http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Xin, Zhi-Hong,Tian, Li,Zhu, Tian-Jiao,Wang, Wen-Liang,Du, Lin,Fang, Yu-Chun,Gu, Qian-Qun,Zhu, Wei-Ming 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.7
Two isocoumarin derivatives, stoloniferol A (1) and B (2), a known $5{\alpha}$, $8{\alpha}-epidioxy-23-methyl-(22E, 24R)-ergosta-6$, $22-dien-3{\beta}-ol$ (3), and a known dihydrocitrinone (4) were isolated from the ethyl acetate extract of the sea squirt-derived fungus, Penicillium stoloniferum QY2-10, and a halophilic fungus, Penicillium notatum B-52, respectively. Their structures were elucidated by spectroscopic methods and optical rotation. The stereochemistry of 2 was determined on the basis of different NOE experiments and chemical transformation. Compound 3 showed cytotoxicity against P388 cells, with an $IC_{50}$ value of 4.07 ${\mu}M$.
( Chun Fang ),( Tong Cao ),( Ying Shan ),( Ye Xia ),( Yong Ping Xin ),( Chang Yong Cheng ),( Houhui Song ),( John Bowman ),( Xiao Liang Li ),( Xiang Yang Zhou ),( Wei Huan Fang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
Listeria monocytogenes is a foodborne pathogen of considerable genetic diversity with varying pathogenicity. Initially, we found that the strain M7 was far less pathogenic than the strain Lm850658 though both are serovar 4a strains belonging to the lineage III. Comparative genomic approaches were then attempted to decipher the genetic basis that might govern the strain-dependent pathotypes. There are 2,761 coding sequences of 100% nucleotide identity between the two strains, accounting for 95.7% of the total genes in Lm850658 and 92.7% in M7. Lm850658 contains 33 specific genes, including a novel 20K prophage whereas strain M7 has 130 specific genes, including two large prophages (38K and 44K). To examine the roles of these specific prophages in pathogenicity, the 20K and 38K prophages were deleted from their respective strains. There were virtually no differences of pathogenicity between the deletion mutants and their parent strains, although some putative virulent factors like VirB4 are present in the 20K region or holin-lysin in the 38K region. In silico PCR analysis of 29 listeria genomes show that only strain SLCC2540 has the same 18 bp integration hotspot as Lm850658, whereas the sequence identity of their 20K prophages is very low (21.3%). The 38K and 44K prophages are located in two other different hotspots and are conserved in low virulent strains M7, HCC23, and L99. In conclusion, the 20K and 38K prophages of L. monocytogenes serovar 4a strains Lm850658 and M7 are not related to virulence but contribute to genetic diversity.
Characterization of a Late Gene, ORF60 from Bombyx mori Nucleopolyhedrovirus
Du, Meng-Fang,Yin, Xin-Ming,Guo, Zhong-Jian,Zhu, Liang-Jun Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6
Open reading frame 60 of Bombyx mori nucleopolyhedrovirus (Bm60) is located between 56,673 and 57,479 bp in the BmNPV genome which encodes 268 amino acid residues with predicted molecular weight of 31.0 kDa. Bm60 and its homologues have been identified in 11 completely sequenced lepidopteran NPVs. The transcript of Bm60 was detected by RT-PCR at 18-72 h post-infection (p.i.), while the corresponding protein could be detected at 24-72 h p.i. in BmNPV-infected BmN cells by Western blot analysis using a polyclonal antibody against Bm60. The expression of Bm60 was inhibited in the presence of Ara-c, an inhibitor of viral DNA synthesis. These results together indicated that Bm60 was a late gene. The size of Bm60 product was found to be a 31 kDa in BmNPV-infected BmN cells, consistent with predicted molecular weight. Immuno-fluoresence analysis showed that the Bm60 product was first detected in the cytoplasm at 24 h p.i and also located in nucleus during later infection. In conclusion, the available data suggest that Bm60 is a functional ORF of BmNPV and encodes a 31kDa protein expressed in the later stage of infection cycle.
Hao Wang,Liang Wang,Xin Qian,Ke Cao,Ying Xu,Yi Fang,Liyun Cui 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.9
Grinding brick waste into powder for use as a supplementary cementitious material has shown to be a promising recycling method for the reuse of construction and demolition waste.However, the strength and durability of concrete containing recycled brick powder (RBP) will significantly decrease due to the poor pozzolanic activity of RBP, limiting its wider applications. This study attempted to overcome these defects through metakaolin (MK) addition. The hydration, compressive strength, durability, and environmental impact of eco-friendly mortars containing RBP and MK were investigated. The results showed that MK promoted the early-stage hydration of cement, creating a suitable hydration environment for the hydration of RBP at a later age. As a result, the volume fractions of low-density calcium silicate hydrate (C-S-H) and high-density C-S-H increased according to the nanoindentation test results. In addition, the strength, chloride penetration resistance, and reinforcement bar corrosion resistance of the blended mortars obviously improved due to the synergistic effect of RBP and MK. Lastly, we achieved CO2 emissions that were 22.1 – 30.2% lower and energy consumption that was 21.4 – 27.0% lower by incorporating 20% RBP and 5 – 15% MK.
Zhi-Hong Xin,Li-Tian,Tian-jiao Zhu,Wen-Liang Wang,Lin Du,Yu-chun Fang,Qian-Qun Gu,Wei-Ming Zhu 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.7
Two isocoumarin derivatives, stoloniferol A (1) and B (2), a known 5α, 8α-epidioxy-23-methyl- (22E, 24R)-ergosta-6, 22-dien-3β-ol (3), and a known dihydrocitrinone (4) were isolated from the ethyl acetate extract of the sea squirt-derived fungus, Penicillium stoloniferum QY2-10, and a halophilic fungus, Penicillium notatum B-52, respectively. Their structures were elucidated by spectroscopic methods and optical rotation. The stereochemistry of 2 was determined on the basis of different NOE experiments and chemical transformation. Compound 3 showed cytotoxicity against P388 cells, with an IC50 value of 4.07 µM.
Jiang, Chang,Liao, Fang-Xin,Rong, Yu-Ming,Yang, Qiong,Yin, Chen-Xi,He, Wen-Zhuo,Cai, Xiu-Yu,Guo, Gui-Fang,Qiu, Hui-Juan,Chen, Xu-Xian,Zhang, Bei,Xia, Liang-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13
Objective: To compare the efficacy of taxane-based regimens in the first line setting retrospectively in Chinese patients with recurrent and/or metastatic esophageal cancer. Methods: We analyzed 102 recurrent and/or metastatic esophageal cancer patients who received taxanes-based regimens in a first-line setting from January 2009 to December 2013. Sixteen (15.7%) patients were administered Nab-PTX based chemotherapy and 86 patients (84.3%) received paclitaxel (PTX) or docetaxel (DTX) based chemotherapy. Patients in the PTX/DTX group could be further divided into TP (71 patients) and TPF (15 patients) groups. Results: The objective response rate (ORR) of all patients was 20.6%, and the disease control rate (DCR) was 67.6%. The median overall survival (OS) was 10.5 months (95% CI 10.1-16.4) and the median progression-free survival (PFS) was 6.04 months (95% CI 5.09-7.91). The DCR was higher in the TPF group than the TP group (93.3% vs. 59.1%; p = 0.015 ). There were no significant differences in ORR, OS, and PFS among Nab-PTX, TPF and TP groups. Conclusions: The three regimens of Nab-PTX based, TP and TPF proved active in a first line setting of Chinese patients with recurrent and/or metastatic esophageal cancer, and should thus be regarded as alternative treatments.