Anti-obesity effect of Burdock Vinegar on High Fat Diet Fed Rats

Yi-Seul Kim,In-Ho Kim,In-Ju Eom,Seong-Ho Kim 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Effects of e-health literacy and oral health knowledge on oral health behavior in adults

Yi Seul Kim,Soon Ryun Lim 한국치위생학회 2022 한국치위생학회지 Vol.22 No.1

Objectives: The purpose of this study was to identify new variables that can enhance adult oral health behaviors by confirming the degree of adult e-health literacy, oral health knowledge, and oral health behaviors and examining their relevance. Methods: A self-reported questionnaire was filled out by 350 adults from June 22 to August 1, 2021. Data were analyzed using SPSS 23.0. independent t-test, one way ANOVA, the scheffé post-hoc test and the pearson correlation coefficients were reviewed, A hierarchical regression analysis was conducted. Results: Oral health behaviors according to general characteristics showed significant differences in gender, educational background, dental visit within 1 year, subjective oral health status, oral health interest, frequency of oral internet use, and reliability of internet oral health information. Also it was found that e-health literacy affects oral health behavior. Conclusions: In this study, e-health literacy, oral health knowledge, and oral health behavior were correlated and it was confirmed that e-health literacy had an effect on oral health behavior. In the future, it is necessary to develop a tool that can measure e-oral health literacy and to find a way to improve the oral health behavior of adults by using e-oral health literacy.

Co-aggregation of Enzyme and Amyloid Fibrils

Seul Gi KIM,Song Yi BAE,T. Doohun Kim 한국생물공학회 2011 한국생물공학회 학술대회 Vol.2011 No.10

Analyses of Bacterial Communities in Meju, a Korean Traditional Fermented Soybean Bricks, by Cultivation-Based and Pyrosequencing Methods

Yi-Seul Kim,김민철,권순우,Soo-Jin Kim,In-Cheol Park,가종억,원항연 한국미생물학회 2011 The journal of microbiology Vol.49 No.3

Despite the importance of meju as a raw material used to make Korean soy sauce (ganjang) and soybean paste (doenjang), little is known about the bacterial diversity of Korean meju. In this study, the bacterial communities in meju were examined using both culture-dependent and independent methods in order to evaluate the diversity of the bacterial population. Analyses of the 16S rRNA gene sequences of the bacterial strains isolated from meju samples showed that the dominant species were related to members of the genera Bacillus, Enterococcus, and Pediococcus. The community DNAs extracted from nine different meju samples were analyzed by barcoded pyrosequencing method targeting of the V1 to V3 hypervariable regions of the 16S rRNA gene. In total, 132,374 sequences, with an average read length of 468 bp, were assigned to several phyla, with Firmicutes (93.6%) representing the predominant phylum, followed by Proteobacteria (4.5%) and Bacteroidetes (0.8%). Other phyla accounted for less than 1% of the total bacterial sequences. Most of the Firmicutes were Bacillus and lactic acid bacteria, mainly represented by members of the genera Enterococcus, Lactococcus, and Leuconostoc, whose ratio varied among different samples. In conclusion,this study indicated that the bacterial communities in meju were very diverse and a complex microbial consortium containing various microorganisms got involved in meju fermentation than we expected before.

Role of the epithelial–mesenchymal transition and its effects on embryonic stem cells

Kim, Ye-Seul,Yi, Bo-Rim,Kim, Nam-Hyung,Choi, Kyung-Chul Nature Publishing Group 2014 Experimental and molecular medicine Vol.46 No.8

<P>The epithelial–mesenchymal transition (EMT) is important for embryonic development and the formation of various tissues or organs. However, EMT dysfunction in normal cells leads to diseases, such as cancer or fibrosis. During the EMT, epithelial cells are converted into more invasive and active mesenchymal cells. E-box-binding proteins, including Snail, ZEB and helix–loop–helix family members, serve as EMT-activating transcription factors. These transcription factors repress the expression of epithelial markers, for example, E-cadherin, rearrange the cytoskeleton and promote the expression of mesenchymal markers, such as vimentin, fibronectin and other EMT-activating transcription factors. Signaling pathways that induce EMT, including transforming growth factor-β, Wnt/glycogen synthase kinase-3β, Notch and receptor tyrosine kinase signaling pathways, interact with each other for the regulation of this process. Although the mechanism(s) underlying EMT in cancer or embryonic development have been identified, the mechanism(s) in embryonic stem cells (ESCs) remain unclear. In this review, we describe the underlying mechanisms of important EMT factors, indicating a precise role for EMT in ESCs, and characterize the relationship between EMT and ESCs.</P>

Rhodanobacter umsongensis sp. nov., Isolated from a Korean Ginseng Field

Yi-Seul Kim,김수진,Rangasamy Anandham,원항연,권순우 한국미생물학회 2013 The journal of microbiology Vol.51 No.2

A bacterial isolate designated GR24-2T was isolated from Korean soil used for cultivating ginseng (Panax ginseng C. A. Meyer). The strain was aerobic, Gram-negative, motile,and rod-shaped. It grew optimally at 28–30°C, pH 7.0, and in a range of 0–1% NaCl. Phylogenetically, the strain clustered with members of the genus Rhodanobacter. The strain exhibited the highest sequence similarities (>98%) with R. panaciterrae LnR5-47T (98.4%), R. soli DCY45T (98.2%), and R. ginsengisoli GR17-7T (98.0%). However, it also showed high sequence similarities (>97%) with some other Rhodanobacter and Dyella species. The strain contained Q-8 as the predominant respiratory quinone. The major fatty acids (greater than 10% of the total fatty acids) were iso-C17:1 ω9c (24.5%), iso-C16:0 (22.8%), anteiso-C15:0 (10.5%), and iso-C15:0(10.1%). Its major polar lipids were phosphatidylethanolamine,phosphatidylglycerol, diphosphatidylglycerol, and an unknown aminophospholipid. The DNA G+C content of strain GR24-2T was 65.6 mol%. The strain showed less than 70% DNA relatedness values between the closely related Rhodanobacter and Dyella species. The phylogeny, phenotype,DNA–DNA hybridization, and chemotaxonomic data generated in this study reveal that the isolate is a novel species of the genus Rhodanobacter. The name proposed for this strain is Rhodanobacter umsongensis sp. nov. (type strain GR24-2T =KACC 12917T =DSM 21300T).

Origin-related study of genetic diversity and heteroplasmy of Mongolian sheep (Ovis aries) using mitochondrial DNA

Yi Seul Kim,Khaliunaa Tseveen,Badamsuren Batsukh,Jiyeon Seong,Hong Sik Kong 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.2

Food and agricultural production sector, especially livestock production is vital for Mongolia’s economic and social development. Domestic sheep play key roles for Mongolians, providing food (meat, milk) and raw materials (wool, sheepskin), but genetic diversity, origin of sheep populations in Mongolia have not been well studied. Studies of population genetic diversity is important research field in conservation and restoration of animal breeds and genetic resources. Therefore, this study aimed to investigate genetic characteristics and estimate origin through the analysis of mitochondrial DNA control region D-loop and Cytochrome b of Mongolian indigenous sheep (Mongolian native, Orkhon and Altanbulag) and one Europe sheep (Suffolk). As a result of there were found, 220 SNPs (Single nucleotide polymorphism) in the D-loop region, 28 SNPs in the Cytochrome B region, furthermore, 77 Haplotypes. The nucleotide diversity was only found in D-loop region (n = 0.0184). Phylogenetic analysis showed that 3 (A, B, and C) of 5 haplogroups of sheep have been identified in our research. Haplogroup C was only found in Mongolian indigenous sheep. Haplogroup D and E were not observed. As a result of haplogroups, haplogroup A was dominant (n = 46 of 94 sheeps), followed by haplogroup B (n = 36) and haplogroup C (n = 12). Sequence analysis showed that T deletion, insertion and heteroplasmy in D-loop region occurred at a high rate in Mongolian indigenous sheep population (T insertion = 47, T deletion = 83). The heteroplasmy, which has never been found in Mongolian sheep, has been newly discovered in this study. As a result, the Mongolian sheep varieties, which mainly derived from Asia, were in hybridization with European sheep varieties.

Etiology and clinical characteristics of fever of unknown origin in children: a 15-year experience in a single center

Kim, Yi-Seul,Kim, Kyung-Ran,Kang, Ji-Man,Kim, Jong-Min,Kim, Yae-Jean The Korean Pediatric Society 2017 Clinical and Experimental Pediatrics (CEP) Vol.60 No.3

Purpose: Fever is one of the most common symptoms in children. In previous studies, infectious disease was the most common cause of pediatric fever of unknown origin (FUO). The aim of this study is to investigate the etiology, clinical characteristics and prognosis of pediatric FUO in 21 century with more diagnostics available and to analyze the factors for certain disease categories. Methods: Among the children under 18 years old who were hospitalized at Samsung Medical Center from January 2000 to December 2014, the patients who met the criteria including fever of ${\geq}38.0^{\circ}C$ for longer than ${\geq}14days$ and failure to reach a diagnosis after one week of investigations were included. Results: Total 100 patients were identified. Confirmed diagnosis was achieved in 57 patients (57%). Among them, infectious diseases (n=19, 19%) were most common, followed by connective tissue diseases (n=15, 15%), necrotizing lymphadenitis (n=8, 8%), and malignancies (n=7, 7%). Children with fever duration over 28 days had a trend for higher frequency of connective tissue diseases (28.3%) except undiagnosed etiology. The symptoms such as arthritis, lymph node enlargement and only fever without other symptoms were significantly related with connective tissue diseases, necrotizing lymphadenitis and undiagnosed respectively (P<0.001). Ninety-two patients have become afebrile at discharge and 1 patient died (1%). Conclusion: Almost half of our patients were left without diagnosis. Although it has been known that infectious disease was most common cause of pediatric FUO in the past, undiagnosed portion of FUO have now increased due to development of diagnostic techniques for infectious diseases.

Anti-Obesity Effect of Deodeok Vinegar on High Fat Diet Fed Rats

Yi-Seul Kim,In-Ho Kim,Seong-Ho Kim 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

Parapedobacter luteus sp. nov. and Parapedobacter composti sp. nov., isolated from cotton waste compost

Kim, Soo-Jin,Weon, Hang-Yeon,Kim, Yi-Seul,Yoo, Seung-Hee,Kim, Byung-Yong,Anandham, Rangasamy,Kwon, Soon-Wo Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.8

<P>Two aerobic, Gram-reaction-negative, non-spore-forming bacterial strains, 4M29<SUP>T</SUP> and 4M40<SUP>T</SUP>, were isolated from cotton composts. The two strains grew in the presence of 0-5 % (w/v) NaCl (optimum growth in the absence of NaCl), at pH 6.0-8.0 (optimum, pH 7.0) and at 15-45 °C (optimum, 30 °C). The strains shared 97.1 % 16S rRNA gene sequence similarity. Strains 4M29<SUP>T</SUP> and 4M40<SUP>T</SUP> showed the next highest levels of 16S rRNA gene sequence similarity to <I>Parapedobacter koreensis</I> Jip14<SUP>T</SUP> (95.6 and 94.4 %, respectively) and <I>Parapedobacter soli</I> DCY14<SUP>T</SUP> (95.2 and 93.8 %). The level of DNA-DNA relatedness between strains 4M29<SUP>T</SUP> and 4M40<SUP>T</SUP> was 38 %. The two strains contained iso-C15 : 0, summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1<I>ω</I>7<I>c</I>) and iso-C17 : 0 3-OH as major fatty acids, MK-7 as the major respiratory quinone, homospermidine as the only polyamine and phosphatidylethanolamine as the major polar lipid. The DNA G+C contents of strains 4M29<SUP>T</SUP> and 4M40<SUP>T</SUP> were 47.6 and 48.6 mol%, respectively. On the basis of phylogenetic and phenotypic data, strains 4M29<SUP>T</SUP> and 4M40<SUP>T</SUP> are considered to represent two novel species of the genus <I>Parapedobacter</I>, for which the names <I>Parapedobacter luteus</I> sp. nov. (type strain 4M29<SUP>T</SUP> =KACC 10955<SUP>T</SUP> =JCM 15977<SUP>T</SUP>) and <I>Parapedobacter composti</I> sp. nov. (type strain 4M40<SUP>T</SUP> =KACC 10972<SUP>T</SUP> =JCM 15978<SUP>T</SUP>) are proposed.</P>