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Pushpendra Singh,Manish Kumar Tripathi,Mohammad Yasir,Ashish Ranjan,Rahul Shrivastava 환경독성보건학회 2021 환경독성보건학회지 Vol.36 No.3
Methyl isocyanate (MIC), a low molecular weight synthetic aliphatic compound, having an isocyanate group (−NCO), has industrial application. In this study, the effects of methyl isocyanate and its mechanism on outer membrane protein of Escherichia coli were observed using experimental and computational methods. In vitro exposure of N-succinimidyl N-methylcarbamate (NSNM) a synthetic analogue of MIC on E. coli to a final concentration of 2 mM was found to affect the growth curve pattern and changes in cell morphology. Molecular docking studies of MIC and NSNM with E. coli outer membrane protein (OmpW, OmpX, OmpF OmpA), and periplasmic domain (PAL) were performed. The in-silico results revealed that outer membrane protein OmpF showed the highest negative binding energy, i.e. ΔG -4.11 kcal/mole and ΔG -3.19 kcal/mole by NSNM and MIC as compared to other proteins. Our study concludes that methyl isocyanate retains lethal toxicity which leads to cell death due to the membrane protein damage of E. coli membrane.
Acreage estimation of kharif rice crop using Sentinel-1 temporal SAR data
Subbarao Nandepu V. V. S. S. Teja,Mani Jugal Kishore,Shrivastava Ashish,Srinivas Kumar Samayamantula,Varghese A. O. 대한공간정보학회 2021 Spatial Information Research Vol.29 No.4
Rice is one of the most important food crop in India covering about one-fourth of the total cropped area. India is the second largest producer and consumer of rice and accounts for 21% of the world’s total rice production. Rice is fundamentally a kharif season crop and grown in mainly rainfed areas. Recently there is a considerable increase in production, area and yield of rice crop in India. Temporal monitoring of crop area under cultivation is essential for the sustainable management of agricultural activities on both national and global levels. The present study is envisaged to estimate area under kharif rice using multi-temporal Sentinel-1 Synthetic Aperture Radar (SAR) data with dual polarization (VH and VV) in Bhandara district of Maharashtra. The geographical area of Bhandara district is 4087 square kilometres and lies in between 20640 030 ’ to 21600 180 ’ N latitude and 79440 930 ’ to 80080 700 ’ E longitude. The rice area is extracted using Random Forest (RF) classification techniques available in SNAP tool and validated using the ground observation collected from the field. An area of 1760 square kilometres was found under kharif rice out of 4087 square kilometres area of entire Bhandara district. The rice is predominant crop and covered around 43% of the total geographical area of Bhandara district during kharif season. The user accuracy (omission error), producer accuracy (commission error) for rice crop, overall accuracy and Kappa coefficients were 82.7, 90.0, 91% and 0.80, respectively. The study found that SAR data can be successfully used for acreage estimation with RF classifier.