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RISS 인기검색어
- 검색키워드
- 저자 : Ren-He Xu, Hsiang-Fu Kung, Yong-Chul Bae, 박매자(Mae-Ja Park) (검색결과 6 건)
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Lateral Bearing Capacity and Stiffness Calculation Method of SRC-RC Columns
He Zhang,Pingzhou Cao,Kai Wu,Chao Xu,Lijian Ren 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.5
An SRC-RC column is a vertical hybrid of a steel reinforced concrete (SRC) column and a reinforced concrete (RC) column. To propose the capacity and stiffness calculation method of SRC-RC columns, 768 column models have been simulated using fiber FEM (finite element method) with lateral static loading. These models have different parameters, including: Concrete strength, steel strength, steel sections, steel length and column concrete ratio. Based on simulation results and using a linear regression method, the equations of the secant stiffness and bearing capacity of the SRC-RC hybrid columns are calculated, proposed and verified using the residual analyzing method, ensuring that these calculate equations are accurate.
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Elimination of moving vehicles effects on modal identification of beam type bridges
Wen-Yu He,Xu-Cong Ding,Wei-Xin Ren,Yue-Ling Jing 국제구조공학회 2021 Smart Structures and Systems, An International Jou Vol.28 No.3
The modal parameters identified under operation conditions are normally employed for bridge damage detection. However, the moving vehicles are usually deemed as part of the operation conditions without considering their mass property. Thus, the identified modal parameters belong to the vehicle-bridge system rather than the bridge itself, which would affect the effectiveness of subsequent damage detection. In this paper, the effects of moving vehicles on the identified frequencies and mode shapes under operation conditions are investigated via finite element model. The necessary of considering the moving vehicle effects is demonstrated by comparing the modal parameters variations induced by the moving vehicle and bridge damage. Then the empirical formulas to eliminate the moving vehicle effects considering the vehicle mass, velocity, bridge span and relative position are established by using the orthogonal test and least square method. Finally, examples are conducted to verify of the effectiveness of the proposed empirical formulas.
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Zhao, Xueyan,Yang, Qiang,Zhao, Kewei,Jiang, Chao,Ren, Dongren,Xu, Pan,He, Xiaofang,Liao, Rongrong,Jiang, Kai,Ma, Junwu,Xiao, Shijun,Ren, Jun,Xing, Yuyun Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.7
In the last few decades, transgenic animal technology has witnessed an increasingly wide application in animal breeding. Reproductive traits are economically important to the pig industry. It has been shown that the bone morphogenetic protein receptor type IB (BMPR1B) A746G polymorphism is responsible for the fertility in sheep. However, this causal mutation exits exclusively in sheep and goat. In this study, we attempted to create transgenic pigs by introducing this mutation with the aim to improve reproductive traits in pigs. We successfully constructed a vector containing porcine BMPR1B coding sequence (CDS) with the mutant G allele of A746G mutation. In total, we obtained 24 cloned male piglets using handmade cloning (HMC) technique, and 12 individuals survived till maturation. A set of polymerase chain reactions indicated that 11 of 12 matured boars were transgene-positive individuals, and that the transgenic vector was most likely disrupted during cloning. Of 11 positive pigs, one (No. 11) lost a part of the terminator region but had the intact promoter and the CDS regions. cDNA sequencing showed that the introduced allele (746G) was expressed in multiple tissues of transgene-positive offspring of No.11. Western blot analysis revealed that BMPR1B protein expression in multiple tissues of transgene-positive $F_1$ piglets was 0.5 to 2-fold higher than that in the transgene-negative siblings. The No. 11 boar showed normal litter size performance as normal pigs from the same breed. Transgene-positive $F_1$ boars produced by No. 11 had higher semen volume, sperm concentration and total sperm per ejaculate than the negative siblings, although the differences did not reached statistical significance. Transgene-positive $F_1$ sows had similar litter size performance to the negative siblings, and more data are needed to adequately assess the litter size performance. In conclusion, we obtained 24 cloned transgenic pigs with the modified porcine BMPR1B CDS using HMC. cDNA sequencing and western blot indicated that the exogenous BMPR1B CDS was successfully expressed in host pigs. The transgenic pigs showed normal litter size performance. However, no significant differences in litter size were found between transgene-positive and negative sows. Our study provides new insight into producing cloned transgenic livestock related to reproductive traits.
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김재봉(Jae-bong Kim),이효상(Hyo-sang Lee),노동현(Dong-Hyun Roh),황유석(Yoo-Seok Hwang),Ren-He Xu, Hsiang-Fu Kung, Yong-Chul Bae, 박매자(Mae-Ja Park) 대한해부학회 2000 Anatomy & Cell Biology Vol.33 No.5
BMP-4 signaling is mediated through Smad proteins which may translocate to the nucleus to activate transcription. Little is known about how BMP-4 signaling regulates the transcription of its target genes, e.g., Xvent genes. Therefore, we isolated the genomic clone of a BMP-4 responsive homeobox gene, Xbr-1a/Xvent-2. This clone contains a promoter and three exons for the entire coding region. Using the primer extension, we identified the transcription initiation site corresponding to position -64 bp upstream to the ATG codon of the Xvent-2 gene. The promoter was linked to the luciferase reporter gene, and promoter activity determined by luciferase assay. The temporal promoter activity peaked between embryonic stages 13~17, in agreement with its temporal mRNA expression in the whole embryo. Through the serial deletion mutation, the upstream -235 bp of the promoter retains the full transcriptional activity, and is regulated by BMP-4 signaling. The present results suggest that the BMP-4 responsive element is located on the upstream 235 bp of the promoter. BMP-4의 세포 내 신호전달은, Smad 단백질이 세포핵내로 이동하여 표적 유전자의 전사를 자극하는 과정에 의하여, 매개되는 것으로 알려져 있지만, BMP-4가 자신의 표적유전자 중의 하나인, Xvent유전자의 전사를 어떻게 조절하는지는 잘 알려져 있지 않다. 본 실험실에서는 BMP-4 responsive homeobox 유전자인, Xbr-1a/Xvent-2의 genomic clone을 분리하였으며, 이렇게 분리된 clone에는 promoter와 coding region의 전부위에 해당하는 세개의 exon이 들어 있다는 것을 알 수 있었다. 또한 primer extension 실험결과, 전사시작 지점이 Xvent-2 유전자의 ATG 코돈에서 상류쪽 -64 bp이란 것을 알 수 있었다. Promoter 부위를 luciferase reporter 유전자에 연결한 다음, promoter 활성을 luciferase assay를 통하여 측정하였다. Promoter 활성은, 개구리 배자에서의 Xvent-2 유전자의 mRNA 발현양상과 비슷하게, 13~17 배자기에 최고치를 나타내었으며, serial deletion mutation실험을 통하여, promoter 부위의 -235 bp 부위에서 BMP-4/Smad1에 의하여 조절되는, 완전한 전사활성이 관찰되었다. 본 실험의 결과는, Xbr-1a/Xvent-2유전자의 promoter부위에서, 상류쪽 -235 bp 부위에 BMP-4 response element가 존재한 다는 것을 보여주는 것으로 사료된다.
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