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RISS 인기검색어
- 검색키워드
- 저자 : Subbarao, Raghavendra Baregundi (검색결과 17 건)
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Acreage estimation of kharif rice crop using Sentinel-1 temporal SAR data
Subbarao Nandepu V. V. S. S. Teja,Mani Jugal Kishore,Shrivastava Ashish,Srinivas Kumar Samayamantula,Varghese A. O. 대한공간정보학회 2021 Spatial Information Research Vol.29 No.4
Rice is one of the most important food crop in India covering about one-fourth of the total cropped area. India is the second largest producer and consumer of rice and accounts for 21% of the world’s total rice production. Rice is fundamentally a kharif season crop and grown in mainly rainfed areas. Recently there is a considerable increase in production, area and yield of rice crop in India. Temporal monitoring of crop area under cultivation is essential for the sustainable management of agricultural activities on both national and global levels. The present study is envisaged to estimate area under kharif rice using multi-temporal Sentinel-1 Synthetic Aperture Radar (SAR) data with dual polarization (VH and VV) in Bhandara district of Maharashtra. The geographical area of Bhandara district is 4087 square kilometres and lies in between 20640 030 ’ to 21600 180 ’ N latitude and 79440 930 ’ to 80080 700 ’ E longitude. The rice area is extracted using Random Forest (RF) classification techniques available in SNAP tool and validated using the ground observation collected from the field. An area of 1760 square kilometres was found under kharif rice out of 4087 square kilometres area of entire Bhandara district. The rice is predominant crop and covered around 43% of the total geographical area of Bhandara district during kharif season. The user accuracy (omission error), producer accuracy (commission error) for rice crop, overall accuracy and Kappa coefficients were 82.7, 90.0, 91% and 0.80, respectively. The study found that SAR data can be successfully used for acreage estimation with RF classifier.
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Effect of Axial Spacing between the Components on the Performance of a Counter Rotating Turbine
Subbarao, Rayapati,Govardhan, Mukka Korean Society for Fluid machinery 2013 International journal of fluid machinery and syste Vol.6 No.4
Counter Rotating Turbine (CRT) is an axial turbine with a nozzle followed by a rotor and another rotor that rotates in the opposite direction of the first one. Axial spacing between blade rows plays major role in its performance. Present work involves computationally studying the performance and flow field of CRT with axial spacing of 10, 30 and 70% for different mass flow rates. The turbine components are modeled for all the three spacing. Velocity, pressure, entropy and Mach number distributions across turbine stage are analyzed. Effect of spacing on losses and performance in case of stage, Rotor1 and Rotor2 are elaborated. Results confirm that an optimum axial spacing between turbine components can be obtained for the improved performance of CRT.
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Effect of Axial Spacing between the Components on the Performance of a Counter Rotating Turbine
Rayapati Subbarao,Mukka Govardhan 한국유체기계학회 2013 International journal of fluid machinery and syste Vol.6 No.4
Counter Rotating Turbine (CRT) is an axial turbine with a nozzle followed by a rotor and another rotor that rotates in the opposite direction of the first one. Axial spacing between blade rows plays major role in its performance. Present work involves computationally studying the performance and flow field of CRT with axial spacing of 10, 30 and 70% for different mass flow rates. The turbine components are modeled for all the three spacing. Velocity, pressure, entropy and Mach number distributions across turbine stage are analyzed. Effect of spacing on losses and performance in case of stage, Rotor1 and Rotor2 are elaborated. Results confirm that an optimum axial spacing between turbine components can be obtained for the improved performance of CRT.
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Baregundi Subbarao, Raghavendra,Ullah, Imran,Kim, Eun-Jin,Jang, Si-Jung,Lee, Won-Jae,Jeon, Ryoung Hoon,Kang, Dawon,Lee, Sung-Lim,Park, Bong-Wook,Rho, Gyu-Jin MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.5
<P>Endometrial stromal cells (EMSCs) obtained from porcine uterus (<I>n</I> = 6) were positive for mesenchymal stem cell markers (CD29, CD44 and CD90), and negative for epithelial marker CD9 and hematopoietic markers CD34, CD45 analyzed by flow cytometry. Further the cells were positive for expression of mesenchymal markers, <I>CD105</I>, <I>CD140b</I>, and <I>CD144</I> by PCR. Pluripotent markers OCT4, SOX2, and NANOG were positively expressed in EMSCs analyzed by Western blotting and PCR. Further, differentiation into adipocytes and osteocytes was confirmed by cytochemical staining and lineage specific gene expression by quantitative realtime-PCR. Adipocyte (<I>FABP</I>, <I>LPL</I>, <I>AP2</I>) and osteocyte specific genes (<I>ON</I>, <I>BG</I>, <I>RUNX2</I>) in differentiated EMSCs showed significant (<I>p</I> < 0.05) increase in expression compared to undifferentiated control cells. Neurogenic transdifferentiation of EMSCs exhibited distinctive dendritic morphology with axon projections and neuronal specific genes, <I>NFM</I>, <I>NGF</I>, <I>MBP</I>, <I>NES</I>, <I>B3T</I> and <I>MAP2</I> and proteins, B3T, NFM, NGF, and TRKA were positively expressed in neuronal differentiated cells. Functional analysis of neuronal differentiated EMSCs displayed voltage-dependence and kinetics for transient outward K<SUP>+</SUP> currents (<I>I</I><SUB>to</SUB>), at holding potential of −80 mV, Na<SUP>+</SUP> currents and during current clamp, neuronal differentiated EMSCs was more negative than that of control EMSCs. Porcine EMSCs is a suitable model for studying molecular mechanism of transdifferentiation, assessment of electrophysiological properties and their efficiency during <I>in vivo</I> transplantation.</P>
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Human mesenchymal stem cells - current trends and future prospective
Ullah, Imran,Subbarao, Raghavendra ,Baregundi,Rho, Gyu ,Jin Portland Press Ltd. 2015 Bioscience reports Vol.35 No.2
<▼1><P>Stem cells are cells specialized cell, capable of renewing themselves through cell division and can differentiate into multi-lineage cells. These cells are categorized as embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and adult stem cells. Mesenchymal stem cells (MSCs) are adult stem cells which can be isolated from human and animal sources. Human MSCs (hMSCs) are the non-haematopoietic, multipotent stem cells with the capacity to differentiate into mesodermal lineage such as osteocytes, adipocytes and chondrocytes as well ectodermal (neurocytes) and endodermal lineages (hepatocytes). MSCs express cell surface markers like cluster of differentiation (CD)29, CD44, CD73, CD90, CD105 and lack the expression of CD14, CD34, CD45 and HLA (human leucocyte antigen)-DR. hMSCs for the first time were reported in the bone marrow and till now they have been isolated from various tissues, including adipose tissue, amniotic fluid, endometrium, dental tissues, umbilical cord and Wharton's jelly which harbours potential MSCs. hMSCs have been cultured long-term in specific media without any severe abnormalities. Furthermore, MSCs have immunomodulatory features, secrete cytokines and immune-receptors which regulate the microenvironment in the host tissue. Multilineage potential, immunomodulation and secretion of anti-inflammatory molecules makes MSCs an effective tool in the treatment of chronic diseases. In the present review, we have highlighted recent research findings in the area of hMSCs sources, expression of cell surface markers, long-term <I>in vitro</I> culturing, <I>in vitro</I> differentiation potential, immunomodulatory features, its homing capacity, banking and cryopreservation, its application in the treatment of chronic diseases and its use in clinical trials.</P></▼1><▼2><P>In this review, we highlighted recent research findings in the area of human mesenchymal stem cells, its application in the treatment of chronic diseases and its use in human clinical trials.</P></▼2>
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Ock, Sun-A,Baregundi Subbarao, Raghavendra,Lee, Yeon-Mi,Lee, Jeong-Hyeon,Jeon, Ryoung-Hoon,Lee, Sung-Lim,Park, Ji Kwon,Hwang, Sun-Chul,Rho, Gyu-Jin Hindawi Publishing Corporation 2016 Stem cells international Vol.2016 No.-
<P>Mesenchymal stromal/stem cells (MSCs) demonstrate immunomodulation capacity that has been implicated in the reduction of graft-versus-host disease. Accordingly, we herein investigated the capacity of MSCs derived from several tissue sources to modulate both proinflammatory (interferon [IFN] <I>γ</I> and tumor necrosis factor [TNF] <I>α</I>) and immunosuppressive cytokines (transforming growth factor [TGF] <I>β</I> and interleukin [IL] 10) employing xenogeneic human MSC-mixed lymphocyte reaction (MLR) test. Bone marrow-derived MSCs showed higher self-renewal capacity with relatively slow proliferation rate in contrast to adipose-derived MSCs which displayed higher proliferation rate. Except for the lipoprotein gene, there were no marked changes in osteogenesis- and adipogenesis-related genes following in vitro differentiation; however, the histological marker analysis revealed that adipose MSCs could be differentiated into both adipose and bone tissue. TGF<I>β</I> and IL10 were detected in adipose MSCs and bone marrow MSCs, respectively. However, skin-derived MSCs expressed both IFN<I>γ</I> and IL10, which may render them sensitive to immunomodulation. The xenogeneic human MLR test revealed that MSCs had a partial immunomodulation capacity, as proliferation of activated and resting peripheral blood mononuclear cells was not affected, but this did not differ among MSC sources. MSCs were not tumorigenic when introduced into immunodeficient mice. We concluded that the characteristics of MSCs are tissue source-dependent and their in vivo application requires more in-depth investigation regarding their precise immunomodulation capacities.</P>
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DMSO‐ and Serum‐Free Cryopreservation of Wharton's Jelly Tissue Isolated From Human Umbilical Cord
Shivakumar, Sharath Belame,Bharti, Dinesh,Subbarao, Raghavendra Baregundi,Jang, Si‐,Jung,Park, Ji‐,Sung,Ullah, Imran,Park, Ji‐,Kwon,Byun, June‐,Ho,Park, Bong‐,Wook,Rho, G John Wiley and Sons Inc. 2016 Journal of cellular biochemistry Vol.117 No.10
<P><B>ABSTRACT</B></P><P>The facile nature of mesenchymal stem cell (MSC) acquisition in relatively large numbers has made Wharton's jelly (WJ) tissue an alternative source of MSCs for regenerative medicine. However, freezing of such tissue using dimethyl sulfoxide (DMSO) for future use impedes its clinical utility. In this study, we compared the effect of two different cryoprotectants (DMSO and cocktail solution) on post‐thaw cell behavior upon freezing of WJ tissue following two different freezing protocols (Conventional [−1°C/min] and programmed). The programmed method showed higher cell survival rate compared to conventional method of freezing. Further, cocktail solution showed better cryoprotection than DMSO. Post‐thaw growth characteristics and stem cell behavior of Wharton's jelly mesenchymal stem cells (WJMSCs) from WJ tissue cryopreserved with a cocktail solution in conjunction with programmed method (Prog‐Cock) were comparable with WJMSCs from fresh WJ tissue. They preserved their expression of surface markers, pluripotent factors, and successfully differentiated in vitro into osteocytes, adipocytes, chondrocytes, and hepatocytes. They also produced lesser annexin‐V‐positive cells compared to cells from WJ tissue stored using cocktail solution in conjunction with the conventional method (Conv‐Cock). Real‐time PCR and Western blot analysis of post‐thaw WJMSCs from Conv‐Cock group showed significantly increased expression of pro‐apoptotic factors (BAX, p53, and p21) and reduced expression of anti‐apoptotic factor (BCL2) compared to WJMSCs from the fresh and Prog‐Cock group. Therefore, we conclude that freezing of fresh WJ tissue using cocktail solution in conjunction with programmed freezing method allows for an efficient WJ tissue banking for future MSC‐based regenerative therapies. J. Cell. Biochem. 117: 2397–2412, 2016. © 2016 The Authors. <I>Journal of Cellular Biochemistry</I> published by Wiley Periodicals, Inc.</P>
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Shrivastav Awani,Goswami Tridib K.,Kotra Subbarao V. 한국농업기계학회 2022 바이오시스템공학 Vol.47 No.3
Purpose The freezing technique and rheological properties play an important role in improving the viscoelastic as well as the textural properties of ice cream. Methods Viscoelastic properties of ice creammix and ice creamwere measured by varying the concentration of gelatin (from0.2 to 0.6%) using the oscillatory test at different frequencies. The rheological analysis was carried out at temperatures 4 °C and − 4 °C which is the temperature of ice cream, before and after carrying out the freezing process, respectively. In ice creammix and ice cream, the values of storage modulus (G′), loss modulus (G″), and tan δ (G″/G′) were calculated from 0.2 to 0.6% gelatin concentration which was prepared by liquid nitrogen (LN2) infusion method. The adaptive neuro-fuzzy logic approach was applied to test the validity of sensory scores obtained in terms of the overall acceptability of ice cream. Results It was found that the viscoelastic property of ice cream was significantly influenced (p ≤ 0.01) by increasing the concentration of gelatin in ice cream and can be successfully added up to the concentration level of 0.4%, beyond which it results in the stiffness of ice cream reducing the quality of ice cream. Also, it was found that the average percentage error in the present ANFIS model was 5.85% with a correlation coefficient of 0.866 which indicates a good amount of correlation between predicted and experimental values. Conclusion The results of this study would be helpful in the further study of quick freezing of ice cream by LN2 infusion method by varying stabilizer concentration which is getting more common nowadays in western countries.
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