds and for its antioxidant activity. The total polyphenol content (TPC) and total flavonoid content (TFC) in coffee brewed with coffee beans germinated at 20℃ (CG20) were significantly higher than those in coffee brewed with non-germinated coffee beans (CNG). The same tendency was observed on 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging assays. TPC and TFC of coffee brewed with germinated coffee beans decreased with an increase in germination temperature from 20 to 40℃. In conclusion, germination of coffee beans contributes to an increase in its antioxidant activity. However, setting the appropriate temperature for germination is an important factor in determining the antioxidant activity of coffee.

Impact of Korean pine nut oil on weight gain and immune responses in high-fat diet-induced obese mice

Soyoung Park,Yeseo Lim,Sunhye Shin,Sung Nim Han 한국영양학회 2013 Nutrition Research and Practice Vol.7 No.5

Korean pine nut oil (PNO) has been reported to have favorable effects on lipid metabolism and appetite control. We investigated whether PNO consumption could influence weight gain, and whether the PNO-induced effect would result in an improvement of immune function in high-fat diet (HFD)-induced obese mice. C57BL/6 mice were fed control diets with 10% energy fat from either PNO or soybean oil (SBO), or HFDs with 45% energy fat from 10% PNO or SBO and 35% lard, 20% PNO or SBO and 25% lard, or 30% PNO or SBO and 15% lard for 12 weeks. The proliferative responses of splenocytes upon stimulation with concanavalin A (Con A) or lipopolysaccharide (LPS), Con A-stimulated production of interleukin (IL)-2 and interferon (IFN)-γ, and LPS-stimulated production of IL-6, IL-1β, and prostaglandin E2 (PGE2) by splenocytes were determined. Consumption of HFDs containing PNO resulted in significantly less weight gain (17% less, P < 0.001), and lower weight gain was mainly due to less white adipose tissue (18% less, P = 0.001). The reduction in weight gain did not result in the overall enhancement in splenocyte proliferation. Overall, PNO consumption resulted in a higher production of IL-1β (P = 0.04). Replacement of SBO with PNO had no effect on the production of IL-2, IFN-γ, IL-6, or PGE2 in mice fed with either the control diets or HFDs. In conclusion, consumption of PNO reduced weight gain in mice fed with HFD, but this effect did not result in the overall improvement in immune responses.

Effect of Korean pine nut oil on hepatic iron, copper, and zinc status and expression of genes and proteins related to iron absorption in diet-induced obese mice

Sunhye Shin,Yeseo Lim,Jayong Chung,Soyoung Park,Sung Nim Han 한국영양학회 2021 Journal of Nutrition and Health Vol.54 No.5

Purpose: Body adiposity is negatively correlated with hepatic iron status, and Korean pine nut oil (PNO) has been reported to reduce adiposity. Therefore, we aimed to study the effects of PNO on adiposity, hepatic mineral status, and the expression of genes and proteins involved in iron absorption. Methods: Five-week-old male C57BL/6 mice were fed a control diet containing 10% kcal from PNO (PC) or soybean oil (SBO; SC), or a high-fat diet (HFD) containing 35% kcal from lard and 10% kcal from PNO (PHFD) or SBO (SHFD). Hepatic iron, copper, and zinc content; and expression of genes and proteins related to iron absorption were measured. Results: HFD-fed mice had a higher white fat mass (2-fold; p < 0.001), lower hepatic iron content (25% lower; p < 0.001), and lower hepatic Hamp (p = 0.028) and duodenal Dcytb mRNA levels (p = 0.037) compared to the control diet-fed mice. Hepatic iron status was negatively correlated with body weight (r = −0.607, p < 0.001) and white fat mass (r = −0.745, p < 0.001). Although the PHFD group gained less body weight (18% less; p < 0.05) and white fat mass (18% less; p < 0.05) than the SHFD group, the hepatic iron status impaired by the HFD feeding did not improve. The expression of hepatic and duodenal ferroportin protein was not affected by the fat amount or the oil type. PNO-fed mice had significantly lower Slc11a2 (p = 0.022) and Slc40a1 expression (p = 0.027) compared to SBO-fed mice. However, the PC group had a higher Heph expression than the SC group (p < 0.05). The hepatic copper and zinc content did not differ between the four diet groups, but hepatic copper content adjusted by body weight was significantly lower in the HFD-fed mice compared to the control diet-fed mice. Conclusion: HFD-induced obesity decreased hepatic iron storage by affecting the regulation of genes related to iron absorption; however, the 18% less white fat mass in the PHFD group was not enough to improve the iron status compared to the SHFD group. The hepatic copper and zinc status was not altered by the fat amount or the oil type.

QTNAAS: 템플릿 기반 양자화된 신경망 구조 및 가속기 탐색 프레임워크

임하영(HaYoung Lim),김경미(Kyungmi Kim),장예서(Yeseo Jang),김주연(Juyeon Kim),심재형(Jaehyeong Sim) 대한전자공학회 2023 대한전자공학회 학술대회 Vol.2023 No.11

This work presents QTNAAS, a quantized template-based neural architecture accelerator search framework. Each neural operator with various quantization levels is paired with the optimal hardware block that executes the operator efficiently. We call this pair a quantized template. This approach reduces both the search space and time consumed for performance estimation, hence make it highly probable to find optimal neural architecture. This found neural architecture is bind with hardware through templates, sustaining 100% utilization. Evaluation results show that our method can build a neural network with comparable accuracy even employing mixed-precision and an accelerator with lower latency and energy consumption compared to the existing works.

Dietary supplementation with Korean pine nut oil decreases body fat accumulation and dysregulation of the appetite-suppressing pathway in the hypothalamus of high-fat diet-induced obese mice

Sunhye Shin,Soyoung Park,Yeseo Lim,Sung Nim Han 한국영양학회 2022 Nutrition Research and Practice Vol.16 No.3

BACKGROUND/OBJECTIVES: Korean pine nut oil (PNO) has been reported to suppress appetite by increasing satiety hormone release. However, previous studies have rendered inconsistent results and there is lack of information on whether dietary Korean PNO affects the expression of satiety hormone receptors and hypothalamic neuropeptides. Therefore, our study sought to evaluate the chronic effects of Korean PNO on the long-term regulation of energy balance. MATERIALS/METHODS: Five-week-old male C57BL/6 mice were fed with control diets containing 10% kcal fat from Korean PNO or soybean oil (SBO) (PC or SC) or high-fat diets (HFDs) containing 35% kcal fat from lard and 10% kcal fat from Korean PNO or SBO (PHFD or SHFD) for 12 weeks. The expression of gastrointestinal satiety hormone receptors, hypothalamic neuropeptides, and genes related to intestinal lipid absorption and adipose lipid metabolism was then measured. RESULTS: There was no difference in the daily food intake between PNO- and SBO-fed mice; however, the PC and PHFD groups accumulated 30% and 18% less fat compared to SC and SHFD, respectively. Korean PNO-fed mice exhibited higher messenger RNA (mRNA) expression of Ghsr (ghrelin receptor) and Agrp (agouti-related peptide) (P < 0.05), which are expressed when energy consumption is low to induce appetite as well as the appetite-suppressing neuropeptides Pomc and Cartpt (P = 0.079 and 0.056, respectively). Korean PNO downregulated jejunal Cd36 and epididymal Lpl mRNA expressions, which could suppress intestinal fatty acid absorption and fat storage in white adipose tissue. Consistent with these findings, Korean PNO-fed mice had higher levels of fecal non-esterified fatty acid excretion. Korean PNO also tended to downregulate jejunal Apoa4 and upregulate epididymal Adrb3 mRNA levels, suggesting that PNO may decrease chylomicron synthesis and induce lipolysis. CONCLUSIONS: In summary, Korean PNO attenuated body fat accumulation, and appeared to prevent HFD-induced dysregulation of the hypothalamic appetite-suppressing pathway.

Korean pine nut oil replacement decreases intestinal lipid uptake while improves hepatic lipid metabolism in mice

Zhu, Shuang,Park, Soyoung,Lim, Yeseo,Shin, Sunhye,Han, Sung Nim The Korean Nutrition Society 2016 Nutrition Research and Practice Vol.10 No.5

BACKGROUND/OBJECTIVES: Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim of this study was to examine the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed control or HFD. MATERIALS/METHODS: Five-week-old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO, or HFD containing 15% energy fat from lard and 30% energy fat from SBO or PNO for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling (Cd36, Fatp4, Acsl5, Acbp), intestinal chylomicron synthesis (Mtp, ApoB48, ApoA4), hepatic lipid uptake and channeling (Lrp1, Fatp5, Acsl1, Acbp), hepatic triacylglycerol (TAG) lipolysis and FA oxidation (Atgl, Cpt1a, Acadl, Ehhadh, Acaa1), as well as very low-density lipoprotein (VLDL) assembly (ApoB100) were determined by real-time PCR. RESULTS: In intestine, significantly lower Cd36 mRNA expression (P<0.05) and a tendency of lower ApoA4 mRNA levels (P = 0.07) was observed in PNO-fed mice, indicating that PNO consumption may decrease intestinal FA uptake and chylomicron assembly. PNO consumption tended to result in higher hepatic mRNA levels of Atgl (P = 0.08) and Cpt1a (P = 0.05). Significantly higher hepatic mRNA levels of Acadl and ApoB100 were detected in mice fed PNO diet (P<0.05). These results suggest that PNO could increase hepatic TAG metabolism; mitochondrial fatty acid oxidation and VLDL assembly. CONCLUSIONS: PNO replacement in the diet might function in prevention of excessive lipid uptake by intestine and improve hepatic lipid metabolism in both control diet and HFD fed mice.

노인장기요양제도 내 간호관련 연구 동향과 시사점

임지영,김예서,송성숙,김성준,Lim, Ji Young,Kim, Yeseo,Song, Sung Sook,Kim, Sungjun 한국가정간호학회 2022 가정간호학회지 Vol.29 No.1

Purpose: This study was performed to explore the trend of nursing research in the Korean long-term care system. Methods: Articles published between 2008 and 2021 were searched using the terms "long-term care system" and "nursing." Ninety-one articles were analyzed and classified into five categories according to research methods. Data were extracted through a systematic review process and underwent descriptive statistics and content analyses. Results: The most analyzed variable in the survey studies was job satisfaction. Many variables were classified into input and output factors using Donabedian's model. The content analysis showed that most suggestions were about improving the political regulation system. Conclusion: It is necessary to establish a research foundation to provide research funding and support to cultivate future nursing research in long-term care. Systematic improvement of research in nursing should be continuously pursued to revitalize nursing services, expand nursing service interventions, and improve management competency programs in nursing institutions.

Korean pine nut oil replacement decreases intestinal lipid uptake while improves hepatic lipid metabolism in mice

한성림,Shuang Zhu,Soyoung Park,Yeseo Lim,Sunhye Shin 한국영양학회 2016 Nutrition Research and Practice Vol.10 No.5

BACKGROUND/OBJECTIVES: Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim of this study was to examine the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed control or HFD. MATERIALS/METHODS: Five-week-old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO, or HFD containing 15% energy fat from lard and 30% energy fat from SBO or PNO for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling (Cd36, Fatp4, Acsl5, Acbp), intestinal chylomicron synthesis (Mtp, ApoB48, ApoA4), hepatic lipid uptake and channeling (Lrp1, Fatp5, Acsl1, Acbp), hepatic triacylglycerol (TAG) lipolysis and FA oxidation (Atgl, Cpt1a, Acadl, Ehhadh, Acaa1), as well as very low-density lipoprotein (VLDL) assembly (ApoB100) were determined by real-time PCR. RESULTS: In intestine, significantly lower Cd36 mRNA expression (P<0.05) and a tendency of lower ApoA4 mRNA levels (P = 0.07) was observed in PNO-fed mice, indicating that PNO consumption may decrease intestinal FA uptake and chylomicron assembly. PNO consumption tended to result in higher hepatic mRNA levels of Atgl (P = 0.08) and Cpt1a (P = 0.05). Significantly higher hepatic mRNA levels of Acadl and ApoB100 were detected in mice fed PNO diet (P<0.05). These results suggest that PNO could increase hepatic TAG metabolism; mitochondrial fatty acid oxidation and VLDL assembly. CONCLUSIONS: PNO replacement in the diet might function in prevention of excessive lipid uptake by intestine and improve hepatic lipid metabolism in both control diet and HFD fed mice.

Recent research and development progress of physical processes in the Korean Integrated Model

Hyun-Joo Choi,Kyoungmi Cho,Jeong-Hyun Park,Jeong-Ock Lim,Bo-Reum Han,Yeseo Park,Suk-Hawn Kim,Seon-Kyun Baek,Young Cheol Kwon 한국기상학회 2022 한국기상학회 학술대회 논문집 Vol.2022 No.10