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( Sung Woo Moon ),( Ji Ye Jung ),( Young Ae Kang ),( Moo Suk Park ),( Young Sam Kim ),( Se Kyu Kim ),( Joon Chang ),( Hyo Chae Park ),( Chang Young Lee ),( Song Yee Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Primary graft dysfunction (PGD) is a severe type of acute lung injury after lung transplantation and is reported to be associated with early morbidity and mortality.We were to investigate donor, recipient, and perioperative risk factors and outcome of PGD. Methods: We performed a retrospective study using data collected in one tertiary care hospital in South Korea. The patients who underwent lung transplantation between January 2010 and March 2014 were enrolled. The primary outcome was grade 3 PGD (PaO2/ fiO2 < 200 and presence of diffuse infi ltrates on chest radiograph of allograft at 48 or 72 hours after transplant) and PGD grade was defi ned according to International Society for Heart and Lung Transplantation criteria. Results: A total of 61 patients were enrolled and 16 subjects (26.2%) developed grade 3 PGD. In univariate study, higher body mass index (BMI) in recipients, any history of recipient smoking, extracorporeal membrane oxygenation usage before transplantation in recipients, and ischemic time during operation were associated with PGD. Primary recipient diagnosis, transplant type, mean pulmonary artery pressure, donor-smoking history were not related with PGD. In multivariate model, independent risk factors for PGD were BMI in recipients (Odds ratio [OR], 1.290; P=0.048) and total ischemic time during operation (OR, 1.013; P=0.009). PGD was signifi cantly associated with higher re-operation rate (OR, 3.500; P=0.042), longer days of ventilator apply (median 6 days vs.14.5 days; P=0.044), longer intensive care unit stay (median 9 days vs. 17 days; P=0.041) and higher rate of renal replacement therapy (OR, 7.708; P=0.002) after transplantation. Conclusions: We identifi ed risk factors and outcome of grade 3 PGD after lung transplantation. Our fi ndings can be used to develop predictive models for PGD that may allow for modifi cation of risk factors.
( Sung Woo Moon ),( Kyung Soo Chung ),( Ji Ye Jung ),( Young Ae Kang ),( Moo Suk Park ),( Young Sam Kim ),( Joon Chang ),( Se Kyu Kim ),( Hyo Chae Paik ),( Jun Won Cheong ),( Song Yee Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Introduction: Hemophagocytic lymphohistiocytosis (HLH) is a rare but life-threatening complication after solid organ transplantation. HLH has been reported as problem related with kidney and liver transplant, and there are limited reports of HLH after lung transplantation. Case: A 60-year-old man with idiopathic pulmonary fibrosis underwent bilateral lung transplantation. After lung transplantation, acute rejection was suspected and high dose steroid therapy was done. Since postoperative day(POD) 25, thrombocytopenia( platelet 112*1000/uL) and leukopenia(2530/uL)) were presented. The patient complained of intermittent symptom of low-grade fever, chest discomfort and dyspnea. Echocardiography showed stress induced cardiomyopathy and results of peripheral blood smear was nonspeci. c. Pneumonia was developed and patient was treated with antibiotics. Hyperbilirubinemia(total bilirubin 2.1 mg/dL) started to present at POD 50. Results of abdomen sonography was nonspeci. C except for mild splenomegaly (11.4cm). By POD 80, bilirubin was getting higher(total bilirubin 33.3mg/dL, direct bilirubin 25.7mg/dL, gamma glutamyl transpeptidase 260IU/L) and leukopenia and thrombocytopenia was getting aggravated (white blood cell 2080/uL and platelet 57* 1000/uL). The . brinogen was mildly elevated (4210m/dL), triglyceride was normal (127 mg/dL), the ferritin was elevated (4518 ng/mL) and soluble interleukin-2 receptor was elevated (8730U/ml). However, the finding of peripheral blood smear was still nonspeci. c. The cause of pancytopenia, low grade fever and hyperbilirubinemia was unclear, and we conducted bone marrow biopsy on POD 82. The finding showed that histiocytes were frequently seen with occasional hemophagocytes. Taken together, cytopenia, bone marrow hemophagocytes, elevated soluble interleukin-2 receptor and elevated ferritin were positive among laboratory tests listed in diagnostic criteria of HLH. We managed with etoposide and high dose steroid, but patient deteriorated and died on POD 87. Summary: HLH is a significant diagnostic and therapeutic challenge in lung transplantation and is potentially lethal complication. Therefore, clinicians should consider HLH as possible diagnosis in clinical context.
Moon, Ae Ran,Park, Yoonkyung,Chang, Jeong Hwan,Lee, Sang Su Williams & Wilkins Co 2019 Medicine Vol.98 No.10
<P><B>Abstract</B></P><P>We hypothesized that circulating osteoprotegerin (OPG) and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) levels could be associated with vascular calcification, which is predominant in diabetes.</P><P>The study included 71 Korean participants (36 with diabetes and 35 without diabetes), who were sub-grouped according to the results of the ankle–brachial index (ABI) and/or X-ray computed tomography scan (CT scan). Serum OPG and TRAIL levels were assayed using the respective enzyme-linked immunosorbent assay kits. Statistical significance was analyzed using Student's <I>t</I> test between the 2 groups or analysis of variance (ANOVA) among the 4 groups.</P><P>Serum OPG was up-regulated in the participants with diabetes, with peripheral arterial disease (PAD), and/or with vascular calcification. TRAIL down-regulation was more strictly controlled than OPG up-regulation; it was significantly downregulated in the participants with PAD and vascular calcification, but not in the participants with diabetes. Serum OPG and TRAIL were regulated in the participants with femoral, popliteal, and peroneal artery calcification but not in the participants with aortic calcification.</P><P>OPG up-regulation and TRAIL down-regulation were found to be associated with leg lesional vascular calcification; therefore, the average OPG/TRAIL ratio was significantly increased by 3.2-fold in the leg lesional vascular calcification group.</P>
Sang Yoon Kim,Ki-Nam Shim,Joo-Ho Lee,Ji Young Lim,Tae Oh Kim,A. Reum Choe,Chung Hyun Tae,Hye-Kyung Jung,Chang Mo Moon,Seong-Eun Kim,Sung-Ae Jung 대한소화기내시경학회 2019 Clinical Endoscopy Vol.52 No.6
Background/Aims: Endoscopic ultrasonography (EUS) is the most effcient imaging modality for gastric subepithelial tumors (SETs). However, abdominopelvic computed tomography (APCT) has other advantages in evaluating the characteristics, local extension,or invasion of SETs to adjacent organs. This study aimed to compare the diagnostic ability of EUS and APCT based on surgicalhistopathology results. Methods: We retrospectively reviewed data from 53 patients who underwent both EUS and APCT before laparoscopic wedge resectionfor gastric SETs from January 2010 to December 2017 at a single institution. On the basis of histopathology results, we assessed thediagnostic ability of the 2 tests. Results: The overall accuracy of EUS and APCT was 64.2% and 50.9%, respectively. In particular, the accuracy of EUS vs. APCT for thediagnosis of gastrointestinal stromal tumors (GISTs), leiomyomas, and ectopic pancreas was 83.9% vs. 74.2%, 37.5% vs. 0.0%, and 57.1%vs. 14.3%, respectively. Most of the incorrect diagnoses with EUS involved hypoechoic lesions originating in the fourth echolayer, withthe most common misdiagnosed lesions being GISTs mistaken for leiomyomas and vice versa. Conclusions: APCT showed a lower overall accuracy than EUS; however, APCT remains a useful modality for malignant/potentiallymalignant gastric SETs.
Moon, Sung-Hwan,Kang, Sun-Woong,Park, Soon-Jung,Bae, Daekyeong,Kim, Sung Joon,Lee, Hyang-Ae,Kim, Kyung Soo,Hong, Ki-Sung,Kim, Jong Soo,Do, Jeong Tae,Byun, Ki Hyun,Chung, Hyung-Min Elsevier 2013 Biomaterials Vol.34 No.16
<P><B>Abstract</B></P> <P>Embryonic stem cells (ESCs) have the capacity to undergo directed differentiation into contracting cardiomyocytes. Therefore, functional cardiomyocytes derived from human embryonic stem cells (hESC-CMs) are potential candidates for cellular cardiomyoplasty to regenerate the myocardium after infarction. However, the directed differentiation of hESCs induces not only contracting cardiomyocytes but also other cell types. Thus, a risk of teratoma formation and oncologic transformation exists following the transplantation of hESC-CMs containing other cell lineages. In addition, the transplantation of hESC-CMs into the infarcted myocardium limits therapeutic efficacy due to low viability and poor engraftment. In this study, we established an efficient preparation method to obtain pure contracting cardiomyocytes from hESCs. We also developed a delivery system to achieve enhanced viability and a functional connection with the host myocardium after transplantation in a myocardial infarction model. A serum-free medium was used to obtain pure contracting cardiomyocytes from other cell lineages after the cardiac differentiation of hESCs. Aggregates of purified hESC-CMs were formed, and then the expression of cardiomyocyte-specific markers and the viability of the aggregated CMs were examined in hypoxic conditions. In addition, we determined whether the viability of the hESC-CMs and their ability to engraft with the host myocardium could be enhanced by transplanting them as aggregates in a myocardial infarction model. The therapeutic efficacy of the cardiomyocytes was examined by immunohistochemical analyses as well as physiological analyses of left-ventricular function. We found that the transplantation of contracting hESC-CM aggregates improved their survival and function in infarcted rat hearts in comparison to the transplantation of dissociated cells. Our method using hESC-CMs can be considered an effective strategy for clinical applications without critical barriers.</P>
Moon, Ji-Deok,Seon, Eun-Mi,Son, Sung-Ae,Jung, Kyoung-Hwa,Kwon, Yong-Hoon,Park, Jeong-Kil The Korean Academy of Conservative Dentistry 2015 Restorative Dentistry & Endodontics Vol.40 No.4
Objectives: This study examined the color changes of a resin composite with different shades upon exposure to water with different pH. Materials and Methods: Nanohybrid resin composites (Filtek Z350XT, 3M ESPE) with four different shades (A2, A3, B1, and B2) were immersed in water with three different pH (pH 3, 6, and 9) for 14 day. The CIE $L^*a^*b^*$ color coordinates of the specimens were evaluated before and after immersion in the solutions. The color difference (${\Delta}E^*$) and the translucency parameter (TP) were calculated using the color coordinates. Results: ${\Delta}E^*$ ranged from 0.33 to 1.58, and the values were affected significantly by the pH. The specimens immersed in a pH 6 solution showed the highest ${\Delta}E^*$ values (0.87 - 1.58). The specimens with a B1 shade showed the lowest ${\Delta}E^*$ change compared to the other shades. TP ranged from 7.01 to 9.46 depending on the pH and resin shade. The TP difference between before and after immersion in the pH solutions was less than 1.0. Conclusions: The resulting change of color of the tested specimens did not appear to be clinically problematic because the color difference was < 1.6 in the acidic, neutral, and alkaline solutions regardless of the resin shade, i.e., the color change was imperceptible.
Optimization of Isoflavone Extraction from Soy Germ
Sang-Moon Bae,Chan Ho Jang,Jang-Hoon Kim,Hyun Ae Lim,Joo-Ryong Kim,Jeong Hwan Kim,Jong-Sang Kim 한국식품영양과학회 2005 Preventive Nutrition and Food Science Vol.10 No.3
Soy isoflavones have drawn much attention due to their potential to prevent breast and prostate cancers, osteoporosis, heart disease, and other postmenopausal symptoms. Soy germ is one of the richest sources of isoflavones, and thus has good potential to be used as the ingredient of health foods. This study examined the extraction rate of isoflavones from soy germ at various conditions. After the effect of extraction temperature and duration on isoflavone extraction from soy germ was examined, the optimum concentration of ethanol as extraction solvent was determined. When ethanol concentration was fixed at 60% (v/v), the maximum isoflavone extraction was achieved at 2 hrs and 30℃. Among various concentrations of ethanol tested, 80% (v/v) ethanol showed the highest extraction efficiency. In conclusion, the maximum extraction of isoflavones was obtained using 80% (v/v) ethanol as a solvent, at 30℃ of temperature, and 2 hrs of extraction time.
Aberrant Promoter Hypomethylation of Sortilin 1: A Moyamoya Disease Biomarker
Sung, Hye Youn,Lee, Ji Yeoun,Park, Ae Kyung,Moon, Youn Joo,Jo, Inho,Park, Eun-Mi,Wang, Kyu-Chang,Phi, Ji Hoon,Ahn, Jung-Hyuck,Kim, Seung-Ki Korean Stroke Society 2018 Journal of stroke Vol.20 No.3
<P><B>Background and Purpose</B></P><P>The pathogenesis of moyamoya disease (MMD) remains poorly understood, and no reliable molecular biomarkers for MMD have been identified to date. The present study aimed to identify epigenetic biomarkers for use in the diagnosis of MMD. </P><P><B>Methods</B></P><P>We performed integrated analyses of gene expression profiles and DNA methylation profiles in endothelial colony forming cells (ECFCs) from three patients with MMD and two healthy individuals. Candidate gene mRNA expression and DNA methylation status were further validated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and pyrosequencing analysis of an expanded ECFC sample set from nine patients with MMD and ten controls. We evaluated the diagnostic accuracy of the potential biomarkers identified here using receiver operating characteristic curve analyses and further measured major angiogenic factor expression levels using a tube formation assay and RT-qPCR. </P><P><B>Results</B></P><P>Five candidate genes were selected via integrated analysis; all five were upregulated by hypomethylation of specific promoter CpG sites. After further validation in an expanded sample set, we identified a candidate biomarker gene, sortilin 1 (<I>SORT1</I>). DNA methylation status at a specific <I>SORT1</I> promoter CpG site in ECFCs readily distinguished patients with MMD from the normal controls with high accuracy (area under the curve 0.98, sensitivity 83.33%, specificity 100%). Furthermore, <I>SORT1</I> overexpression suppressed endothelial cell tube formation and modulated major angiogenic factor and matrix metalloproteinase-9 expression, implying <I>SORT1</I> involvement in MMD pathogenesis. </P><P><B>Conclusions</B></P><P>Our findings suggest that DNA methylation status at the <I>SORT1</I> promoter CpG site may be a potential biomarker for MMD.</P>