Encapsulating doxorubicin-intercalated lamellar nanohydroxyapatite into PLGA nanofibers for sustained drug release

Honglin Luo,Yang Zhang,Zhiwei Yang,Guifu Zuo,Quanchao Zhang,Fanglian Yao,Yizao Wan 한국물리학회 2019 Current Applied Physics Vol.19 No.11

In this work, doxorubicin (DOX) was intercalated into layered nanohydroxyapatite (LHAp). The drug loaded LHAp (DOX@LHAp) was then mixed with poly(lactic-co-glycolic acid) (PLGA) and electrospun to yield DOX@ LHAp/PLGA composite scaffolds. As control, needle-like nanohydroxyapatite (nHAp) was also used to make an DOX@nHAp/PLGA composite scaffold and bare DOX was used to fabricate DOX/PLGA scaffold. The morphology, release behavior of DOX, and capability to inhibit cancer cells were assessed. The addition of DOXloaded nHAp to PLGA causes a slight decrease in the average fiber diameter of DOX@LHAp/PLGA as compared to PLGA. The in vitro drug release tests reveal a much faster release of DOX from DOX/PLGA than DOX@LHAp/ PLGA. Moreover, DOX@LHAp/PLGA displays a more sustainable release over DOX@nHAp/PLGA due to the storage of DOX in the gallery of LHAp, which is further proved by their cancer cell inhibition results. We believe that the DOX@LHAp/PLGA scaffold has potential as an implantable drug delivery system.

De novo genome assembly and annotation of Holothuria scabra (Jaeger, 1833) from nanopore sequencing reads

Luo Honglin,Huang Guanghua,Li Jianbin,Yang Qiong,Zhu Jiajie,Zhang Bin,Feng Pengfei,Zhang Yongde,Yang Xueming 한국유전학회 2022 Genes & Genomics Vol.44 No.12

Background: Holothuria scabra is a costly gourmet and traditional Chinese tonic medicine. However, the lack of high-quality genome information hinders the genetic, phylogenetic, and bioactivator researches. Objective: To construct high-quality genomic data of H. scabra and conduct genome-wide phylogenetic analysis. Methods: The whole genome of a male H. scabra was sequenced based on Nanopore MinION platform, and the sequence was assembled by wtdbg2. Transcriptome sequencing was used to aid the gene annotation. Repeat sequences, non-coding RNA, pseudogene and gene functional annotation were analyzed. 750 single-copy gene families from ten species were applied to construct phylogenetic tree for evolutionary analysis by using the ML method. Results: The H. scabra genome of 1.18 Gb (N50 = 1557,492 bp) with 500.42 Mb of putative repetitive sequences was assembled from a male H. scabra individual, and 16,642 protein-coding genes, 951 pseudogenes, 1791 motifs and 45,400 domains from the generated assembly were identified. The divergence time between H. scabra and its ancestor was estimated approximately 192.6 million years ago. H. scabra and A. japonicas joined together while sea urchin and sea star diverged about 440 Mya ago. Some key genes involved in notochord and gill slits development, skeleton degeneration and nervous system, as well as homeobox genes differ between H. scabra and Apostichopus japonicas. Conclusion: We report the first whole genome of H. scabra with expectation that this will be a valuable resource for genetic, phylogenetic, breading, molecular biology and bioactivator studies of sea cucumbers and other invertebrates.

Bacterial cellulose/graphene oxide nanocomposite as a novel drug delivery system

Honglin Luo,Haiyong Ao,Gen Li,Wei Li,Guangyao Xiong,Yong Zhu,Yizao Wan 한국물리학회 2017 Current Applied Physics Vol.17 No.2

Graphene-based nanocarriers not only possess large specific surface area but also prevent drugs from premature release outside the target cells. However, agglomeration in aqueous solution is a critical challenge. In this work, graphene oxide (GO) was uniformly embedded into the three-dimensional (3D) porous network of bacterial cellulose (BC) to form a novel BC/GO nanocomposite drug nanocarrier. For the first time, ibuprofen (IBU) was loaded onto the BC/GO nanocomposites. Scanning electron microscope (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) reveal the successful drug loading onto BC/GO nanocomposites. In vitro drug release studies indicate that the drug release of IBU@BC/GO follows a non-Fickian diffusion mechanism. Another important feature of this BC/GO nanocarrier is its better cell viability in comparison to BC. It is believed that this new nanocarrier is a potential choice for drug delivery system.

Enhanced Biological Behavior of Bacterial Cellulose Scaffold by Creation of Macropores and Surface Immobilization of Collagen

Guangyao Xiong,Yizao Wan,Honglin Luo,Chen Zhang,Yong Zhu 한국고분자학회 2015 Macromolecular Research Vol.23 No.8

Bacterial cellulose (BC) is considered a promising three-dimensional (3D) nanofibrous scaffold for tissue engineering. To further improve its biological behavior, BC scaffold was modified by the creation of macropores and the immobilization of collagen (COL) on the surface. The creation of macropores was performed by laser perforation technique and the immobilization of collagen was achieved by solution immersion and subsequent crosslinking. The asprepared macroporous BC/COL nanocomposite (denoted as mBC/COL) was characterized by SEM, FTIR, contact angle measurement, and dynamic mechanical analysis, and its cell behavior was evaluated by MTT assay. SEM and FTIR confirmed the presence of collagen coating and patterned macropores (300 μm). Although the presence of macropores and collagen reduced its storage modulus and hydrophilicity, mBC/COL exhibited sufficient stiffness and wettability. More importantly, preliminary cell studies demonstrated that mBC/COL exhibited improved biological activity over BC and BC/COL due to the co-existence of macropores and collagen.

Simultaneously depositing polyaniline onto bacterial cellulose nanofibers and graphene nanosheets toward electrically conductive nanocomposites

Yizao Wan,Jin Li,Zhiwei Yang,Haiyong Ao,Lingling Xiong,Honglin Luo 한국물리학회 2018 Current Applied Physics Vol.18 No.8

In this study, we report the construction of a ternary flexible nanocomposite of bacterial cellulose/graphene/polyaniline (BC/GE/PANI) via a facile two-step strategy. Bacterial cellulose/graphene (BC/GE) is first prepared by a novel in situ membrane-liquid-interface method, in which the three-dimensional continuous BC nanofibers can be maintained and the introduced GE can improve the mechanical properties mainly due to the uniform dispersion of GE in the BC matrix. To construct the effectively interconnected conductive paths between separated GE nanosheets, polyaniline (PANI) is simultaneously deposited on the surfaces of both BC nanofibers and GE nanosheets to obtain BC/GE/PANI with excellent electrical conductivity. It is found that the as-prepared BC/GE/PANI has an electrical conductivity of 1.7 ± 0.1 S cm−1, which is higher than most of PANI-based composites. It is believed that the BC/GE/PANI nanocomposite possesses great potential for applications in electromagnetic shielding and flexible electrodes.

Self-assembled magnetic lamellar hydroxyapatite as an efficient nanovector for gene delivery

Guangyao Xiong,Yizao Wan,Guifu Zuo,Kaijing Ren,Honglin Luo 한국물리학회 2015 Current Applied Physics Vol.15 No.7

Magnetic lamellar hydroxyapatite (ML-HA) nanoparticles were synthesized by a template-assisted selfassembly process. The as-prepared ML-HA nanoparticles self-assembled under different conditions were characterized by XRD, TEM, cytotoxicity assessment, and DNA-loading and transfection efficiency measurements. We found that the structure and morphology of ML-HA were controlled by self-assembly conditions. The ML-HA synthesized in this work exhibited good biocompatibility. The DNA-loading capacity and z-potential of ML-HA were much lower in comparison to bare lamellar HA (L-HA) without magnetic nanoparticles. Despite that, the ML-HA with good lamellar structure showed 47% higher transfection efficiency than L-HA. Results suggested that the ordered lamellar structure is a key factor in controlling transfection efficiency and magnetization is an effective way of improving the transfection efficiency of lamellar HA. Mechanisms were proposed to interpret these experimental results. It is demonstrated that the ML-HA may be a promising gene vector to deliver DNA into the cells effectively and safely.

Constructing Hierarchical Porous Bacterial Cellulose Outer Layer for Enhanced SMCs Infiltration in a Double-Layered Vascular Graft

Jie Wang,Weijuan Nie,Ming Wang,Chen Zhang,Jian Li,Honglin Luo,Quanchao Zhang,Zhiwei Yang,Yizao Wan 한국섬유공학회 2023 Fibers and polymers Vol.24 No.11

Natural blood vessels possess a multi-layered structure. Herein, we present a bio-inspired double-layered integrated tubular graft with a compact inner layer and a porous outer layer. The inner layer consists of nanofibrous bacterial cellulose (BC) and microfibrous cellulose acetate (CA), which favors the adhesion and proliferation of endothelial cells (ECs). The outer layer, with hierarchical pores including nano-sized pores from pristine BC, median-sized pores (dozens of microns) created by particle leaching, and a large pore (hundreds of microns) array created by laser ablation, is appropriate for the penetration of vascular smooth muscle cells (SMCs). The in-situ biosynthesis was employed to "suture" the two layers, aiming to achieve an integrated tubular structure. The morphology, mechanical properties, and cell behavior of the tubular grafts were characterized. The in vitro study demonstrates that the unique outer layer possesses enhanced SMC penetration compared to the counterpart without hierarchical pores. Overall, the findings of this study demonstrate the potential of such a biomimetic integrated vascular graft for vascular replacement and regeneration.

Characterization of biomedical hydroxyapatite/magnesium composites prepared by powder metallurgy assisted with microwave sintering

Guangyao Xiong,Yanjiao Nie,Dehui Ji,Jing Li,Chunzhi Li,Wei Li,Yong Zhu,Honglin Luo,Yizao Wan 한국물리학회 2016 Current Applied Physics Vol.16 No.8

Microwave assisted sintering has attracted much attention due to the greatly reduced sintering time. In this work, for the first time, hydroxyapatite (HAp)-reinforced magnesium (Mg) composites were prepared by the microwave assisted sintering technique. The as-prepared HAp/Mg composites were characterized by mechanical and electrochemical tests, XRD analysis, and preliminary biological evaluation. Optical microscopy observation confirms the homogeneously distributed HAp particles in the Mg matrix. It is shown that the relative density of HAp/Mg composite with 10 wt% HAp can reach 96.5% after only 10 min microwave assisted sintering. Furthermore, the mechanical properties of HAp/Mg composites are significantly higher than those of pure Mg. The corrosion resistance of HAp/Mg composites evaluated by immersion and electrochemical measurements in simulated body fluid (SBF) at 37 C reveals significant improvement over pure Mg. It is demonstrated that the mechanical properties, corrosion resistance, and biological behavior can be properly controlled by adjusting HAp content.

Effect of Graphene Oxide Incorporation into Electrospun Cellulose Acetate Scaffolds on Breast Cancer Cell Culture

Yizao Wan,Zhonghong Lin,Deqiang Gan,Teng Cui,Meirong Wan,Fanglian Yao,Quanchao Zhang,Honglin Luo 한국섬유공학회 2019 Fibers and polymers Vol.20 No.8

Graphene-based nanomaterials have been used as biomaterials to enhance cell adhesion, growth, anddifferentiation. However, the effect of graphene materials on cancer cell behavior has not been thoroughly investigated. Herein, we have incorporated graphene oxide (GO) into cellulose acetate (CA) to develop nanofibrous scaffolds for in vitrocancer cell culture, which is a crucial step for drug screening and cancer research. The GO/CA scaffolds were seeded withbreast cancer cells and cell viability, proliferation, adhesion, infiltration, and morphology were assessed. Mechanicalcharacterization demonstrated that the mechanical properties of GO/CA scaffolds were significantly better than bare CAscaffold and improved with increasing GO content. More importantly, the in vitro cell studies showed that the cancer cells onGO/CA scaffolds had significantly higher viability and better cell adhesion and growth than bare CA. Our results confirm animportant role of GO in improving mechanical properties and cancer cell performance on GO/CA scaffolds. These resultssuggest the potential of the GO/CA scaffolds as a promising candidate for in vitro cancer models.