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( Seong Taeck Kim ),( Jae Woong Koh ) 대한안과학회 2011 Korean Journal of Ophthalmology Vol.25 No.3
Purpose: The purpose of this study is to understand the mechanism of apoptosis occurring on a cultured human lens epithelial cell line after exposure to ultraviolet (UV) light. We intended to confirm the presence of cellular toxicity and apoptosis and to reveal the roles of p53, caspase 3 and NOXA in these processes. Methods: Cells were irradiated with an ultraviolet lamp. Cellular toxicity was measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Hoechst staining and fluorescent anti-caspase 3 antibodies were used for apoptosis investigation. The quantities of p53, caspase 3, and NOXA were measured by Western blotting for to investigate the apoptosis pathway. Results: Cellular toxicity on the human lens epithelium markedly increased with time after UV exposure. On Hoechst staining, we found that apoptosis also remarkably increased after exposure to ultraviolet light, compared with a control group. In the immunochemical study using anti-caspase 3 antibodies, active caspase 3 significantly increased after exposure to ultraviolet light. On Western blotting, p53 decreased, while caspase 3 and NOXA increased. Conclusions: Exposure of cultured human lens epithelial cell lines to ultraviolet light induces apoptosis, which promotes the expression of NOXA and caspase 3 increases without increasing p53. This may suggest that UV induced apoptosis is caused by a p53-independent pathway in human lens epithelial cells.
( Seong Taeck Kim ),( Ji Woong Chun ),( Geon Park ),( Jae Woong Koh ) 대한안과학회 2014 Korean Journal of Ophthalmology Vol.28 No.4
Purpose: To investigate the relationship between plasma TDRD7 mRNA and lens transparency, and to evaluate plasma TDRD7 mRNA as a potential marker for cataracts and its sub-type by quantitatively analyzing human peripheral blood. Methods: Plasma RNA was extracted from 40 patients with cataracts, and 30 normal controls of matched age and gender. Blood cholesterol and fasting glucose were measured, and the RNA extracted from the sample was synthesized into cDNA. After polymerase chain reaction, the results were compared after quantifying the TDRD7 mRNA using ABL1 mRNA for normalization. We analyzed the relative gene expression data via the ΔΔCt method. Results: The normalized 2.ΔΔCt of plasma TDRD7 mRNA based on ABL1 mRNA was 1.52 ± 0.63 in the case of the control group and 1.05 ± 0.34 in the case of the cataract patients, and the TDRD7 expression level of the cataract patients was lower than that of the control group (p = 0.048). The comparison of the genetic values of different types of cataracts demonstrated that the TDRD7 expression level of the cortical type and mixed type were lower than those of the nuclear type and posterior subcapsular opacity type (p = 0.017). Conclusions: Human cataracts and the TDRD7 gene loss-of-function mutations are strongly causally related, as the expression level of plasma TDRD7 mRNA in patients with cataracts was statistically significantly lower than in the normal control group.
당뇨병 유발 쥐에서 신경 세포 증식에 대한 저선량 방사선의 효과
김두순(Doo Soon Kim),강진오(Jin Oh Kang),홍성언(Seong Eon Hong),김상기(Sang Ki Kim),이택현(Taeck-Hyun Lee),김창주(Chang-Ju Kim) 대한방사선종양학회 2005 Radiation Oncology Journal Vol.23 No.3
목 적: 저선량 방사선이 당뇨쥐의 해마에서 신경 세포의 증식에 미치는 영향을 확인하기 위해서이다. 대상 및 방법: 정상 쥐를 대상으로 방사선을 조사하지 않은 군, 0.1 Gy 방사선 조사군, 10 Gy 방사선 조사군으로 나눠 방사선이 정상 신경 세포의 증식에 미치는 영향을 확인하였다. 당뇨쥐를 대상으로 한 이차 실험은 방사선을 조사하지 않은 정상 쥐, 방사선을 조사하지 않은 당뇨쥐, 0.01 Gy 방사선 조사한 당뇨쥐, 0.1 Gy를 조사한 당뇨쥐,1 Gy를 조사한 당뇨쥐, 10 Gy를 조사한 당뇨쥐로 나눠 시행하였다. 5-bromo-2’-deoxyuridine (BrdU)를 이용한 면역염색을 시행하여 쥐 해마의 치아이랑 세포의 수를 세어 비교하였다.결 과: BrdU 양성 세포의 수는 대상으로 방사선을 조사하지 않은 정상쥐, 0.1 Gy 방사선 조사한 정상쥐, 10 Gy 방사선 조사한 정상쥐에서 각각 45.96±3.42/mm2, 59.34±5.20/mm2, 19.26±2.98/mm2로 나타났다. 당뇨쥐를 대상으로한 이차 실험에서 BrdU 양성 세포의 수는 방사선을 조사하지 않은 정상 쥐, 방사선을 조사하지 않은 당뇨쥐, 0.01 Gy 방사선 조사한 당뇨쥐, 0.1 Gy를 조사한 당뇨쥐, 1 Gy를 조사한 당뇨쥐, 10 Gy를 조사한 당뇨쥐에서 각각 55.44±8.57/mm2, 33.33±6.46/mm2, 67.75±10.54/mm2, 66.63±10.05/mm2, 23.59±6.37/mm2, 14.34±7.22/mm2로 나타났다.결 론: 0.1 Gy 이하의 저선량 방사선은 당뇨쥐의 해마 치아이랑세포의 수를 증가시켜 세포 증식효과를 보이는 것을 확인하였다. Purpose: To investigate the effect of low dose radiation on neuronal cell proliferation in diabetic rats. Materials and Methods: A group of rats (first group) were divided into three subgroups (nondiabetic control, nondiabetic 0.1 Gy and nondiabetic 10 Gy groups) to determine the effect of radiation on normal hippocampal neuronal cell proliferation. A further group of rats (second group) were divided into six subgroups (nondiabetic control, diabetic control, diabetic 0.01 Gy, diabetic 0.1 Gy, diabetic 1 Gy and diabetic 10 Gy groups) to determine the effect of radiation on hippocampal neuronal cell proliferation under diabetic conditions. Using immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU), the number of neuronal cells in the dentate gyrus of all the groups was counted. Results: The number of BrdU-positive cells in the dentate Gyrus of the nondiabetic control, nondiabetic 0.1 Gy and nondiabetic 10 Gy subgroups of the first group were 45.96±3.42, 59.34±5.20 and 19.26±2.98/mm2, respectively. The number of BrdU-positive cells in the dentate gyrus of the diabetic control, diabetic 0.01 Gy, diabetic 0.1 Gy, diabetic 1 Gy and diabetic 10 Gy subgroups of the second group were 55.44±8.57, 33.33± 6.46, 67.75±10.54, 66.63±10.05, 23.59±6.37 and 14.34±7.22/mm2, respectively. Conclusion: Low dose radiation enhances cell proliferation in the dentate gyrus of STZ-induced diabetic rats.
Su Jeong Song,Kyung Seek Choi,Jong Chul Han,Donghyun Jee,Jin Wook Jeoung,Young Joon Jo,Jae Yong Kim,Ko Eun Kim,Seong Taeck Kim,Ji Woong Lee,Tae Eun Lee,Dong Hui Lim,Chan Yun Kim,Hyun Woong Kim,Sang Wo 대한안과학회 2021 Korean Journal of Ophthalmology Vol.35 No.4
This report provides a detailed description of the methodology for ophthalmic examinations according to the Korea National Health and Nutrition Examination Survey (KNHANES) VII and VIII (from 2017 to 2021). The KNHANES is a nationwide survey which has been performed since 1998 in representatives of whole Korean population. During the KNHANES VII and VIII, in addition to the ophthalmic questionnaire, intraocular pressure measurement, visual field test, auto refractometry, axial length and optical coherence tomography measurements were included. This new survey will provide not only provide normative and pathologic ophthalmic data including intraocular pressure, refractive error, axial length, visual field and precise measurement of anterior segment, macula and optic nerve with optical coherence tomography, but also a more accurate diagnosis for major adult blindness diseases, including age-related macular degeneration, diabetic retinopathy, glaucoma, and other ocular diseases, for the national Korean population.
김성택,김대현,Seong Taeck Kim,Dae Hyun Kim 대한안과학회 2009 대한안과학회지 Vol.50 No.4
Purpose: To report a case of an intraorbital foreign body after gunshot wounds. We describe this injury and report the treatment outcomes. Case summary: A patient who had been previously shot in his left zygomatic bone by an airgun visited our hospital complaining of visual disturbance in February of 2007. No external injuries on any part of the patients’ body or abnormal neurological signs were observed. Ophthalmic examinations including a visual acuity test, slit lamp examination, pupillary light reflex test, fundus examination, skull X-ray, and computed tomography (CT) were performed. Upon initial examination, his visual acuity was hand motion. The pupillary light reflex test revealed an afferent pupillary defect in the left eye. Additionally, chemosis and eyelid edema were observed; however, no abnormal findings for the retina were observed in the fundus examination. Orbital computed tomography revealed metallic foreign bodies in the intraorbital retrobulbar space and partial injury of optic nerve was observed. Rather than surgical foreign body removal, antibiotic treatment was enforced with no complications other than approximately 12 prism diopters of exodeviation of the left eye in a Krimsky prism test after the course of a year. As for the patient’s vision, the visual acuity of the left eye remained the same as before treatment, but revealed an improvement of 0.1 in left gaze. Conclusions: We report a case of a gunshot injury in the intraorbital retrobulbar space without aggravation of visual function and complication despite the foreign bodies not being removed. J Korean Ophthalmol Soc 2009;50(4):649-655
슬라이딩 모드 제어기를 이용한 EV의 차량 간격 자동 제어
임희성(Hui-Seong Lim),신수철(Soo-Cheol Shin),박상훈(Sang-Hoon Park),이택기(Taeck-Kie Lee),김영렬(Young-Real Kim),원충연(Chung-Yuen Won) 전력전자학회 2010 전력전자학술대회 논문집 Vol.2010 No.11
In this paper, the ACC(Adaptive Cruise Control) method of Electric Vehicle using sliding mode controller is proposed. The IPMSM is safely controlled as safe distance using distance sensor at front of vehicle. The speed of EV is controlled to ensure safe distance using sliding mode controller. The sliding mode controller is suitable to apply nonlinear system like EV. In this paper, IPMSM speed control ability is verified by simulation using PSIM.
Kang, Jin Oh,Kim, Sang-Ki,Hong, Seong-Eon,Lee, Taeck-Hyun,Kim, Chang-Ju The Korean Academy of Medical Sciences 2006 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.21 No.3
<P>We investigated the effect of low dose radiation on diabetes induced suppression of neurogenesis in the hippocampal dentate gyrus of rat. After 0.01 Gy, 0.1 Gy, 1 Gy and 10 Gy radiation was delivered, the dentate gyrus of hippocampus of streptozotocin (STZ)-induced diabetic rats were evaluated using immunohistochemistry for 5-bromo-2-deoxyuridine (BrdU), caspase-3, and terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL) staining. The number of BrdU positive cells in the non-diabetic rats, diabetic rats without radiation, diabetic rats with 0.01 Gy radiation, diabetic rats with 0.1 Gy radiation, diabetic rats with 1 Gy radiation and diabetic rats with 10 Gy radiation were 55.4±8.5/mm<SUP>2</SUP>, 33.3±6.4/mm<SUP>2</SUP>, 67.7±10.5/mm<SUP>2</SUP>, 66.6±10.0/mm<SUP>2</SUP>, 23.5±6.3/mm<SUP>2</SUP>and 14.3±7.2/mm<SUP>2</SUP>, respectively. The number of caspase-3 positive cells was 132.6±37.4/mm<SUP>2</SUP>, 378.6±99.1/mm<SUP>2</SUP>, 15.0±2.8/mm<SUP>2</SUP>, 57.1±16.9/mm<SUP>2</SUP>, 191.8±44.8/mm<SUP>2</SUP>and 450.4±58.3/mm<SUP>2</SUP>, respectively. The number of TUNEL-positive cells was 24.5±2.0/mm<SUP>2</SUP>, 21.7±4.0/mm<SUP>2</SUP>, 20.4±2.0/mm<SUP>2</SUP>, 18.96±2.1/mm<SUP>2</SUP>, 58.3±7.9/mm<SUP>2</SUP>, and 106.0±9.8/mm<SUP>2</SUP>, respectively. These results suggest low doses of radiation paradoxically improved diabetes induced neuronal cell suppression in the hippocampal dentate gyrus of rat.</P>