Microbacterium suwonense sp. nov., Isolated from Cow Dung

Rangasamy Anandham,Tomohiko Tamura,Moriyuki Hamada,원항연,Soo-Jin Kim,Yi-Seul Kim,Ken-ichiro Suzuki,권순우 한국미생물학회 2011 The journal of microbiology Vol.49 No.5

An actinomycete strain, designated M1T8B9^T, was isolated from cow dung in Suwon, Republic of Korea. The isolate was a Gram-positive, nonmotile, and non-spore-forming bacterium. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the genus Microbacterium, with its closest neighbors being Microbacterium soli DCY17^T (98.2%) and Microbacterium esteraromaticum DSM 8609^T (98.0%). The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, and one unknown glycolipid. Strain M1T8B9^T contained the major fatty acids C_(15:0) anteiso, C_(16:0) iso, C_(17:0) anteiso, and C_(15:0) iso, and the cell-wall peptidoglycan was of type B2β. According to DNA-DNA hybridization studies, strain M1T8B9^T showed 42% and 26% relatedness with M. soli DCY17^T and M. esteraromaticum DSM 8609^T, respectively. On the basis of the data presented, strain M1T8B9^T is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium suwonense sp. nov. is proposed. The type strain is M1T8B9^T (=KACC 14058^T =NBRC 106310^T).

Diaminobutyricimonas aerilata gen. nov., sp. nov., a Novel Member of the Family Microbacteriaceae Isolated from an Air Sample in Korea

장윤희,김수진,Moriyuki Hamada,Tomohiko Tamura,안재형,원항연,Ken-ichiro Suzuki,권순우 한국미생물학회 2012 The journal of microbiology Vol.50 No.6

A novel isolate, designated 6408J-67T, was isolated from an air sample collected from Jeju Island, Republic of Korea. Its phenotypic, genotypic, and chemotaxonomic properties were compared with those of members of the family Microbacteriaceae. The Gram-positive, aerobic, motile rod formed light yellow, smooth, circular and convex colonies. Optimal growth occurred at 30°C and pH 7.0. 16S rRNA gene sequence data showed that the isolate was a novel member of the family Microbacteriaceae, with the highest sequence similarity (97.4%) to Labedella gwakjiensis KSW2-17T and less (<97%) sequence similarity with other taxa. The major cellular fatty acids (>10% of the total) were anteiso-C15:0, iso-C14:0, and iso-C16:0. The strain also contained MK-13, MK-12, and MK-14 as the major menaquinones, as well as diphosphatidylglycerol, phosphatidylglycerol, and two unknown glycolipids. Its peptidoglycan structure was B1β with 2,4-diaminobutyric acid as a diamino acid. Mycolic acids were absent. The DNA G+C content was 68.3 mol%. Based on these phenotypic and genotypic findings, strain 6408J-67T represents a novel species of a new genus within the family Microbacteriaceae, for which the name Diaminobutyricimonas aerilata gen. nov., sp. nov. is proposed. The type strain is 6408J-67T (=KACC 15518T =NBRC 108726T).

Compostimonas suwonensis gen. nov., sp. nov., isolated from spent mushroom compost

Kim, Soo-Jin,Tamura, Tomohiko,Hamada, Moriyuki,Ahn, Jae-Hyung,Weon, Hang-Yeon,Park, In-Cheol,Suzuki, Ken-ichiro,Kwon, Soon-Wo Microbiology Society 2012 International journal of systematic and evolutiona Vol.62 No.10

<P>A Gram-positive, aerobic, non-motile, short rod, designated SMC46<SUP>T</SUP>, was isolated from a spent mushroom compost sample collected in the Suwon region, South Korea. 16S rRNA gene sequence analysis revealed that strain SMC46<SUP>T</SUP> was a member of the family <I>Microbacteriaceae</I>; however, the isolate formed a branch separate from other genera within the family. Sequence similarity between strain SMC46<SUP>T</SUP> and other members of the family <I>Microbacteriaceae</I> was ≤97 %, the highest sequence similarity being with <I>Frigoribacterium faeni</I> 801<SUP>T</SUP> and <I>Frondihabitans australicus</I> E1HC-02<SUP>T</SUP> (both 97.0 %). Some chemotaxonomic properties of strain SMC46<SUP>T</SUP> were consistent with those of the family <I>Microbacteriaceae</I>: MK-11 and MK-12 as the predominant menaquinones, anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 as the major cellular fatty acids and diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid as the polar lipids. However, strain SMC46<SUP>T</SUP> contained a B-type peptidoglycan not previously found in the family <I>Microbacteriaceae</I>. The DNA G+C content was 68 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain SMC46<SUP>T</SUP> was considered to represent a novel genus and species in the family <I>Microbacteriaceae</I>, for which the name <I>Compostimonas suwonensis</I> gen. nov., sp. nov. is proposed. The type strain of the type species is SMC46<SUP>T</SUP> ( = KACC 13354<SUP>T</SUP> = NBRC 106304<SUP>T</SUP>).</P>

Leucobacter denitrificans sp. nov., Isolated from Cow Dung

원항연,Rangasamy Anandham,Tomohiko Tamura,Moriyuki Hamada,김수진,김이슬,Ken-ichiro Suzuki,권순우 한국미생물학회 2012 The journal of microbiology Vol.50 No.1

The bacterial strain M1T8B10T was isolated from cow dung in Suwon, Republic of Korea. The strain was a Gram stainpositive rod, nonmotile, and non-spore-forming. According to 16S rRNA gene sequence analysis, the strain fell within the clade of the genus Leucobacter, showing the highest sequence similarities with Leucobacter aridicollis L-9T (98.7%), Leucobacter iarius 40T (98.4%), and Leucobacter komagatae JCM 9414T (98.2%). Cell-wall peptidoglycan contained the diagnostic diamino acid 2,4-diaminobutyric acid of the genus Leucobacter, showing B-type cross-linked peptidoglycans. The major fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0. The quinone system consisted of the menaquinones MK-11 (78%) and MK-10 (22%). The polar lipid profiles contained diphosphatidylglycerol, phosphatidylglycerol, and an unidentified glycolipid. Differences in several physiological features including nitrate reduction enabled the isolate to be differentiated from all recognized Leucobacter species. Based on these phylogenetic, chemotaxonomic, and phenotypic results, the isolate represents a novel species, for which the name Leucobacter denitrificans sp. nov. is proposed. The type strain is M1T8B10T (=KACC 14055T =NBRC 106309T).

Lactobacilus nuruki sp. nov., isolated from Nuruk, a Korean fermentation starter

Heo, Jun,Saitou, Satomi,Tamura, Tomohiko,Cho, Hayoung,Kim, Ji-Seon,Joa, Jae-Ho,Kim, Jeong-Seon,Kwon, Soon-Wo,Kim, Soo-Jin Microbiology Society 2018 International journal of systematic and evolutiona Vol.68 No.10

Homoserinimonas aerilata gen. nov., sp. nov., a Novel Member of the Family Microbacteriaceae Isolated from an Air Sample in Korea

김수진,장윤희,Moriyuki Hamada,Tomohiko Tamura,안재형,원항연,Ken-ichiro Suzuki,권순우 한국미생물학회 2012 The journal of microbiology Vol.50 No.4

A bacterial strain isolated from an air sample, strain 5317J-19T, was characterized. The isolate was an aerobic, motile,Gram-positive rod. The organism was able to grow between 4 and 35°C and between pH 6 and 9. The predominant fatty acids were anteiso-C15:0 and iso-C16:0. The major respiratory menaquinones were MK-12 and MK-11, and the minor ones were MK13, MK-10, and MK-9. Genomic DNA G+C content was 66 mol%. The diagnostic diamino acid of the peptidoglycan is presumably D-Orn. The peptidoglycan is supposed to be B2β type. The 16S rRNA gene sequence analysis indicated that this isolate belongs to the family Microbacteriaceae and had the highest sequence similarities with Salinibacterium xinjiangense 0543T (97.6%), Salinibacterium amurskyense KMM 3673T (97.2%), and Leifsonia bigeumensis MSL-27T (97.2%). Phylogenetic analysis and phenotypic characteristics support the proposal of a new genus and a novel species, with the name Homoserinimonas aerilata gen. nov.,sp. nov. The type strain of Homoserinimonas aerilata is 5317J-19T (=KACC 15522T =NBRC 108729T).

<i>Lysinimonas soli</i> gen. nov., sp. nov., isolated from soil, and reclassification of <i>Leifsonia kribbensis</i> Dastager <i>et al.</i> 2009 as <i>Lysinimonas kribbensis</i> sp. nov., comb. nov.

Jang, Yun-Hee,Kim, Soo-Jin,Tamura, Tomohiko,Hamada, Moriyuki,Weon, Hang-Yeon,Suzuki, Ken-ichiro,Kwon, Soon-Wo,Kim, Wan-Gyu International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.4

<P>A Gram-stain-positive, non-motile rod, designated strain SGM3-12<SUP>T</SUP>, was isolated from paddy soil in Suwon, Republic of Korea. 16S rRNA gene sequence analysis revealed that the strain represented a novel member of the family <I>Microbacteriaceae</I>. The nearest phylogenetic neighbour was <I>Leifsonia kribbensis</I> MSL-13<SUP>T</SUP> (97.4 % 16S rRNA gene sequence similarity). Strain SGM3-12<SUP>T</SUP> and <I>Leifsonia kribbensis</I> MSL-13<SUP>T</SUP> formed a distinct cluster within the family <I>Microbacteriaceae</I>. Strain SGM3-12<SUP>T</SUP> contained MK-12(H<SUB>2</SUB>) and MK-11(H<SUB>2</SUB>) as the predominant menaquinones with moderate amounts of MK-12 and MK-11; anteiso-C<SUB>15 : 0</SUB> and iso-C<SUB>16 : 0</SUB> as the major cellular fatty acids (>10 % of total); and diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipids as the polar lipids. The peptidoglycan type of the isolate was B1δ with <SMALL>l</SMALL>-Lys as the diagnostic cell-wall diamino acid. On the basis of these results, strain SGM3-12<SUP>T</SUP> represents a novel species within a new genus, for which the name <I>Lysinimonas soli</I> gen. nov., sp. nov. is proposed (the type strain of the type species is SGM3-12<SUP>T</SUP> = KACC 13362<SUP>T</SUP> = NBRC 107106<SUP>T</SUP>). It is also proposed that <I>Leifsonia kribbensis</I> be transferred to this genus as <I>Lysinimonas kribbensis</I> comb. nov. (the type strain is MSL-13<SUP>T</SUP> = DSM 19272<SUP>T</SUP> = JCM 16015<SUP>T</SUP> = KACC 21108<SUP>T</SUP> = KCTC 19267<SUP>T</SUP>).</P>

<i>Rudaibacter terrae</i> gen. nov., sp. nov., isolated from greenhouse soil

Kim, Soo-Jin,Moon, Ji-Young,Hamada, Moriyuki,Tamura, Tomohiko,Weon, Hang-Yeon,Suzuki, Ken-ichiro,Kwon, Soon-Wo International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.11

<P>A novel Gram-stain-positive, non-motile, rod-shaped bacterium, designated 5GHs34-4<SUP>T</SUP>, was isolated from greenhouse soil in Yongin, Republic of Korea. Growth occurred in the temperature range of 10–37 °C (optimum 28–30 °C) and at pH 5.0–9.0 (optimum pH 7.0). It can tolerate up to 3 % (w/v) NaCl. The strain showed 16S rRNA gene sequence similarity levels of 95.1–97.0 % with species of the genus <I>Leifsonia</I>, 95.7–96.7 % with species of the genus <I>Herbiconiux</I>, 95.1–96.4 % with species of the genus <I>Salinibacterium</I> and 96.1 % with <I>Labedella gwakjiensis</I> and <I>Homoserinimonas aerilata</I>. The highest sequence similarities (97.0 %) were with <I>Leifsonia aquatica</I> JCM 1368<SUP>T</SUP>, <I>Leifsonia poae</I> VKM Ac-1401<SUP>T</SUP> and <I>Leifsonia psychrotolerans</I> LI1<SUP>T</SUP>. The peptidoglycan type determined for strain 5GHs34-4<SUP>T</SUP> was B2γ with <SMALL>dl</SMALL>-2,4-diaminobutyric acid at position 3. The murein was of the acetyl type. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The menaquinones detected were MK-13, MK-12 and MK-14, and the major fatty acids were summed feature 8 (C<SUB>18 : 1</SUB>ω7<I>c</I> and/or C<SUB>18 : 1</SUB>ω6<I>c</I>), anteiso-C<SUB>17 : 0</SUB> and anteiso-C<SUB>15 : 0</SUB>. The phenotypic and phylogenetic traits of strain 5GHs34-4<SUP>T</SUP> differed in some respects from those of members of the family <I>Microbacteriaceae</I>. Therefore, strain 5GHs34-4<SUP>T</SUP> is considered to represent a novel species of a new genus in the family <I>Microbacteriaceae</I>, for which the name <I>Rudaibacter terrae</I> gen. nov., sp. nov. is proposed. The type strain is 5GHs34-4<SUP>T</SUP> ( = KACC 15523<SUP>T</SUP> = NBRC 108754<SUP>T</SUP>).</P>

Angustibacter aerolatus sp. nov., isolated from air.

Kim, Soo-Jin,Jang, Yun-Hee,Hamada, Moriyuki,Tamura, Tomohiko,Ahn, Jae-Hyung,Weon, Hang-Yeon,Suzuki, Ken-ichiro,Kwon, Soon-Wo Society for General Microbiology 2013 International journal of systematic and evolutiona Vol.63 No.2

<P>A novel actinomycete strain, 7402J-48(T), was isolated from an air sample collected from Jeju island, Republic of Korea. Cells were gram-positive, aerobic, flagellated, short rods. Strain 7402J-48(T) showed highest 16S rRNA gene sequence similarity (98.6?%) with Angustibacter luteus TT07R-79(T), and had relatively low sequence similarities (below 95.1?%) with other members of the family Kineosporiaceae. The cell wall of strain 7402J-48(T) contained alanine, glutamic acid and 2,6-diaminopimelic acid, suggesting A1γ-type peptidoglycan. The menaquinones were MK-9(H(4)) and MK-8(H(4)). The acyl type of the cell-wall muramic acid was acetyl. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unknown lipid were present. The cellular fatty acid profile comprised a large amount of anteiso-C(15?:?0), moderate amounts of C(16?:?0), summed feature 3 (including C(16?:?1)ω7c and/or C(16?:?1)ω6c), C(17?:?0), iso-C(15?:?0), C(16?:?0) N alcohol and C(17?:?1)ω8c, and small amounts of other fatty acids. The DNA G+C content of strain 7402J-48(T) was 73 mol%. Based on its phenotypic and genotypic characteristics, strain 7402J-48(T) (?=?KACC 15527(T)?=?NBRC 108730(T)) is proposed as the type strain of a novel species, Angustibacter aerolatus sp. nov. An emended description of the genus Angustibacter is provided.</P>

Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China.

Xing, Ke,Liu, Wei,Zhang, Yue-Ji,Bian, Guang-Kai,Zhang, Wen-Di,Tamura, Tomohiko,Lee, Jung-Sook,Qin, Sheng,Jiang, Ji-Hong N.V. Swets en Zeitlinger 2013 Antonie van Leeuwenhoek Vol.103 No.2

<P>A novel actinomycete, designated strain KLBMP 1262(T), was isolated from a coastal plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1262(T) is a member of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1262(T) is most closely related to Amycolatopsis sulphurea DSM 46092(T) (97.96 %), Amycolatopsis ultiminotia RP-AC36(T) (97.50 %) and Amycolatopsis jejuensis N7-3(T) (97.44 %); similarity to other type strains of the genus Amycolatopsis was less than 97.0 %. The organism was determined to have chemical and morphological features consistent with its classification in the genus Amycolatopsis such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was determined to be MK-9 (H(4)). The polar lipids detected were phosphatidylmethylethanolamine, phosphatidylethanolamine, an unknown aminophospholipid, two unknown glycolipids and several unknown lipids. The major fatty acids were found to be C(16:0), iso-C(16:0) and iso-C(15:0). DNA-DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate is considered to represent a novel species, for which the name A. jiangsuensis sp. nov. is proposed. The type strain is KLBMP 1262(T) (=KCTC 19885 (T) = NBRC 108679(T)).</P>