멜라민 첨가 순서에 따른 UMF 접착제의 경화거동과 접착력의 영향

( Guang-zhu Xu1 ),엄영근 ( Young Geun Eom ),이영규 ( Young Kyu Lee ),임동혁 ( Dong Hyuk Lim ),이병호 ( Byoung Ho Lee ),김현중 ( Hyun Joong Kim ) 한국 접착 및 계면학회 2009 접착 및 계면 Vol.10 No.2

Fibulin-5 is a Prognostic Marker that Contributes to Proliferation and Invasion of Human Glioma Cells

Sheng, Xu-Dong,Chen, Hu,Wang, Hui,Ding, Zhi-Bin,Xu, Gang-Zhu,Zhang, Jun-Feng,Lu, Wen-Chao,Wu, Tao,Zhao, Ling Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Fibulin-5 has recently been considered as a potential tumor suppressor in human cancers. Several studies have shown that it is down-regulated in a variety of tumor types and inhibits tumor growth and metastasis. This study was aimed to investigate the clinical significance of fibulin-5 in glioma and its role in cell proliferation and invasion. We found that the expression of fibulin-5 in glioma tissues was significantly lower than those in normal brain (NB) tissues. Negative expression was significantly correlated with advanced clinical stage (grade III+IV). Furthermore, Fibulin-5 negative expression was correlated with a shorter overall survival of glioma patients. Multivariate Cox repression analysis indicated that fibulin-5 was an independent factor for predicting overall survival of glioma patients. Overexpression obviously inhibited cell proliferation in U251 and U87 cells. Furthermore, it significantly reduced the number of migrating and invading glioma cells. In conclusion, impaired expression of fibulin-5 is correlated with the advanced tumor stage in glioma. Otherwise, Fibulin-5 is an independent prognostic marker for predicting overall survival of glioma patients. Mechanistically, it may function as a tumor suppressor via inhibiting cell proliferation and invasion in gliomas.

Characterization of an inhibitor of apoptosis gene (BmSurvivin-2) from the silkworm, Bombyx mori

Xu-Dong TANG,Qiang Tu,Fei Mao,Xing-Rong Bai,Feng Zhu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

The inhibitor of apoptosis proteins (IAP) plays an important role in maintaining the balance between cell proliferation and cell death. Here, we describe an IAP named BmSurvivin-2 isolated from tissues of Bombyx mori that contains only one baculoviral IAP repeat (BIR) domain and lacks a really interesting new gene (RING) finger domain. Cell viability and DNA fragment analysis showed that over-expression of BmSurvivin-2 could inhibit actinomycin D (ActD)-induced apoptosis in Sf9 cells, and its anti-apoptotic activity was dependent on its Nterminal BIR domain. Real-time PCR analyses indicated that BmSurvivin-2 mRNA was expressed in all tissues of silkworm and the highest expression level was found in testis and head. Expression of BmSurvivin-2 was found to gradually increase during the wandering stage of silkworm and decreased after wandering. Similar to deterin but not survivin, BmSurvivin-2 was primarily located in the cytoplasm. All these results provide insight into the role of a novel IAP in the silkworm, B. mori.

Ionic liquid-functionalized carbon dots with positive surface charge for selective detection of ascorbic acid

Xu Ouwen,Wan Shuyu,Yang Jing,Song Hanyang,Xia Ji,Dong Luzheng,Zhu Xiashi 한국탄소학회 2023 Carbon Letters Vol.33 No.2

Novel ionic liquid-functionalized carbon quantum dots (IL-CDs) were prepared by hydrothermal method, and characterized with FT-IR, TEM and XPS. The IL-CDs exhibited narrower particle size distribution with more uniform dispersion and the surface potential changes from negative to positive due to the function of IL. IL-CDs could be quenched (“turned off”) after adding ascorbic acid (AA), and as an “on–off”, fluorescent probe could be established for direct analysis AA. The linear range of AA was 0.34–30.00 μg/mL and the LOD was 0.11 μg/mL. The method was successfully applied to the determination of AA in real samples with satisfactory results.

Yaw wind effect on flutter instability of four typical bridge decks

Zhu, Le-Dong,Xu, You-Lin,Guo, Zhenshan,Chang, Guang-Zhao,Tan, Xiao Techno-Press 2013 Wind and Structures, An International Journal (WAS Vol.17 No.3

When evaluating flutter instability, it is often assumed that incident wind is normal to the longitudinal axis of a bridge and the flutter critical wind speed estimated from this direction is most unfavorable. However, the results obtained in this study via oblique sectional model tests of four typical types of bridge decks show that the lowest flutter critical wind speeds often occur in the yaw wind cases. The four types of bridge decks tested include a flat single-box deck, a flat ${\Pi}$-shaped thin-wall deck, a flat twin side-girder deck, and a truss-stiffened deck with and without a narrow central gap. The yaw wind effect could reduce the critical wind speed by about 6%, 2%, 8%, 7%, respectively, for the above four types of decks within a wind inclination angle range between $-3^{\circ}$ and $3^{\circ}$, and the yaw wind angles corresponding to the minimal critical wind speeds are between $4^{\circ}$ and $15^{\circ}$. It was also found that the flutter critical wind speed varies in an undulate manner with the increase of yaw angle, and the variation pattern is largely dependent on both deck shape and wind inclination angle. Therefore, the cosine rule based on the mean wind decomposition is generally inapplicable to the estimation of flutter critical wind speed of long-span bridges under skew winds. The unfavorable effect of yaw wind on the flutter instability of long-span bridges should be taken into consideration seriously in the future practice, especially for supper-long span bridges in strong wind regions.

Effect of 5-aza-2'-deoxycytidine on Cell Proliferation of Non-small Cell Lung Cancer Cell Line A549 Cells and Expression of the TFPI-2 Gene

Dong, Yong-Qiang,Liang, Jiang-Shui,Zhu, Shui-Bo,Zhang, Xiao-Ming,Ji, Tao,Xu, Jia-Hang,Yin, Gui-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Objective: The present study employed 5-aza-2'-deoxycytidine (5-Aza-CdR) to treat non-small cell lung cancer (NSCLC) cell line A549 to investigate the effects on proliferation and expression of the TFPI-2 gene. Methods: Proliferation was assessed by MTT assay after A549 cells were treated with 0, 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR, a specific demethylating agent, for 24, 48 and 72h. At the last time point cells were also analyzed by flow cytometry (FCM) to identify any change in their cell cycle profiles. Methylation-specific polymerase chain reaction (MSPCR), real time polymerase chain reaction(real-time PCR) and western blotting were carried out to determine TFPI-2 gene methylation status, mRNA expression and protein expression. Results: MTT assay showed that the growth of A549 cells which were treated with 5-Aza-CdR was significantly suppressed as compared with the control group (0 ${\mu}mol/L$ 5-Aza-CdR). After treatment with 0, 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR for 72h, FCM showed their proportion in G0/G1 was $69.7{\pm}0.99%$, $76.1{\pm}0.83%$, $83.8{\pm}0.35%$, $95.5{\pm}0.55%$ respectively (P<0.05), and the proportion in S was $29.8{\pm}0.43%$, $23.7{\pm}0.96%$, $15.7{\pm}0.75%$, $1.73{\pm}0.45%$, respectively (P<0.05), suggesting 5-Aza-CdR treatment induced G0/G1 phase arrest. MSPCR showed that hypermethylation in the promoter region of TFPI-2 gene was detected in control group (0 ${\mu}mol/L$ 5-Aza-CdR), and demethylation appeared after treatment with 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR for 72h. Real-time PCR showed that the expression levels of TFPI-2 gene mRNA were $1{\pm}0$, $1.49{\pm}0.14$, $1.86{\pm}0.09$ and $5.80{\pm}0.15$ (P<0.05) respectively. Western blotting analysis showed the relative expression levels of TFPI-2 protein were $0.12{\pm}0.01$, $0.23{\pm}0.02$, $0.31{\pm}0.02$, $0.62{\pm}0.03$ (P<0.05). TFPI-2 protein expression in A549 cells was gradually increased significantly with increase in the 5-Aza-CdR concentration. Conclusions: TFPI-2 gene promoter methylation results in the loss of TFPI-2 mRNA and protein expression in the non-small cell lung cancer cell line A549, and 5-Aza-CdR treatment could induce the demethylation of TFPI-2 gene promoter and restore TFPI-2 gene expression. These findings provide theoretic evidence for clinical treatment of advanced non-small cell lung cancer with the demethylation agent 5-Aza-CdR. TFPI-2 may be one molecular marker for effective treatment of advanced non-small cell lung cancer with 5-Aza-CdR.

Isolation and characterization of tick-borne Roseomonas haemaphysalidis sp. nov. and rodent-borne Roseomonas marmotae sp. nov.

Zhu Wentao,Zhou Juan,Lu Shan,Yang Jing,Lai Xin-He,Jin Dong,Pu Ji,Huang Yuyuan,Liu Liyun,Li Zhenjun,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2

Four novel Gram-negative, mesophilic, aerobic, motile, and cocci-shaped strains were isolated from tick samples (strains 546T and 573) and respiratory tracts of marmots (strains 1318T and 1311). The 16S rRNA gene sequencing revealed that strains 546T and 573 were 97.8% identical to Roseomonas wenyumeiae Z23T, whereas strains 1311 and 1318T were 98.3% identical to Roseomonas ludipueritiae DSM 14915T. In addition, a 98.0% identity was observed between strains 546T and 1318T. Phylogenetic and phylogenomic analyses revealed that strains 546T and 573 clustered with R. wenyumeiae Z23T, whereas strains 1311 and 1318T grouped with R. ludipueritiae DSM 14915T. The average nucleotide identity between our isolates and members of the genus Roseomonas was below 95%. The genomic G+C content of strains 546T and 1318T was 70.9% and 69.3%, respectively. Diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE) were the major polar lipids, with Q-10 as the predominant respiratory quinone. According to all genotypic, phenotypic, phylogenetic, and phylogenomic analyses, the four strains represent two novel species of the genus Roseomonas, for which the names Roseomonas haemaphysalidis sp. nov. and Roseomonas marmotae sp. nov. are proposed, with 546T (= GDMCC 1.1780T = JCM 34187T) and 1318T (= GDMCC 1.1781T = JCM 34188T) as type strains, respectively.

Study on a Novel Thermal Error Compensation System for High-Precision Ball Screw Feed Drive (1st Report: Model, Calculation and Simulation)

Zhe-Zhu Xu,최창,Long-jun Liang,Dong-Yang Li,류성기 한국정밀공학회 2015 International Journal of Precision Engineering and Vol.16 No.9

In general, in order to compensate the thermal error or the positioning error of a ball screw feed drive system, the actual temperature or positioning data feedback was needed. The traditional thermal error compensation system of ball screw feed drive is highly dependent on the feedback temperature or positioning data. Because of the overdependence to measuring technique, increasing of compensation system cost and decreasing of productivity level will be an inevitable trend in a machine tool. This paper presents a new approach in ball screw thermal error compensation system which can work without any temperature or positioning feedback. As the parts of the thermal error compensation system, component heat generation, compensation method, thermal model, mathematic model and calculation method were studied respectively. In order to verify correctness and generality of the developed thermal model and the thermal error compensation system, a series of simulations was carried out in several kinds of working condition. Through the series of simulations with the thermal model, calculation method and simulation conditions, deformation characteristics and thermal behavior of the prototype ball screw system have been obtained.

Study on a Novel Thermal Error Compensation System for High-Precision Ball Screw Feed Drive (2nd Report: Experimental Verification)

Zhe-Zhu Xu,Chang Choi,Long-jun Liang,Dong-Yang Li,류성기 한국정밀공학회 2015 International Journal of Precision Engineering and Vol.16 No.10

Real-time thermal error compensation of machine tool feed drive in general can be separated into three steps such as modeling, measurement and compensation. In the previous report, as the parts of the thermal error compensation system, component heat generation, compensation method, thermal model, mathematic model and calculation method were studied respectively. And a series of simulations was carried out in several kinds of working condition. And then, in order to discuss the correctness of the developed ball screw thermal error compensation system, a series of tests contains axial deformation, positioning accuracy, temperature variation and temperature distribution was carried out in the same working condition of prediction. As the results, the test data well confirmed the correctness of the developed ball screw thermal error compensation system.

Learning Curve and Complications of Unilateral Biportal Endoscopy: Cumulative Sum and Risk-Adjusted Cumulative Sum Analysis

Jinchao Xu,Dong Wang,Jidan Liu,Chengyue Zhu,Jianhang Bao,Wenshuo Gao,Wei Zhang,Hao Pan 대한척추신경외과학회 2022 Neurospine Vol.19 No.3

Objective: The purpose of this study was to investigate the learning curve and complications of unilateral biportal endoscopy (UBE) in the treatment of lumbar disc herniation (LDH) and lumbar spinal stenosis (LSS). Methods: This was a retrospective cohort analysis of 197 consecutive patients who received UBE unilateral laminotomy bilateral decompression (UBE-ULBD) or lumbar discectomy (UBE-LD) surgery, including 107 males and 90 females with an average age of 64.83 ± 14.29 years. Cumulative sum (CUSUM) and risk-adjusted cumulative sum analysis (RA-CUSUM) were used to evaluate the learning curve, with the occurrence of complications defined as surgical failure, and variables of different phase of the learning curve were compared. Results: The cutoff point of learning curve of UBE surgery was 54 cases according to CUSUM analysis. The learning curve of UBE-ULBD and UBE-LD were divided into 3 phases. The first cutoff points were 31 and 12 cases, and the second cutoff point were 67 and 32 cases respectively. With the progress of the learning curve, the operation time and postoperative hospital stays decreased. The visual analogue scale and Oswestry Disability Index at the last follow-up were significantly lower than that before surgery. The incidence of surgical failure was 6.11% and began to decrease after the 89th case based on RA-CUSUM analysis. The surgical failure rate decreased from 10.11% to 2.78 after the 89th case with significant different. Conclusion: UBE surgery is effective in the treatment of LDH and LSS with low incidence of complications. But a learning curve of at least 54 cases still required for mastering UBE surgery.