Promoter Methylation Status of DNA Repair Gene (hMLH1) in Gastric Carcinoma Patients of the Kashmir Valley

Wani, Majid,Afroze, Dil,Makhdoomi, Muzamil,Hamid, Iqra,Wani, Bilal,Bhat, Gulzar,Wani, Rauf,Wani, Khursheed Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Cancer is a multi-factorial disease and variation in genetic susceptibility, due to inherited differences in the capacity to repair mismatches in the genome, is an important factor in the development of gastric cancer (GC), for example. Epigenetic changes, including aberrant methylation of 5/CpG islands in the promoter regions of mismatch repair (MMR) genes like hMLH1, have been implicated in the development of various types of GC. In the present study we evaluated the role of hMLH1 promoter hypermethylation in Kashmiri GC patients and controls, and assessed correlations with various dietary and lifestyle factors. The study included 70 GC patients (56 males and 14 females; age ($mean{\pm}S.D$) $50{\pm}11.4$ years). Distinction between methylated and unmethylated was achieved with MS-PCR and DNA band patterns. The Chi-square test was applied to assess the risk due to promoter hypermethylation. We found a strikingly high frequency of promoter hypermethylation in GC cases than in normal samples (72.9% (51/70) in GC cases vs 20% (14/70) in normal samples (p=0.0001).We also observed a statistically significant association between methylated hMLH1 gene promoter and smoking, consumption of sundried vegetables and hot salted tea with the risk of GC. This study revealed that hMLH1 hypermethylation is strongly associated with GC and suggested roles for epigenetic changes in stomach cancer causation in the Kashmir valley.

Isoform-specific regulation of Akt by PDGF-induced reactive oxygen species

Wani, R.,Qian, J.,Yin, L.,Bechtold, E.,King, S.B.,Poole, L.B.,Paek, E.,Tsang, A.W.,Furdui, C.M. National Academy of Sciences 2011 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.108 No.26

Configuration assessment of MR dampers for structural control using performance-based passive control strategies

Wani, Zubair R.,Tantray, Manzoor A.,Iqbal, Javed,Farsangi, Ehsan Noroozinejad Techno-Press 2021 Structural monitoring and maintenance Vol.8 No.4

The use of structural control devices to minimize structural response to seismic/dynamic excitations has attracted increased attention in recent years. The use of magnetorheological (MR) dampers as a control device have captured the attention of researchers in this field due to its flexibility, adaptability, easy control, and low power requirement compared to other control devices. However, little attention has been paid to the effect of configuration and number of dampers installed in a structure on responses reduction. This study assesses the control of a five-story structure using one and two MR dampers at different stories to determine the optimal damper positions and configurations based on performance indices. This paper also addresses the fail-safe current value to be applied to the MR damper at each floor in the event of feedback or control failure. The model is mathematically simulated in SIMULINK/MATLAB environment. Linear control strategies for current at 0 A, 0.5 A, 1 A, 1.5 A, 2 A, and 2.5 A are implemented for MR dampers, and the response of the structure to these control strategies for different configurations of dampers is compared with the uncontrolled structure. Based on the performance indices, it was concluded that the dampers should be positioned starting from the ground floor, then the 2^nd floor followed by 1^st and rest of the floors sequentially. The failsafe value of current for MR dampers located in lower floors (G+1) should be kept at a higher value compared to dampers at top floors for effective passive control of multi-story structures.

GASEOUS EMISSIONS AND PARTICLE MICROSTRUCTURE CHARACTERISTICS OF PODE/DIESEL BLEND FUEL

Wanying Gao,Junheng Liu,Ping Sun,Chen Yang,Jia Fang 한국자동차공학회 2019 International journal of automotive technology Vol.20 No.3

Polyoxymethylene dimethyl ethers (PODE) has good application prospects as an alternative to diesel fuel with a great potential to achieve clean combustion on diesel engines. In current study, the effects of PODE/diesel blends with PODE volume fraction at 10 %, 20 % and 30 % on performance and emission characteristics are investigated on a common-rail diesel engine under different engine loads. The particle size distribution and microstructure characteristics of the combustion particles are also analized. Results show that the PODE/diesel blends have shorter ignition delay, higher in-cylinder maximum burst pressure and advanced heat release. The brake thermal efficiency increases slightly with the increment of PODE blending ratio. There is a simultaneous improvement in CO, HC and soot emissions when fueling blend fuels. A more obvious shifting tendency toward the direction of small particle size can be found in number concentration, mass density and mass cumulative distribution of particles with increment of PODE. The microstructure of the blend fuels combustion particles is mainly in the form of agglomerates. Compared with diesel fuel, combustion particles of blend fuels have bigger layer spacing and microcrystalline curvature as well as smaller crystallite size, also the box dimension of the particles and the degree of agglomeration increase.

Joint Hierarchical Semantic Clipping and Sentence Extraction for Document Summarization

Wanying Yan,Junjun Guo 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.4

Extractive document summarization aims to select a few sentences while preserving its main information on a given document, but the current extractive methods do not consider the sentenceinformation repeat problem especially for news document summarization. In view of the importance and redundancy of news text information,in this paper, we propose a neural extractive summarization approach with joint sentence semantic clipping and selection, which can effectively solve the problem of news text summary sentence repetition. Specifically, a hierarchical selective encoding network is constructed for both sentencelevel and documentlevel document representations, and data containing important information is extracted on news text; a sentence extractor strategy is then adopted for joint scoring and redundant information clipping. This way, our model strikes a balance between important information extraction and redundant information filtering. Experimentalresults on both CNN/Daily Mail dataset and Court Public Opinion News dataset we built are presented to show the effectiveness of our proposed approach in terms of ROUGE metrics, especially for redundant information filtering.

EXTENSION OF GRACE'S THEOREM TO BI-COMPLEX POLYNOMIALS

Wani, Zahid Manzoor,Shah, Wali Mohammad The Kangwon-Kyungki Mathematical Society 2022 한국수학논문집 Vol.30 No.2

In this paper, we prove some results concerning the zeros of Bi-complex polynomials. These results as special cases include Grace's theorem and related results.

Exploring Resident’s Daily Activity-Travel Behavior: Activity Pattern, Duration and Competition

Wanying Li,Hongzhi Guan,Yan Han,Haiyan Zhu,Pengfei Zhao 대한토목학회 2021 KSCE Journal of Civil Engineering Vol.25 No.8

This paper aims to deeply analyze the regularity of residents' daily activity-travel behaviors to help traffic management departments predict travel demand and evaluate transportation policies. Taking time use data in American as empirical analysis, the influencing factors such as residents’ activity pattern, duration and competition were discussed and analyzed based on the competing risk model. Results can be concluded that: a) people feel happy and meaningful during most of the activities, while they feel sad or pain in relatively rare occasions; b) there is no significant difference between young people and the elderly as for the total number of activities during one day; c) estimation results show that personal, household and activity characteristics have significant influence on activity duration and pattern, and the pattern of last activity (N) has a great influence on the occurrence of next activity (N+1); d) the competition between residents' daily activities is confirmed based on the competing risk model, and travel is closely related to various activities and can be seen as the derived demand of various activities. In all combinations of activity-travel chain schedule, personal - household (30.0%), household - recreation (25.9%), work - travel (45.2%), purchase - travel (33.7%), recreation - travel (22.5%), volunteer - travel (55.3%) and travel - purchase (28.4%) have the highest proportion respectively.

Investigation of the effect of Electrocoagulation operational parameters on the leachability of arsenic in sludge

( Wani Lukak ),( Yasumasa Tojo ) 한국폐기물자원순환학회(구 한국폐기물학회) 2018 한국폐기물자원순환학회 심포지움 Vol.2018 No.1

Mine drainage from gold mines abundant in sulphide ores is characterized by elevated arsenic (As) concentrations and other hazardous heavy metals such as Pb, Cd, Cu and Zn. Treatment of mine drainage generates tons of hazardous sludge enriched in these heavy metals. Disposal of wastewater treatment sludge requires specific attention due to the hazardous nature of the sludge. Electrocoagulation (EC) is increasingly being used for removing Arsenic (As), replacing conventional chemical coagulation due to its higher removal efficiency and lower costs. The process takes place in an electrochemical cell operated with Iron electrodes. Upon passage of current, dissolution of iron electrodes generates positively charged iron oxide/hydroxide complexes (Fe(H₂O)₆³⁺, Fe(H₂O)(OH)²⁺, Fe₂(H₂O)₆(OH)₄⁴⁺ and goethite FeO(OH)) with large adsorption surface areas. EC produces less sludge that is more stable, containing less bound water due to larger floe sizes and is easier to filter, however, the sludge may still be considered hazardous. This raises several questions pertaining to the effect of operational parameters such as initial pH, Initial As concentration on the stability of sludge. This study aims to investigate the effect of EC operational parameters on the leachability of As from sludge generated. In doing so, this would assist in optimizing the performance of the treatment that would yield the most possible benign waste. Synthetic wastewater is prepared using concentrations of anionic and cationic constituents typically found in gold mine drainage. Laboratory scale EC experiments were conducted. At first, influence of initial concentration of arsenic on the leachability of As from the sludge generated was confirmed by conducting EC tests by changing the concentration (i.e. 10 and 100mg/l). Then, EC test by changing pH was conducted. In each test, pH was kept constant at 3, 5, 7, and 9, respectively. The aim of these tests was to investigate differences in leaching due to the phase of iron oxide/hydroxide generated at the specified pH. Leaching tests were conducted on the sludge obtained from each run. Residues from the leaching tests were acid digested and analyzed. Arsenic removal efficiency was very high for both initial As concentrations (99.5% for 10, and 95.0% for 100 mg/L).The amount of Iron oxide/hydroxide generated was the limiting factor. Test condition of lower initial concentration was able to remove As at a higher efficiency due to less competition for surfaces on the iron oxide/hydroxide phases generated. Differences were also observed in the amounts of As leached. Sludge obtained from an initial 10 mg.As/L sample leached a smaller amount of As compared to 100 mg.As/L indicating the importance of Fe/As molar ratio. For the runs conducted at constant pH, higher removal efficiency was observed in the order of pH=7, 5, 9, and 3. Removal efficiency was very high for all runs (above 99%). Similar results were identified when each sludge was subjected to leaching tests. The order of the fraction (by mass) remained in the sludge after the leaching test was pH= 7 > 5 > 9 > 3. This indicates that the iron oxide/hydroxide phases formed in situ at varying pH are bound to As at varying levels. To ensure the generation of the most benign sludge possible, it is imperative to understand the interaction between operating conditions and sludge generated. Consequently, this research aims to farther investigate other operational parameters such as electrode gap and current applied. Additionally, a characterization of the sludge generated by SEM should also be conducted to elucidate on the phases formed.

Retraction: Status of Exotic Flora in Kashmir Valley

Wani, Nasir Rashid,Mushtaq, Shah Murtaza Institute of Forest Science 2022 Journal of Forest Science Vol.38 No.1

Sinapic Acid(SA) attnuated the DSS-induced inestinal fibrosis in C57/6BL mice by modulated the NLRP3 inflammasome activation and autophagy pathway

Wanying Li,Yanmin Su,Zixian Wang,Zhewen Dong,Haokun Chen,Shumei Zhong,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To observe the effect of SA on DSS-induced intestinal fibrosis in mice. The mice were randomly divided into normal, DSS and SA treated DSS mice group. Changes in body weight(BW), blood stool and diarrhea were observed. The serum levels of IL-1β, IL-6, IL-17A, IL-18 and TNF-α were determined by ELISA kits. The protein levels of NLRP3, caspase-1, ASC, IL-1 β, α-SMA, CollagenI, Beclin1, LC3II/I and p62 were detected by Western Blotting assay. Compared with the normal group, SA significantly inhibited the loss of BW and DAI score in colitis mice (P<0.05). H&E and Masson staining assay suggested the SA reduced epithelial cell damage, crypt structure loss, inflammatory cell infiltration, and the fibrogenesis in colon of colitis mice. The serum levels of IL-1β, IL-6, IL-17A, IL-18 and TNF-α were decreased by SA(P<0.05). SA was able to reduce the protein levels of NLRP3, Caspase-1, ASC, IL-1β, α-SMA, Collagen-I, p62, and increased the expressions of Beclin1 and LC3II/I in colitis mice. The study demonstrated that SA can modulate the activity of NLRP3 inflammasome and autophagy pathway to improve DSS induced intaestinal fibrosis in mice.