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Seungeun Baek,Thanh‑Tam Ho,Hyoshin Lee,Gooyoung Jung,김영은,Cheol‑Seung Jeong,박소영 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1
Centella asiatica contains various triterpenoids, including madecassoside, asiaticoside, madecassic acid, and asiatic acid. Among them, they are mainly contained in leaf, and among them asiaticoside is known as tissue-specific compound that are mainly accumulated in the leaves. Therefore, in the present study, C. asiatica hairy root cultures were established from petiole and leaf explants using Agrobacterium rhizogenes-mediated transformation. To promote the biosynthesis of these triterpenoids, hairy root cultures were treated with precursors (squalene and pyruvic acid) and elicitors [methyl jasmonate (MeJA) and salicylic acid (SA)] at various concentrations. No significant difference was detected in the growth index of hairy root cultures between precursor feeding and elicitation, except pyruvic acid feeding, suggesting that elicitors do not affect the growth of hairy roots. Treatment with 2.5 mM squalene and 5 mM pyruvic acid enhanced the biosynthesis of target triterpenoids (57.53 and 29.13 mg g−1 DW, respectively) compared with other treatments. However, compared to squalene, MeJA showed a greater effect on the triterpene saponin content in C. asiatica. The highest quantity of triterpenoids (60.25 mg g−1 DW) was produced in hairy root cultures treated with 400 μM MeJA. These results indicate that precursorand elicitor-treated C. asiatica hairy root cultures rapidly accumulate biomass and could be used to enhance the production of triterpenoids for industrial purposes.
나노초 레이저의 표면 처리를 이용한 SM490A 젖음성 변화 연구
백승은(Seungeun Baek),서영진(Youngjin Seo),박동규(Dongkyu Park),Mang Muan Lian,Feyisola Idowu Nana,Tam Van Huynh,성민철(Mincheoul Seong),전수빈(Subin Jeon),Suman Chatterjee,이동경(Dongkyoung Lee) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.11
According to the power of the nanosecond laser, the SM490A surface is irradiated in a grid pattern. After laser irradiation, the microstructure appears at a power of more than 6 W. At 6 W, relatively uniform grid pattern microstructures appear, but as laser power increases, the microstructure changes due to the high thermal effect. When the contact angle is measured with time, superhydrophilicity appears immediately after laser irradiation, but the contact angle increases with time. It becomes hydrophobic in 10~15 days, and a contact angle of 140° at 6 W appears in 30 days. When the contact angle is observed according to power, hydrophilicity appears at 2 W, 4 W without microstructure. Hydrophobicity appears at power above 6 W with microstructures. The highest contact angle appears at 6W with uniform microstructure.
Pae, Munkyong,Baek, Yunjung,Lee, Seungeun,Wu, Dayong Elsevier 2018 The Journal of nutritional biochemistry Vol.57 No.-
<P><B>Abstract</B></P> <P>Loss of ovarian function, as occurs in menopause or after ovariectomy (OVX), is associated with insulin resistance. Adipose tissue inflammation is suggested to be a key component of obesity-induced insulin resistance in male rodents. However, little is known about the effect of OVX and diet on insulin resistance in association with immune homeostasis. Thus, we conducted this study to determine how high-fat diet (HFD) and OVX, alone or in combination, impacted adipose tissue inflammation and insulin resistance. Nine-week-old sham and OVX-treated C57Bl/6 mice were fed low-fat diet (LFD) or HFD (60%) up to 16 weeks. Glucose metabolism was assessed, and adipose tissue and spleen were characterized for tissue inflammation and immune cell populations. First, we found that HFD induced glucose intolerance in both OVX mice and, to a lesser extent, sham mice. OVX mice fed LFD showed no difference in glucose intolerance compared to sham mice. Additionally, OVX mice only when exposed to HFD displayed a proinflammatory profile in adipose tissue: increased macrophages together with dominant M1-like phenotype and also increased T cells, B cells and NK cells compared to those with intact ovarian function. Together, our findings indicate that loss of ovarian function coupled with an HFD intake promotes insulin resistance and adipose tissue inflammation by disturbing adipose tissue immune homeostasis. These findings have a clinical implication in the dietary guidance for menopausal women.</P>