Enhancement of Scenedesmus sp. LX1 Biomass Production and Lipid Accumulation Using Iron in Artificial Wastewater and Domestic Secondary Effluent

( Wen Yu Zhao ),( Jun Yi Yu ),( Yin Hu Wu ),( Yu Hong ),( Hong Ying Hu ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.2

While coupling wastewater treatment with microalgal bioenergy production is very promising, new approaches are needed to enhance microalgal growth and lipid accumulation in wastewater. Therefore, this study investigated the effect of iron on the growth, nutrient removal, and lipid accumulation of Scenedesmus sp. LX1 in both artificial wastewater and domestic secondary effluents. When increasing the iron concentration from 0 to 2 mg/l in the artificial wastewater, the biomass production of Scenedesmus sp. LX1 increased from 0.17 to 0.54 g/l; the nitrogen and phosphorus removal efficiency increased from 15.7% and 80.6% to 97.0% and 99.2%, respectively; and the lipid content was enhanced 84.2%. The relationship between the carrying capacity/maximal population growth rate of Scenedesmus sp. LX1 and the initial iron concentration were also in accordance with the Monod model. Furthermore, when increasing the iron concentration to 2 mg/l in four different domestic secondary effluent samples, the lipid content and lipid production of Scenedesmus sp. LX1 was improved by 17.4-33.7% and 21.5-41.8%, respectively.

An Empirical Analysis of Production Efficiency of Vegetable Industry in Hebei Province

Shuang-Yu Hu(호쌍우),Sung-Chan Kim(김성찬),Yu-Cong Sun(손우총),Zhi-Run Li(이지윤),Wen Ju(극문),Shi-Yong Piao(박세영),Jong-In Lee(이종인) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.8

중국 허베이성의 채소 산업의 발전은 이미 농민의 소득 증대를 촉진하는 중요한 경로로 되고 있으며 생태환경 오염, 자연 자원 부족 등 문제가 날로 심각해지는 상황에서 채소 산업의 고품질 발전을 실현하기 위해서는 반드시 채소산업의 생산효율을 제고함으로써 실현해야 한다. 농가의 생산성 제고를 위한 효율성 측정이 중요하다. 특히 효율성 계측시 농가의 자본투입, 토지투입 등 현실적 투입 고려가 필요하다. 본 연구는 국내외 연구 성과를 바탕으로 허베이성 2011-2018 채소 생산 투입량, 산출량 데이터, 맴퀴스트 지수와 DEA 모델을 바탕으로 허베이성 산업의 생산 효율성을 연구하였다. 효율성 계측 결과를 이용하여 비효율성의 원인을 규명하고, 농가 효율성 향상을 위한 대안을 제시한다. 분석을 통해 기술 진보 요인이 허베이성의 채소 생산능률의 증가에 영향을 미치는 중요한 원인이라는 것을 발견했다. 연구결과에 따라 본 연구는 채소 생산기술 수준을 제고하는 목적에 기초하여 허베이성 채소 생산의 구체적인 조건과 결합하여 채소 생산기술의 개발과 응용, 기계화 생산, 집약화 경영 발전 등 방면에서 상응하는 대책과 건의를 제기할 것이다. The development of the vegetable industry in Hebei province has become an important way to increase a farmer"s income. Under the background of increasingly serious problems, such as environmental pollution and the shortage of natural resources, it is necessary to improve the production efficiency of the vegetable industry to achieve high-quality development of the vegetable industry. Based on collating research results at home and abroad, this study selected the 2011-2018 vegetable production input and output data of Hebei Province. The production efficiency of Hebei Province"s industry was studied based on the Malmquist index and DEA model. The analysis showed that technological progress is an important factor in improving the vegetable production efficiency in Hebei Province. Based on the research results, the level of vegetable production technology, combined with the specific conditions of vegetable production in Hebei Province, can be improved by developing and applying vegetable production technology, mechanized production, and intensive management development.

Enhancement of Scenedesmus sp. LX1 Biomass Production and Lipid Accumulation Using Iron in Artificial Wastewater and Domestic Secondary Effluent

Zhao, Wen-Yu,Yu, Jun-Yi,Wu, Yin-Hu,Hong, Yu,Hu, Hong-Ying The Korean Society for Microbiology and Biotechnol 2014 한국미생물·생명공학회지 Vol.42 No.2

While coupling wastewater treatment with microalgal bioenergy production is very promising, new approaches are needed to enhance microalgal growth and lipid accumulation in wastewater. Therefore, this study investigated the effect of iron on the growth, nutrient removal, and lipid accumulation of Scenedesmus sp. LX1 in both artificial wastewater and domestic secondary effluents. When increasing the iron concentration from 0 to 2 mg/l in the artificial wastewater, the biomass production of Scenedesmus sp. LX1 increased from 0.17 to 0.54 g/l; the nitrogen and phosphorus removal efficiency increased from 15.7% and 80.6% to 97.0% and 99.2%, respectively; and the lipid content was enhanced 84.2%. The relationship between the carrying capacity/maximal population growth rate of Scenedesmus sp. LX1 and the initial iron concentration were also in accordance with the Monod model. Furthermore, when increasing the iron concentration to 2 mg/l in four different domestic secondary effluent samples, the lipid content and lipid production of Scenedesmus sp. LX1 was improved by 17.4-33.7% and 21.5-41.8%, respectively.

Metagenomics analysis of the eff ects of long-term stand age on benefi cial soil bacterial community structure under Chinese ancient mulberry farming practice

Yu Cui,Li Yong,Mo Rongli,Deng Wen,Zhu Zhixian,Hu Xingming 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.6

The ecological distribution and activities of soil microbes can refl ect the infl uence of environmental factors on microbes andchanges in soil fertility. Understanding the community structure of the soil microbes is of great importance to maintaining soilhealth in ancient mulberry plantation ecosystems. In this study, the population structure of soil microorganisms in diff erentages of mulberry plantations was analyzed using high-throughput sequencing technology. A total of 1616, 1568, 1379 and1477 operational taxonomic units were acquired from samples of 3Y (3-year-old), 35Y (35-year-old), 200Y (200-year-old)and 600Y (600-year-old) soils, respectively. The results exhibited that the highest community diversity was found in 35Ysoil, followed by 200Y soil, and showed that the stand age strongly infl uenced the community structure of soil bacteria. Atotal of 23 phyla were detected in 12 samples, and the genus of bacteria that predominated the diff erent stand ages mainlyincluded; Phenylobacterium , Cohnella , Lactococcus , Sphingomonas , Bacillus , and Microbispora . The relative abundanceof Phenylobacterium accounts for 22.6–53.5% of the total abundance. This study showed that the richness and diversity ofthe soil bacteria decreased as the stand age increased in ancient mulberry plantations.

A unique genetic lineage at the southern coast of China in the agar-producing Gracilaria vermiculophylla (Gracilariales, Florideophyceae)

Hu, Zi-Min,Liu, Ruo-Yu,Zhang, Jie,Duan, De-Lin,Wang, Gao-Ge,Li, Wen-Hong The Korean Society of Phycology 2018 ALGAE Vol.33 No.3

Ocean warming can have significant negative impacts on population genetic diversity, local endemism and geographical distribution of a wide range of marine organisms. Thus, the identification of conservation units with high risk of extinction becomes an imperative task to assess, monitor, and manage marine biodiversity for policy-makers. Here, we surveyed population structure and genetic variation of the red seaweed Gracilaria vermiculophylla along the coast of China using genome-based amplified fragment length polymorphism (AFLP) scanning. Regardless of analysis methods used, AFLP consistently revealed a south to north genetic isolation. Populations at the southern coast of China showed unique genetic variation and much greater allelic richness, heterozygosity, and average genetic diversity than the northern. In particular, we identified a geographical barrier that may hinder genetic exchange between the two lineages. Consequently, the characterized genetic lineage at the southern coast of China likely resulted from the interplay of post-glacial persistence of ancestral diversity, geographical isolation and local adaptation. In particular, the southern populations are indispensable components to explore evolutionary genetics and historical biogeography of G. vermiculophylla in the northwestern Pacific, and the unique diversity also has important conservation value in terms of projected climate warming.

Suppression of Ku80 Correlates with Radiosensitivity and Telomere Shortening in the U2OS Telomerase-negative Osteosarcoma Cell Line

Hu, Liu,Wu, Qin-Qin,Wang, Wen-Bo,Jiang, Huan-Gang,Yang, Lei,Liu, Yu,Yu, Hai-Jun,Xie, Cong-Hua,Zhou, Yun-Feng,Zhou, Fu-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Ku70/80 heterodimer is a central element in the nonhomologous end joining (NHEJ) DNA repair pathway, Ku80 playing a key role in regulating the multiple functions of Ku proteins. It has been found that the Ku80 protein located at telomeres is a major contributor to radiosensitivity in some telomerase positive human cancer cells. However, in ALT human osteosarcoma cells, the precise function in radiosensitivity and telomere maintenance is still unknown. The aim of this study was to investigate the effects of Ku80 depletion in the U2OS ALT cell line cell line. Suppression of Ku80 expression was performed using a vector-based shRNA and stable Ku80 knockdown in cells was verified by Western blotting. U2OS cells treated with shRNA-Ku80 showed lower radiobiological parameters (D0, Dq and SF2) in clonogenic assays. Furthermore, shRNA-Ku80 vector transfected cells displayed shortening of the telomere length and showed less expression of TRF2 protein. These results demonstrated that down-regulation of Ku80 can sensitize ALT cells U2OS to radiation, and this radiosensitization is related to telomere length shortening.

The Numerical Study of the Air Lubrication on the Hull Bottom of the Ship

Hu Shi-liang,Zhen An-ran,Sun Wen-yu,Yang Li,Wei Jin-fang 한국전산유체공학회 2022 한국전산유체공학회 학술대회논문집 Vol.2022 No.10

A Convenient Method for the Enantiomeric Separation of α-Amino Acid Esters as Benzophenone Imine Schiff Base Derivatives

Hu Huang,Wonjae Lee,Wen Jun Xu,Jing-Yu Jin,Joon Hee Hong,신현재 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.6

A convenient liquid chromatographic method for the separation of α-amino acid esters as benzophenone Schiff base derivatives on coated chiral stationary phases (CSPs) (Chiralcel OD-H, Chiralcel OD, Chiralpak AD-H, Chiralpak AD, and Chiralpak AS) or covalently immobilized CSPs (Chiralpak IA, Chiralpak IB, and Chiralpak IC) derived from polysaccharide derivatives is described. Benzophenone imine derivatives of α-amino acid esters were readily prepared by stirring benzophenone imine and the hydrochloride salts of α-amino acid esters in 2-propanol. The chromatographic separations were conducted at a flow rate 1.0 mL/min and a detection wavelength of 254 nm; 0.5% 2-propanol/hexane (v/v) was used on CSPs. In general, the resolution of Chiralpak IC was superior to those of the other CSPs. In addition, the resolutions of other arylimine derivatives of α-amino acid esters and the effects of different mobile phases on the enantiomeric separation of α-amino acid esters as benzophenone imine derivatives on Chiralpak IC were investigated.

Professionals’ experiences and attitudes toward use of Traditional Chinese Medicine in hospice palliative inpatient care units: A multicenter survey in Taiwan

Yu-Jia Lin,Hsiao-Ting Chang,Ming-Hwai Lin,Ru-Yih Chen,Ping-Jen Chen,Wen-Yuan Lin,Jyh-Gang Hsieh,Ying-Wei Wang,Chung-Chieh Hu,Yi-Sheng Liou,Tai-Yuan Chiu,Chun-Yi Tu,Yi-Jen Wang,Bo-Ren Cheng,Tzeng-Ji Ch 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.2

Background: Medical staff may have difficulties in using conventional medicine to manage symptoms among terminally ill patients, including adverse effects of the treatment. Traditional Chinese medicine (TCM) is regarded as a complementary or alternative medicine, and has been increasingly used in the field of palliative medicine in recent years. This study aimed to investigate the experiences of and attitudes toward using TCM among palliative care professionals, and to provide preliminary information about its use in palliative care. Methods: This was a cross-sectional survey study conducted in eight inpatient hospice wards in Taiwan between December 2014 and February 2016. The questionnaire was self-administered, and was analyzed with descriptive statistics including Pearson’s Chi-square test and Fisher’s exact test. Results: A total of 251 palliative care professionals responded to the questionnaire, of whom 89.7% and 88.9% believed that the use of TCM could improve the physical symptoms and quality of life in terminally ill patients, respectively. Overall, 59.8%, of respondents suggested that TCM had rare side effects, and 58.2% were worried that TCM could affect the liver and kidney function of patients. In total, 89.7% and 88.0% of professionals agreed there were no suitable clinical practice guidelines and educational programs, respectively, for TCM use in palliative care. Conclusions: Most of the respondents agreed there was insufficient knowledge, skills-training, and continuing education on the use of TCM in terminally ill patients in Taiwan. These results show that to address patient safety considerations, guidelines about use of TCM in palliative care should be established.

Prevalence, Risk Factors, and Psychological Effects of Overactive Bladder in Chinese University Students

Yu Liang,Guo Wei Si,Hui Jie Hu,Zhen Wei Zhang,Cui Ping Song,Qi Feng Dou,Jian Guo Wen 대한배뇨장애요실금학회 2022 International Neurourology Journal Vol.26 No.4

Purpose: The purpose of this study was to investigate the prevalence and risk factors of overactive bladder (OAB) in young adults and to explore the influence of OAB on mental health. Methods: Between October 2019 and January 2020, 14,010 anonymous questionnaires were distributed to freshmen at 2 universities in Henan, China. The students came from all over the country. The questionnaire included general items and information necessary to calculate the overactive bladder symptom score, the Chinese version of the Pittsburgh Sleep Quality Index (PSQI) score, Self-Esteem Scale (SES) score, and Self-Rating Depression Scale (SDS) score. The relationships between the prevalence of OAB and its risk factors were evaluated. Results: The overall prevalence of OAB was 6.0%, with 4.3% of participants characterized as having dry OAB and 1.7% as having wet OAB. The prevalence of mild OAB was 5.5%, and that of moderate OAB was 0.5%; no severe OAB was observed. Higher prevalence rates of OAB were found among women, respondents with constipation, and respondents with primary nocturnal enuresis (PNE) (P <0.05). Compared to healthy controls, the OAB group exhibited a higher mean SDS score (52.12±8.986 vs. 47.71±9.399, P<0.001) and mean PSQI score (5.28±2.486 vs. 4.27±2.431, P<0.001), but a lower mean SES score (27.78±3.599 vs. 29.57±4.109, P<0.001). Conclusions: OAB significantly affects the mental health of young adults. Female sex, constipation, and PNE are risk factors for OAB.