Identification of anti-osteoclastogenic compounds from <i>Cleistocalyx operculatus</i> flower buds and their effects on RANKL-induced osteoclastogenesis

Tran, Phuong Thao,Ngo, Thi Quynh-Mai,Lee, Suhyun,Kim, Okwha,Tran, Huynh Nguyen Khanh,Hwangbo, Cheol,Min, Byung Sun,Lee, Jeong-Hyung ELSEVIER SCIENCE B.V.; AMSTERDAM 2019 JOURNAL OF FUNCTIONAL FOODS Vol.60 No.-

<P><B>Abstract</B></P> <P> <I>Cleistocalyx operculatus</I> flower buds are used as a main ingredient in various beverages and herbal tea in tropical areas. The present study was conducted to investigate anti-osteoclastogenic effects of ethanol extract of <I>C. operculatus</I> flower buds (ECB) and to identify anti-osteoclastogenic compounds in these buds. ECB significantly inhibited RANKL-induced osteoclast differentiation and decreased RANKL-induced the activation of NFATc1. We isolated nineteen compounds from <I>C. operculatus</I> flower buds and found that eight compounds, including maslinic acid (<B>6</B>) and its two coumaroyl analogs (<B>7</B> and <B>8</B>), significantly inhibited RANKL-induced osteoclast formation. Among these, 3-O-<I>trans</I>-<I>p</I>-coumaroyl maslinic acid (<B>8</B>) showed the most potent inhibitory effect on RANKL-induced osteoclastogenesis via impairment of c-Fos and NF-κB activation, and subsequently, NFATc1 activation. These results suggested that identification of the anti-osteoclastogenic compounds from <I>C. operculatus</I> flower buds may extend our understanding of molecular mechanisms underlying biological activities of <I>C. operculatus</I> flower buds for osteoclast-related diseases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The anti-osteoclastogenic effect of <I>Cleistocalyx operculatus</I> is demonstrated. </LI> <LI> Ethanol extract of <I>C. operculatus</I> flower buds (ECB) inhibits RANKL-induced osteoclastogenesis. </LI> <LI> Eight compounds are identified as anti-osteoclastogenic compounds from ECB. </LI> <LI> Maslinic acid and its two coumaroyl analogs inhibit RANKL-induced NFATc1 activation. </LI> <LI> The mechanism explains the anti-osteoclastogenic effect of C. operculatus flower buds. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

베트남의 Facebook 소셜 네트워크를 통한 기후변화 대응 평가

Tran Thi Mai Phuong,Nguyen Khac1,이달희,박태윤,한신 대한지구과학교육학회 2019 대한지구과학교육학회지 Vol.12 No.1

전 세계에서 베트남은 기후 변화에 가장 큰 영향을 받는 국가 중 하나이다. 현재 베트남의 기후 변화에 대 한 대응 활동은 텔레비전, 신문, 라디오, 인터넷 등과 같은 미디어에 의존하고 있다. Facebook은 베트남 사 람들이 가장 많이 사용는 소셜 네트워크이다 (베트남 의 페이스북 이용자는 5800만명으로 세계 7위). 그러 나 상대적으로 그것을 기후변화 대응에 이용하고자 하 는 관심은 적은편이다. 이 논문에서는 Facebook의 Audience Insight Tool(AIT)과 온라인 사회학 조사 방법 을 활용하여 Facebook을 통한 기후 변화 대응 활동의 현재 상태 및 효율성을 평가했다. 베트남 Facebook의 기후 변화 대응 팬 페이지의 수는 15개 정도이며, 그중 가장 인기 있는 곳의 팔로워 수가 94,721명에 이른다. 이 연구에서는 베트남에서 가장 방문자가 많은 기후 변화 대응 팬 페이지 2곳을 조사하고 평가 한 결과, Facebook을 통한 기후 변화 대응은 예상 한 것만큼 큰 효과가 없었다. 또한 온라인 설문 조사를 통해 이러한 문제의 원인을 파악해 보고자 하였다. 이 결과는 앞으 로 기후 변화 대응을 할 때 Facebook과 같은 소셜 네트 워크의 강점을 부각시키면서 해결책을 찾고자 하는 기 관들에게 과학적인 밑거름이 될 것이다. Vietnam is one of the countries most affected by climate change. Therefore, communication activities on climate change in Vietnam are focused with various media such as television, newspapers, radio, internet etc. In particular, Facebook social network is a potential media but less interested and developed. In this topic, Audience Insight tool of the Facebook social network and the online sociological survey method were conducted to assess the current status and effectiveness of climate change communication activities via Facebook in Vietnam today. Vietnam ranks seventh in the world with 58 million users. However, the number of climate change communication fanpages has only about 15 fanpages with the largest number of followings is 94,721 persons. Among of the 10 most contented Facebook users in Vietnam today, there is no fanpage related to climate change. The results of research and evaluation two fanpages of climate change communication that are the most and most frequent followers in Vietnam showed that climate change communication via Facebook in Vietnam is not as effective as expected. At the same time, online survey results also pointed out the causes of the above problems. This is the scientific basis for management agencies to find the solutions to promote the strength of Facebook social network in climate change communication in the future.

Spectrum of Lysosomal Storage Disease Targeted for Newborn Screening in Vietnam

Dung, Vu Chi,Ngoc, Can Thi Bich,Khanh, Nguyen Ngoc,Mai, Do Thi Thanh,Thao, Bui Phuong,Mai, Tran Thi Chi,Ha, Nguyen Thu,Huong, Nguyen Thi Mai,Nam, Nguyen Hoang,Hai, Le Thanh,Tomatsu, Shunji,Yoo, Han-Wo Association for Research of MPS and Rare Diseases 2017 Journal of mucopolysaccharidosis and rare disease Vol.3 No.1

Cholinesterase Inhibitory Activities of Alkaloids from Corydalis Tuber

Tran Manh Hung,Phuong Thien Thuong,Nguyen Trung Nhan,Nguyen Thi Thanh Mai,Tran Le Quan,최재수,우미희,민병선,배기환 한국생약학회 2011 Natural Product Sciences Vol.17 No.2

Several isoquinoline alkaloids (1 - 18), which have basic chemical structures as protoberberine and aporphine skeletones, were evaluated for their inhibitory activities on AChE and BuChE. Among them, compounds 3, 4, 6, 8 and 12 showed the potent AchE activity with the IC50 values ranging from 10.2 ? 0.5 mM to 24.5 ? 1.6 mM, meanwhile, compound 14 - 17 exhibited strong inhibitory activity with IC50 values from 2.1 ? 0.2 to 5.5 ? 0.3 mM. Compounds 14 - 17 exhibited selective inhibition for AChE compared with BuChE. The isoquinoline alkaloid possesses aromatic methylenedioxy groups and quaternary nitrogen atoms are crucial for the anti-cholinesterase inhibitory activity.

A Codon-Optimized Nucleic Acid Hydrolyzing Single-Chain Antibody Confers Resistance to Chrysanthemums Against Chrysanthemum Stunt Viroid Infection

Tran, Dung Thi,Cho, Seungchan,Hoang, Phuong Mai,Kim, Jaedeok,Kil, Eui-Joon,Lee, Taek-Kyun,Rhee, Yong,Lee, Sukchan Springer-Verlag 2016 Plant molecular biology reporter Vol.34 No.1

<P>Chrysanthemum stunt viroid (CSVd), the smallest plant pathogen known to infect chrysanthemums, is a single-stranded circular RNA viroid that induces stunting that results in an overall height reduction of 30-50 % in mature plants. A catalytic single-chain variable antibody, 3D8 scFv, which exhibits intrinsic DNase and RNase activities, was expressed in chrysanthemums to generate transgenic plant resistance to CSVd infection. Moreover, a codon-optimized version of the 3D8 scFv gene for chrysanthemums was also transformed into plants; these codon-optimized transgenic chrysanthemums expressed twice as much 3D8 scFv and displayed 60 % more resistance to CSVd infection, compared with transgenic chrysanthemums harboring the original 3D8 scFv gene. CSVd challenge experiments with codon-optimized and original 3D8 scFv-transgenic chrysanthemums showed that CSVd in newly produced leaves of both codon-optimized and original 3D8 scFv-transgenic plants was not detected by RT-PCR. This is the first report describing the development of a CSVd-resistant chrysanthemum harboring a catalytic single-chain antibody, 3D8 scFv, which has intrinsic RNase activity.</P>

Intra-Organizational Factors Affecting Business Performance: An Empirical Study in Vietnam

Khuong Ngoc MAI,Thao Thi Thanh NGUYEN,Phuong Ngoc Duy NGUYEN,Khoa Tien TRAN 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.10

In the era of industry 4.0 with the robust digital transformation, especially under the trigger of the Covid-19 pandemic, the process of transforming businesses to achieve the desired business performance depends much on the mindset transformation of each member of the organization, beginning with the thoughts of leadership and stakeholders. This study will evaluate the relationship between leadership’s strategic reasoning perspectives on employee engagement or commitment and the company’s reputation, thereby directly or indirectly affecting organizational performance. The study examines data from 382 companies out of 500 samples in typical industries in Vietnam using the exploratory factor analysis (EFA) and partial least squares structural equation modeling (PLS-SEM) techniques. The results show that holistic thinking is closely related to employee retention and corporate reputation, thereby increasing the business outcomes of the organization, whereas there was no evidence to support analytical thinking in this study. As a consequence, transforming the business to achieve the desired business performance is heavily reliant on changing the mindset of each member of the organization, beginning with the top leaders and influencers of the business. This will assist Vietnamese leaders in gaining a comprehensive understanding of corporate governance and controlling the relationships between organizational constructs.

Study on the Characteristics of X-ray Spectra in Imaging Diagnosis Using Monte Carlo Simulations

Khanh Ai Tran,Loan Thi Hong Truong,Nhon Van Mai,Phuong Nguyen Dang,Dung Thi Thuy Vo 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.69 No.7

X-rays are widely used in diagnostic imaging such as radiography and computed tomography. An understanding of the X-ray spectrum is essential for estimating the dose absorbed by the patient and for improving image quality. In this research, X-ray emission spectra were investigated by using two different approaches: a Monte Carlo method using the MCNP5 code and a semi-empirical calculation with SpekCalc software. The results were compared with each other to validate the Monte Carlo simulation of the X-ray spectra, so that the simulation model can be applied to estimating the dose rate distribution in X-ray imaging. The obtained results show good agreement between the two approaches, but some divergence is still observed in the characteristic X-ray peaks. In addition, the influence of X-ray tube parameters such as the peak voltage, filter, anode angle and target material on of the beam quality was investigated.

Determinants of the Competitiveness of Women-Owned Small- and Medium-Sized Enterprises: An Empirical Study from Vietnam

Tien Ngoc DAO,Ha Thi Thu LE,Phuong Thi Mai CHU,Ngan Hoang PHAM,Trang Thi Dai LUONG,Dung Tri TRAN 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.12

Guided by a resource-based theory, this study is the first one that takes a quantitative approach to identify determinants of competitiveness of women-owned small and medium enterprises (SMEs) in Vietnam. The study employs time series data of Vietnamese SMEs extracted from the Vietnam Small and Medium Enterprises Survey conducted biennially from 2005 to 2015 in ten Vietnamese provinces. Firm competitiveness hereby is indicated by revenue, market share, profitability, and export volume. The research reveals a number of determining factors, of all, research and development, labor skills, business environment, technology investment are the most important factors, followed by capital and headcount. It is indicated that the determining factors have different influences on competitiveness obtained by different measurements. Therefore, it is based on specific targets and situations to make wise business decisions. The authors also make comparisons among groups of women-owned enterprises divided by their firm age, location, ownership, export, age, and educational background of business owners. The findings serve as critical empirical evidence and provide policy recommendations for improving the competitiveness of women-owned SMEs in Vietnam. The recommendations range from technology support, education and professional support for female entrepreneurs, access to capital and human resources to business environment improvement.

Synthesis, bioevaluation and docking study of 5-substitutedphenyl-1,3,4-thiadiazole-based hydroxamic acids as histone deacetylase inhibitors and antitumor agents

Nam, Nguyen-Hai,Huong, Tran Lan,Dung, Do Thi Mai,Dung, Phan Thi Phuong,Oanh, Dao Thi Kim,Park, Sang Ho,Kim, Kyungrok,Han, Byung Woo,Yun, Jieun,Kang, Jong Soon,Kim, Youngsoo,Han, Sang-Bae Informa UK Ltd. 2014 Journal of enzyme inhibition and medicinal chemist Vol.29 No.5

<P>Since the first histone deacetylase (HDAC) inhibitor (Zolinza®, widely known as suberoylanilide hydroxamic acid; SAHA) was approved by the Food and Drug Administration for the treatment of T-cell lymphoma in 2006, the search for newer HDAC inhibitors has attracted a great deal of interest of medicinal chemists worldwide. As a continuity of our ongoing research in this area, we designed and synthesized a series of 5-substitutedphenyl-1,3,4-thiadiazole-based hydroxamic acids as analogues of SAHA and evaluated their biological activities. A number of compounds in this series, for example, <I>N<SUP>1</SUP></I>-hydroxy-<I>N</I><SUP>8</SUP>-(5-(2-chlorophenyl)-1,3,4-thiadiazol-2-yl)octandiamide (<B>5b</B>), <I>N<SUP>1</SUP></I>-hydroxy-<I>N</I><SUP>8</SUP>-(5-(3-chlorophenyl-1,3,4-thiadiazol-2-yl)octandiamide (<B>5c</B>) and <I>N<SUP>1</SUP></I>-hydroxy-<I>N</I><SUP>8</SUP>-(5-(4-chlorophenyl)-1,3,4-thiadiazol-2-yl)octandiamide (<B>5d</B>), were found to possess potent anticancer cytotoxicity and HDAC inhibition effects. Compounds <B>5b</B>-<B>d</B> were generally two- to five-fold more potent in terms of cytotoxicity compared to SAHA against five cancer cell lines tested. Docking studies revealed that these hydroxamic acid displayed higher affinities than SAHA toward HDAC8.</P>

Impact of Consumer Ethnocentrism on Reasoned Action and Brand Equity: Empirical Evidence from Local Fashion Brands in Vietnam

Minh Sang VO,Mai Tran NGUYEN,Tuong Vi LE,Gia Bao NGUYEN,My Duyen HO,Thi Phuong Thao PHAM 한국유통과학회 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.5

Purpose: The is study aimed to examine the impact of consumer ethnocentrism on reasoned action and brand equity on Generation Z in Vietnam. Research design, data and methodology: A quantitative study was undertaken on 302 Generation Z members. The data for the study was collected using a Google Form-based questionnaire from December 2021 to January 2022. Descriptive statistics, Cronbach’s alpha, and structural equation modeling were among the data analysis techniques employed. The results: The results show that consumer ethnocentrism has a direct positive impact on reasoned action (subjective norms and attitude toward domestic goods) as well as an indirect positive impact on brand equity, including brand awareness, brand quality, and brand image. Major findings: According to the findings of this study, governments should continue to push propaganda and advocacy programs, call for national pride and encourage home consumers to support and use domestic goods. Domestic brands must strengthen their ties to the community and invest in community-oriented programs that promote domestic consumption. Vietnam’s domestic fashion brands need to promote the exploitation of community-oriented and nationalistic content to call on domestic consumers to support them to consume domestic brands.