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유미연,이원문,홍인기 경희-다반 ASIC 설계교육센터 2002 경희-다반 ASIC센터 논문집 Vol.3 No.-
본 논문에서는 4G 이동통신 시스템 기반 기술인 MIMO (Multi-Input Multi-Output) 시스템에서, 채널 정보를 이용할 때, 결합 송수신 최적화 형태를 유도 하였다. Lagrange Multiplier Method를 이용하여 일반적인 SINR(Signal to interference and noise ratio)의 극대값을 유도하는 과정에서, 결합 송수신 형태를 수식적으로 유도하였고, 이를 ITU-R M.1225 채널 모델에 적용하여 시뮬레이션 하였다 또한 채널에 대한 정보가 없을때, 시공간 다이버시티를 얻을 수 있는 STBC(Space time Block Coding)의 MIMO 시스템과 비교하였다.
한국전통 식품의 원료인 메주와 누룩에서 분리된 접합균에 대한 연구
유기원,성창근,이상선,유진영 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-
The fungal isolates of Mucorales, directly collected from Korean traditional raw materials of Nuruk (raw material for Korean rice wine) and Meju (raw material for Korean soysauces), were compared with those of Rhizopus oryzae purchased. The fungal isolates of Rhizopus, Mucor, and Absidia mostly identified as based on the morphological observations, were evaluated with the PCR-polymorphic bands. The PCR-polymorphic bands of the genomic DNA reacted with the primers of OPD series tenmer were various, but showed averaged 4 to 6 in the agarose-electrophoresis. The dissimilarity coefficient (DC) between two isolates were compared by the cluster analysis, dendrogams and polar ordinations. The isolates of R. oryzae known showed several groupings within the lower value of DC and were divided to two groups of amylo-process and other fungi with other purposes. The isolates unidentified were identified by the DC made of this results. Taxonomy of these isolates made by the morphological observations were consistent with those resulted above in most case but not in all aspects. More works were needed with the isolates known for detail informations of Mucorales.
당원, 삼투압 및 무기염 농도가 Panax ginseng 모상근의 생장과 Ginsenosides 생산성에 미치는 영향
한영현,임종훈,백기엽,유기원 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1998 연구보고서 Vol.3 No.-
인삼 형질전환체는 호르몬이 포함되지 않은 3/4SH 배지에 공구유출식 콘형 생물반응기를 이용하여 배양하였다. 초기 접종 량은 30g/3L로 하였으며 4-5일 간격으로 배양액 내의 당원, 전기 전도도와 pH의 변화를 측정하였다. 처음 pH는 5.8이었으나 배양8일 후 4.95까지 떨어졌으나, 이후 5.63까지 증가하였다. 전기전도도와 탄소원으로 제공된 sucrose는 일정하게 감소되었고, 특히 배양 미삼의 탄소원으로 제공된 sucrose는 배양 20일 후에 완전히 소모되었다. 분해산물인 glucose와 frutose는 20일 까지 점진적으로 증가되었고 이후 급격히 감소되었다. 400ml 플라스크 안에 100ml의 배지량을 첨가하고 3g의 배양 미삼을 접종하여 배양하였을 때 배양 미삼의 생장률은 탄소원의 농도에 따라 증가되었다. 이는 배양 미삼의 환원당, 전당, 그리고 전분의 저장함량이 증가되었다. 그러나 ginsenosides의 함량은 반 비례관계로 감소되었다. 배양 주간별 ginsenosides의 함량변화는 배양초기인 1,2 주에는 급격히 감소되었으며 3주에는 약간 증가하고 4,5주 사이에 급격히 증가되었다. 삼투조절제로 사용한 0.1, 0.2, 0.3M의 mannitol과 sobitol은 생장 면에서 0.1M의 실험구가 좋았으나 ginsenosides의 함량은 삼투압농도와 반비례 관계로 급격히 감소되었다. Panax ginseng roots transformed by Agrobacterium rhizogenes were cultured in the air-lifted cone type bubble bioreactor that contained a hormone-free SH medium with 3% sucrose. The inoculating vol. was 30g/3L. The pH, EC, glucose, fructose and sucrose were investigated every 4 or 5 days. The pH was 5.8 initially and become 4.95 after 8 days and then increased after to 5.63. EC and sucrose contents decreased with culture days and then especially sucrose, which is carbon source for bio-ginseng rots, were depleted after 20 days. Fructose and glucose content increased gradually till 20 days with regular intervals, and then decreased quickly. While the roots were culture in 400ml flask, the higher sucrose consents, the higher growth rate. The growth rate and starch, reducing and total sugar contents were increased with the increase of sucrose concenturations but the contents of ginsenoside decrease. Variations of ginsenosides each weeks decreased first 1 or 2 weeks, increase a little after 3 weeks and largely between 4 and 5 weeks of flask culture. The osmotic regulation regents on growth were treated the 0.1, 0.3, 0.5M sobitol and mannitol. Amount of the growth were all increased on 0.1M but largely decreased the ginsenoside.
Growth pattern and ginsenoside production of auxininduced Panax ginseng rooots
YU Kee-Won(유기원),KIM Yun-Soo(김윤수),KIM Mee Hee(김미희),CHA Ahn Hee(차안희),JEONG Jae Woon(정재운),LEE Yong Wook(이용욱),Yun Seung Rho(윤승로),Choi Sung-Mee(최성미),Park Hee-Ju(박희주),Lee Sang-Jong(이상종),Son Sung-Ho(손성호),PAE 한국원예학회 1999 원예과학기술지 Vol.17 No.2
Production of Adventitious Ginseng Roots Using Biorectors
Yu, Kee-Won,Hahn, Eun-Joo,Paek, Kee-Yoeup The Korean Society of Plant Biotechnology 2000 식물생명공학회지 Vol.27 No.4
Panax ginseng is an important medicinal plant that has been used worldwide for geriatric, tonic, stomachic, and aphrodisiac treatments. Ginsenosides contained in the ginseng root are the main substances having active functions for human body. The price of ginseng is very expensive due to a complex process of cultivation, and the yield of ginseng is limited, which cannot meet the demand of the increasing market. Researchers have applied plant biotechnology to solve the problems but there are still things to be determined towards ginsenoside production by large-scale adventitious root culture. In this experiment, 5 to 20 liter bioreactors were employed to determine optimal conditions for adventitious root culture and ginsenoside production of Panax gineng. Callus was induced from the ginseng root on MS agar medium containing 1.0 mg. $L^{-1}$ 2,4-D and 0.1 mg. $L^{-1}$ kinetin. Then the callus was cultured on MS agar medium supplemented with 2.0 mg. $L^{-1}$ IBA, 0.1 mg. $L^{-1}$ kinetin, and 30 g. $L^{-1}$ to induce adventitious roots. The maximum root growth and ginsenoside production were obtained in 1/2 MS medium. 2.0 mg. $L^{-1}$ naphthalene acetic acid resulted in greater root growth than 2.0 mg $L^{-1}$ indole-3-butyric acid. Ginsenoside content increased with 2.0 mg. $L^{-1}$ benzyl adenin or kinetin. High concentrations of benzyl adenin (above 3.0 mg. $L^{-1}$ ) decreased the adventitious root growth and ginsenoside productivity. N $H_{4}$$^{+}$ inhibited the ginsenoside accumulation, while high concentrations of $K^{+}$, $Mg_{2}$$^{+}$, and $Ca_{2}$$^{+}$ increased it. N $H_{4}$$^{+}$ at 0.5 and 1.0 times of the normal amount in 3/4 SH medium resulted in the greatest biomass increase, but the highest ginsenoside productivity was obtained when N $O_{3}$$^{-}$ was used as the sole nitrogen source in the medium. Most microelements at high concentrations in the medium inhibited the root growth, but high concentrations of MnS $O_4$enhanced the root growth. Root dry weight increased with increasing sucrose concentrations up to 50 g. $L^{-1}$ , but decreased from 70 g $L^{-1}$ Ginsenoside productivity was maximized at the range of 20 to 30 g. $L^{-1}$ sucrose. In the experiment on bioreactor types, cone and balloon types were determined to be favorable for both adventitious root growth and ginsenoside production. Jasmonic acid was effective for increasing ginsenoside contents and Rb group ginsenosides mainly increased. These results could be employed in commercial scale bioreactor cultures of Panax ginseng.x ginseng.