Chinese Pediatric Reference Intervals for Serum Cortisol on IMMULITE 2000

Ling-Li Wang,Li-Yue Zhang,Hui-Yan Wang,Huai-Kai Wen,Hong-Qun Tao,Xiao-wei Zhao 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.1

Clinical interpretation of the test results for cortisol based on continuous reference intervals with appropriate partitions improves pediatric diagnosis; however, these values are available only for Caucasians. To develop the pediatric reference intervals for Chinese population, we examined the serum cortisol levels in 1,143 healthy Chinese children aged 4–18 years (566 boys and 577 girls), using an IMMULITE 2000 Immunoassay System (Siemens Healthcare GmbH). Phlebotomy was performed at 7–9 a.m. for 284 boys and 287 girls and at 1–3 p.m. for the others. They were divided into four age groups according to the Clinical and Laboratory Standards Institute guideline EP28-A3c, with the last group further stratified according to sampling time. Separate reference intervals of 49.6–323.7, 70.9–395.3, and 90.1–448.7 nmol/L were established for children aged 4–8, 9–12, and 13–15 years, respectively. Further, reference intervals of 118.2–464.7 and 71.4–446.7 nmol/L were established for morning and afternoon cortisol levels, respectively, in children aged 16–18 years. Further studies are necessary to transfer and validate these reference intervals in other analytical systems and pediatric populations, and to allow for broader applications.

연줄과 "꽌시(關係)"의 차이 : 한국과 중국의 사회연결망 비교

Xiao Ling Wang 慶熙大學校 社會科學硏究院 2007 社會科學硏究 Vol.33 No.2

이 글은 사회연결망 분석의 시각에서 한국의 '연줄'과 중국의 '꽌시'에 나타나는 특징에 대해서 비교연구를 실기했으며 다음과 같은 연구 결과를 발견했다: '연줄'과 '꽌시'는 모두 친족 비중이 매우 높은 폐쇄적인 연결망이다. 그러나 두 연결망 사이에 많은 차이점이 존재하다 산업화의 과정 중에서 한국인은 자신을 소외시키는 완전한 개인주의를 피하면서 혈연, 지연, 학연 등 소집단을 찾아 '집단화된 개인적 연줄'을 선택했다. 이에 비해서 중국인은 개인주의를 선택했다. 한국인의 '연줄'은 혈연, 학연처럼 귀속적인 특성을 가지고 있으나 중국인의 '꽌시'는 오랜 시간의 교제를 통해서 만들어진 친구의 비율과 중요성이 훨씬 높다. 통계분석을 한 결과 한국의 경우에 일상생활 중에서 흔히 말하는 '연줄'의 3가지 구성 요소 혈연, 지연과 학연 중의 지연의 영향력이 뚜렷하지 않다. 학연은 한국인의 연결망 크기와 연결망 구조에 가장 큰 영향을 미친다. 중국의 경우에 경제 개혁의 진전도가 중요한 요소로 작용한다. 발전된 도시일수록 '꽌시'연결망이 작아지며 사람들은 가족만 의지하게 된다. The article compares the 'yeongzul' in Korea and the 'guanxi' in China from the perspective of social networks. It concludes that although both are the closed social networks based on consanguinity, there are significant differences. First, during industrialization the Korean people choose the 'collectivized individualistic networks' which are based on consanguinity, regionalism, and graduation schools, while the Chinese choose the 'completely individualistic networks'. Second, the 'yeongzul' networks are generally predetermined by the sameness of consanguinity, regionalism, and graduation school, while the 'guanxi' networks are shaped through long-term acquaintance of people who have a relatively higher proportion within the networks. Third, in the daily life, education, instead of regionalism whose influence is not remarkable, plays a dominant role in forming the scale and structure of the 'yeongzul' networks, while in China, the deeper the economic reform, the smaller the 'guanxi' networks and the more based on family members.

Metabolic Engineering of Vitamin C Production in Arabidops

Ling Xiao,Ying Xiao,Zinan Wang,Hexin Tan,Kexuan Tang,Lei Zhang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

Vitamin C (L-ascorbic acid, AsA) is a compound which provides major nutritional value, both for plants and humans. In this study, three distinct metabolic engineering strategies, including overexpression of biosynthesis enzyme, suppression of catabolism enzyme and switching the sub-cellular localization of compartment enzyme, were employed to enhance the production of AsA in Arabidopsis thaliana. The results showed that (1) overexpression of L-Galactose-1-P Phosphatase (GalPPase) enhanced AsA content to 3.96 μmol/g FW, which was 1.6-fold more than that in their wild-type (WT) counterparts; (2) RNAi suppression of ascorbate oxidase (AO) resulted in a significant increase of AsA accumulation (0.86 μmol/g FW) in apoplast; (3) both mitochondrion-target and none-target overexpression of L-Galactose dehydrogenase (GalDH) did not significantly promote AsA production compared with WT (1.96 μmol/g), however a dramatic enhancement was observed following infiltration with L-galactono-1, 4-lactone (L-GalL), both in transgenic and WT plants. The best line produced AsA with the content of 3.90 μmol/g FW, which was about 2-fold of that in the untreated control (1.99 μmol/g FW). This study provides new strategies including GalPPase overexpression, AO suppression as well as L-GalL feeding for modern breeding aimed at stimulating the AsA content in plants.

Effects of Plant Secondary Metabolites(flavone) on Foraging Behavior of Sheep

Ling Wang,Deli Wang,Xiao Shun,Guowei Chen 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

Ingestion of plant secondary metabolites (PSMs) presents a major challenge to mammalian herbivores. We investigated the relationship between PSM concentrations in the diet and feeding patterns in sheep fed diet containing flavone extracted from Apocynum venetum. Results showed that the flavone concentrations significantly influenced food intake by sheep. With the concentration of flavone in diet increase, sheep can regulate their intake of PSM within limits that are tolerable.

Covalent Immobilization of Penicillin G Acylase onto Fe₃O₄@Chitosan Magnetic Nanoparticles

( Xiao Min Ling ),( Xiang Yu Wang ),( Ping Ma ),( Yi Yang ),( Jie Mei Qin ),( Xue Jun Zhang ),( Ye Wang Zhang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.5

Penicillin G acylase (PGA) was immobilized on magnetic Fe₃O₄@chitosan nanoparticles through the Schiff base reaction. The immobilization conditions were optimized as follows: enzyme/support 8.8 mg/g, pH 6.0, time 40 min, and temperature 25℃. Under these conditions, a high immobilization efficiency of 75% and a protein loading of 6.2 mg/g-support were obtained. Broader working pH and higher thermostability were achieved by the immobilization. In addition, the immobilized PGA retained 75% initial activity after ten cycles. Kinetic parameters Vmax and Km of the free and immobilized PGAs were determined as 0.113 mmol/min/mg-protein and 0.059 mmol/min/mg-protein, and 0.68 mM and 1.19 mM, respectively. Synthesis of amoxicillin with the immobilized PGA was carried out in 40% ethylene glycol at 25℃ and a conversion of 72% was obtained. These results showed that the immobilization of PGA onto magnetic chitosan nanoparticles is an efficient and simple way for preparation of stable PGA.

IMPACT OF COINFECTION WITH Vibrio parahaemolyticus AND WHITE SPOT SYNDROME VIRUS (WSSV) ON THE SURVIVAL OF PENAEID SHRIMP Litopenaeus vannamei

SONG Xiao-ling,ZHANG Xiao-jing,HUANG Jie,WANG Chun-di 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

中韓民衆間的相互認識以及好感度影響因素

왕효령 ( Wang Xiao Ling ) 서강대학교 동아연구소 2012 東亞 硏究 Vol.31 No.2

This study is based on two questionnaire surveys. It compares the China- Korean mutual understanding and impressions of each other, and analyzes the factors which influence the impressions. The study finds that the Korean surveyed consider that China is a backward country in many aspects, and that it is difficult to trust China and the Chinese. Moreover, the study shows that the socialist China needs to be more open, all of which worsens the public impression of China. But the Korean polled say that China develops quickly, and they feel good of Chinese cultures, which improve their impression of China. The Chinese surveyed consider Korea a leading country in many aspects, which improves the good impression of Korea. While praising the Korean cultural industries, the Chinese polled think that the Korean traditional cultures have been deeply impacted by Chinese cultures, and are very proud of their own traditional cultures. They also think that the Korean have a very strong sense of national pride, and such strong nationalism has the negative impact on the impression.

Causes, Features, and Outcomes of Drug-Induced Liver Injury in 69 Children from China

( Yun Zhu ),( Yong Gang Li ),( Jia Bo Wang ),( Shu Hong Liu ),( Li Fu Wang ),( Yan Ling Zhao ),( Yun Feng Bai ),( Zhong Xia Wang ),( Jian Yu Li ),( Xiao He Xiao ) 대한소화기학회 2015 Gut and Liver Vol.9 No.4

Background/Aims: Drug-induced liver injury (DILI) is a frequent cause of pediatric liver disease; however, the data on DILI are remarkably limited. Methods: All 69 children hospitalized with DILI between January 2009 and December 2011 were retrospectively studied. Results: A total of 37.7% of the children had medical histories of respiratory infection. The clinical injury patterns were as follows: hepatocellular 89.9%, cholestatic 2.9%, and mixed 7.2%. Liver biopsies from 55 children most frequently demonstrated chronic (47.3%) and acute (27.3%) hepatitis. Hypersensitivity features, namely, fever (31.9%), rash (21.7%), and eosinophilia (1.4%), were found. Twenty-four children (34.8%) developed chronic DILI. Antibiotics (26.1%) were the most common Western medicines (WMs) causing DILI, and the major implicated herbs were Ephedra sinica and Polygonum multiflorum. Compared with WM, the children whose injuries were caused by Chinese herbal medicine (CHM) showed a higher level of total bilirubin (1.4 mg/dL vs 16.6 mg/dL, p=0.004) and a longer prothrombin time (11.8 seconds vs 17.3 seconds, p=0.012), but they exhibited less chronic DILI (2/15 vs 18/39, p=0.031). Conclusions: Most cases of DILI in children are caused by antibiotics or CHM used to treat respiratory infection and present with hepatocellular injury. Compared with WM, CHM is more likely to cause severe liver injury, but liver injury caused by CHM is curable. (Gut Liver 2015;9:525-533)

Overexpression of TRPM7 is Associated with Poor Prognosis in Human Ovarian Carcinoma

Wang, Jing,Xiao, Ling,Luo, Chen-Hui,Zhou, Hui,Hu, Jun,Tang, Yu-Xi,Fang, Kai-Ning,Zhang, Yi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Background: The melastatin-related transient receptor potential 7 channel (TRPM7) is a nonselective cation channel that has been shown to promote tumor metastasis and progression. In this study, we determined the expression of TRPM7 in ovarian carcinomas and investigated its possible prognostic value. Materials and Methods: Samples were collected from 138 patients with ovarian cancer. Expression of TRPM7 was assessed by real-time PCR and immunohistochemistry, expressed with reference to an established scoring system and related to clinical pathological factors. Kaplan-Meier survival analysis was applied to estimate disease-free survival (DFS) and overall survival (OS). Univariate and multivariate cox regression analyses were performed to correlate TRPM7 expression levels with DFS and OS. Results: TRPM7 was highly expressed in ovarian carcinoma and significantly associated with decreased disease-free survival (DFS: median 20 months vs. 42 months, P=0.0002) and overall survival (OS: median 27 months vs. 46 months, P<0.001). Conclusion: Overexpression of TRPM7 expression is significantly associated with poor prognosis in patients with ovarian cancer.

Nanoparticle Realgar Powders Induce Apoptosis in U937 Cells through Caspase MAPK and Mitochondrial Pathways

Wang, Xiao-bo,Gao, Hui-Yuan,Hou, Bai-ling,Huangi, Jian,Xi, Rong-gang,Wu, Li-Jun 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5

School of Traditional Chinese Medicines, Shenyang Pharmaceutical University , Department of Pharmacy, Department of traditional Chinese MeNanoparticle realqar powders (NRP) inhibited U937 cell growth in a time and dose-dependent manner. U937 cells treated with NRP showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Caspase family inhibitor (z-VAD-fmk), caspase-8, -9 inhibitor (z-IETD-fmk, Ac-LEHD-CHO, respectively) and caspase-3 inhibiter (z-DEVD-fmk) partially prevented NRP -induced apoptosis. Moreover, the classical substrates of caspase-3 poly-ADP ribose polymerase (PARP) was degraded after U937 cells treatment with NRP. In audition, NRP Increased the ratio of Bax/Bcl-2 protein expression. Although p38 inhibitor (SB203580) and ERK inhibitor (PD98059) failed to block cell death, JNK inhibitor(SP600125) had marked inhibitory effects on NRP -induced apoptosis. Furthermore, the phosphorylation of JNK was up-regulated, suggesting that JNK was responsible for NRP -induced apoptosis in U937 cells. These results suggested that the caspase, mitochondria and MAPK signal pathways were involved in NRP-induced U937 apoptosis.