라텍스 장갑의 세척 방법에 따른 세균 감소율 측정

양송이 ( Song Yi Yang ),오정민 ( Jeong Min Oh ),송다혜 ( Da Hye Song ),송보람 ( Bo Ram Song ),강명진 ( Myung Jin Kang ),이명선 ( Myung Sun Lee ),손가연 ( Ga Yeon Son ),오상환 ( Sang Hwan Oh ) 한국치위생학회(구 한국치위생교육학회) 2014 한국치위생학회지 Vol.14 No.4

Objectives : Latex gloves hygiene is the most effective method to prevent infection of microorganisms and to reduce the incidence of cross infections. The aim of this study was to compare the bacteria reduction ratio of cleansing with water, liquid soap and alcohol gauze. Methods : The left side glove was the control group and the right side was the experimental group. The experimental group washed hand with water, soap, and alcohol gauze. The hand plate was inoculated by the hand and inoculated for 24 hours in 35℃. Results : Washing with water showed that CFU of control group was 1116.9 and that of experimental group was 302.8. Hand washing by water reduced 74.3% of bacteria. Liquid soap revealed that CFU of control group was 619.9 and that of experimental group was 8.3. Hand washing by liquid soap reduced 97.5% of bacteria. Alcohol gauze included 875.2 CFU in control group and 5.8 CFU in experimental group. Washing by alcohol gauze reduced 99.5% of bacteria. Conclusions : Based on the results, the most effective latex gloves cleansing method was recommended as the standardized hand washing with the liquid hand soap and alcohol gauze. The results can be used to improve training strategies for enhancing glove hygiene practice in dental clinic.

A MALDI-MS-based Glucan Hydrolase Assay Method for Whole-cell Biocatalysis

( Da-hee Ahn ),( Han-gyu Park ),( Won-suk Song ),( Seong-min Kim ),( Sung-hyun Jo ),( Yung-hun Yang ),( Yun-gon Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 한국미생물·생명공학회지 Vol.47 No.1

Screening microorganisms that can produce glucan hydrolases for industrial, environmental, and biomedical applications is important. Herein, we describe a novel approach to perform glucan hydrolase screening―based on analysis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) spectra―which involves degradation of the oligo- and polysaccharides. As a proof-of-concept study, glucan hydrolases that could break down glucans made of several glucose units were used to demonstrate the MALDI-MS-based enzyme assay. First, the enzyme activities of α-amylase and cellulase on a mixture of glucan oligosaccharides were successfully discriminated, where changes of the MALDI-MS profiles directly reflected the glucan hydrolase activities. Next, we validated that this MALDI-MS-based enzyme assay could be applied to glucan polysaccharides (i.e., pullulan, lichenan, and schizophyllan). Finally, the bacterial glucan hydrolase activities were screened on 96-well plate-based platforms, using cell lysates or samples of secreted enzyme. Our results demonstrated that the MALDI-MS-based enzyme assay system would be useful for investigating bacterial glucoside hydrolases in a high-throughput manner.

Sesquiterpenes from fruits of Torilis japonica with inhibitory activity on melanin synthesis in B16 cells

Song, Da Hye,Jo, Yang Hee,Ahn, Jong Hoon,Kim, Seon Beom,Yun, Cheong-Yong,Kim, Youngsoo,Hwang, Bang Yeon,Lee, Mi Kyeong Springer-Verlag 2018 Journal of natural medicines Vol.72 No.1

<P>Melanin, a dark macromolecular pigment, protects skin from harmful damage. However, abnormal accumulation is responsible for hyperpigmentation disorders. Melanogenesis inhibitors have therefore become important constituents in cosmetic products for depigmentation. Torilis japonica Decandolle (Umbelliferae) is a biennial plant which is distributed in East Asia. Fruits of this plant have been used for the treatment of skin disease and inflammation. In our previous study, torilin, a major sesquiterpene of T. japonica, showed an inhibitory effect on melanin production in alpha-melanocyte stimulating hormone (alpha-MSH)-activated B16 melanoma cells. Further extensive chromatographic separation resulted in thirteen compounds. On the basis of spectroscopic analysis, the structures of the compounds isolated were determined to be three new sesquiterpenes, viz. a guaiane-type, epoxytorilinol (1), a eudesmane-type, elematorilone (2) and a cadinane-type, cardinatoriloside (3), together with ten known sesquiterpenes (4-13). Of the compounds isolated, compounds 4-6 and 11-13 inhibited alpha-MSH-activated melanin production in B16 melanoma cells with IC50 values from 72.9 to 191.0 mu M.</P>

A MALDI-MS-based Glucan Hydrolase Assay Method for Whole-cell Biocatalysis

Ahn, Da-Hee,Park, Han-Gyu,Song, Won-Suk,Kim, Seong-Min,Jo, Sung-Hyun,Yang, Yung-Hun,Kim, Yun-Gon The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.1

Screening microorganisms that can produce glucan hydrolases for industrial, environmental, and biomedical applications is important. Herein, we describe a novel approach to perform glucan hydrolase screening-based on analysis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) spectra-which involves degradation of the oligo- and polysaccharides. As a proof-of-concept study, glucan hydrolases that could break down glucans made of several glucose units were used to demonstrate the MALDI-MS-based enzyme assay. First, the enzyme activities of ${\alpha}$-amylase and cellulase on a mixture of glucan oligosaccharides were successfully discriminated, where changes of the MALDI-MS profiles directly reflected the glucan hydrolase activities. Next, we validated that this MALDI-MS-based enzyme assay could be applied to glucan polysaccharides (i.e., pullulan, lichenan, and schizophyllan). Finally, the bacterial glucan hydrolase activities were screened on 96-well plate-based platforms, using cell lysates or samples of secreted enzyme. Our results demonstrated that the MALDI-MS-based enzyme assay system would be useful for investigating bacterial glucoside hydrolases in a high-throughput manner.

Hydrogen sulfide alleviates hypothyroidism-induced myocardial fibrosis in rats through stimulating autophagy and inhibiting TGF-β1/Smad2 pathway

Xiong Song,Liangui Nie,Junrong Long,Junxiong Zhao,Xing Liu,Liuyang Wang,Da Liu,Sen Wang,Shengquan Liu,Jun Yang 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.1

Hypothyroidism alone can lead to myocardial fibrosis and result in heart failure, but traditional hormone replacement therapy does not improve the fibrotic situation. Hydrogen sulfide (H2S), a new gas signaling molecule, possesses antiinflammatory, antioxidant, and anti-fibrotic capabilities. Whether H2S could improve hypothyroidism-induced myocardial fibrosis are not yet studied. In our study, H2S could decrease collagen deposition in the myocardial tissue of rats caused by hypothyroidism. Furthermore, in hypothyroidism-induced rats, we found that H2S could enhance cystathionine-gamma-lyase (CSE), not cystathionine β-synthase (CBS), protein expressions. Finally, we noticed that H2S could elevate autophagy levels and inhibit the transforming growth factor-β1 (TGF-β1) signal transduction pathway. In conclusion, our experiments not only suggest that H2S could alleviate hypothyroidism- induced myocardial fibrosis by activating autophagy and suppressing TGF-β1/ SMAD family member 2 (Smad 2) signal transduction pathway, but also show that it can be used as a complementary treatment to conventional hormone therapy.

Overexpression of Hiwi Promotes Growth of Human Breast Cancer Cells

Wang, Da-Wei,Wang, Zhao-Hui,Wang, Ling-Ling,Song, Yang,Zhang, Gui-Zhen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

The Piwi subfamily comprises two argonaute (Ago) family proteins, which are defined by the presence of PAZ and Piwi domains, with well known roles in RNA silencing. Hiwi, a human Piwi subfamily member, has been shown to play essential roles in stem cell self-renewal and gametogenesis. Recently, accumulating reports have indicated that abnormal hiwi expression is associated with poorer prognosis of multiple types of human cancers, including examples in the breast. However, little is known about details of the oncogenic role of hiwi in breast cancers. In present study, we confirmed overexpression of hiwi in breast cancer specimens and breast cancer cell lines at both mRNA and protein levels. Thus both RT-qPCR and Western blot data revealed significantly higher hiwi in intratumor than peritumor specimens, overexpression being associated with tumor size, lymph node metastasis and histological grade. Hiwi overexpression was also identified in breast cancer cell lines, MDA-MB-231 and MCF-7, and gain-of-function and loss-of-function strategies were adopted to identify the role of hiwi in the MCF-7 cell growth. Results demonstrated that hiwi expression in MCF-7 cells was significantly up- or down-regulated by the two strategies. We next evaluated the influence of hiwi overexpression or knockdown on the growth of breast cancer cells. Both cell count and colony formation assays confirmed promoting roles of hiwi in MCF-7 cells, which could be inhibited by hiwi specific blockage by siRNAs. In summary, the present study confirmed overexpression of hiwi in breast cancer specimens and breast cancer cell lines, and provided e vidence of promotion by hiwi of cell growth. The results imply an oncogenic role of hiwi in breast cancers.

MicroRNA-200 family members and ZEB2 are associated with brain metastasis in gastric adenocarcinoma

MINN, YANG-KI,LEE, DA HYE,HYUNG, WOO JIN,KIM, JI EUN,CHOI, JUNJEONG,YANG, SEUNG-HO,SONG, HOTAEK,LIM, BEOM JIN,KIM, SE HOON Spandidos Publications 2014 International journal of oncology Vol.45 No.6

<P>Although the incidence of brain metastasis in gastric cancer is relatively low, its prevalence may increase with improved therapy and longer survival tumors. The molecular mechanisms underlying brain metastases are not well understood. To gain insight into the mechanism of brain metastasis, we studied differences in microRNA (miRNA) expression levels in 8 cases of matched primary gastric adenocarcinoma and brain metastatic adenocarcinoma using the Illumina microRNA microarray chip. We identified 6 upregulated and 2 downregulated miRNAs in all 8 cases simultaneously. Interestingly, 2 out of 8 miRNAs (hsa-miR?141-3p and hsa-miR-200b-3p) belonged to the miR-200 family. Online microRNA database searching revealed that ZEB2 is the top-ranked target gene for hsa-miR141-3p and hsa-miR-200b-3p, prompting us to focus ZEB2 expression in brain metastatic adenocarcinoma. We confirmed that ZEB2 expression was markedly downregulated in some brain metastatic samples. In addition, decreased ZEB2 expression was noted by western blot analysis of 2 metastatic gastric adenocarcinoma cell types that were derived by in vivo selection following intracardiac injection of gastric cancer cell lines. In conclusion, we demonstrate that expression of miRNA-200 family members and ZEB2 are associated with brain metastases of gastric adenocarcinoma, not only in matched patient samples, but also in metastatic cell lines that were derived by in vivo selection.</P>

Facile, air-insensitive solvothermal synthesis of emission-tunable CuInS₂/ZnS quantum dots with high quantum yields

Nam, Da-Eun,Song, Woo-Seuk,Yang, Heesun Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.45

<P>Yellow-to-red emitting CuInS<SUB>2</SUB>/ZnS (CIS/ZnS) core/shell quantum dots (QDs) with a maximum photoluminescence quantum yield (QY) of ∼65% were facilely synthesized <I>via</I> a stepwise, consecutive solvothermal approach. The size-sorting experiments on CIS/ZnS QDs indicated that the ZnS shell was formed with a relatively uniform thickness even though the size of pre-existing CIS core QDs was not monodisperse. CIS core QDs solvothermally grown for different reaction times of 5–6 h exhibited poor QYs of <8.8% with deep red emissions as a result of donor–acceptor pair (DAP) recombination. Upon shell overcoating, the band gap energies of CIS/ZnS QDs increased by 0.14–0.23 eV, depending on the CIS core growth time. Such increased band gaps were attributed presumably to the reduction of actual CIS core size, originating from the formation of the alloyed interfacial layer at CIS/ZnS during a long shell growth. Compared to CIS core QDs, the emission of CIS/ZnS QDs was markedly blue-shifted by 0.10–0.18 eV. This blue-shift was discussed based on the shift of QD band gap-dependent donor/acceptor energy levels and the donor–acceptor distance-dependent DAP recombination process.</P> <P>Graphic Abstract</P><P>Highly fluorescent, emission-tunable CuInS<SUB>2</SUB>/ZnS core/shell quantum dots were synthesized by a facile, air-insensitive solvothermal route. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1jm12437d'> </P>

Associated Factors and Prevention of Upper Pole Rippling in Prepectoral Direct-to-Implant Breast Reconstruction

Ryu Da Hye,Joo Oh Young,Roh Yun Ho,Yang Eun Jung,Song Seung Yong,Lee Dong Won 대한성형외과학회 2023 Archives of Plastic Surgery Vol.50 No.6

Background Despite its many advantages, prepectoral breast reconstruction also carries the risk of implant rippling. The recent introduction of partial superior implant coverage using a pectoralis muscle slip in prepectoral direct-to-implant (DTI) breast reconstruction has shown the potential to minimize upper pole rippling. The purpose of this study was to identify factors associated with rippling and the effectiveness of our surgical technique.Methods In total, 156 patients (186 breasts) who underwent prepectoral DTI breast reconstruction between August 2019 and March 2021 were identified retrospectively. Patient data were analyzed from medical records. Univariable and multivariable logistic analyses were performed to contextualize the risks associated with rippling deformity relative to demographic characteristics and other clinical factors. Retrospective propensity-matched analysis was performed to identify the relationship between rippling deformity and the reconstruction method.Results Patients with body mass index (BMI; odds ratio [OR], 0.736; p < 0.001), those with a postoperative chemotherapy history (OR, 0.324; p = 0.027) and those who received breast reconstruction via the superior coverage technique (OR, 0.2; p = 0.004), were less likely to develop rippling deformity. The median follow-up period was 64.9 weeks, and there were no significant differences between patients in types of mastectomy, implant, or acellular dermal matrix. Patients who underwent superior coverage technique-based reconstruction showed significantly reduced rippling (OR, 0.083; p = 0.017)Conclusion Patients with higher BMI and prior postoperative chemotherapy were less likely to develop rippling deformity. The superior coverage technique can be effective in minimizing upper pole rippling.

Are Serum Vitamin D Levels Associated With Dry Eye Disease? Results From the Study Group for Environmental Eye Disease

Jeon, Da-Hye,Yeom, Hyungseon,Yang, Jaewon,Song, Jong Suk,Lee, Hyung Keun,Kim, Hyeon Chang The Korean Society for Preventive Medicine 2017 예방의학회지 Vol.50 No.6

Objectives: Dry eye disease (DED) is an increasingly important public health problem in Korea. Previous studies conducted in Korea have reported inconsistent results regarding the protective effects of vitamin D on DED, and these discrepancies may be related to the relatively simple questionnaire that has been used. Thus, we evaluated the association of serum vitamin D levels with DED using the ocular surface disease index (OSDI). Methods: The present study evaluated data from participants in the Study Group for Environmental Eye Disease (2014-2015). This group included data from 752 participants, and data from 740 participants (253 men and 487 women) were analyzed in the present study. DED severity was evaluated using the OSDI. Results: Higher serum vitamin D levels were associated with a non-significantly reduced risk of DED in the crude analysis (odds ratio [OR], 0.991; 95% confidence interval [CI], 0.971 to 1.011) and in the adjusted analysis (OR, 0.988; 95% CI, 0.966 to 1.010). In the crude analysis of no/mild DED vs. moderate/severe DED, men exhibited a decreased risk with increasing serum vitamin D levels (OR, 0.999; 95% CI, 0.950 to 1.051), while women exhibited an increased risk (OR, 1.003; 95% CI, 0.979 to 1.027). In these analyses, we found no significant associations. Conclusions: The findings of the present study support previous reports that serum vitamin D levels are not associated with DED.