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Park, Seunghye,Nguyen, Thu Ha Thi,Jin, EonSeon Elsevier Applied Science 2019 Bioresource Technology Vol. No.
<P><B>Abstract</B></P> <P>Over the past decade, the number of original articles and reviews presenting microalgae as a promising feedstock for biodiesel has increased tremendously. Many improvements of microalgae have been achieved through selection and strain development for industrial applications. However, the large-scale production of lipids for commercialization is not yet realistic because the production is still much more expensive than that of agricultural products. This review summarizes recent research on the induction of lipid biosynthesis in microalgae and the various strategies of genetic and metabolic engineering for enhancing lipid production. Strain engineering targets are proposed based on these strategies. To address current limitations of strain engineering for lipid production, this review provides insights on recent engineering strategies based on molecular tools and methods, and also discusses further perspectives.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Induction of lipid biosynthesis in microalgae is reviewed. </LI> <LI> Comprehensive strategies of strain improvement for lipid production are summarized. </LI> <LI> Targets for enhancing lipid productivity in microalgae are suggested. </LI> <LI> Challenges and perspectives of strain development for lipid production are discussed. </LI> </UL> </P>
Park, Seunghye,Lee, Yew,Jin, EonSeon The Korean Society of Phycology 2013 ALGAE Vol.28 No.2
Dunaliella salina and Dunaliella bardawil are well known for carotenogenesis, the overproduction of carotenoids, under stress conditions. The effect of high light (HL) and low light (LL) on the growth, morphology, photosynthetic efficiency, and the ${\beta}$-carotene and zeaxanthin production of D. salina CCAP 19/18 and D. bardawil was investigated and compared. Both strains showed similar growth kinetics under LL growth condition, but D. salina CCAP 19/18 was faster. As the light intensity increased, D. salina CCAP 19/18 cells were elongated and D. bardawil cells became larger. Both strains showed decrease of the maximum quantum yield of PSII ($F_v/F_m$) and election transport rate (ETR) under HL growth condition and D. salina CCAP 19/18 was less liable to the light stress. Both strains had about 1.8 and 5 times difference in the $O_2$ evolution rate at LL and HL conditions, respectively. The ${\beta}$-carotene and zeaxanthin production were increased as the light intensity increased in both strains. D. bardawil was more sensitive to light intensity than D. salina CCAP 19/18. The possible application of D. salina CCAP 19/18 as a carotenogenic strain will be discussed.