Phosphate Transporter MdPHT1;7 Enhances Phosphorus Accumulation and Improves Low Phosphorus and Drought Tolerance

Sun Tingting,Zhou Beibei,Pei Tingting,Meng Hu,Zhang Junke,Ma Fengwang,Wei Qinping 한국식물학회 2021 Journal of Plant Biology Vol.64 No.5

Phosphorus is an important macronutrient for plant growth and is acquired by plants mainly as phosphate. Phosphate fertilizer is usually used to reduce inorganic phosphate (Pi) deficiency in the soil, improve the low phosphorus and drought tolerance of plants, and promote plant growth. Phosphate transporters (PHTs) play an important role in absorbing phosphorus from the soil. MdPHT1;7 was induced by Pi deficiency and drought in roots in our previous research. In this study, we cloned MdPHT1;7 and showed its heterologous expression can complement a high-affinity Pi transporter gene in the Pi uptake-defective yeast mutant BY4743. MdPHT1;7 is a phosphorus transporter located on the cell membrane. Overexpression of MdPHT1;7 in ‘Orin’ apple and ‘Micro-Tom’ tomato enhanced Pi accumulation, low Pi tolerance and drought tolerance. We hypothesized that MdPHT1;7 can enhance Pi absorption and play an important role in improving plant resistance to low phosphorus and drought stresses.

A rapid and sensitive molecularly imprinted electrochemiluminescence sensor for Azithromycin determination in biological samples

Hu, Liqin,Zhou, Tingting,Feng, Jingwen,Jin, Hua,Tao, Yun,Luo, Dan,Mei, Surong,Lee, Yong-Ill Elsevier 2018 Journal of Electroanalytical Chemistry Vol.813 No.-

<P><B>Abstract</B></P> <P>Azithromycin (AZM) is a widely used macrolide antibiotic to treat infections caused by bacteria. Considering the side-effect to human health, a rapid and selective electrochemiluminescence (ECL) sensor was fabricated on the basis of molecularly imprinted polymer (MIP) modified carbon paste electrode as a recognition element. We systematically optimized the factors influencing the processes on the extraction and determination for AZM, including the amount of MIP power and paraffin oil, extraction solution, extraction time and the pH of Ru(bpy)<SUB>3</SUB> <SUP>2+</SUP> solution. Under the optimal condition, the wide linear range was observed from 1.0 × 10<SUP>−10</SUP> to 4.0 × 10<SUP>−7</SUP> mol L<SUP>−1</SUP>, and the limit of detection (LOD) was low as 2.3 × 10<SUP>−11</SUP> mol L<SUP>−1</SUP> (S/N = 3). The spiked recoveries of AZM in urine and blood samples were from 98.4% to 113.5% with RSD changing from 3.7% to 13.2%, respectively. The results of AZM determination by this proposed sensor were in accordance with that of high-performance liquid chromatography–tandem mass spectrometry method. Thus, the developed MIP-based ECL sensor will have a great potential application in rapid and sensitive determination of trace AZM in complex biological samples.</P> <P><B>Highlights</B></P> <P> <UL> <LI> ECL sensor </LI> <LI> MIP modified CP electrode </LI> <LI> Trace AZM determined in biological samples </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Nitrogen and Oxygen Co-doped Porous Carbon Fabric for Efficient Removal of Formaldehyde

Tingting Chen,Xiaosai Hu,Tao Zhao,Yuanyu Ge 한국섬유공학회 2022 Fibers and polymers Vol.23 No.7

Formaldehyde can pose a huge threat to human health due to its’ carcinogenic effect. Carbon materials asadsorbents are widely applied to remove formaldehyde hazards. However, most carbon materials with disadvantages of thelimited number of surface functional groups and the lack of suitable pore structure suffer from low adsorption rate for theadsorptive removal of formaldehyde. To improve the adsorption capability, oxygen (O) and nitrogen (N) elements co-dopedporous viscose-based activated carbon fiber (N-O-ACF) was prepared via oxidation-hydrothermal treatment. First of all, theO atom can be introduced into ACF through chemical oxidation. Then, with the help of the O atom bridging effect, N atomscan be efficiently incorporated into ACF, and the content of the N element is as high as 7.13 wt.%. Thus, an improvedadsorption performance of N-O-ACF for formaldehyde removal was achieved with the support of the synergistic effect of Nand O elements. The adsorption capacity of N-O-ACF for removing formaldehyde increased up to 226 mg g-1, while only161.5 mg g-1 was obtained for original ACF. The excellent formaldehyde adsorption capacity indicates that N-O-ACF may bea promising adsorbent in the field of indoor air purification.

Electrochemical Degradation of Tetracycline Hydrochloride in Aqueous Medium by (B4C/C)-β-PbO2 Electrode

Tingting Xie,Hongtao Hu,Donghui Chen,Pengzhe Sun 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.7

(B4C/C)-β-PbO2 electrodes were prepared by high-pressure molding technique and characterized by X-ray diffraction, scanning electron microscopy, polarization curves, cyclic voltammetry curves, and corrosion resistance test. In this paper, the effects of initial concentration, current density, electrolyte concentration, pH, and electrode spacing on tetracycline hydrochloride (TCH) degradation were investigated. Results indicated that modified electrode had high electrochemical activities, high oxygen evolution potential, and excellent corrosion resistance. The maximum removal rate of TCH could reach 90.87% at initial concentration of 300 mg/L after 120 min electrolysis with Na2SO4 concentration of 0.2 mol/L, electrode spacing of 1 cm, current density of 30 mA/cm2, and pH 3. Pseudo-first-order kinetics equation was followed during electrocatalytic degradation of TCH with 20%(B4C/C)-β-PbO2 electrode.

Fermentation Characteristics and Lactic Acid Bacteria Succession of Total Mixed Ration Silages Formulated with Peach Pomace

Hu, Xiaodong,Hao, Wei,Wang, Huili,Ning, Tingting,Zheng, Mingli,Xu, Chuncheng Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.4

The objective of this study was to assess the use of peach pomace in total mixed ration (TMR) silages and clarify the differences in aerobic stability between TMR and TMR silages caused by lactic acid bacteria (LAB). The TMR were prepared using peach pomace, alfalfa hay or Leymus chinensis hay, maize meal, soybean meal, cotton meal, limestone, a vitamin-mineral supplement, and salt in a ratio of 6.0:34.0:44.4:7.0:5.0:2.5:1.0:0.1 on a dry matter (DM) basis. Fermentation quality, microbial composition, and the predominant LAB were examined during ensiling and aerobic deterioration. The results indicated that the TMR silages with peach pomace were well fermented, with low pH and high lactic acid concentrations. The aerobic stability of TMR silages were significantly higher than that of TMR. Compared with TMR silages with alfalfa hay, TMR silage with Leymus chinensis hay was much more prone to deterioration. Although the dominant LAB were not identical in TMR, the same dominant species, Lactobacillus buchneri and Pediococcus acidilactici, were found in both types of TMR silages after 56 d of ensiling, and they may play an important role in the aerobic stability of TMR silages.

Fermentation Characteristics and Lactic Acid Bacteria Succession of Total Mixed Ration Silages Formulated with Peach Pomace

Xiaodong Hu,Wei Hao,Huili Wang,Tingting Ning,Mingli Zheng,C.C. Xu 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.4

The objective of this study was to assess the use of peach pomace in total mixed ration (TMR) silages and clarify the differences in aerobic stability between TMR and TMR silages caused by lactic acid bacteria (LAB). The TMR were prepared using peach pomace, alfalfa hay or Leymus chinensis hay, maize meal, soybean meal, cotton meal, limestone, a vitamin-mineral supplement, and salt in a ratio of 6.0:34.0:44.4:7.0:5.0:2.5:1.0:0.1 on a dry matter (DM) basis. Fermentation quality, microbial composition, and the predominant LAB were examined during ensiling and aerobic deterioration. The results indicated that the TMR silages with peach pomace were well fermented, with low pH and high lactic acid concentrations. The aerobic stability of TMR silages were significantly higher than that of TMR. Compared with TMR silages with alfalfa hay, TMR silage with Leymus chinensis hay was much more prone to deterioration. Although the dominant LAB were not identical in TMR, the same dominant species, Lactobacillus buchneri and Pediococcus acidilactici, were found in both types of TMR silages after 56 d of ensiling, and they may play an important role in the aerobic stability of TMR silages.

Analysis of Urban Rail DC Short Circuit Fault and Vehicle-Network Protection Coordination Based on Transient Characteristics

Wang Tingting,Sun Guobin,Cao Hu,Zhang Zhexu,Chen Jinheng 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.3

During the operation of urban rail transit system, if the vehicle traction system has short-circuit fault, the vehicle protection device will act and cut off the short-circuit current. Under some extreme short circuit conditions, the protection device of power supply system will also act, and the whole power supply section will be powered off. This is because the action setting values of vehicle protection device and power supply system protection device are designed respectively, and their matching and reliability need to be analyzed. Based on this, this paper first models the urban rail transit power supply system and vehicle traction system, then compares the calculated mathematical model with the simulation model to verify the accuracy of the simulation model. Finally, the short-circuit current waveforms under different short-circuit conditions are simulated, and the action sequence and protection range of protection devices are analyzed according to the simulation results. The research results are of great significance to the characteristic analysis of short-circuit fault, the selection of vehicle protection devices and the setting of protection parameters of power supply system.

Strong adsorption of cationic dyes by cucurbit[7]uril modified magnetic carbon nanotubes: Investigation on adsorption performance, reusability, and adsorption mechanism

Yujie Hu,Changjun Zou,Tingting Xiong,Huihui Wang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

In this work, a facile one-pot method was used to synthesize the co-modified carbon nanotube adsorbent(CB/MMCNT) of cucurbit[7]uril and Fe3O4. A series of characterizations were utilized to confirm the successfulsynthesis of the adsorbent. VSM and separation experiments demonstrated that the adsorbentexhibited excellent magnetic separation performance and could achieve rapid separation within 30 s. BET results show that the adsorbent has excellent specific surface area (67.438 m2/g). Subsequently, threecationic dyes, namely Methylene blue (MB), Malachite green (MG), and Gentian violet (GV), were utilizedin batch adsorption experiments. A strong adsorption effect on cations is exerted by the adsorbentthrough hydrogen bonds, electrostatic interactions, p-p conjugation effects and hydrophobic interactions. Under the optimal conditions (pH = 6, T = 300 min, C0 = 1000 mg/L), the maximum adsorptioncapacity of CB/MMCNT to MB, MG, and GV was 851.5 mg/g, 720.2 mg/g, and 563.4 mg/g. The adsorptiondata could be well fitted by both the pseudo-second-order kinetic model (R2 > 0.99) and Langmuir isothermmodel (R2 > 0.99). Thermodynamic data indicated that the adsorption process was spontaneousand endothermic. Moreover, the cyclic reuse performance of CB/MMCNT was evaluated through 7 cyclesof recycling experiments, which demonstrated that it maintained a high dye removal rate (MB 84%, MG78%, GV 81%), indicating its excellent reusability. Additionally, CB/MMCNT still maintained its impressivecationic dye removal performance (MB 95.8%, MG 92.7%, GV 92.2%) when tested in practical water environments. Overall, CB/MMCNT is a promising, environmentally friendly, efficient, and sustainable adsorbentfor removing cationic dyes.

Backbone Cyclization of Flavin Mononucleotide-Based Fluorescent Protein Increases Fluorescence and Stability

Lin Tingting,Ge Yuanyuan,Gao Qing,Zhang Di,Chen Xiaofeng,Hu Yafang,Fan Jun 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.12

Flavin mononucleotide-binding proteins or domains emit cyan-green fluorescence under aerobic and anaerobic conditions, but relatively low fluorescence and less thermostability limit their application as reporters. In this work, we incorporated the codon-optimized fluorescent protein from Chlamydomonas reinhardtii with two different linkers independently into the redox-responsive split intein construct, overexpressed the precursors in hyperoxic Escherichia coli SHuffle T7 strain, and cyclized the target proteins in vitro in the presence of the reducing agent. Compared with the purified linear protein, the cyclic protein with the short linker displayed enhanced fluorescence. In contrast, cyclized protein with incorporation of the long linker including the myc-tag and human rhinovirus 3C protease cleavable sequence emitted slightly increased fluorescence compared with the protein linearized with the protease cleavage. The cyclic protein with the short linker also exhibited increased thermal stability and exopeptidase resistance. Moreover, induction of the target proteins in an oxygen-deficient culture rendered fluorescent E. coli BL21 (DE3) cells brighter than those overexpressing the linear construct. Thus, the cyclic reporter can hopefully be used in certain thermophilic anaerobes.

Dynamic Task Scheduling Via Policy Iteration Scheduling Approach for Cloud Computing

( Bin Hu ),( Ning Xie ),( Tingting Zhao ),( Xiaotong Zhang ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.3

Dynamic task scheduling is one of the most popular research topics in the cloud computing field. The cloud scheduler dynamically provides VM resources to variable cloud tasks with different scheduling strategies in cloud computing. In this study, we utilized a valid model to describe the dynamic changes of both computing facilities (such as hardware updating) and request task queuing. We built a novel approach called Policy Iteration Scheduling (PIS) to globally optimize the independent task scheduling scheme and minimize the total execution time of priority tasks. We performed experiments with randomly generated cloud task sets and varied the performance of VM resources using Poisson distributions. The results show that PIS outperforms other popular schedulers in a typical cloud computing environment.