http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Cui, Wan Shou,Kim, Sang Deuk,Choi, Kyung Soo,Zhao, Chen,Park, Jong Kwan Elsevier 2009 JOURNAL OF SEXUAL MEDICINE Vol.6 No.6
<P>Simultaneous urethral repair and reimplantation of penile prosthesis in a patient with urethral stricture induced by rotated tubing of a three piece penile prosthesis has not been reported yet.</P>
Cui, Chang-Hao,Liu, Qing-Mei,Kim, Jin-Kwang,Sung, Bong-Hyun,Kim, Song-Gun,Kim, Sun-Chang,Im, Wan-Taek American Society for Microbiology 2013 Applied and environmental microbiology Vol.79 No.19
<P>Here, we isolated and characterized a new ginsenoside-transforming β-glucosidase (BglQM) from <I>Mucilaginibacter</I> sp. strain QM49 that shows biotransformation activity for various major ginsenosides. The gene responsible for this activity, <I>bglQM</I>, consists of 2,346 bp and is predicted to encode 781 amino acid residues. This enzyme has a molecular mass of 85.6 kDa. Sequence analysis of BglQM revealed that it could be classified into glycoside hydrolase family 3. The enzyme was overexpressed in <I>Escherichia coli</I> BL21(DE3) using a maltose binding protein (MBP)-fused pMAL-c2x vector system containing the tobacco etch virus (TEV) proteolytic cleavage site. Overexpressed recombinant BglQM could efficiently transform the protopanaxatriol-type ginsenosides Re and Rg<SUB>1</SUB> into (<I>S</I>)-Rg<SUB>2</SUB> and (<I>S</I>)-Rh<SUB>1</SUB>, respectively, by hydrolyzing one glucose moiety attached to the C-20 position at pH 8.0 and 30°C. The <I>K<SUB>m</SUB></I> values for <I>p</I>-nitrophenyl-β-<SMALL>d</SMALL>-glucopyranoside, Re, and Rg<SUB>1</SUB> were 37.0 ± 0.4 μM and 3.22 ± 0.15 and 1.48 ± 0.09 mM, respectively, and the <I>V</I><SUB>max</SUB> values were 33.4 ± 0.6 μmol min<SUP>−1</SUP> mg<SUP>−1</SUP> of protein and 19.2 ± 0.2 and 28.8 ± 0.27 nmol min<SUP>−1</SUP> mg<SUP>−1</SUP> of protein, respectively. A crude protopanaxatriol-type ginsenoside mixture (PPTGM) was treated with BglQM, followed by silica column purification, to produce (<I>S</I>)-Rh<SUB>1</SUB> and (<I>S</I>)-Rg<SUB>2</SUB> at chromatographic purities of 98% ± 0.5% and 97% ± 1.2%, respectively. This is the first report of gram-scale production of (<I>S</I>)-Rh<SUB>1</SUB> and (<I>S</I>)-Rg<SUB>2</SUB> from PPTGM using a novel ginsenoside-transforming β-glucosidase of glycoside hydrolase family 3.</P>
Cui, Chang-Hao,Kim, Sun-Chang,Im, Wan-Taek Springer International 2013 Applied microbiology and biotechnology Vol.97 No.2
<P>This study focused on the cloning, expression, and characterization of ginsenoside-transforming recombinant β-glucosidase from Actinosynnema mirum KACC 20028(T) in order to biotransform ginsenosides efficiently. The gene, termed as bglAm, encoding a β-glucosidase (BglAm) belonging to the glycoside hydrolase family 3 was cloned. bglAm consisted of 1,830 bp (609 amino acid residues) with a predicted molecular mass of 65,277 Da. This enzyme was overexpressed in Escherichia coli BL21(DE3) using a GST-fused pGEX 4T-1 vector system. The recombinant BglAm was purified with a GSTbind agarose resin and characterized. The optimum conditions of the recombinant BglAm were pH 7.0 and 37 C. BglAm could hydrolyze the outer and inner glucose moieties at the C3 and C20 of the protopanaxadiol-type ginsenosides (i.e., Rb(1) and Rd, gypenoside XVII) to produce protopanaxadiol via gypenoside LXXV, F(2), and Rh(2)(S) with various pathways. BglAm can effectively transform the ginsenoside Rb(1) to gypenoside XVII and Rd to F(2); the K (m) values of Rb(1) and Rd were 0.69??0.06 and 0.45??0.02 mM, respectively, and the V (max) values were 16.13??0.29 and 51.56??1.35 μmol min(-1) mg(-1) of protein, respectively. Furthermore, BglAm could convert the protopanaxatriol-type ginsenoside Re and Rg(1) into Rg(2)(S) and Rh(1)(S) hydrolyzing the attached glucose moiety at the C6 and C20 positions, respectively. These various ginsenoside-hydrolyzing pathways of BglAm may assist in producing the minor ginsenosides from abundant major ginsenosides.</P>
Cui, Chang-Hao,Fu, Yaoyao,Jeon, Byeong-Min,Kim, Sun-Chang,Im, Wan-Taek The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.6
Background: The separation of isomeric compounds from a mixture is a recurring problem in chemistry and phytochemistry research. The purification of pharmacologically active ginsenoside Rb<sub>3</sub> from ginseng extracts is limited by the co-existence of its isomer Rb<sub>2</sub>. The aim of the present study was to develop an enzymatic elimination-combined purification method to obtain pure Rb<sub>3</sub> from a mixture of isomers. Methods: To isolate Rb<sub>3</sub> from the isomeric mixture, a simple enzymatic selective elimination method was used. A ginsenoside-transforming glycoside hydrolase (Bgp2) was employed to selectively hydrolyze Rb<sub>2</sub> into ginsenoside Rd. Ginsenoside Rb<sub>3</sub> was then efficiently separated from the mixture using a traditional chromatographic method. Results: Chromatographic purification of Rb<sub>3</sub> was achieved using this novel enzymatic elimination-combined method, with 58.6-times higher yield and 13.1% less time than those of the traditional chromatographic method, with a lower minimum column length for purification. The novelty of this study was the use of a recombinant glycosidase for the selective elimination of the isomer. The isolated ginsenoside Rb<sub>3</sub> can be used in further pharmaceutical studies. Conclusions: Herein, we demonstrated a novel enzymatic elimination-combined purification method for the chromatographic purification of ginsenoside Rb<sub>3</sub>. This method can also be applied to purify other isomeric glycoconjugates in mixtures.
Yizao Wan,Zhonghong Lin,Deqiang Gan,Teng Cui,Meirong Wan,Fanglian Yao,Quanchao Zhang,Honglin Luo 한국섬유공학회 2019 Fibers and polymers Vol.20 No.8
Graphene-based nanomaterials have been used as biomaterials to enhance cell adhesion, growth, anddifferentiation. However, the effect of graphene materials on cancer cell behavior has not been thoroughly investigated. Herein, we have incorporated graphene oxide (GO) into cellulose acetate (CA) to develop nanofibrous scaffolds for in vitrocancer cell culture, which is a crucial step for drug screening and cancer research. The GO/CA scaffolds were seeded withbreast cancer cells and cell viability, proliferation, adhesion, infiltration, and morphology were assessed. Mechanicalcharacterization demonstrated that the mechanical properties of GO/CA scaffolds were significantly better than bare CAscaffold and improved with increasing GO content. More importantly, the in vitro cell studies showed that the cancer cells onGO/CA scaffolds had significantly higher viability and better cell adhesion and growth than bare CA. Our results confirm animportant role of GO in improving mechanical properties and cancer cell performance on GO/CA scaffolds. These resultssuggest the potential of the GO/CA scaffolds as a promising candidate for in vitro cancer models.
Xue-fen Wan(만설분),Yi Yang(양의),Jian Cui(최검),Tao Zheng(정도),Li Ma(마려) 대한전자공학회 2012 전자공학회논문지 Vol.49 No.12
본 논문에서는 중국 현지 염색 기업의 열 센서망에 적용 가능한 원격 가상 현실 제어 플랫폼에서 실행할 수 있는 데이터베이스 액세스를 가상 현실 모델링 언어 (VRML)를 소개하였다. 또한, 3차원 실시간 자료 시각화를 위한 가상 현실 모델링 언어-액티브엑스 서버 페이지(VRML-ASP)를 소개하였다. 나아가 이와 관련된 스크립트도 다루었을 뿐만 아니라, 열 센서 노드와 센서 영역에서의 움직임을 분석하였다. 이 데이터베이스 프레임워크는 마이크로칩에서 발표한 마이와이<SUP>TM</SUP>(MiWi<SUP>TM</SUP>)의 표준에 최적화되었다. 이러한 분석 결과를 근거로, 시스템 구성에 따라 가상 현실 모델링 언어-액티브엑스 서버 페이지(VRML-ASP) 데이터 액세스 프레임워크가 가상 현실 원격 산업 공정 제어 시스템을 위한 하나의 경쟁력 있는 데이터 관리 해결책이 될 수 있음을 확인할 수 있었다. A Virtual Reality Modeling Language (VRML) database access in remote virtual reality control platform for dyeing enterprise MiWi<SUP>TM</SUP> thermal sensor network is presented in this paper. The VRML-ASP framework is introduced for 3D real-time data plotting in this application. The activities of thermal sensor nodes and sensor area are analyzed. The database access framework is optimized for MiWi<SUP>TM</SUP> wireless sensor networks. The experimental results show that VRML-ASP database access framework could be a reliable and competitive data-manage candidate for targeted virtual reality remote industrial visualization application.