WRKY Transcription Factor Functions as a Transcriptional Regulator of Xylanase Inhibitor RIXI, Involved in Rice Disease Resistance to Magnaporthe oryzae

Xiao‑qi Gao,Xiang‑yu Sun,Yao‑yao Peng,Ying‑ying Huang,Ming‑Qi Liu,Xiao‑yan Weng 한국식물학회 2020 Journal of Plant Biology Vol.63 No.3

Xylanase inhibitors are known to play important roles in response to fungal pathogens attack. WRKY transcription factors are also involved in plant defence against pathogens. However, whether WRKY transcription factors interacting with rice xylanase inhibitor RIXI remains unknown. 13 W-boxes, binding sites for WRKY transcription factors were found in RIXI promoter sequence of rice by analyzing RIXI promoter. ChIP-PCR analysis and dual luciferase reporter system assay were used to identify interaction between xylanase inhibitor RIXI and WRKY transcription factors. Results showed OsWRKY6 and OsWRKY46 directly bound to RIXI promoter and regulated RIXI expression diferently. Over-expressing OsWRKY6 and OsWRKY46 transgenic plants were achieved, and OsWRKY6 and OsWRKY46 overexpressed transgenic lines showed higher expression levels of RIXI and higher resistance to Magnaporthe oryzae G11 (M. oryzae G11) compared to wild type (WT). Increased RIXI expression in OsWRKY6 and OsWRKY46 overexpressed transgenic lines was accompanied by up-regulation of pathogenesis-related genes (PR genes). Moreover, higher levels of H2O2 and higher activities of antioxidant enzymes were found in transgenic lines compared to WT plants. Results indicated that over-expression of OsWRKY6 and OsWRKY46 in transgenic lines maybe enhanced rice disease resistance by regulating RIXI expression.

Study on Energy Regeneration of Hybrid Hydraulic Excavator using Hydraulic Transformer

Ying-Xiao Yu,Ahn Kyoung Kwan 제어로봇시스템학회 2016 제어로봇시스템학회 국제학술대회 논문집 Vol.2016 No.10

Facing the energy crisis and environmental problems, saving energy of construction machinery, especially in the hydraulic excavator is very important. Regeneration of the potential energy is an effective solution to save energy. However, the conventional hybrid excavator can’t regenerate the potential energy. This paper proposes a new structure of hydraulic excavator as well as a control strategy. The focus of the paper is using hydraulic transformer to regenerate energy. The simulation is carried out by the AMESim software. From the simulation results, this new system has a high efficiency for energy saving.

In situ chemical imaging of environmental, energy, and biological liquid surfaces and interfaces

Xiao-Ying YU 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Chinese patent herbal medicine (Shufeng Jiedu capsule) for acute upper respiratory tract infections: A systematic review and meta-analysis

Ying-ying Zhang,Ru-yu Xia,Shi-bing Liang,Xiao-yang Hu,Meng-yuan Dai,Yi-lin Li,Le-yi Zhao,Michael Moore,Yu-tong Fei,Jian-ping Liu 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.3

Background: Shufeng Jiedu capsule has been widely used in China for acute upper respiratory tract infections (AURTIs). The aim of this study was to evaluate its effectiveness and safety for AURTIs. Methods: Randomized controlled trials comparing SFJD with conventional drug for patients with AURTIs were included. Eight databases were searched from their inceptions to February 2021. Data was synthesized using risk ration (RR) or mean difference (MD) with their 95% confidence interval (CI). The primary outcome was resolution time of typical symptoms. Results: Twenty-five RCTs involving 3410 patients were included. SFJD in combination with conventional drug was associated with; in common cold shortening the duration of fever (MD −1.54 days, 95% CI [−2.15,−0.92], I2 = 80%, n = 385, 3 trials) and cough (MD −1.22 days, 95% CI [−1.52, −0.93]); in herpangina, shortening the duration of fever (MD -0.68 days, 95% CI [−1.15, −0.21], I2 = 68%, n = 140, 2 trials) and blistering (MD −0.99 days, 95% CI [−1.23, −0.76], n = 386, 3 trials); in acute tonsillitis and acute pharyngitis shortening the duration of fever (MD −1.13 days, 95% CI [−1.36, −0.90], I2 = 33%, n = 688, 7 trials) and sore throat (MD −1.13 days, 95% CI [−1.40, −0.86], I2 = 84.1%, n = 1194, 10 trials). SFJD also improving their cure rate with a range (1–5 days). No serious adverse events were reported. Conclusion: Low certainty evidence suggests that SFJD appears to shorten the duration of symptoms in AURTIs, improve cure rate and seems safe for application. However, high quality placebo controlled trials are warranted to confirm its benefit.

Optimization of energy regeneration of hybrid hydraulic excavator boom system

Yu, Ying-Xiao,Ahn, Kyoung Kwan Elsevier 2019 Energy Conversion and Management Vol.183 No.-

<P><B>Abstract</B></P> <P>Saving energy is important and necessary for hydraulic excavators. This paper proposed a novel energy regeneration boom system. A variable displacement hydraulic motor and flow control valve were used to regulate the torque and speed of the generator, and to regulate flow through the hydraulic motor. A control strategy was proposed to calculate the optimal displacement of the hydraulic motor. The hydro-mechanical efficiency map and volumetric efficiency map were used to estimate the speed and torque of the generator instead of using speed and torque sensor. The resulting energy regeneration efficiency ranged from 33.8% to 57.4%, which cannot be realized in conventional boom system. Compared with conventional energy regeneration boom system, the improvement of energy regeneration efficiency with the proposed system was 3.2% to 4.1% for low and moderate velocities. A control strategy has been proposed to decrease the setup power of generator and ensure system safety in large load and high velocity mode.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel energy regeneration boom system is proposed in this paper. </LI> <LI> A control strategy is developed to optimize the energy regeneration efficiency. </LI> <LI> Efficiency is higher than the conventional energy regeneration system. </LI> <LI> The setup power of generator is decreased. </LI> <LI> System safety is improved in large load and high velocity mode. </LI> </UL> </P>

A Study on the Energy Regeneration System of Boom for Hybrid Hydraulic Excavator

Ying-Xiao Yu,Debdatta Das,Truong B. N. M,Ahn Kyoung Kwan 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10

Nowadays, energy crisis and pollution have become a serious problem in the world. Saving energy of construction machinery, especially the hydraulic excavator is very essential. Potential gravitational energy regeneration is an effective solution to reduce the energy consumption of the hydraulic excavator. This paper proposes a new hybrid hydraulic excavator boom system and designs a new control strategy. In this system have three modes for boom down. The proposed energy regeneration system is verified through simulation result which is done in the AMESim software. By analyzing simulation result, the proposed system can perform very efficiently. Meanwhile, the proposed system is also compared with conventional one. By comparing and analyzing the simulation results, it seen that the new system has a better efficiency and decrease the energy consumption significantly. All parameters are taken from the real system.

Ginsenoside Rg3 in combination with artesunate overcomes sorafenib resistance in hepatoma cell and mouse models

Ying-Jie Chen,Jia-Ying Wu,Yu-Yi Deng,Ying Wu,Xiao-Qi Wang,Amy Sze-man Li,Lut Yi Wong,Xiuqiong Fu,Zhi-Ling Yu,Chun Liang 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.3

Sorafenib is effective in treating hepatoma, but most patients develop resistance to it. STAT3signaling has been implicated in sorafenib resistance. Artesunate (ART) and 20(R)-ginsenoside Rg3 (Rg3)have anti-hepatoma effects and can inhibit STAT3 signaling in cancer cells. This study aimed to evaluatethe effects of Rg3 in combination with ART (Rg3-plus-ART) in overcoming sorafenib resistance, and toexamine the involvement of STAT3 signaling in these effects. Methods: Sorafenib-resistant HepG2 cells (HepG2-SR) were used to evaluate the in vitro anti-hepatomaeffects of Rg3-plus-ART. A HepG2-SR hepatoma-bearing BALB/c-nu/nu mouse model was used to assessthe in vivo anti-hepatoma effects of Rg3-plus-ART. CCK-8 assays and Annexin V-FITC/PI double stainingwere used to examine cell proliferation and apoptosis, respectively. Immunoblotting was employed toexamine protein levels. ROS generation was examined by measuring DCF-DA fluorescence. Results: Rg3-plus-ART synergistically reduced viability of, and evoked apoptosis in HepG2-SR cells, andsuppressed HepG2-SR tumor growth in mice. Mechanistic studies revealed that Rg3-plus-ART inhibitedactivation/phosphorylation of Src and STAT3 in HepG2-SR cultures and tumors. The combination alsodecreased the STAT3 nuclear level and induced ROS production in HepG2-SR cultures. Furthermore, overactivation of STAT3 or removal of ROS diminished the anti-proliferative effects of Rg3-plus-ART, andremoval of ROS diminished Rg3-plus-ART's inhibitory effects on STAT3 activation in HepG2-SR cells. Conclusions: Rg3-plus-ART overcomes sorafenib resistance in experimental models, and inhibition of Src/STAT3 signaling and modulation of ROS/STAT3 signaling contribute to the underlying mechanisms. Thisstudy provides a pharmacological basis for developing Rg3-plus-ART into a novel modality for treatingsorafenib-resistant hepatoma.

Protectin DX increases alveolar fluid clearance in rats with lipopolysaccharideinduced acute lung injury

Xiao-Jun Zhuo,Yu Hao,Fei Cao,Song-Fan Yan,Hui Li,Qian Wang,Bi-Huan Cheng,Bin-Yu Ying,Bin-Yu Ying,Sheng-Wei Jin 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Acute respiratory distress syndrome is a life-threatening critical syndrome resulting largely from the accumulation of and the inability to clear pulmonary edema. Protectin DX, an endogenously produced lipid mediator, is believed to exert anti-inflammatory and pro-resolution effects. Protectin DX (5 μg/kg) was injected i.v. 8 h after LPS (14 mg/kg) administration, and alveolar fluid clearance was measured in live rats (n = 8). In primary rat ATII epithelial cells, protectin DX (3.605 × 10−3 mg/l) was added to the culture medium with LPS for 6 h. Protectin DX improved alveolar fluid clearance (9.65 ± 1.60 vs. 15.85 ± 1.49, p < 0.0001) and decreased pulmonary edema and lung injury in LPSinduced lung injury in rats. Protectin DX markedly regulated alveolar fluid clearance by upregulating sodium channel and Na, K-ATPase protein expression levels in vivo and in vitro. Protectin DX also increased the activity of Na, K-ATPase and upregulated P-Akt via inhibiting Nedd4–2 in vivo. In addition, protectin DX enhanced the subcellular distribution of sodium channels and Na, K-ATPase, which were specifically localized to the apical and basal membranes of primary rat ATII cells. Furthermore, BOC-2, Rp-cAMP, and LY294002 blocked the increased alveolar fluid clearance in response to protectin DX. Protectin DX stimulates alveolar fluid clearance through a mechanism partly dependent on alveolar epithelial sodium channel and Na, K-ATPase activation via the ALX/PI3K/Nedd4–2 signaling pathway.

Establishment of a model to evaluate the nutritional quality of Bombyx mori Linnaeus (Lepidoptera, Bombycidae) pupae lipid based on principal components

Yu-Xiao Zou,Teng-Gen Hu,Ying Shi,Jun Liu,Li-Xia Mu,Yang Xiao,Sen-Tai Liao 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

Silkworm pupae are a source of edible lipid with a high content of unsaturated fatty acids (UFAs) (70% of total lipid content) and are becoming a focus of pharmaceutical and dietary research in the functional oil field. To study the nutritional value of different silkworm strains, principal component analysis (PCA) was used to establish a mathematical model and cluster analysis on silkworm pupae lipid (SPL) in 90 strains based on Z-score. Single-factor correlation analysis indicated that the PUFAs content was significantly positively correlated with the α-linolenic acid (ALA) (ρ=0.98) and negatively correlated with the oleic acid (OA) (ρ=−0.73) contents, and comprehensive of principal components mathematical model (PC =0.148ZX1 +0.00 2ZX2 −0.197ZX3 −0.338ZX4 +0.220ZX5 +0.304ZX6 +0.314ZX7, ZXi is the standard score (Z-score) of X1, which was calculated as follow: ZXi = (Xi −−X )/S, where X1: palmitic acid, X2: palmitoleic acid, X3: stearic acid, X4: OA, X5: linoleic acid, X6: ALA, X7: PUFAs.−X is the arithmetic mean content of variable i, and S is the standard deviation of variable i for all samples included in the analysis.) showed the amount of ALA and PUFAs were the key factors determining the nutritional value of the pupae lipid. Cluster analysis on the composite scores calculated by the model of the principal component divided the 90 strains into three groups. A cluster of 6 strains (XHR, XIFF, ZX, YT, YO and BTN) with the best nutritional value was characterized by high contents of ALA and PUFAs and a low content of oleic acid.

Energy Saving of an Electric Forklift with Hydraulic Accumulator

Ying-Xiao Yu,Kyoung Kwan Ahn 제어로봇시스템학회 2019 제어로봇시스템학회 국제학술대회 논문집 Vol.2019 No.10

Energy saving of construction machinery is necessary to reduce the energy consumption and pollution. A novel hydraulic hybrid forklift for energy saving is proposed in this paper, as well as the control strategy. A hydraulic accumulator is used in the system to store the potential energy. Then the energy could be reused in the next cycle of work. The maximum energy saving efficiency could reach 56% in one cycle. The total energy saving efficiency is 19%. The system could save much energy with low cost. So, it is easy to be used in the real forklift system.