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Park, Yeonju,Seo, Yongil,Chae, Boknam,Pyo, Dongjin,Chung, Hoeil,Hwang, Hyonseok,Jung, Young Mee WILEY‐VCH Verlag 2015 ChemPhysChem Vol.16 No.2
<P><B>Abstract</B></P><P>In this study, the thermal denaturation mechanism and secondary structures of two types of human insulin nanoparticles produced by a process of solution‐enhanced dispersion by supercritical fluids using dimethyl sulfoxide (DMSO) and ethanol (EtOH) solutions of insulin are investigated using spectroscopic approaches and molecular dynamics calculations. First, the temperature‐dependent IR spectra of spherical and rod‐shaped insulin nanoparticles prepared from DMSO and EtOH solution, respectively, are analyzed using principal component analysis (PCA) and 2D correlation spectroscopy to obtain a deeper understanding of the molecular structures and thermal behavior of the two insulin particle shapes. All‐atom molecular dynamics (AAMD) calculations are performed to investigate the influence of the solvent molecules on the production of the insulin nanoparticles and to elucidate the geometric differences between the two types of nanoparticles. The results of the PCA, the 2D correlation spectroscopic analysis, and the AAMD calculations clearly reveal that the thermal denaturation mechanisms and the degrees of hydrogen bonding in the spherical and rod‐shaped insulin nanoparticles are different. The polarity of the solvent might not alter the structure or function of the insulin produced, but the solvent polarity does influence the synthesis of different shapes of insulin nanoparticles.</P>
Formation mechanism of BAMLET by 2D Raman correlation analysis
Park, Yeonju,Park, Yujeong,Jin, Sila,Kim, Jung Won,Jung, Young Mee Elsevier 2018 Journal of molecular structure Vol.1171 No.-
<P><B>Abstract</B></P> <P>A partially unfolded bovine α-lactalbumin (ALA) and oleic acid (OA) complex called bovine α-lactalbumin made lethal to tumor cells (BAMLET) is a tumoricidal complex. For the first time, this study investigated the pH-induced formation mechanism of the ALA/OA complex using Raman spectroscopy. For a deeper understanding of the formation mechanism of the ALA/OA complex with decreasing pH, principal component analysis (PCA) and two-dimensional correlation spectroscopy (2D-COS) were applied to pH-dependent Raman spectra of the ALA/OA complex. Based on the results of the PCA and 2D-COS analyses, we successfully elucidated the formation mechanism of the ALA/OA complex at the molecular level. We also confirm that the ALA/OA complex formed below pH 3.0.</P> <P><B>Highlights</B></P> <P> <UL> <LI> For the first time, we investigated the pH-induced formation mechanism of the α-lactalbumin/oleic acid (ALA/OA) complex using Raman spectroscopy. </LI> <LI> We successfully elucidated the formation mechanism of the ALA/OA complex at the molecular level. </LI> <LI> Results of the PCA and 2D-COS analyses showed the ALA/OA complex formed below pH 3.0. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Frost avoidance: sodium alginate + CaCl2 can postpone flowering of ‘Kawanakajima Hakuto’ peach trees
Park Yeonju,신현석 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.5
The rise in early spring temperatures due to climate change has been advancing the flowering period of peach trees. This phenomenon increases the possibility of frost damage in early spring and consequently reduces the productivity of peach trees, causing economic losses. Under aberrant spring temperatures, delaying peach flowering could be used to avoid frost damage. Thus, the objective of this study was to investigate whether treatment with sodium alginate and CaCl2 in the 2nd (swollen bud) blooming stage could delay peach flowering as a strategy to avoid frost. Regarding the expression rate at the 3rd (calyx green) stage, the control treatment (CT, distilled water) showed a sharp increase from 8 days after treatment (DAT), peaking at 14 DAT. However, the 5AG group (5% sodium alginate + 100 mM CaCl2) and the 7AG group (7% sodium alginate + 100 mM CaCl2) had the maximum expression rate at 17 DAT. After maximum expression, expression in the CT sharply diminished, whereas the 5AG and 7AG treatments showed gentle decreases. At the 4th (calyx red) stage, the CT, 5AG, and 7AG treatments reached peak expression at 20 DAT (43.5%, 31.9%, and 40.2%, respectively). How- ever, maximum expression levels of all treatments were below ~ 50%. The 5th (first pink) stage was first expressed in all treatments when the expression rate at the 4th stage peaked in all groups at 20 DAT. Maximum expression was 50.7% in the CT, 33.7% in the 5AG treatment, and 49.2% in the 7AG treatment. This tendency was similar to that at the 4th stage. Particularly, expression in the 5AG treatment diminished more slowly than that in the CT and 7AG treatment at the 3rd, 4th, and 5th stages. The CT group bloomed first at 20 DAT, reaching full bloom at 81.4%, ahead of the AG treatments at 28 DAT, while the 7AG and 5AG groups bloomed fully at 34 and 37 DAT, respectively. Our results suggest the 5AG treatment is suitable to delay peach flowering when phenological development of the flower buds reaches the 2nd stage.
박연주(Park, Yeonju) 한국사회복지학회 2014 한국사회복지학 Vol.66 No.2
본 연구의 목적은 아동권리보호를 위한 아동학대 관련 판례를 분석하는 것이다. 법에서 ‘아동학대’라 칭하여 판례가 나오기 위해서는 처벌 근거인 ‘아동 학대처벌’ 관련법이 있어야 하는데, 기본법인 아동 복지법에서의 ‘아동학대’개념만 있어 처벌을 위한 직접적 판시로는 친권상실관련 ‘아동학대’ 판례와 형 사범죄, 민사범죄, 특례법 판시만 내려지고 있어 ‘아동 학대범죄 처벌에 관한 특례법’이 절실하였다(작 년 12월 23일 아동학대범죄처벌에 관한 특례법이 국회를 통과하였다). 이에 본 연구를 통해 재판상 아동학대라 판시하지는 않았으나, 아동학대로 볼 수 있는 2000-2013년 판례를 묶어 판례분석을 하 였다. 본 연구에서는 각 판례들을 사실관계에 따른 분석, 판시내용에 따른 분석을 함에 대법원판례, 대법원에 올라간 판시를 제외한 하급심종결판례를 구분하면서 민사상 합의된 건은 제외하고 불법행위 로 성립, 형사사건화 되지 않고 민사사건으로 종결된 민사상판례분석, 형사상판례분석, 친권상실(아동 학대와 관련하여)판례구분, 그 밖의 특례법상의 판례를 구분하여 도식화 진단하여 각 판례의 시사점을 통해 현 법제가 갖고 있는 문제점을 도출하고 그에 대한 입법 상 보완과제를 제시하면서 아동학대범죄 처벌에 관한 특례법상의 보완과제를 제시하였다. The purpose of this study is to analyze precedents related with child abuse for protection of the rights of the child. There should be the law related with 'punishment for child abuse,' which is the grounds of punishment, to make a precedent in the law punishing for 'child abuse,' but there is the concept only for 'child abuse' in the Child Welfare Law, the fundamental law; therefore, for a direct judgment for punishment, only precedents of 'child abuse' related with loss of parental rights and judgements for criminal cases, civil cases and laws covering special cases have been made. For that reason, 'the special law related with punishment for child abuse cases' is desperately required (On last December 23, 2013, the special law related with punishment for child abuse cases passed the National Assembly). Hence, precedent analysis had performed by grouping precedent from 2000 to 2013 which were not judged as child abuse in trial but can be regarded as child abuse. When analyzing each precedent according to the contents of analysis and judgment by fact relevance in this study, problems which the current legislative system has were deducted through an implication of each case by diagnosing using diagraming after classifying lower instance terminated cases, which precedents of the Supreme Court and judgments sent to the Supreme Court were excluded, while excluding cases settled in the civil level and classifying analysis of civil case precedents which did not become a criminal case and completed as a civil case, analysis of criminal case precedents, classification of precedents of loss of the parental rights (regarding child abuse) and precedents of any other special laws. And compensatory tasks for special laws regarding punishment of child abuse were presented while suggesting compensatory tasks for the legislation regarding deducted problems.
Nuri Park,Euidong Hwang,Yeonju Kim,Yeongju Park,강덕훈,Jonghoon Kim,홍익선,Gyeongbok Jo,송호섭,민경욱,YU YI 한국천문학회 2021 Journal of The Korean Astronomical Society Vol.54 No.1
The Ozone Dynamics Investigation Nano-Satellite (ODIN) is a CubeSat design proposed by Chungnam National University as contribution to the CubeSat Competition 2019 sponsored by the Korean Aerospace Research Institute (KARI). The main objectives of ODIN are (1) to observe the polar ozone column density (latitude range of 60° to 80° in both hemispheres) and (2) to investigate the chemical dynamics between stratospheric ozone and ozone depleting substances (ODSs) through spectroscopy of the terrestrial atmosphere. For the operation of ODIN, a highly efficient power system designed for the specific orbit is required. We present the conceptual structural design of ODIN and an analysis of power generation in a sun synchronous orbit (SSO) using two different configurations of 3U solar panels (a deployed model and a non-deployed model). The deployed solar panel model generates 189.7 W through one day which consists of 14 orbit cycles, while the non-deployed solar panel model generates 152.6 W. Both models generate enough power for ODIN and the calculation suggests that the deployed solar panel model can generate slightly more power than the non-deployed solar panel model in a single orbit cycle. We eventually selected the non-deployed solar panel model for our design because of its robustness against vibration during the launch sequence and the capability of stable power generation through a whole day cycle.