Prognostic Value of MGMT Promoter Methylation and TP53 Mutation in Glioblastomas Depends on IDH1 Mutation

Wang, Kai,Wang, Yin-Yan,Ma, Jun,Wang, Jiang-Fei,Li, Shao-Wu,Jiang, Tao,Dai, Jian-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Several molecular markers have been proposed as predictors of outcome in patients with glioblastomas. We investigated the prognostic significance of $O^6$-methylguanine-DNA methyltransferase (MGMT) promoter methylation and TP53 mutation status dependent on isocitrate dehydrogenase 1 (IDH1) mutation in glioblastoma patients. A cohort of 78 patients with histologically confirmed glioblastomas treated with radiation therapy and chemotherapy were reviewed retrospectively. We evaluated the prognostic value of MGMT promoter methylation and TP53 mutation status with regard to progression-free survival (PFS) and overall survival (OS). It was revealed that mutations in IDH1, promoter methylation of MGMT, TP53 mutation, age, Karnofsky performance status (KFS), and extension of resection were independent prognostic factors. In patients with an IDH1 mutation, those with an MGMT methylation were associated with longer PFS (p=0.016) and OS (p=0.013). Nevertheless, the presence of TP53 mutation could stratify the PFS and OS of patients with IDH1 wild type (p=0.003 and 0.029 respectively, log-rank). The MGMT promoter methylation and TP53 mutation were associated with a favorable outcome of patients with and without mutant IDH1, respectively. The results indicate that glioblastomas with MGMT methylation or TP53 mutations have improved survival that may be influenced by IDH1 mutation status.

Study on Springback Properties of Different Orthodontic Archwires in Archwire Bending Process

Jiang Jin-gang,Wang Zhao,Zhang Yong-de,Jiang Ji-xiong,Niu Suo-liang,Liu Yi 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.12

The archwire bending is one of processes the most frequently used in the orthodontic treatment. Furthermore, the springback of sheet metal, which is defined as elastic recovery of the part during unloading, should be taken into consideration so as to produce formed archwire within acceptable tolerance limits. In this paper, the springback angle of different alloy archwires (including NiTi alloy wire, Beta-Ti alloy wires, Chinese stainless steel wires, and Australian stainless steel wires) were performed by the theoretical calculation based on large deformation theory and the finite element analysis. A series of numerical simulations has been conducted for the bending test, which physically simulates the actual bending of alloy archwire with a robotic apparatus. The finite element analysis of springback is shown to be very consistent with the theoretical calculation results.

Image Edge Detection Based on Anti-Symmetrical Biorthogonal Wavelet Filter Banks with the Same Even Length

Xiaoyan Wang,Baochen Jiang,Chengyou Wang,Zhiqiang Yang,Xiaoyan Wang 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

With the development of the theory of wavelet transform, biorthogonal wavelet filter banks with linear phase and compact support characteristics are widely used in signal and image processing. We study the multiscale edge detection using anti-symmetrical biorthogonal wavelet filter banks with the same even length based on the analysis of the properties of this wavelet filter banks. The steps of multiscale edge detection based on anti-symmetrical biorthogonal wavelet are introduced in detail. Experimental results on test images show that compared with the anti-symmetrical biorthogonal wavelet, the proposed anti-symmetrical biorthogonal wavelet filter banks with the same even length have better performance in terms of image edge detection. Therefore, the anti-symmetrical biorthogonal wavelet filter banks with the same even length are more suitable for image edge detection.

Regulation of Antiarrhythmic Drug Propafenone Effects on the C-type KV1.4 Potassium Channel by PHo and K+

Zhiquan Wang,Jianjun Li,Xuejun Jiang,Xuejun Jiang,Neng Wang,Shimin Wang 대한의학회 2009 Journal of Korean medical science Vol.24 No.1

The effects of the antiarrhythmic drug propafenone at c-type kv1.4 channels in Xenopus laevis oocytes were studied with the two-electrode voltage-clamp techinique. Defolliculated oocytes (stage V-VI) were injected with transcribed cRNAs of ferret Kv1.4△N channels. During recording, oocytes were continuously perfused with control solution or propafenone. Propafenone decreased the currents during voltage steps. The block was voltage-, use-, and concentration- dependent manners. The block was increased with positive going potentials. The voltage dependence of block could be fitted with the sum of monoexponential and a linear function. Propafenone accelerated the inactivate of current during the voltage step. The concentration of half-maximal block (IC50) was 121 μM/L. With high, normal, and low extracellular potassium concentrations, the changes of IC50 value had no significant statistical differences. The block of propafenone was PH- dependent in high-, normal- and low- extracellular potassium concentrations. Acidification of the extracellular solution to PH 6.0 increased the IC50 values to 463 μM/L, alkalization to PH 8.0 reduced it to 58 μM/L. The results suggest that propafenone blocks the kv1.4 N channel in the open state and give some hints for an intracellular site of action.

Evaluation of Renal Pathophysiological Processes Induced by an Iodinated Contrast Agent in a Diabetic Rabbit Model Using Intravoxel Incoherent Motion and Blood Oxygenation Level-Dependent Magnetic Resonance Imaging

Yongfang Wang,Xin Zhang,Bin Wang,Yang Xie,Yi Wang,Xuan Jiang,Rongjia Wang,Ke Ren 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.5

Objective: To examine the potential of intravoxel incoherent motion (IVIM) and blood oxygen level-dependent (BOLD) magnetic resonance imaging for detecting renal changes after iodinated contrast-induced acute kidney injury (CI-AKI) development in a diabetic rabbit model. Materials and Methods: Sixty-two rabbits were randomized into 2 groups: diabetic rabbits with the contrast agent (DCA) and healthy rabbits with the contrast agent (NCA). In each group, 6 rabbits underwent IVIM and BOLD imaging at 1 hour, 1 day, 2 days, 3 days, and 4 days after an iohexol injection while 5 rabbits were selected to undergo blood and histological examinations at these specific time points. Iohexol was administrated at a dose of 2.5 g I/kg of body weight. Further, the apparent transverse relaxation rate (R2*), average pure molecular diffusion coefficient (D), pseudo-diffusion coefficient (D*), and perfusion fraction (f) were calculated. Results: The D and f values of the renal cortex (CO) and outer medulla (OM) were significantly decreased compared to baseline values in the 2 groups 1 day after the iohexol injection (p < 0.05). A marked reduction in the D* values for both the CO and OM was also observed after 1 hour in each group (p < 0.05). In the OM, a persistent elevation of the R2* was detected for 4 days in the DCA group (p < 0.05). Histopathological changes were prominent, and the pathological features of CI-AKI aggravated in the DCA group until day 4. The D, f, and R2* values significantly correlated with the histological damage scores, hypoxiainducible transcription factor-1α expression scores, and serum creatinine levels. Conclusion: A combination of IVIM and BOLD imaging may serve as a noninvasive method for detecting and monitoring CIAKI in the early stages in the diabetic kidney.

A small hairpin RNA targeting myeloid cell leukemia-1 enhances apoptosis in host macrophages infected with Mycobacterium tuberculosis

Fei-yu Wang,Yu-qing Zhang,Xin-min Wang,Chan Wang,Xiao-fang Wang,Jiang-dong Wu,Fang Wu,Wan-jiang Zhang,Le Zhang 한국미생물학회 2016 The journal of microbiology Vol.54 No.4

Myeloid cell leukemia-1 (Mcl-1) plays an important role in various cell survival pathways. Some studies indicated that the expression of Mcl-1 was upregulated in host cells during infection with the virulent Mycobacterium tuberculosis strain, H37Rv. The present study was designed to investigate the effect of inhibiting Mcl-1 expression both in vivo and in vitro on apoptosis of host macrophages infected with M. tuberculosis using a small hairpin (sh)RNA. Mcl-1 expression was detected by the real time-polymerase chain reaction, western blotting, and immunohistochemistry. Flow cytometry and transmission electron microscopy were used to measure host macrophage apoptosis. We found elevated Mcl-1 levels in host macrophages infected with M. tuberculosis H37Rv. The expression of Mcl-1 was downregulated efficiently in H37Rv-infected host macrophages using shRNA. Knockdown of Mcl-1 enhanced the extent of apoptosis in H37Rv-infected host macrophages significantly. The increased apoptosis correlated with a decrease in M. tuberculosis colony forming units recovered from H37Rv-infected cells that were treated with Mcl-1-shRNA. Reducing Mcl-1 accumulation by shRNA also reduced accumulation of the anti-apoptotic gene, Bcl-2, and increased expression of the pro-apoptotic gene, Bax, in H37Rv-infected host macrophages. Our results showed that specific knockdown of Mcl-1 expression increased apoptosis of host macrophages significantly and decreased the intracellular survival of a virulent strain of M. tuberculosis. These data indicate that interference with Mcl-1 expression may provide a new avenue for tuberculosis therapy.

Insect-specific microRNA involved in the development of the silkworm Bombyx mori

Yong Zhang,Xue Zhou,Xie Ge,Jiang-Hao Jiang,Mu-Wang Li,Shi-Hai Jia,Xiao-Nan Yang,Yun-Chao Kan,Xue-Xia Miao,Guo-Ping Zhao,Fei Li,Yong-Ping Huang 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

MicroRNAs (miRNAs) are endogenous non-coding genes that participate in post-transcription regulation by either degrading mRNA or blocking its translation. It is considered to be very important in regulating insect development and metamorphosis. Insects are the largest group of animals and are extremely valuable in biological and agriculture research. Insects are also important pests to human health and agriculture, and efforts are necessary protect both humans and plants from disease and damage. Despite their importance, insects lag behind mammals, nematodes, and plants in miRNA research. At present, only 279 insect miRNAs have been identified from Drosophila melanogaster, Anopheles gambiae, Apis mellifera, Bombyx mori, and D. pseudoobscura in miRBase, and most of these miRNAs were computationally predicted without experimental validation. Functional analysis of insect miRNAs has only been conducted in D. melanogaster.

A Method of Coupling Expected Patch Log Likelihood and Guided Filtering for Image De-noising

Wang, Shunfeng,Xie, Jiacen,Zheng, Yuhui,Wang, Jin,Jiang, Tao Korea Information Processing Society 2018 Journal of information processing systems Vol.14 No.2

With the advent of the information society, image restoration technology has aroused considerable interest. Guided image filtering is more effective in suppressing noise in homogeneous regions, but its edge-preserving property is poor. As such, the critical part of guided filtering lies in the selection of the guided image. The result of the Expected Patch Log Likelihood (EPLL) method maintains a good structure, but it is easy to produce the ladder effect in homogeneous areas. According to the complementarity of EPLL with guided filtering, we propose a method of coupling EPLL and guided filtering for image de-noising. The EPLL model is adopted to construct the guided image for the guided filtering, which can provide better structural information for the guided filtering. Meanwhile, with the secondary smoothing of guided image filtering in image homogenization areas, we can improve the noise suppression effect in those areas while reducing the ladder effect brought about by the EPLL. The experimental results show that it not only retains the excellent performance of EPLL, but also produces better visual effects and a higher peak signal-to-noise ratio by adopting the proposed method.

3D Modeling Of GNSS Vector Tracking Receiver in Urban Area

Wang Jiang-An,Cai Gang,Lu Pan,Gao Tao 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.7

3D maps may be treated as a new data source for urban navigation and used to improve positioning accuracy in urban canyons However, 3D digital maps are not available in most cities. This paper proposed a new approach to build a 3D model by using a GNSS VT receiver. The system architecture was described first, and then the method of 3D modeling by analyzing received data was presented. The premise for building the 3D model is signal classification. Analysis of data collected by the VT receiver was described in detail. Using a GNSS signal recorded in a dense urban environment showed the effectiveness of the proposed approach.

Theoretical Explanation of the Peak Splitting of Tobacco-Specific N-Nitrosamines in HPLC

Jiang, Juxing,Li, Liangchun,Wang, Mingfeng,Xia, Jianjun,Wang, Wenyuan,Xie, Xiaoguang Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.5

During the analyzing processes of the compounds, some researchers are puzzled by the analytical signals for some TSNAs (with or without splitting peaks at various pHs and temperatures). In this work, a detailed theoretical study of structural and thermal properties of the $E/Z$ isomers of TSNAs and the corresponding protonated structures was performed using DFT methods. The calculations showed that the $E$ isomers are almost stable than $Z$ isomers, while the $Z$ isomers would be more stable when in protonation. The calculated results predicted the possibility of separation of their $E$ and $Z$ isomer forms and also showed that protonation affects the dipole moment of molecules significantly (0.1-0.5 to 0.7-2.1 Debye). The calculations agreed well with the experiments that the split-up of the HPLC signal for TSNAs into two peaks are very sensitive to the pH and temperature of the mobile-phase.