웹기반의 가축전염병 발생자료 관리시스템(AIMS) 개발

박병호,김석재,황인진,김시동,남향미,위성환 한국농업정보과학회 2003 농업정보과학 Vol.4 No.1

The AIMS(Anima1 Infectious Disease Data Management System) was developed to collect and analyze data on animal infectious disease outbreak (62 diseases). When an animal infectious disease is confirmed at a diagnostic laboratory, the disease outbreak is then reported to the regional government through AIMS. After the regional government confirms the number of animals infected or dead, outbreak date and etc, this information is reported to the Ministry of Agriculture & Forestry and the National Veterinary Research & Quarantine Service through AIMS. The AIMS analyzes the outbreak data and reports the outbreak statistics by region, month and year to them. In addition, the AIMS can identify the farm where there was an outbreak of the animal infectious disease. There are four user roles : 1) Administrator, responsible for the overall operation of the system. 2) Inspector, responsible for confirmation of the animal infectious disease outbreak. 3) Pathologist, responsible for input to an animal infectious disease at first. 4) Gallery, only able to read the outbreak statistics. The AIMS has been implemented using the ASP technology and is operated on the web to allow easy access. The data, middle and presentation tier are based on MS-SQL 2000 server, Microsoft transaction server and Internet Explorer, respectively. The database's stored procedure was used to improve the performance. In addition to above software, the dextupload software is used to upload the files. By using the AIMS, a more accurate statistics for the animal infectious disease outbreak is expected.

Microalgae, Tetraselmis tetrathele has Alopecia Prevention and Scalp Improvement

( Si-hyang Park ),( Kyong-dong Lee ),( Ginnae Ahn ),( Hye-jin Park ),( Kap Seong Choi ),( Jiyeon Chun ),( Sun-yup Shim ) 한국미생물 · 생명공학회 2021 한국미생물·생명공학회지 Vol.49 No.4

The microalga, Tetrathelmis tetrathele, is used in the development of products for the aquaculture, food, and nutraceutical industries. In the present study, we investigated whether the T. tetrathele ethanolic extract (TTE), which has anti-inflammatory properties, can confer protection against alopecia and improve scalp health, influence the proliferation of human keratinocytes, HaCaT cells, and human hair follicle dermal papilla cells (HFDPC), or inhibit 5α-reductase activity. We found that TTE inhibited the production of the inflammatory mediator, nitric oxide (NO), and prostaglandin E₂ (PGE₂) without cytotoxicity in LPS-stimulated RAW 264.7 cells. In addition, TTE encouraged the proliferation of HaCaT cells and HFDPC. Our results showed that TTE had anti-inflammatory activities, proliferated HaCaT cells and HFDPC, and inhibited 5α-reductase activity. Therefore, we suggest that T. tetrathele could be a potent therapeutic agent for alopecia prevention and scalp improvement.

[P8-104] Antioxidative Effects of Mugwort (Artemisia vulgaris ᄂ.) Extracts Diet on ICR Mouse Skin

Si-Hyang Park,Duck-Moon Cho,Gyeonglim Choi,Yeung Joon Choi,Jin-Ho Choi 한국식품영양과학회 2007 한국식품영양과학회 학술대회발표집 Vol.2007 No.10

아세트아미노펜 유도 HepG-2 세포주 손상에 대한 굴 효소 가수분해물의 보호 효과

박시향(Si-Hyang Park),문성실(Sung-Sil Moon),Cheng-Liang Xie,정세영(Se-Young Choung),최영준(Yeung-Joon Choi) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.8

본 연구는 굴 가수분해물이 아세트아미노펜에 의한 간독성의 무독화 효과를 HepG-2 세포를 사용하여 조사하였다. 굴 가수분해물은 굴 단백질의 가교연결을 위해 TGase로 전처리하거나(TGPN) 혹은 하지 않고(PN), 1% Protamex와 1% Neutrase 단백질 분해효소로 2단 가수분해하였다. 두 종류의 굴 가수분해물은 아세트아미노펜으로 간 손상을 유도한 세포에 각각 처리하여 세포 생존율을 측정하였으며, 세포 배양 시 배양액으로 유출된 GOT와 GPT 활성을 측정하였다. TGPN 가수분해물의 경우 아세트아미노펜만을 처리한 negative 대조군(60.7±3.2%)에 비하여 100 μg/mL와 200 μg/mL의 농도에서 각각 136.2±1.4%와 179.6±3.8%의 높은 세포 생존율을 보였다. PN 가수분해물은 100 μg/mL와 200 μg/mL의 농도에서 각각 107.9±8.8%와 130.6±7.6%의 세포 생존율을 보였다. GOT 활성은 negative 대조군의 경우에 38.3±0.2 Karmen/mL이었으며, TGPN(200μg/mL)과 PN(200 μg/mL)에서는 19.9±0.5와 22.0±2.4 Karmen/mL로 농도에 따라 유의적인 활성 감소를 확인할 수 있었다. 그리고 GPT 활성도 GOT와 같은 경향의 활성을 나타내었다. 이 같은 결과에 미루어 굴 유래 가수분해물의 간 보호 건강 기능 식품 혹은 약물 개발의 가능성을 확인하였으며 앞으로 가수분해 펩티드 중의 유효성분에 대한 구조동정과 작용 기전에 관한 연구가 필요할 것으로 보인다. This study investigated the detoxification effects of enzymatic hydrolysate from oyster on acetaminophen-induced toxicity using HepG-2 cells. Oyster hydrolysate was made with 1% Protamex and 1% Neutrase after treatment with transglutaminase (TGPN) or without (PN). Two types of oyster hydrolysate were added to human-derived HepG-2 hepatocytes damaged by acetaminophen, after which the survival rate of HepG-2 cell was measured. In addition, glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) activities in the culture media were evaluated. The survival rates of HepG-2 cells were 136.2±1.4% at 100 μg/mL of TGPN and 179.6±3.8% at 200 μg/mL of TGPN. These cell survival rates were higher compared to that of the negative control group (60.7±3.2%) treated only with acetaminophen. GOT activity was 38.3±0.2 Karmen/mL in the negative control group, whereas it was 19.9±0.5 for TGPN (200 μg/mL) and 22.0±2.4 Karmen/mL for PN (200 μg/mL). GOT and GTP activities were shown to be dependent on TGPN concentration, and significant reduction in activities could be conformed. The detoxification efficacy of TGPN was higher compared to that of PN. These results suggest that oyster hydrolysate has potential as a healthy food or pro-drug for liver protection.

자외선 조사 무모쥐 피부조직에 도포한 애엽(Mugwort) 추출물의 주름개선 효과

박시향(Si-Hyang Park),홍유미(Yumi Hong),최영준(Yeung Joon Choi),최진호(Jin-Ho Choi),김병관(Byung-Kwan Kim) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.9

무모쥐의 등에 애엽 추출물과 아스코르브산 함유 스킨로션을 도포하고 자외선을 조사하여 애엽 추출물과 아스코르브산의 주름 개선 효과를 알아보았다. 애엽 추출물 도포군의 피부조직 두께는 대조군에 비해 12.5~21.4%의 유의적인 감소 효과를 보였다. 표면 거칠기 측정기에 의한 피부 주름의 형성 정도를 측정하는 지표인 Ra값은 ME-1.0, ME-2.0과 ME-5.0 도포군에서 23.7~31.1%로 유의적으로 감소하였고 (p<0.01), Rq치는 ME-1.0, ME-2.0와 ME-5.0에서 11.2~21.2%, Rz의 측정치에서는 ME-1.0(19.8%), ME-2.0(22.1%), ME-5.0(24.5%) 그룹에서 대조군에 비해 크게 감소하였다. Rt치는 ME-1.0, ME-2.0와 ME-5.0 그룹에서 농도 증가에 따라 14.2%에서 22.7%의 유의적인 감소효과가 있었다. 아스코르브산 도포그룹은 애엽과 비슷한 감소효과를 보여주었지만, 애엽의 주름 개선효과에는 미치지 못하였다. MMP-1 활성은 애엽 메탄올 추출물 그룹에서 19.3%~22.6%까지 감소하였고, 콜라겐의 생성은 ME-2.0과 ME-5.0그룹에서 10% 정도의 유의적인 증가 효과가 있었다. 이처럼 애엽 메탄올 추출물은 피부의 광손상에 대한 피부 보호 및 피부의 주름 개선효과가 탁월하고, 또한 강력한 항산화제인 아스코르브산보다 그 효과가 우수하여 피부노화 현상과 주름 개선을 위한 기능성 화장품 소재로 이용가치가 있을 것으로 여겨진다. This study was to investigate antiwrinkle effect of mugwort (Artemisia vulgaris) methanol extract in hairless mouse skin induced by UVB-irradiation. Hairless mouse were topically treated with the basic lotion alone (control), ascorbic acid (AA-0.5%, AA-1.0%, AA-2.0%, and AA-5.0%) and mugwort extract (ME-0.5%, ME-1.0%, ME-2.0%, and ME-5.0%) dissolved in a basic lotion. After topical treatment of 30 minutes, the animals were irradiated with increasing doses of UVB radiation (60~100 mJ/㎠) for 4 weeks. In our experimental condition, skin thickness of hairless mouse was significantly decreased (12.5~21.4%) in all ME groups compared with control group. Ra value, that is surface roughness parameter induced by skin wrinkling, was significantly decreased (23.7~31.1%) in ME-1.0%, 2.0% and 5.0% group compared with control group. Furthermore, Rq, Rz and Rt value were significantly decreased to 11.2~21.2%, 19.8%~24.5%, and 14.2%~22.7%, respectively. Wrinkle formation of ascorbic acid treatment group as reference group was inhibited, but its effect was less than ME treatment. Matrix metalloproteinase-1 activity was significantly inhibited (19.7~22.6%) compared with control group and collagen content was significantly increased (about 10%) when compared with control group. These results indicate that ME could protect skin aging and wrinkle formation in hairless mouse from photo-irradiation.

자외선 조사 무모쥐 피부에 도포한 애엽(Mugwort) 추출물의 항산화 효과

박시향(Si-Hyang Park),조득문(Duck-Moon Cho),최병대(Byeong-Dae Choi),최영준(Yeung Joon Choi),최진호(Jin-Ho Choi) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.1

무모쥐의 피부에 도포한 애엽 추출물과 positive 비교군인아스코르브산이 피부 조직에 미치는 항산화효과를 조사하였다. 활성산소인 과산화수소의 생성량은 애엽 추출물과 아스코르브산 2%와 5% 도포군에서 유의적으로 감소하였다. 히드록시 라디칼의 생성량은 애엽 추출물 2%와 5% 도포군에서만 유의적인 감소효과가 있었다. 산화적 스트레스의 지표인 산화 단백질의 함량은 모든 애엽 추출물 도포군에서 대조군에 비해 유의적으로 감소(14.6%~18.5%)하였으나, 아스코르브산의 경우는 2% 도포군에서만 저해효과가 인지되었다. 과산화지질 생성량은 대조군에 비해 애엽 추출물과 아스코르브산 도포그룹 모두에서 크게 감소하였다. 항산화효소인 카탈라아제의 활성은 애엽 추출물과 아스코르브산 모든 그룹에서 첨가 농도가 증가함에 따라 크게 증가하였으며, 글루타치온 퍼옥시다아제의 활성은 애엽 추출물 도포군은 2%와 5%에서, 아스코르브산의 경우는 모든 그룹에서 유의적인 활성 증가를 보였다. 이상의 결과는 피부 노화 억제기능성 화장품 소재로서 애엽 추출물의 활용 가능성을 제시한다. This study investigated the antioxidative effect of mugwort (Artemisia vulgaris L.) extracts in hairless mouse skin from oxidative stress induced by UV-irradiation. After topical application on hairless mouse back with basic skin lotion group (control), ascorbic acid group (AA-0.5%, AA-1.0%, AA-2.0%, and AA-5.0%), and mugwort extract group (ME-0.5%, ME-1.0%, ME-2.0%, and ME-5.0%), the animals were irradiated to increasing doses of UVB (60 mJ~100 mJ) for 4 weeks. Hydrogen peroxide of hairless mouse skin homogenate significantly decreased in 2% (p<0.05) and 5% (p<0.05) of ME and AA groups. Hydroxyl radicals were decreased significantly in both of 2% and 5% ME groups as compared to AA groups (p<0.05). Oxidative stress levels deduced by oxidized protein contents were greatly decreased (14.6~18.5%) in all ME treatment groups, while only at 2% of AA treatment group. Lipid peroxide contents were greatly inhibited in all ME and AA treatment groups (p<0.01). Application of ME significantly increased catalase activity, over 25% in all mugwort and AA groups. Glutathione peroxidase activities were increased up to 20.5%~32.8% in 2.0% and 5% ME groups, whereas it increased in all AA groups. These results suggested that mugwort extract was more effective than that of ascorbic acid in protecting hairless mouse skin from photo-irradiation, and can be used as an potential anti-aging cosmetic ingredients.

애엽 추출물 섭취가 ICR 마우스의 피부조직에 미치는 항산화 효과

박시향(Si?Hyang Park),조득문(Duck?Moon Cho),최경림(Gyeonglim Choi),최영준(Yeung Joon Choi),최진호(Jin?Ho Choi) 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.12

애엽 추출물에 대한 ICR 마우스의 피부 조직에 대한 항산화작용 및 산화적 스트레스에 미치는 영향을 조사하였다. 애엽 추출물(mugwort extract: ME)을 섭취한 식이군의 경우, 추출물의 농도에 따라 피부조직의 단백질 함량이 대조군에 비해 3.1%~11.1% 증가하였다. 활성산소 중 히드록시 라디칼 함량은 애엽 추출물 식이군에서 10.4%~17.4%의 유의적인 감소 효과가 인정되었으며, 그리고 산화적 스트레스로써 산화단백질의 함량도 애엽 추출물 식이군(ME-100, ME-200)이 대조군에 비해 각각 15.2%와 17.1%의 유의적인 감소를 보였다(p<0.05). 과산화지질함량은 애엽 추출물의 농도증가에 따라 감소되는 경향을 보여주었으나, 통계적인 유의성은 없었다. 수퍼옥시드 디스무타아제 활성은 ME-100 군과 ME-200 군에서 각각 15.0%와 23.3%의 유의적인 증가가 있었고, 또한 애엽 추출물의 농도가 증가할수록 카탈라아제 활성도 증가하는 것으로 나타났다. 애엽 추출물의 섭취가 피부의 활성산소를 제거하고 산화적 스트레스를 억제하며, 항산화효소의 활성을 증가시켜 피부의 노화현상을 억제시키는 효과가 있음을 확인할 수 있었다. 강력한 항산화제인 아스코르브산과 비교하여도 손색이 없는 높은 활성을 보였다. 이와 같은 결과는 피부 건강기능 식품과 음료 소재로서 애엽의 이용 가능성을 제시한다. The feeding effects of mugwort (Artemisia vulgaris L.) extracts (ME) on the anti-oxidative actions of ICR mouse skin was investigated. To study the antioxidative effects of ME on ICR mouse skin, female ICR mice were grouped into basic diet group (control), ascorbic acid diet group (AA-2.5, AA-5.0, AA-10.0 and AA-20.0 ㎎/㎏ BW/day) as a positive control and experimental diet group (mugwort extract; ME-25, ME-50, ME-100, and ME-200 ㎎/㎏ BW/day) and fed for 10 weeks. Protein contents in ME-50, ME-100, and ME-200 feeding group were increased (3.1%~11.1%) and hydroxyl radical contents were significantly decreased (10.4%~17.4%) compared to control group. Oxidative stress signals and oxidized protein contents were significantly reduced to the range of 15.3 to 17.1% in ME-100 and ME-200 groups. Also, superoxide dismutase (SOD) activity was significantly increased to the range of 15.0% to 23.3% in ME-100 and ME-200 groups. Catalase activities were significantly increased (14.0%~36.9%) in all groups in a dose-dependent pattern. Antioxidative ability of ME showed similarity to that of ascorbic acid.

고령의 4차 관해를 이룬 급성 골수성 백혈병 환자에서 non-myeloablative 전처치를 이용한 동종 말초 혈액 조혈모 세포 이식 1예

박재홍,정시욱,서향은,전재현,신동건,이정림,정진태,김종광,손상균,이규보 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.1

Standard stem cell transplantation produces considerable morbidity and mortality because of toxicities due to the high dose conditioning regimen. Therefore, this approach must be restricted to young patients without cormobidities. The majority of patients with hematologic malignancies are not eligible for high dose conditioning regimen because of older age or cormobid conditions. An alternate strategy is to use a less intensive preparative regimen designed not to eradicate the malignancy but to provide sufficient immunosuppression to achieve engraftment of an allogeneic blood stem cell or marrow graft, thus allowing the development of an immune GVM (graft-versus-malignancy) effect. The main therapeutic effect in non-myeloablative allogeneic stem cell transplantation is due to the graft-versus-leukimia(GVL) effect which eliminate residual or recurrent leukemia. We report a 56 years old AML patient in 4th complete remission who successfully treated with allogeneic PBSCT with non-myeloablative conditioning regimen.

메를로 퐁티의 몸 철학과 환경교육의 방향

서향숙(Hyang-suk Seo),박시윤(Si-yun Park) 한국환경교육학회 2014 環境 敎育 Vol.27 No.3

이 글은 현대사회에서 인간위기를 포함한 환경위기를 극복하기 위한 철학적 대안을 메를로 퐁티의 ‘몸의 현상학’에서 찾아, 우리 미래의 삶이 자연과의 공생으로 가능하게 하는데 목적이 있다. 근대 베이컨의 유토피아적 과학관과 데카르트의 합리적 전통의 결합으로 누려온 현대 과학 기술 문명의 혜택 사이에 실존주의 철학이 있다. 1990년대 후반 ‘몸’이 인문, 사회과학적 담론의 핵심 키워드로 작용하던 포스트모더니즘의 구도에서 샤르트르와 함께 20세기 몸의 철학으로 새로운 지평을 연 메를로 퐁티의 ‘살 존재론’을 통해 이성중심의 과학주의가 일으키는 부작용을 근본적으로 한계 지을 수 있는 사유의 단초를 다음과 같이 마련하고자 한다. 첫째, 현대 과학 기술 문명에 내재된 여러 위험적 징후들을 철학과 과학계의 비판적 시각을 중심으로 살펴보고자 한다. 둘째, 과학 기술 토대위에 오랜 시간 혜택만 누려온 인간이 과연 세계의 중심이 될 수 있는가?에 대한 물음의 답을 메를로 퐁티의 현상학, 몸의 철학을 중심으로 찾아보고자 한다. 셋째, 그의 ‘살 존재론’을 근거로 살아있는 모든 것에 대한 감수성을 키우는 방법이 곧 미래의 우리 삶과 자연과의 공생을 가능하게 할 것이라는 주장을 하고자 한다. The purpose of this study was to make human life in the future be able to coexist with nature by seeking for a philosophical alternative to overcome environmental crisis including human crisis in modern society from Mearleau-Ponty’s ‘phenomenology of the body’. Existentialist philosophy is placed somewhere in the benefits of the modern scientific and technological civilization, which is enjoyed by the combination of Bacon’s utopian viewpoint of science and Descartes’ rational tradition. Based on ‘the flesh ontology’ of Mearleau-Ponty who opened a new prospect with Sartre through the philosophy of the body in the 20th century, under the post-modernism structure in the latter part of the 1990s of which core keyword for the discourse of human and social science was ‘the body’, the author tried to find out a clue of thinking that could clarify the side effect of rationality-oriented scientism as follows: First, a variety of dangerous signs of modern scientific and technological civilization was examined focusing on the critical view of philosophical and scientific circles. Second, the answer to the question ‘Is it possible for human beings who have merely enjoyed the benefits of science and technology for a long time to be the center of the world?’ was looked into focusing on Mearleau-Ponty’ phenomenology and body philosophy. Third, based on Mearleau-Ponty’ ‘flesh ontology’, the author argued that cultivating sensitivity for all the living things seems to be the clue for the symbiosis between human life and nature in the future.

다중가스센서를 이용한 화재의 조기검출에 대한 연구

조시형 ( Si Hyung Cho ),장향원 ( Hyang Won Jang ),전진욱 ( Jin Wook Jeon ),최석임 ( Seok Im Choi ),김선규 ( Sun Gyu Kim ),강종위 ( Zhong Wei Jiang ),최삼진 ( Sam Jin Choi ),박찬원 ( Chan Won Park ) 한국센서학회 2014 센서학회지 Vol.23 No.5

This paper introduced a novel multi-gas sensor detector with simple signal processing algorithm. This device was evaluated by investigating the characteristics of combustible materials using fire-generated smell and smoke. Plural sensors including TGS821, TGS2442, and TGS260X were equipped to detect carbon monoxide, hydrogen gas, and gaseous air contaminants which exist in cigarette smoke, respectively. Signal processing algorithm based on the difference of response times in fire-generated gases was implemented with early and accurately fire detection from multiple gas sensing signals. All fire experiments were performed in a virtual fire chamber. The cigarette, cotton fiber, hair, polyester fiber, nylon fiber, paper, and bread were used as a combustible material. This analyzing software and sensor controlling algorithm were embedded into 8-bit micro-controller. Also the detected multiple gas sensor signals were simultaneously transferred to the personnel computer. The results showed that the air pollution detecting sensor could be used as an efficient sensor for a fire detector which showed high sensitivity in volatile organic compounds. The proposed detecting algorithm may give more information to us compared to the conventional method for determining a threshold value. A fire detecting device with a multi-sensor is likely to be a practical and commercial technology, which can be used for domestic and office environment as well as has a comparatively low cost and high efficiency compared to the conventional device.