An Efficient Identification of 68 Apple Cultivars Using a Cultivar Identification Diagram (CID) Strategy and RAPD Markers

Wenyan Wang,Kun Wang,Fengzhi Liu,Jinggui Fang 한국원예학회 2012 원예과학기술지 Vol.30 No.5

The study aimed to establish an efficient tool for cultivar identification and characterization being the first steps of apple introduction and improvement program. We utilized a method to efficiently record DNA molecular fingerprints of plant individuals genotyped by RAPD, which could be used as efficient reference information for quick plant identification. Ten of sixty 11-mer primers were screened to identify the 68 apple genotypes which could be distinguished by a combination of several primers. All cultivars were easily identified by the corresponding primers marked on the cultivar identification diagram (CID). The results indicated that the CID strategy developed and employed in the apple cultivar identification could be vital in the utilization of DNA marker in other plants as well as the development of the apple industry.

A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola

Liu, Na,Jiang, Shijun,Feng, Songli,Shang, Wenyan,Xing, Guozhen,Qiu, Rui,Li, Chengjun,Li, Shujun,Zheng, Wenming The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.2

A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and ${\beta}$-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T. basicola and control samples. The results showed that the target pathogens could be detected from diseased tobacco plants by a combination of the specific primers. The sensitivity limitation was $100fg/{\mu}l$ of pure genomic DNA of the pathogens. This new assay can be applied to screen out target pathogens rapidly and reliably in one PCR and will be an important tool for the identification and precise early prediction of these two destructive diseases of tobacco.

A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola

Na Liu,Shijun Jiang,Songli Feng,Wenyan Shang,Guozhen Xing,Rui Qiu,Chengjun Li,Shujun Li,Wenming Zheng 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.2

A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and β-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T. basicola and control samples. The results showed that the target pathogens could be detected from diseased tobacco plants by a combination of the specific primers. The sensitivity limitation was 100 fg/μl of pure genomic DNA of the pathogens. This new assay can be applied to screen out target pathogens rapidly and reliably in one PCR and will be an important tool for the identification and precise early prediction of these two destructive diseases of tobacco.

An Efficient Identification of 68 Apple Cultivars Using a Cultivar Identification Diagram (CID) Strategy and RAPD Markers

Wang, Wenyan,Wang, Kun,Liu, Fengzhi,Fang, Jinggui Korean Society of Horticultural Science 2012 원예과학기술지 Vol.30 No.5

The study aimed to establish an efficient tool for cultivar identification and characterization being the first steps of apple introduction and improvement program. We utilized a method to efficiently record DNA molecular fingerprints of plant individuals genotyped by RAPD, which could be used as efficient reference information for quick plant identification. Ten of sixty 11-mer primers were screened to identify the 68 apple genotypes which could be distinguished by a combination of several primers. All cultivars were easily identified by the corresponding primers marked on the cultivar identification diagram (CID). The results indicated that the CID strategy developed and employed in the apple cultivar identification could be vital in the utilization of DNA marker in other plants as well as the development of the apple industry.

Identification and Characterization of a Novel Antibacterial Peptide, Avian β-Defensin 2 from Ducks

Deying Ma,Ruiqin Wang,Wenyan Liao,Zongxi Han,Shengwang Liu 한국미생물학회 2009 The journal of microbiology Vol.47 No.5

In this study, a novel avian β-defensin (AvBD) was isolated from duck pancreas. The complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. Homology, characterization and comparison of the gene with AvBD from other avian species confirmed that it was duck AvBD2. The mRNA expression of the gene was analyzed in 17 tissues from 21-day-old ducks. AvBD2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed in the muscular stomach, small intestine, kidney, spleen, thymus, and bursa of Fabricius; and weakly expressed in skin. We produced and purified recombinant AvBD2 by expressing the gene in Escherichia coli. As expected, the recombinant peptide exhibited strong bactericidal properties against Bacillus cereus, Staphylococcus aureus, and Pasteurella multocida, and weak bactericidal properties against E. coli and Salmonella choleraesuis. In addition, the recombinant protein retained antimicrobial activity against S. aureus under different temperatures (range, -20°C to 100°C) and pH values (range, 3 to 12).

A quantitative method for detecting meat contamination based on specific polypeptides

Feng, Chaoyan,Xu, Daokun,Liu, Zhen,Hu, Wenyan,Yang, Jun,Li, Chunbao Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.9

Objective: This study was aimed to establish a quantitative detection method for meat contamination based on specific polypeptides. Methods: Thermally stable peptides with good responses were screened by high resolution liquid chromatography tandem mass spectrometry. Standard curves of specific polypeptide were established by triple quadrupole mass spectrometry. Finally, the adulteration of commercial samples was detected according to the standard curve. Results: Fifteen thermally stable peptides with good responses were screened. The selected specific peptides can be detected stably in raw meat and deep processed meat with the detection limit up to 1% and have a good linear relationship with the corresponding muscle composition. Conclusion: This method can be effectively used for quantitative analysis of commercial samples.

HDRE: Coverage Hole Detection with Residual Energy in Wireless Sensor Networks

Yun-zhou Zhang,Xiaohua Zhang,Wenyan Fu,Zeyu Wang,Honglei Liu 한국통신학회 2014 Journal of communications and networks Vol.16 No.5

Coverage completeness is an important indicator forquality of service in wireless sensor networks (WSN). Due to limitedenergy and diverse working conditions, the sensor nodes havedifferent lifetimes which often cause network holes. Most of the existingmethods expose large limitation and one-sidedness becausethey generally consider only one aspect, either coverage rate or energyissue. This paper presents a novel method for coverage holedetection with residual energy in randomly deployed wireless sensornetworks. By calculating the life expectancy of working nodesthrough residual energy, we make a trade-off between network repaircost and energy waste. The working nodes with short lifetimeare screened out according to a proper ratio. After that, the locationsof coverage holes can be determined by calculating the jointcoverage probability and the evaluation criteria. Simulation resultshows that compared to those traditional algorithms without considerationof energy problem, our method can effectively maintainthe coverage quality of repairedWSN while enhancing the life spanof WSN at the same time.

Drought stress reduces the feeding preference of Nilaparvata lugens due to the accumulation of abscisic acid and callose deposition in rice

Liao Guangrong,Li Jitong,Fan Wenyan,Chen Lin,Liu Jinglan 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

The incidence of drought stress in plants has been increasing due to global warming, and the phytohormone abscisic acid (ABA) induces the formation of physical barriers in plants, such as callose accumulation. The brown planthopper (BPH; Nilaparvata lugens Stål) occurs throughout Asia and feeds on rice, but the effects of drought stress on BPH feeding remain unclear. In this study, we observed changes in callose formation and ABA content in rice during drought stress. ABA content and the relative expression of ABA synthesis genes OsNCED3 and OsNCED5 were higher in drought-stressed rice than the non-stressed control. Similarly, the expression levels of callose synthesis genes and callose deposition were significantly higher in drought-stressed rice as compared to non-stressed plants, and this impacted BPH feeding. Our results indicated that rice resistance to BPH increased during drought stress due to the accumulation of callose and increasing ABA levels. Our findings provide a basis for understanding BPH feeding performance on rice during drought stress and offer novel insights relative to control during periods of water shortage.

Effect of Ligustilide Supplement on Physical Fitness of Athletes

Xiaojuan Gao,Shuai Yang,Meng Liu,Yawen Jiang,Li Wenyan,Rui Ma,Zijian Zhao,Xuelin Zhang 아시아건강운동학회 2020 Journal of Asian Society for Health & Exercise Vol.2 No.2

PURPOSE: In this study, high content ligustilide plant extract was supplemented to monitor the changes of physical fitness and physiological function of athletes, and to explore the effect of ligustilide plant extract on physical function and physical fitness of athletes. METHODS: Thirty five high-level basketball players from Zhengzhou University were selected and divided into two groups randomly: nutritional supplement group (S, n = 18) and control group (C, n=17). This study was approved by the Ethics Committee of Zhengzhou University. Informed consent was obtained from each subject. The intervention period was 8 weeks, six days per week. The training method adopted the small cycle plate training scheme. We measured changes of the scores of rating of perceived exertion (RPE), physical fitness test (15m running, 30m running, 400m running) and routine blood test (mainly including red blood cell count, hemoglobin concentration and average erythrocyte hemoglobin concentration, etc.) before and after training. RESULTS: In group S, the percentage of RPE (grade 6-11) and RPE (grade 12-18) before experiment was 37.5% and 62.5%. After 8 weeks of experiment, 62.5% of RPE (grade 6-11) and 37.5% of RPE (grade 12-18). In group C, 14% of RPE (grade 6-11), 86% of slight RPE (grade 12-18), and 100% of RPE (grade 12-18) after 8 weeks of experiment. It was found that after 8 weeks of training, the number of red blood cells, the concentration of hemoglobin and the average concentration of red blood cell hemoglobin in group S were significantly higher than those before the experiment (p <0.05). According to the comparison of the physical fitness test results of the athletes in group S, it was found that the performance of 400m after 4 weeks and 8 weeks was significantly higher compared with pre-experiment (p<0.05). The changes of 30m and 15m were not significant different (p>0.05). The 400-meter test performance of athletes in group C was significantly higher than that before the experiment only after 8 weeks of training (p<0.05). CONCLUSIONS: Ligustilide supplementation can increase the number of red blood cell and hemoglobin concentration of athletes and has a positive impact on the physical fitness of athletes and the recovery of fatigue.

CacheSCDefender: VMM-based Comprehensive Framework against Cache-based Side-channel Attacks

( Chao Yang ),( Yunfei Guo ),( Hongchao Hu ),( Wenyan Liu ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.12

Cache-based side-channel attacks have achieved more attention along with the development of cloud computing technologies. However, current host-based mitigation methods either provide bad compatibility with current cloud infrastructure, or turn out too application-specific. Besides, they are defending blindly without any knowledge of on-going attacks. In this work, we present CacheSCDefender, a framework that provides a (Virtual Machine Monitor) VMM-based comprehensive defense framework against all levels of cache attacks. In designing CacheSCDefender, we make three key contributions: (1) an attack-aware framework combining our novel dynamic remapping and traditional cache cleansing, which provides a comprehensive defense against all three cases of cache attacks that we identify in this paper; (2) a new defense method called dynamic remapping which is a developed version of random permutation and is able to deal with two cases of cache attacks; (3) formalization and quantification of security improvement and performance overhead of our defense, which can be applicable to other defense methods. We show that CacheSCDefender is practical for deployment in normal virtualized environment, while providing favorable security guarantee for virtual machines.