Stem-leaf saponins from Panax notoginseng counteract aberrant autophagy and apoptosis in hippocampal neurons of mice with cognitive impairment induced by sleep deprivation

Cao, Yin,Yang, Yingbo,Wu, Hui,Lu, Yi,Wu, Shuang,Liu, Lulu,Wang, Changhong,Huang, Fei,Shi, Hailian,Zhang, Beibei,Wu, Xiaojun,Wang, Zhengtao The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.3

Backgroud: Sleep deprivation (SD) impairs learning and memory by inhibiting hippocampal functioning at molecular and cellular levels. Abnormal autophagy and apoptosis are closely associated with neurodegeneration in the central nervous system. This study is aimed to explore the alleviative effect and the underlying molecular mechanism of stem-leaf saponins of Panax notoginseng (SLSP) on the abnormal neuronal autophagy and apoptosis in hippocampus of mice with impaired learning and memory induced by SD. Methods: Mouse spatial learning and memory were assessed by Morris water maze test. Neuronal morphological changes were observed by Nissl staining. Autophagosome formation was examined by transmission electron microscopy, immunofluorescent staining, acridine orange staining, and transient transfection of the tf-LC3 plasmid. Apoptotic event was analyzed by flow cytometry after PI/annexin V staining. The expression or activation of autophagy and apoptosis-related proteins were detected by Western blotting assay. Results: SLSP was shown to improve the spatial learning and memory of mice after SD for 48 h, accomanied with restrained excessive autophage and apoptosis, whereas enhanced activation of phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway in hippocampal neurons. Meanwhile, it improved the aberrant autophagy and apoptosis induced by rapamycin and re-activated phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling transduction in HT-22 cells, a hippocampal neuronal cell line. Conclusion: SLSP could alleviate cognitive impairment induced by SD, which was achieved probably through suppressing the abnormal autophagy and apoptosis of hippocampal neurons. The findings may contribute to the clinical application of SLSP in the prevention or therapy of neurological disorders associated with SD.

Development and manufacturing of a surgical simulator based on virtual reality

Di Cao,Ao Zhang,Xiaojun Chen 한국생산제조학회 2014 한국생산제조시스템학회 학술발표대회 논문집 Vol.2014 No.9(2)

Stem-leaf saponins from Panax notoginseng counteract aberrant autophagy and apoptosis in hippocampal neurons of mice with cognitive impairment induced by sleep deprivation

Yin Cao,Yingbo Yang,Hui Wu,Yi Lu,Shuang Wu,Lulu Liu,Changhong Wang,Fei Huang,Hailian Shi,Beibei Zhang,Xiaojun Wu,Zhengtao Wang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.3

Backgroud: Sleep deprivation (SD) impairs learning and memory by inhibiting hippocampal functioningat molecular and cellular levels. Abnormal autophagy and apoptosis are closely associated with neurodegenerationin the central nervous system. This study is aimed to explore the alleviative effect and theunderlying molecular mechanism of stemeleaf saponins of Panax notoginseng (SLSP) on the abnormalneuronal autophagy and apoptosis in hippocampus of mice with impaired learning and memory inducedby SD. Methods: Mouse spatial learning and memory were assessed by Morris water maze test. Neuronalmorphological changes were observed by Nissl staining. Autophagosome formation was examined bytransmission electron microscopy, immunofluorescent staining, acridine orange staining, and transienttransfection of the tf-LC3 plasmid. Apoptotic event was analyzed by flow cytometry after PI/annexin Vstaining. The expression or activation of autophagy and apoptosis-related proteins were detected byWestern blotting assay. Results: SLSP was shown to improve the spatial learning and memory of mice after SD for 48 h,accomanied with restrained excessive autophage and apoptosis, whereas enhanced activation of phosphoinositide3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway in hippocampalneurons. Meanwhile, it improved the aberrant autophagy and apoptosis induced by rapamycinand re-activated phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling transductionin HT-22 cells, a hippocampal neuronal cell line. Conclusion: SLSP could alleviate cognitive impairment induced by SD, which was achieved probablythrough suppressing the abnormal autophagy and apoptosis of hippocampal neurons. The findings maycontribute to the clinical application of SLSP in the prevention or therapy of neurological disordersassociated with SD.

Distance Estimation Methods in Vehicular Application: An Experimental Study

Yiwen Cao,Xinglong Lu,Zhonghua Zhao,Xiaojun Ji,Yilin Yan 제어로봇시스템학회 2018 제어로봇시스템학회 국제학술대회 논문집 Vol.2018 No.10

Passive Entry and Passive Start (PEPS) system allows drivers to enter the vehicle and start the engine without activating key fob. With growing demand for location-based service and rapid development of Bluetooth Low Energy (BLE), BLE PEPS with localization service becomes a promising choice for next generation of PEPS. This paper studies the critical problem of localizing the vehicle for user based on BLE received signal strength (RSS) provided by preinstalled BLE Access Points (APs) inside vehicle. Multiple APs are employed to reduce the error caused by RSS fluctuation on distance estimation. Fusion algorithms including Extended Kalman Filter, Monte Carlo Localization and Iterative Trilateration are compared based on experiments in vehicle. Experimental results show that distance estimation based on Extended Kalman Filter gets high accuracy in both long distance and short distance scenario.

Axial Compressive Performance of CFRP Confined Self-stressing High-strength Concrete Cylinders

Qi Cao,Xiaojun Liu,Rongxiong Gao 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.9

Due to the advantages of FRP (fiber reinforced polymer), such as light weight, high strength and corrosion resistance, FRP-confined concrete columns are gradually applied in civil engineering structures and have drawn wide attention from the engineering community. In order to avoid the stress hysteresis of CFRP (carbon fiber reinforced polymer) and make full use of FRP materials, expansive agent was added to concrete to make self-stressing high-strength concrete. In this study, the axial compression performance of 18 CFRP-confined self-stressing high-strength concrete cylinders was examined. The parameters include the CFRP layers and the level of prestress (with or without prestress). Experimental results show that 3.53 − 5.34 MPa prestress in concrete and 799.3 − 1,584.2 MPa prestress in CFRP are produced in the composite cylinder. The stress-strain curves obtained from the experiment shows that the intercept stress, inflection stress and peak stress of the self-stressing specimens are all higher than those of the non-prestressed specimens. With the application of prestress, the utilization ratio of CFRP increases as well. In the theoretical calculation of intercept and peak stress, by modifying the existing models, the experimental results are in good agreement with the calculated results.

Macro-/micro-environment-sensitive chemosensing and biological imaging

Yang, Zhigang,Cao, Jianfang,He, Yanxia,Yang, Jung Ho,Kim, Taeyoung,Peng, Xiaojun,Kim, Jong Seung The Royal Society of Chemistry 2014 Chemical Society reviews Vol.43 No.13

<P>Environment-related parameters, including viscosity, polarity, temperature, hypoxia, and pH, play pivotal roles in controlling the physical or chemical behaviors of local molecules. In particular, in a biological environment, such factors predominantly determine the biological properties of the local environment or reflect corresponding status alterations. Abnormal changes in these factors would cause cellular malfunction or become a hallmark of the occurrence of severe diseases. Therefore, in recent years, they have increasingly attracted research interest from the fields of chemistry and biological chemistry. With the emergence of fluorescence sensing and imaging technology, several fluorescent chemosensors have been designed to respond to such parameters and to further map their distributions and variations <I>in vitro</I>/<I>in vivo</I>. In this work, we have reviewed a number of various environment-responsive chemosensors related to fluorescent recognition of viscosity, polarity, temperature, hypoxia, and pH that have been reported thus far.</P> <P>Graphic Abstract</P><P>We have summarized the research progress on fluorescent sensors responsive to environmental factors, including local viscosity, polarity, temperature, hypoxia and pH. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cs00051j'> </P>

Concentrated Multi-nozzle Electrospinning

Yuekun Zheng,Huatang Cao,Zhou Zhou,Xuecui Mei,Lingke Yu,Xiaojun Chen,Gonghan He,Yang Zhao,Dezhi Wu,Daoheng Sun 한국섬유공학회 2019 Fibers and polymers Vol.20 No.6

The multi-nozzle electrospinning is under extensive investigations because it is an easy way to enhance theproductivity and also feasible to produce special structure fibers such as core-shell fibers and to fabricate composite fibers ofthose polymers that cannot form blend solution in common solvent. Control over the multi-nozzle electrospinning fibersdeposition has attracted increasing attentions. The most common method was to use the auxiliary electrode. However, theconcentrated effect of the works of control multi-nozzle electrospinning deposit was inconspicuous. To enhance thecontrolling of multi-nozzle electrospinning deposition, a set-up based oppositely charged electrospinning was designed. Inthis set-up the air flow was used to transport neutralized nanofibers. This electrospinning method was named oppositelycharged and air auxiliary electrospinning (OCAAES). The capacity of OCAAES in deposition area and pattern controllingwere investigated. By the OCAAES, concentrated and several patterned nanofibers deposition were fabricated. Resultsshowed that nanofiber deposition area and pattern of multi-nozzle electrospinning could be controlled actively, and nanofiberdeposition could be fabricated in a quick thickening rate.

Ionic Liquid Induced Supramolecular Self-Assembly of Poly(3,4-ethylenedioxythiophene)/ Poly(4-styrenesulfonate) Thin Films with Enhanced Conductivity and Tunable Nanoporosity

Liping Zhao,Jishan Qiu,Xiaojun Cao,Wenyong Dong,Jichun You,Yongjin LI 한국고분자학회 2013 Macromolecular Research Vol.21 No.4

We present a facile strategy, for the first time as the best of our known, to prepare high conducting poly(3,4-ethylenedioxythiophene)/poly(4-styrenesulfonate) (PEDOT:PSS) film with a novel nanoporous morphology directly from a commercial PEDOT:PSS dispersion. Both conducting properties and surface morphology of PEDOT:PSS film can be systematically tunable by simply controlling addition of an ionic liquid, 1,3-dihydroxy-2-methylimidazolium bis(trifluoromethylsulfonyl)-imide (DHIL). The electrical conductivity increases from 0.07 for pristine PEDOT:PSS to 55 S cm-1 after addition of 2 wt% DHIL, with no necessary of any further heat treatment, which is around 800 times increase in the electrical conductivity. On the other hand, the discrete compact PEDOT:PSS film is gradually transformed into the nanoporous film with addition of DHIL. It is considered that such size-tunable porous PEDOT:PSS films with high surface area as well as high conductivity combining solution-processibility show great potential in applications that require high interfacial area, such as flexible electronic components, nextgeneration catalytic, and separation supports.

Novel splice isoforms of pig myoneurin and their diverse mRNA expression patterns

Guo, Xiaohong,Li, Meng,Gao, Pengfei,Cao, Guoqing,Cheng, Zhimin,Zhang, Wanfeng,Liu, Jianfeng,Liu, Xiaojun,Li, Bugao Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10

Objective: The aim of this study was to clone alternative splicing isoforms of pig myoneurin (MYNN), predict the structure and function of coding protein, and study temporal and spatial expression characteristics of each transcript. Methods: Alternative splice isoforms of MYNN were identified using RNA sequencing (RNA-seq) and cloning techniques. Quantitative real-time polymerase chain reaction (qPCR) was employed to detect expression patterns in 11 tissues of Large White (LW) and Mashen (MS) pigs, and to study developmental expression patterns in cerebellum (CE), stomach (ST), and longissimus dorsi (LD). Results: The results showed that MYNN had two alternatively spliced isoforms, MYNN-1 (GenBank accession number: KY470829) and MYNN-2 (GenBank accession number: KY670835). MYNN-1 coding sequence (CDS) is composed of 1,830 bp encoding 609 AA, whereas MYNN-2 CDS is composed of 1,746 bp encoding 581 AA. MYNN-2 was 84 bp less than MYNN-1 and lacked the sixth exon. MYNN-2 was found to have one $C_2H_2$ type zinc finger protein domain less than MYNN-1. Two variants were ubiquitously expressed in all pig tissues, and there were significant differences in expression of different tissues (p<0.05; p<0.01). The expression of MYNN-1 was significantly higher than that of MYNN-2 in almost tissues (p<0.05; p<0.01), which testified that MYNN-1 is the main variant. The expression of two isoforms decreased gradually with increase of age in ST and CE of MS pig, whereas increased gradually in LW pig. In LD, the expression of two isoforms increased first and then decreased with increase of age in MS pig, and decreased gradually in LW pig. Conclusion: Two transcripts of pig MYNN were successfully cloned and MYNN-1 was main variant. MYNN was highly expressed in ST, CE, and LD, and their expression was regular. We speculated that MYNN plays important roles in digestion/absorption and skeletal muscle growth, whereas the specific mechanisms require further elucidation.

Comparative transcriptome analyses provide insights into the adaptation mechanisms to acute salt stresses in juvenile Sinonovacula constricta

Bin Ma,Zhaoshou Ran,Xiaorong Xu,Jilin Xu,Kai Liao,Jiayi Cao,Xiaojun Yan 한국유전학회 2019 Genes & Genomics Vol.41 No.5

Background Sinonovacula constricta is an economically important bivalve species in China, Korea and Japan that widely resides in estuarine and coastal areas where salinity fluctuates rapidly. However, little is known about its adaptation mechanisms to acute salt stresses. Objective To reveal the underlying molecular mechanisms involved in acute salt stresses in juvenile S. constricta. Methods Nine cDNA libraries (triplicate each trial) were established from juvenile S. constricta, which were subjected to low salinity (5 psu), optimal salinity (15 psu) and high salinity (25 psu) for 6 h, respectively. Results Illumina sequencing generated 478,587,310 clean reads totally, which were assembled into 427,057 transcripts of 246,672 unigenes. Compared with the control, 1259 and 2163 differentially expressed genes (DEGs) were identified under acute low and high salt stresses, respectively. GO and KEGG enrichment analyses of DEGs revealed that several key metabolic modulations were mainly responsible for the acute salt stresses. According to the significantly highlighted KEGG pathways, some key DEGs were identified and discussed in details. Notably, based on which, some potential osmolytes were further speculated. Conclusion Here, we carried out a unique report of comparative transcriptome analyses in juvenile S. constricta in response to acute salt stresses. The identified DEGs and their significantly enriched GO terms and KEGG pathways were critical for understanding and further investigating the underlying the physical and biochemical performances, and ultimately facilitated S. constricta breeding. Besides, the transcriptome data greatly enriched the genetic information of S. constricta, which were valuable for promoting its molecular biology researches.