Removal of Pb2+ from the aqueous solution by tartrate intercalated layered double hydroxides

Yanming Shen,Xiaolei Zhao,Xi Zhang,Shifeng Li,Dongbin Liu,Lihui Fan 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.1

Adsorption of Pb2+ ion by a tartrate intercalated MgAl layered double hydroxides (MgAl-TA LDHs) was studied. The adsorption isotherms and kinetics were investigated as a function of various experimental parameters using batch adsorption experiments. The results indicated that the adsorption isotherm was well described by Sips model. The kinetic adsorption data were fitted well to the pseudo-second-order kinetic equation. The adsorption of Pb2+ was controlled mainly by the chemical process combined with intraparticle diffusion. Parameters of adsorption thermodynamic suggested that the interaction of Pb2+ adsorbed by MgAl-TA LDHs adsorbents was thermodynamically spontaneous and endothermic.

Cloning and sequence analysis of Wild Argali short palate, lung and nasal epithelium clone 1 cDNA

Wen Shen,Kaili Chen,Yanming Sun,Haiying Guo,Dongmei Chen,Yang Cao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.5

Objective: Experiments were conducted to clone the sequence of Wild Argali short palate, lung and nasal epithelium clone 1 (SPLUNC1) cDNA, and to lay the foundation for further study the biological function of Wild Argali SPLUNC1. Methods: The complete sequence of Wild Argali SPLUNC1 cDNA was generated by rapid amplification of cDNA ends. The entire coding sequence was inserted into the pPIC9K vector and expressed in Pichia pastoris (P. pastoris) GS115. The recombinant SPLUNC1 protein was detected by Western blot and purified by Ni2+ chelate affinity chromatography. The test of effect of the protein on Mycoplasma ovipneumoniae (MO) was performed with real-time polymerase chain reaction. Results: The Wild Argali SPLUNC1 cDNA was 1,076 bp with an open reading frame of 768 bp, which encoded a 26.49 kDa protein composed of 255 amino acids. Its amino acid sequence shared 98.4%, 96.9%, 94.5%, 90.2%, 80.8%, 78.4%, 78.3%, 72.5%, 72.3%, 68.8% identity with those of SPLUNC1 cDNA from Ovis aries (accession no. NP_001288334.1), Capra hircus (accession no. XP_005688516.1), Pantholops hodgsonii (accession no. XP_005979709.1), Bos taurus (accession no. NP_776851.1), Felis catus (accession no. XP_006929910.1), Homo sapiens (accession no. NP_001230122.1), Sus scrofa (accession no. NP_001005727.1), Chinchilla lanigera (accession no. NP_001269294.1), Mus musculus (accession no. NP_035256.2), and Rattus norvegicus (accession no. NP_742028.1), respectively. The recombinant protein corresponded to the expected molecular mass of 25.47 kDa as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was detected in the supernatant of P. pastoris, and it could be purified. The results from the test of inhibition effect of argali recombinant SPLUNC1 protein on MO showed that the product could inhibit MO very well (p<0.01). Conclusion: The amino acid sequence of Wild Argali SPLUNC1 was different from other organisms. The recombinant SPLUNC1 protein has good biological activity.

Cloning and Sequence Analysis of Wild Argali ISG15 cDNA

Sun, Yanming,Chen, Kaili,Shen, Wen,Cui, Rupeng,Lu, Haifu Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4

The complete coding sequence of Wild Argali ISG15 cDNA was generated by rapid amplification of cDNA ends. The ISG15 cDNA was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kDa protein composed of 157 amino acids. Its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of ISG15cDNA from Ovis aries (accession no. NM001009735.1), Capra hircus (accession no. HQ329186.1), Bos taurus (accession no. BC102318.1), Bubalus bubalis (accession no. HM543269.1), and Sus scrofa (accession no. EU647216.1), respectively. The entire coding sequence was inserted into the pET-28a vector and expressed in E. coli. The recombinant protein corresponded to the expected molecular mass of 25 kDa as judged by SDS-PAGE, and it was detected in the bacterial inclusion bodies. The expressed protein could be purified by $Ni^{2+}$ chelate affinity chromatography and the results from the lymphocyte proliferation test showed that the product could stimulate lymphocyte proliferation very well (p<0.05), which further confirmed its biological activity.

Encapsulation of 2,4-Dihydroxybenzophenone into Dodecylbenzenesulfonate Modified Layered Double Hydroxide for UV Absorption Properties

Shifeng Li,Yanming Shen,Dongbin Liu,Lihui Fan,Keke Wu 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.2

New organic-inorganic composite of 2,4-dihydroxybenzophenone (BP-1) encapsulation into dodecylbenzenesulfonate (DBS) modified layered double hydroxide (LDH) was successfully prepared. The surface, structural, thermal and absorption properties of the BP-1/DBS-LDH nanohybrid was characterized by BET analysis, scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG) and diffuse reflectance UV-Vis absorbance spectra (DRUV-vis). The interlayer configuration of composite and the adsorption mechanism of BP-1 on MgAl-DBS-LDH were discussed. It was suspected that DBS anions located in the form of monolayer arrangement with a 75° anti parallel angle between dodecylbenzenesulfonate chain axis. The diffuse reflectance UV-Vis absorbance results revealed that the UV absorbing wavelength of BP-1/DBS-LDH evidently extends to about 400 nm, which shows that the BP-1/DBS-LDH has the potential application as a UV absorber.

Encapsulation of 2,4-Dihydroxybenzophenone into Dodecylbenzenesulfonate Modified Layered Double Hydroxide for UV Absorption Properties

Li, Shifeng,Shen, Yanming,Liu, Dongbin,Fan, Lihui,Wu, Keke Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.2

New organic-inorganic composite of 2,4-dihydroxybenzophenone (BP-1) encapsulation into dodecylbenzenesulfonate (DBS) modified layered double hydroxide (LDH) was successfully prepared. The surface, structural, thermal and absorption properties of the BP-1/DBS-LDH nanohybrid was characterized by BET analysis, scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TG) and diffuse reflectance UV-Vis absorbance spectra (DRUV-vis). The interlayer configuration of composite and the adsorption mechanism of BP-1 on MgAl-DBS-LDH were discussed. It was suspected that DBS anions located in the form of monolayer arrangement with a $75^{\circ}$ anti parallel angle between dodecylbenzenesulfonate chain axis. The diffuse reflectance UV-Vis absorbance results revealed that the UV absorbing wavelength of BP-1/DBS-LDH evidently extends to about 400 nm, which shows that the BP-1/DBS-LDH has the potential application as a UV absorber.

Intercalation of 2,4-dihydroxybenzophenone-5-sulfonate anion into Zn/Al layered double hydroxides for UV absorption properties

Shifeng Li,Yanming Shen,Min Xiao,Dongbin Liu,Lihui Fan,Keke Wu 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4

Zn/Al-layered double hydroxides (LDHs) containing the organic anion of 2,4-dihydroxybenzophenone-5-sulfonate (DHBS) have been synthesized by co-precipitation method. Surface, structural, thermal and absorption properties of the ZnAl-DHBS-LDHs nanohybrids were characterized by BET analysis, scanning electron microscope, X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetry and differential thermal analysis and UV–visible spectroscopy. The results revealed that organic UV absorbent-DHBS has been intercalated into the interlayer spaces of ZnAl-LDHs and the intercalation of DHBS in LDHs interlayer galleries resulted to higher thermal stability and better UV absorption ability; these show that the hybrid ZnAl-DHBS-LDHs phase has potential applications as a UV absorber.

Job-aware Network Scheduling for Hadoop Cluster

( Wen Liu ),( Zhigang Wang ),( Yanming Shen ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.1

In recent years, data centers have become the core infrastructure to deal with big data processing. For these big data applications, network transmission has become one of the most important factors affecting the performance. In order to improve network utilization and reduce job completion time, in this paper, by real-time monitoring from the application layer, we propose job-aware priority scheduling. Our approach takes the correlations of flows in the same job into account, and flows in the same job are assigned the same priority. Therefore, we expect that flows in the same job finish their transmissions at about the same time, avoiding lagging flows. To achieve load balancing, two approaches (Flow-based and Spray) using ECMP (Equal-Cost multi-path routing) are presented. We implemented our scheme using NS-2 simulator. In our evaluations, we emulate real network environment by setting background traffic, scheduling delay and link failures. The experimental results show that our approach can enhance the Hadoop job execution efficiency of the shuffle stage, significantly reduce the network transmission time of the highest priority job.

Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean

Zhou, Zhengkui,Jiang, Yu,Wang, Zheng,Gou, Zhiheng,Lyu, Jun,Li, Weiyu,Yu, Yanjun,Shu, Liping,Zhao, Yingjun,Ma, Yanming,Fang, Chao,Shen, Yanting,Liu, Tengfei,Li, Congcong,Li, Qing,Wu, Mian,Wang, Min,Wu, Nature Publishing Group, a division of Macmillan P 2015 Nature biotechnology Vol.33 No.4

Understanding soybean (Glycine max) domestication and improvement at a genetic level is important to inform future efforts to further improve a crop that provides the world's main source of oilseed. We detect 230 selective sweeps and 162 selected copy number variants by analysis of 302 resequenced wild, landrace and improved soybean accessions at >11× depth. A genome-wide association study using these new sequences reveals associations between 10 selected regions and 9 domestication or improvement traits, and identifies 13 previously uncharacterized loci for agronomic traits including oil content, plant height and pubescence form. Combined with previous quantitative trait loci (QTL) information, we find that, of the 230 selected regions, 96 correlate with reported oil QTLs and 21 contain fatty acid biosynthesis genes. Moreover, we observe that some traits and loci are associated with geographical regions, which shows that soybean populations are structured geographically. This study provides resources for genomics-enabled improvements in soybean breeding.