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Hai Thi Hong Truong,Ki-Taek Kim,Su Kim,Hyun-Ran Kim,Myoung-Cheol Cho,Jong-Gyu Woo 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.4
Pungency in fruits of pepper (Capsicum spp.) is due to the accumulation of capsaicinoids. Pun1 is an important gene responsible for pungency. To provide information for marker-assisted selection (MAS) of pungency, we developed and evaluated five codominant markers based on sequence of the Pun1 gene. The usefulness of these gene-based markers for pungency in MAS was tested using 85 pungent and non-pungent lines and cultivars. Our results demonstrated that these markers can discriminate the pungent and non-pungent genotypes. Our results show that the five gene-based markers could be useful as a marker-assisted selection tool.
Hai Thi Hong Truong,김기택,김수,조명철,김현란,우종규 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.1
The Bs2 gene of pepper (Capsicum spp.) confers resistance to the most commonly detected races of Xanthomonas campestris pv vesicatora (Xcv). Using information from a published database, we developed gene-based markers for selection of Bs2 in pepper. High polymorphism was detected in exon 3, intron 2 and 3’-UTR of the Bs2 gene. The three regions were sequenced and numerous SNPs and In/Dels were identified between resistant and susceptible inbred lines. Seven SNPs were selected to develop SNP markers using the tetra-primer amplification refractory mutation system-PCR methodology (Tetra-primer ARMS-PCR). One SNP (G/C) was used to develop a molecular marker that can perfectly identify the presence of the bacterial spot resistance. The gene-based markers were further validated to test association with resistance in 80 lines and cultivars. The markers successfully identified a bell pepper commercial cultivar 62-2 that harbors the Bs2 gene. The successful development of the markers in this study provides a useful tool for MAS to screen the Bs2 gene in pepper.
Distribution of DArT Markers in a Genetic Linkage Map of Tomato
Truong, Hai Thi Hong,Graham, Elaine,Esch, Elisabeth,Wang, Jaw-Fen,Hanson, Peter Korean Society of Horticultural Science 2010 원예과학기술지 Vol.28 No.4
토마토풋마름병에 저항성인 $Solanum$ $lycopersicum$ H7996와 극도감수성인 $S.$ $pimpinellifolium$ WVa700 간의 교배를 통해 획득한 재조합순계계통 $F_9$ 세대의 188개체를 이용하여 유전자연관지도를 작성하였다. 유전자지도는 DarT 260종, AFLP 74종, RFLP 4종, SNP 1종 및 SSR 22종 등 총 361종의 마커로 구성되었다. 작성된 유전자지도는 총 13개의 연관군(LG)에 2042.7cM을 포함하였으며 마커간의 평균지도거리는 5.7cM이고 이중 DArT마커는 평균 7.9cM당 1개가 분포하였다. SSR 마커의 분포를 기초로 작성된 11개 연관군들은 토마토 염색체의5번과 12번을 제외한 10개 염색체에 해당하였다. DArT 마커는 다른 마커들처럼 토마토 유전체 상에 고르게 분포하였으며, 인접 마커와의 상호분석(${\leq}$ 0.5cM) 결과 클러스터링 빈도가 13.5%인 AFLP 마커보다 3배 정도 높은 38.8%의 빈도로 최고치를 나타내었다. 본 연구를 통해 토마토에서 최초로 DarT 마커를 이용한 유전자연관지도를 작성하였다. A genetic linkage map was constructed using 188 $F_9$ RILs derived from a cross between $Solanum$ $lycopersicum$ H7996 (resistant to bacterial wilt) and $S.$ $pimpinellifolium$ WVa700 (highly susceptible to bacterial wilt). The map consisted of 361 markers including 260 DArTs, 74 AFLPs, 4 RFLPs, 1 SNP, and 22 SSRs. The resulting linkage map was comprised of 13 linkage groups covering 2042.7 cM. The genetic linkage map had an average map distance between markers of 5.7 cM, with an average DArT marker density of 1/7.9 cM. Based on the distribution of anchor SSR markers, 11 linkage groups were assigned to 10 chromosomes of tomato except chromosomes 5 and 12. The DArT markers were distributed across the genome in a similar way as other markers and showed the highest frequency of clustering (38.8%) at ${\leq}$ 0.5 cM intervals between adjacent markers, which is 3 times higher than AFLPs (13.5%). The present study is the first utilization of DArT markers in tomato linkage map construction.
Distribution of DArT Markers in a Genetic Linkage Map of Tomato
Hai Thi Hong Truong,Elaine Graham,Elisabeth Esch,Jaw-Fen Wang,Peter Hanson 한국원예학회 2010 원예과학기술지 Vol.28 No.4
토마토풋마름병에 저항성인 Solanum lycopersicum H7996와 극도감수성인 S. pimpinellifolium WVa700 간의 교배를 통해 획득한 재조합순계계통 F? 세대의 188개체를 이용하여 유전자연관지도를 작성하였다. 유전자지도는 DarT 260종, AFLP 74종, RFLP 4종, SNP 1종 및 SSR 22종 등 총 361종의 마커로 구성되었다. 작성된 유전자지도는 총 13개의 연관군(LG)에 2042.7cM을 포함하였으며 마커간의 평균지도거리는 5.7cM이고 이중 DArT마커는 평균 7.9cM당 1개가 분포하였다. SSR 마커의 분포를 기초로 작성된 11개 연관군들은 토마토 염색체의5번과 12번을 제외한 10개 염색체에 해당하였다. DArT 마커는 다른 마커들처럼 토마토 유전체 상에 고르게 분포하였으며, 인접 마커와의 상호분석(≤0.5cM) 결과 클러스터링 빈도가 13.5%인 AFLP 마커보다 3배 정도 높은 38.8%의 빈도로 최고치를 나타내었다. 본 연구를 통해 토마토에서 최초로 DarT 마커를 이용한 유전자연관지도를 작성하였다. A genetic linkage map was constructed using 188 F? RILs derived from a cross between Solanum lycopersicum H7996 (resistant to bacterial wilt) and S. pimpinellifolium WVa700 (highly susceptible to bacterial wilt). The map consisted of 361 markers including 260 DArTs, 74 AFLPs, 4 RFLPs, 1 SNP, and 22 SSRs. The resulting linkage map was comprised of 13 linkage groups covering 2042.7 cM. The genetic linkage map had an average map distance between markers of 5.7 cM, with an average DArT marker density of 1/7.9 cM. Based on the distribution of anchor SSR markers, 11 linkage groups were assigned to 10 chromosomes of tomato except chromosomes 5 and 12. The DArT markers were distributed across the genome in a similar way as other markers and showed the highest frequency of clustering (38.8%) at ≤ 0.5 cM intervals between adjacent markers, which is 3 times higher than AFLPs (13.5%). The present study is the first utilization of DArT markers in tomato linkage map construction.
Hai Thi Hong Truong,최학순,조명철,이혜은,김정호 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.2
Identification of the PCR markers tightly linked to genes that encode important agronomic traits is useful for markerassisted selection (MAS). The Cf-9 locus confers resistance to most races of the leaf mold fungus Cladosporium fulvum and belongs to a large gene family. We developed three gene-based markers (8F/10R, 8F/12R, and 10F/10R) from the Cf-9 gene sequence. Of the 101 tomato genotypes tested, 9 commercial F1 hybrids carried 8F/10R-, 8F/12R-, and 10F/10R-specific bands. Our results demonstrate the usefulness of these markers for MAS for C. fulvum resistance.