Posttranscriptional deregulation of Src due to aberrant miR34a and miR203 contributes to gastric cancer development

Qiang Hao,Xiao Zhao Lu,Nan Nan Liu,Xiao Chang Xue,Meng Li,Cun Zhang,Xin Qin,Wei Na Li,Zhen Shu,Bin Song,Qing Wang,Li Qiang Song,Wei Zhang,Ying Qi Zhang 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.6

A Rapid Life Cycle Assessment Method based on Green Features in Supporting Conceptual Design

Qiang Meng,Fang-yi Li,Li-rong Zhou,Jing Li,Qin-qin Ji,Xiaodong Yang 한국정밀공학회 2015 International Journal of Precision Engineering and Vol.2 No.2

A Rapid Life Cycle Assessment (RLCA) method based on Green features is proposed in order to solve the inherent limitations of conventional Life Cycle Assessment (LCA), such as long period, massive data requirement, which result in difficulties in supporting product conceptual design. Firstly, Green Feature is proposed in supporting the LCA in conceptual design, where a mapping relationship is established between green feature and design information to achieve the transformation from the design information to green features. Secondly, product conceptual design model is proposed based on modular configuration. The approximate products program will be obtained through retrieval and matching of each module of product using the knowledge base and case base. Thirdly, Intuitionistic Fuzzy theory and Monte Carlo method are respectively used to process the qualitative and quantitative uncertain information of green features in order to ensure the accuracy of the evaluation results. Besides, the appropriate life cycle impact assessment method is selected to complete the life cycle impact assessment and obtain the LCA results. Consequently, the RLCA of product design program is completed to support product green design. Finally, a fan of a ventilation system is studied as an example to verify the proposed RLCA theory.

Reversible Sub-Feature Retrieval: Toward Robust Coverless Image Steganography for Geometric Attacks Resistance

( Qiang Liu ),( Xuyu Xiang ),( Jiaohua Qin ),( Yun Tan ),( Qin Zhang ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.3

Traditional image steganography hides secret information by embedding, which inevitably leaves modification traces and is easy to be detected by steganography analysis tools. Since coverless steganography can effectively resist steganalysis, it has become a hotspot in information hiding research recently. Most coverless image steganography (CIS) methods are based on mapping rules, which not only exposes the vulnerability to geometric attacks, but also are less secure due to the revelation of mapping rules. To address the above issues, we introduced camouflage images for steganography instead of directly sending stego-image, which further improves the security performance and information hiding ability of steganography scheme. In particular, based on the different sub-features of stego-image and potential camouflage images, we try to find a larger similarity between them so as to achieve the reversible steganography. Specifically, based on the existing CIS mapping algorithm, we first can establish the correlation between stego-image and secret information and then transmit the camouflage images, which are obtained by reversible sub-feature retrieval algorithm. The received camouflage image can be used to reverse retrieve the stego-image in a public image database. Finally, we can use the same mapping rules to restore secret information. Extensive experimental results demonstrate the better robustness and security of the proposed approach in comparison to state-of-art CIS methods, especially in the robustness of geometric attacks.

Combined Administration of the Mixture of Honokiol and Magnolol and Ginger Oil Evokes Antidepressant-like Synergism in Rats

Li-Qin Qiang,Ling-Dong Kong,Cai-Ping Wang,Fu-Meng Wang,Ying Pan,Li-Tao Yi,Xian Zhang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.9

Magnolia bark combined with ginger rhizome is a common drug pair in traditional Chinese prescriptions for the treatment of depression. In the present study, we examined antidepressant-like effects of the mixture of honokiol and magnolol (HMM) from magnolia bark and essential oil from ginger rhizome (OGR) alone and in combination in chronic unpredictable mild stress (CUMS) of rats. Behavioral (sucrose intake, immobility time of forced swimming test) and biochemical parameters [serotonin (5-HT) in prefrontal cortex, hippocampus, and striatum, gastric mucosa cholecystokinin (CCK) and serum gastrin (GAS) levels] were simultaneously examined in the CUMS rats. 20 mg/kg HMM alone, but not OGR, significantly increased sucrose intake and reduced immobility time in the CUMS rats. Moreover, 20 mg/kg HMM and 14 mg/kg OGR in combination exhibited significant synergistic effects on sucrose intake increase and immobility time reduction in the CUMS rats. HMM elevated 5-HT levels in various brain regions, and OGR reduced gastric mucosa CCK and serum GAS levels in the CUMS rats. These results suggested that the synergistic antidepressant-like effects of compatibility of HMM with OGR might be mediated simultaneously by regulation of the serotonergic and gastroenteric system functions. These findings also provided a pharmacological basis for the clinical application of this drug pair of magnolia bark and ginger rhizome in traditional Chinese medicine.

MicroRNA-214-mediated UBC9 expression in glioma

( Zhi Qiang Zhao ),( Xiao Chao Tan ),( Ani Zhao ),( Li Yuan Zhu ),( Bin Yin ),( Jiang Ang Yuan ),( Bo Qin Qiang ),( Xiao Zhong Peng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.11

It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lines compared to a normal control. UBC9 knockdown by small interfering RNA (siRNA) affected cell proliferation and apoptosis in T98G cells. Further experiments revealed that microRNA (miR)-214 directly targeted the 3` untranslated region (UTR) of UBC9 and that there was an inverse relationship between the expression levels of miR-214 and UBC9 protein in glioma tissues and cells. MiR-214 overexpression suppressed the endogenous UBC9 protein and affected T98G cell proliferation. These findings suggest that miR-214 reduction facilitates UBC9 expression and is involved in the regulation of glioma cell proliferation.

Isolation and phylogenetic analysis of hemagglutinin gene of H9N2 influenza viruses from chickens in South China from 2012 to 2013

Han-Qin Shen,Zhuan-Qiang Yan,Fan-Gui Zeng,Chang-Tao Liao,Qing-Feng Zhou,Jian-Ping Qin,Qingmei Xie,Yingzuo Bi,Feng Chen 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

As part of our ongoing influenza surveillance program in South China, 19 field strains of H9N2 subtype avian influenza viruses (AIVs) wereisolated from dead or diseased chicken flocks in Guangdong province, South China, between 2012 and 2013. Hemagglutinin (HA) genes ofthese strains were sequenced and analyzed and phylogenic analysis showed that 12 of the 19 isolates belonged to the lineage h9.4.2.5, whilethe other seven belonged to h9.4.2.6. Specifically, we found that all of the viruses isolated in 2013 belonged to lineage h9.4.2.5. The lineageh9.4.2.5 viruses contained a PSRSSR↓GLF motif at HA cleavage site, while the lineage h9.4.2.6 viruses contained a PARSSR↓GLF at thesame position. Most of the isolates in lineage h9.4.2.5 lost one potential glycosylation site at residues 200–202, and had an additional oneat residues 295–297 in HA1. Notably, 19 isolates had an amino acid exchange (Q226L) in the receptor binding site, which indicated that theviruses had potential affinity of binding to human like receptor. The present study shows the importance of continuing surveillance of newH9N2 strains to better prepare for the next epidemic or pandemic outbreak of H9N2 AIV infections in chicken flocks.

An Efficient Blind Detection Algorithm of Median Filtered Image

Yongzhen Ke,Fan Qin,Weidong Min,Qiang Zhang 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.1

Due to the significant advances of digital photography and the availability of many powerful photo editing tools, it becomes easier to create forgery images by non-professional users. Median filtering that is usually applied to erase the forensically significant fingerprints has recently received increased attention. In this paper, we present an effective blind forensic algorithm to detect the median filtering manipulation. First, the median filtered residual (MFR) is generated by computing the difference between a testing image and a median filtered version of itself. Then, three feature sets including histogram, autocorrelation and gradient are extracted from the median filter residual. Last, those features are fetched into support vector machine (SVM) for training and classification. Our experimental results demonstrate that our proposed forensic method achieves not only better detection rate but also lower computational complexity compared with other existing median filtering detection methods. Our proposed forensic method also can locate local median filtering of image effectively.

Expression of the Arabidopsis vacuolar $H^+$-pyrophosphatase gene AVP1 in peanut to improve drought and salt tolerance

Qin, Hua,Gu, Qiang,Kuppu, Sundaram,Sun, Li,Zhu, Xunlu,Mishra, Neelam,Hu, Rongbin,Shen, Guoxin,Zhang, Junling,Zhang, Yizheng,Zhu, Longfu,Zhang, Xianlong,Burow, Mark,Payton, Paxton,Zhang, Hong 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

The Arabidopsis gene AVP1 encodes an $H^+$-pyrophosphatase that functions as a proton pump at the vacuolar membranes, generating a proton gradient across vacuolar membranes, which serves as the driving force for many secondary transporters on vacuolar membranes such as $Na^+/H^+$-antiporters. Overexpression of AVP1 could improve drought tolerance and salt tolerance in transgenic plants, suggesting a possible way in improving drought and salt tolerance in crops. The AVP1 was therefore introduced into peanut by Agrobacterium-mediated transformation. Analysis of AVP1-expressing peanut indicated that AVP1-overexpression in peanut could improve both drought and salt tolerance in greenhouse and growth chamber conditions, as AVP1-overexpressing peanuts produced more biomass and maintained higher photosynthetic rates under both drought and salt conditions. In the field, AVP1-overexpressing peanuts also outperformed wild-type plants by having higher photosynthetic rates and producing higher yields under low irrigation conditions.

Expression of Cdc25B mRNA in Duodenal Mucosa of Chicken

Qin, Junhui,Zhang, Hui,Bao, Huijun,Zhou, Qiang,Liu, Yi,Xu, Chunsheng,Chu, Xiaohong,Chen, Qiusheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4

Cdc25B is a mitotic regulator that might act as a starter phosphatase to initiate the positive feedback loop at the entry into mitotic (M) phase. In the present study, distribution of Cdc25B mRNA in duodenal mucosa of the chicken was demonstrated by means of in situ hybridization histochemistry (ISHH) using sense and antisense digoxigenin (DIG)-labeled RNA probes. The results showed that there were many labeled cells distributing in the duodenal mucosa of the adult chicken. Of these labeled cells, 81.60${\pm}$9.63% of Cdc25B mRNA positive cells was distributed in the basilar part and mid-portion of the intestinal gland and 36.21${\pm}$8.81% in the middle and basilar portion of villi of the small intestine of the chicken, respectively. Most of these labeled cells were positive in the regions of the stem cell and proliferation. The signals of ISHH decreased from basilar to upper part in the crypt of Lieberkuhn and weakened in the inferior villi of the duodenum. Moreover, the positive signals were both in the cytoplasm and cell nucleus. However, the labeled cells were negative in both the lamina muscularis mucosae and muscular layer. The results of ISHH suggested the existence of Cdc25B mRNA and vigorous proliferation activities in the duodenal mucosa of adult chicken, replenishing the cells which had sloughed off from the superior part of the villus. Our results provide some molecular evidence for a regular pattern of avian intestinal epitheliosis and functional partition and provide an approach to further study of the locations of Cdc25B in the chicken.

Morphological and Hemodynamic Parameters for Middle Cerebral Artery Bifurcation Aneurysm Rupture Risk Assessment

Qin, Hao,Yang, Qixia,Zhuang, Qiang,Long, Jianwu,Yang, Fan,Zhang, Hongqi The Korean Neurosurgical Society 2017 Journal of Korean neurosurgical society Vol.60 No.5

Objective : To investigate the morphological and hemodynamic parameters associated with middle cerebral artery (MCA) bifurcation aneurysm rupture. Methods : A retrospective study of 67 consecutive patients was carried out based on 3D digital subtraction angiography data. Morphological and hemodynamic parameters including aneurysm size parameters (dome width, height, and perpendicular height), longest dimension from the aneurysm neck to the dome tip, neck width, aneurysm area, aspect ratio, Longest dimension from the aneurysm neck to the dome tip (Dmax) to dome width, and height-width, Bottleneck factor, as well as wall shear stress (WSS), low WSS area (LSA), percentage of LSA (LSA%) and energy loss (EL) were estimated. Parameters between ruptured and un-ruptured groups were analyzed. Receiver operating characteristics were generated to check prediction performance of all significant variables. Results : Sixty-seven patients with MCA bifurcation aneurysm were included (31 unruptured, 36 ruptured). Dmax (p=0.008) was greater in ruptured group than that in un-ruptured group. D/W (p<0.001) and the percentage of the low WSS area ($0.09{\pm}0.13$ vs. $0.01{\pm}0.03$, p<0.001) were also greater in the ruptured group. Moreover, the EL in ruptured group was higher than that in unruptured group ($6.39{\pm}5.04$ vs. $1.53{\pm}0.86$, p<0.001). Multivariate regression analysis suggested D/W and EL were significant predictors of rupture of MCA bifurcation aneurysms. Correlation analyses revealed the D/W value was positively associated with the EL (R=0.442, p<0.01). Conclusion : D/W and EL might be the most two favorable factors to predict rupture risk of MCA bifurcation aneurysms.