A Qualitative Case Study on Sibling Relationship Experiences of Adolescents Discharged from Child Care Institutions

Shinae Park,Sujin Kang,Okchai Choi 사단법인 인문사회과학기술융합학회 2019 예술인문사회융합멀티미디어논문지 Vol.9 No.1

This study investigated the sibling relationships in adolescents discharged from child care institutions. The purpose of this study was to influence the development of social welfare intervention plans to support sibling relationships in brothers and/or sisters who grow up in child care institution. We enrolled four adolescents who had grown up together with their sibling(s) at the same child care institution. We examined the individual responses of the adolescents after in-depth interviews, which were conducted between March and August 2017. Our qualitative case study revealed that the adolescents had not been exposed to sufficient interactions or opportunities at the child care institutions that enabled them to form strong sibling relationships with each their sibling(s). After being discharged, adolescents remained on awkward terms with their sibling(s) onto adolescence, but did not sever family ties. Based on these findings, the social welfare interventions suggested in this study aim to promote and support positive sibling relationships among siblings discharged from the same child care institutions.

β-Cell–Derived Angiopoietin-1 Regulates Insulin Secretion and Glucose Homeostasis by Stabilizing the Islet Microenvironment

Park, Ho Seon,Kim, Hak Zoo,Park, Jong Suk,Lee, Junyeop,Lee, Seung-Pyo,Kim, Hail,Ahn, Chul Woo,Nakaoka, Yoshikazu,Koh, Gou Young,Kang, Shinae American Diabetes Association 2019 Diabetes Vol. No.

<P>Islets are highly vascularized for prompt insulin secretion. Although angiopoietin-1 (Ang1) is a well-known angiogenic factor, its role in glucose homeostasis remains largely unknown. The objective of this study was to investigate whether and how Ang1 contributes to glucose homeostasis in response to metabolic challenge. We used inducible systemic Ang1 knockout (Ang1<SUP>sys−/−</SUP>) and β-cell–specific Ang1 knockout (Ang1<SUP>β-cell−/−</SUP>) mice fed a high-fat diet for 24 weeks. Although the degree of insulin sensitivity did not differ between Ang1<SUP>sys−/−</SUP> and Ang1<SUP>sys+/+</SUP> mice, serum insulin levels were lower in Ang1<SUP>sys−/−</SUP> mice, resulting in significant glucose intolerance. Similar results were observed in Ang1<SUP>β-cell−/−</SUP> mice, suggesting a critical role of β-cell–derived Ang1 in glucose homeostasis. There were no differences in β-cell area or vasculature density, but glucose-stimulated insulin secretion was significantly decreased, and PDX-1 expression and GLUT2 localization were altered in Ang1<SUP>β-cell−/−</SUP> compared with Ang1<SUP>β-cell+/+</SUP> mice. These effects were associated with less pericyte coverage, disorganized endothelial cell ultrastructure, and enhanced infiltration of inflammatory cells and upregulation of adhesion molecules in the islets of Ang1<SUP>β-cell−/−</SUP> mice. In conclusion, β-cell–derived Ang1 regulates insulin secretion and glucose homeostasis by stabilizing the blood vessels in the islet and may be a novel therapeutic target for diabetes treatment in the future.</P>

모둠 독서 토론 담화에 드러난 상호작용 양상 - 모둠원의 관계와 위치 설정을 중심으로

박신애 ( Park Shinae ) 고려대학교 한국어문교육연구소 2018 한국어문교육 Vol.26 No.-

이 연구는 중학교 수업 시간에 실시한 모둠 독서 토론 담화를 비판적 담화 분석의 관점에서 분석하여 모둠 구성원의 관계가 독서 토론 활동에 어떠한 영향을 미치는지를 살펴보았다. 학생들은 독서 토론 과정에서 발화 빈도나 주요 발화 양상에서 차이를 보였다. 이는 담화에 드러난 대명사와 조사의 사용, 부정적 표현과 함께 모둠 내에서 일종의 권력으로 작용하여, 모둠원들이 암묵적으로 각자의 위치를 리더, 리더와 대립, 방관, 소외로 위치 짓는 데에 영향을 끼쳤다. 또한 모둠 내에서 권력을 가진 구성원이 지속적으로 부정적인 감정을 표현하였을 때 모둠 전체에 부적 정서가 전이되어 독서 토론 활동에도 지장을 준다는 점을 확인하였다. 본 연구는 소수를 대상으로 한 사례연구라는 점에서 일반화에 한계가 있으나, 학생들의 모둠 독서 토론 과정에 주목하여 또래 간 상호작용 양상을 실증적으로 살핀 것에 의의가 있다. This study examined the influence that the relationships among group members had on reading discussion activities by analyzing the group discussion talks in class, from the perspective of critical discourse analysis. Students showed differences in the frequency of utterances and patterns of major utterances during the reading discussion. Along with the use of pronouns, postposition, and negative expressions, revealed during the discourse, this acted as a sort of power within the group, which tacitly showed positions of each one: leader, conflicted-leader, onlooker, and outsider. In addition, we could confirm that when members with power in the team continuously express negative emotions, these negative emotions spread to the whole group, which eventually hinders reading discussion activities. This study is limited to generalize the above findings, as it is a case study done with a small number of students. Nevertheless, it is meaningful in that it examined the interaction patterns of peers, paying close attention the process of students' group reading discussion.

청소년의 긍정적·부정적 독서 경험에 관한 고찰 ― 독서 경험의 계기와 영향 요인을 중심으로

박신애 ( Park Shinae ) 국어교육학회 2022 國語敎育學硏究 Vol.57 No.2

본 연구는 청소년에게 긍정적 또는 부정적인 영향을 준 독서 경험이 무엇이며 어떠한 특성이 있는지에 관하여 고찰하였다. 이를 위해, 청소년 1,000명을 대상으로 설문조사한 긍정적, 부정적 독서 경험에 관한 서술형 응답을 분석하였다. 그 결과 청소년의 긍정적, 부정적 독서 경험의 계기를 ‘외적 계기(가정, 학교, 사회, 텍스트)’와 ‘내적 계기’로 분류하였으며, 성별, 학교급별, 독자 유형별로 다소 차이가 있음을 밝혔다. 설문의 구체적인 내용을 분석하여, 비슷한 경험을 했다 하더라도 ‘선택권과 자율성’, ‘새로운 경험이나 감정’, ‘다른 사람과의 소통’ 유무에 의해 독서 경험을 긍정적, 부정적으로 상반되게 인식함을 확인하였다. This study examined the positive and negative effects of reading experiences on adolescents and characteristics of each reading experience. For this purpose, we analyzed descriptive responses regarding positive and negative reading experiences collected via a survey of 1,000 adolescents. Based on the results, the factors of positive and negative reading experiences of adolescents were classified into an external factor(home, school, society, text) and internal factors of individual. It was found that the reasons for the reading experience differed by gender, school level, and reader type. The specific contents of the responses were then analyzed in detail confirming that even if adolescents had similar experiences, they perceived the reading experience positively and negatively differently depending on the presence or absence of “choice and autonomy”, “new experience or emotion”, and “communication with others”.

노화를 고려한 배터리의 내부 단락 진단 및 용량 추정 알고리즘

박신아(ShinA Park),박민준(Minjun Park),서민환(Minhwan Seo),송영빈(Youngbin Song),김상우(Sang Woo Kim) 대한전기학회 2020 정보 및 제어 심포지엄 논문집 Vol.2020 No.10

Endothelial Progenitor Cell Cotransplantation Enhances Islet Engraftment by Rapid Revascularization

Kang, Shinae,Park, Ho Seon,Jo, Anna,Hong, Shin Hee,Lee, Han Na,Lee, Yeon Yi,Park, Joong Shin,Jung, Hye Seung,Chung, Sung Soo,Park, Kyong Soo American Diabetes Association 2012 Diabetes Vol.61 No.4

<P><B/></P><P>Impaired revascularization of transplanted islets is a critical problem that leads to progressive islet loss. Since endothelial progenitor cells (EPCs) are known to aid neovascularization, we aimed to enhance islet engraftment by cotransplanting EPCs with islets. Porcine islets, with (islet-EPC group) or without (islet-only group) human cord blood–derived EPCs, were transplanted into diabetic nude mice. The islet-EPC group reached euglycemia by ∼11 days posttransplantation, whereas the islet-only group did not. Also, the islet-EPC group had a higher serum porcine insulin level than the islet-only group. Islets from the islet-EPC group were more rapidly revascularized at the early period of transplantation without increment of final capillary density at the fully revascularized graft. Enhanced revascularization rate in the islet-EPC group was mainly attributed to stimulating vascular endothelial growth factor-A production from the graft. The rapid revascularization by EPC cotransplantation led to better graft perfusion and recovery from hypoxia. EPC cotransplantation was also associated with greater β-cell proliferation, probably by more basement membrane production and hepatocyte growth factor secretion. In conclusion, cotransplantation of EPCs and islets induces better islet engraftment by enhancing the rate of graft revascularization. These findings might provide a directly applicable tool to enhance the efficacy of islet transplantation in clinical practice.</P>

Unusual truncation of <i>N</i>-acylated peptoids under acidic conditions

Kim, Soomin,Biswas, Goutam,Park, Shinae,Kim, Arim,Park, Hyunjung,Park, Eunsook,Kim, Jeongmi,Kwon, Yong-Uk The Royal Society of Chemistry 2014 Organic & biomolecular chemistry Vol.12 No.28

<P>The terminal amino groups of peptoids have often been protected with acetyl groups to improve cell permeability and therapeutic potential, and to prevent the poisoning of the catalysts in organometallic reactions. Interestingly, the unusual truncation of the terminal peptoid unit has sometimes been encountered when the acetylated linear peptoids were treated with a TFA cleavage cocktail. In this study, we systematically investigated the electronic effects of acyl groups on the truncation of <I>N</I>-acylated peptoids to rationalize the formation of the deleted peptoids and to establish an appropriate strategy for preventing such undesired truncation.</P> <P>Graphic Abstract</P><P>Systematic studies on the unusual truncation of <I>N</I>-acylated peptoids were carried out to examine the electronic effects of acyl groups, and thus to control the formation of deletion sequences based on a plausible mechanism. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3ob42572j'> </P>

Bransfield 해협 내 Deception Island 화산암의 멜트포유물 연구: 주원소, 미량원소 그리고 납 동위원소 지구화학

양윤석(Yun-Seok Yang),서정훈(Jung Hun Seo),박숭현(Sung-Hyun Park),박창근(Changkun Park),한승희(Seunghee Han),박계헌(Kye-Hun Park),이신애(Shinae Lee) 대한지질학회 2019 대한지질학회 학술대회 Vol.2019 No.10

Control of Adipogenesis by the SUMO-Specific Protease SENP2

Chung, Sung Soo,Ahn, Byung Yong,Kim, Min,Choi, Hye Hun,Park, Ho Seon,Kang, Shinae,Park, Sang Gyu,Kim, Young-Bum,Cho, Young Min,Lee, Hong Kyu,Chung, Chin Ha,Park, Kyong Soo American Society for Microbiology 2010 Molecular and cellular biology Vol.30 No.9

<B>ABSTRACT</B><P>Here, we demonstrate that SENP2, a desumoylating enzyme, plays a critical role in the control of adipogenesis. SENP2 expression was markedly increased upon the induction of adipocyte differentiation, and this increase was dependent on protein kinase A activation. Remarkably, knockdown of SENP2 led to a dramatic attenuation of adipogenesis with a marked decrease in PPARγ and C/EBPα mRNA levels. Knockdown of SENP2 also caused a marked reduction in the level of C/EBPβ protein but not in that of C/EBPβ mRNA. Interestingly, sumoylation of C/EBPβ dramatically increased its ubiquitination and destabilization, and this increase could be reversed by SENP2. In addition, overexpression of C/EBPβ could overcome the inhibitory effect of SENP2 knockdown on adipogenesis. Furthermore, SENP2 was absolutely required for adipogenesis of preadipocytes implanted into mice. These results establish a critical role for SENP2 in the regulation of adipogenesis by desumoylation and stabilization of C/EBPβ and in turn by promoting the expression of its downstream effectors, such as PPARγ and C/EBPα.</P>

Pre-Transplant Angiotensin II Type 1 Receptor Antibodies and Anti-Endothelial Cell Antibodies Predict Graft Function and Allograft Rejection in a Low-Risk Kidney Transplantation Setting

Shinae Yu,Hee Jae Huh,Kyo Won Lee,Jae Berm Park,Sung Joo Kim,Wooseong Huh,Hye Ryoun Jang,Ghee Young Kwon,Hyung Hwan Moon,Eun-Suk Kang 대한진단검사의학회 2020 Annals of Laboratory Medicine Vol.40 No.5

Background: Non-HLA antibodies, anti-angiotensin II type 1 receptor antibodies (anti-AT1R) and anti-endothelial cell antibodies (AECA), are known to play a role in allograft rejection. We evaluated the role of both antibodies in predicting post-transplant outcomes in low-risk living donor kidney transplantation (LDKT) recipients. Methods: In 94 consecutive LDKT recipients who were ABO compatible and negative for pre-transplant HLA donor-specific antibodies, we determined the levels of anti-AT1Rs using an enzyme-linked immunosorbent assay and the presence of AECAs using a flow cytometric endothelial cell crossmatch (ECXM) assay with pre-transplant sera. Hazard ratio (HR) was calculated to predict post-transplant outcomes. Results: Pre-transplant anti-AT1Rs (≥11.5 U/mL) and AECAs were observed in 36 (38.3%) and 22 recipients (23.4%), respectively; 11 recipients had both. Pre-transplant anti-AT1Rs were a significant risk factor for the development of acute rejection (AR) (HR 2.09; P=0.018), while a positive AECA status was associated with AR or microvascular inflammation only (HR 2.47; P=0.004) throughout the follow-up period. In particular, AECA (+) recipients with ≥11.5 U/mL anti-AT1Rs exhibited a significant effect on creatinine and estimated glomerular filtration rate (P<0.001; P=0.028), although the risk of AR was not significant. Conclusions: Pre-transplant anti-AT1Rs and AECAs have independent negative effects on post-transplant outcomes in low-risk LDKT recipients. Assessment of both antibodies would be helpful in stratifying the pre-transplant immunological risk, even in low-risk LDKT recipients.