Accuracy of Ultrasonography in Early Pregnancy Diagnosis in Doe

Singh, N.S.,Gawande, P.G.,Mishra, O.P.,Nema, R.K.,Mishra, U.K.,Singh, Mohan Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.6

The present study was undertaken to evaluate the accuracy of ultrasonography in early pregnancy diagnosis in goats. Ultrasonographic scanning with real time B-mode ultrasound machine having 5 MHz linear array transducer was performed on gravid uterus (n=24) obtained from slaughterhouse (Group I). Crown rump length (CRL) measured by ultrasound was found significantly different (p<0.05) with actual CRL measured after dissection in early pregnancy. However, age predicted by ultrasound through the measurement of CRL was found highly correlated (r=0.92) with age measured after dissection through CRL and the weight of fetus. Ages predicted by ultrasound through the measurement of trunk diameter (TD) and uterine diameter (UD) and ages measured after dissection were found highly and equally correlated (r=0.98) and did not differ significantly. Data from six does synchronized (Group II) with PGF2$\alpha$ (Estrumate) at 11 days apart were collected through ultrasound from 17 to 42 days post breding. The correlation between CRL and gestational age was high (r=0.97) in day 30 to 42 post breeding. A high coefficient of correlation (r=0.98) was also observed between predicated age by ultrasound and actual age calculated after kidding. The correlation between CRL and gestational age by the formula Y=(a+bX) i.e. Y=24.42+0.39 X where Y=gestational age and X=CRL, was recorded very high (r=0.99). Accuracy of ultrasonography was lowest on day 17 to 19 (66%) and reached 100% on day 34. Data from 30 does (group III) randomly subjected to only one time ultrasounds scanning to assess the accuracy of pregnancy diagnosis were also obtained. Ages predicted by TD and UD measurements were observed to be non-significantly different with actual age obtained after kidding and correlation between ages predicted by TD and UD measurement with actual age after kidding was found equally and highly correlated (r=0.98). The operator's accuracy in the whole experiment including all three groups was found to be 92%. The sensitivity was 93% and specificity was 86%. From the present study, it was observed that CRL was the most reliable parameter to find out gestational age in early pregnancy and the new formula derived was found very accurate to find out gestational age. TD and UD were also found to be equally reliable parameter to find out gestational age in mid and late stage of pregnancy through ultrasonography. It was concluded that ultrasonography by real time B mode with 5 MHz transrectal transducer was found to be reliable, safe and accurate and practicable means in diagnosing early pregnancy diagnosis as early as 25 days post breeding.

Phenotypic and genotypic correlates of daptomycin-resistant methicillin-susceptible Staphylococcus aureus clinical isolates

강경미,Nagendra N. Mishra,박건택,이기용,박용호,Arnold S. Bayer,양수진 한국미생물학회 2017 The journal of microbiology Vol.55 No.2

Daptomycin (DAP) has potent activity in vitro and in vivo against both methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) strains. DAP-resistance (DAP-R) in S. aureus has been mainly observed in MRSA strains, and has been linked to single nucleotide polymorphisms (SNPs) within the mprF gene leading to altered cell membrane (CM) phospholipid (PL) profiles, enhanced positive surface charge, and changes in CM fluidity. The current study was designed to delineate whether these same genotypic and phenotypic perturbations are demonstrated in clinically-derived DAP-R MSSA strains. We used three isogenic DAP-susceptible (DAP-S)/DAP-R strainpairs and compared: (i) presence of mprF SNPs, (ii) temporal expression profiles of the two key determinants (mprF and dltABCD) of net positive surface charge, (iii) increased production of mprF-dependent lysinylated-phosphatidylglycerol (L-PG), (iv) positive surface charge assays, and (v) susceptibility to cationic host defense peptides (HDPs) of neutrophil and platelet origins. Similar to prior data in MRSA, DAP-R (vs DAP-S) MSSA strains exhibited hallmark hot-spot SNPs in mprF, enhanced and dysregulated expression of both mprF and dltA, L-PG overproduction, HDP resistance and enhanced positive surface charge profiles. However, in contrast to most DAP-R MRSA strains, there were no changes in CM fluidity seen. Thus, charge repulsion via mprF- and dlt-mediated enhancement of positive surface charge may be the main mechanism to explain DAP-R in MSSA strains.

ON GENERALIZED HARMONIC VECTOR VARIATIONAL INEQUALITIES USING $HC_*$-CONDITION

Mishra S. N.,Das P. K.,Mishra S. K. 경남대학교 기초과학연구소 2019 Nonlinear Functional Analysis and Applications Vol.24 No.3

In this paper some results on $HC_*$-condition are established in the harmonic invex set and are used to establish the existence theorem of the solution of the generalized harmonic variational inequalities and its dual problem using generalized harmonically monotone property of the operator.

A Dielectric-Modulated Normally-Off AlGaN/GaN MOSHEMT for Bio-Sensing Application: Analytical Modeling Study and Sensitivity Analysis

S. N. Mishra,K. Jena 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.74 No.4

This paper presents an analytical model of a bio-molecule-induced threshold voltage shift (Vth) in a normally-o AlGaN/GaN metal-oxide-semiconductor high-electron-mobility transistors (MOSHEMTs) used as bio-particle sensors. In the analytical model, the presence of biomolecules is represented by using the dielectric modulation (DM) technique for label-free electrical detection. The dielectric/semiconductor interface density-of-State (DOS)-dependent model for the density of two dimensional electron gas (2DEG) is obtained by solving a 2-D Poisson equation demonstrating required energy band diagrams. The eective capacitance in the cavity region and the threshold voltage are obtained by using dielectric modulation and the Poisson equation. Subsequently, the changes in the threshold voltage and the drain current of the device are used as the sensing metric for the detection of bio-molecules in the cavity region. Interestingly, the charge of the biomolecules can also used as a sensing parameter. The predicted sensing metric characteristics of the devel- oped analytical model are in good agreement with the result of technology computer-aided design (TCAD) simulations, thus conrming the validity of the proposed model.

HARMONIC INVEX SETS AND GENERALIZED HARMONIC VARIATIONAL INEQUALITY PROBLEMS

S. N. Mishra,P. K. Das,G. C. Nayak 경남대학교 기초과학연구소 2018 Nonlinear Functional Analysis and Applications Vol.23 No.1

In this paper, we have defined various type of generalized harmonic variationalinequality problems in harmonic invex set and studied their existence theorems under certainconditions.

Microstructure, dielectric relaxation, optical, and ferroelectric studies of a lead-free double perovskite: BaLiFeMoO6

Mishra S.,Choudhary R. N. P.,Parida S. K. 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.2

In this article, the influence of co-substitution of alkali and transition metals at the Ba/Mo site of host BaMoO4 (inorganic Scheelite) is reported. A new lead-free double perovskite BaLiFeMoO6 (BLFMO) is synthesized using a conventional solid-state reaction route, having tetragonal crystal symmetry with an average crystallite size of 93.2 nm and strain of 0.115%. Grains are grown by sintering and distributed uniformly through well-defined grain boundaries as obtained from scanning electron microscopy (SEM). The purity and compositional analysis through energy-dispersive analysis X-ray spectroscopy (EDX) technique confirms the presence of constituent elements Ba, Li, Fe, Mo, and O. Raman study supports the substitution of Li/Fe atoms at Ba and Mo sites in the host matrix. Direct bandgap energy of 2.99 eV is calibrated using the ultraviolet–visible (UV–Vis) spectrum; may be suitable for photovoltaic applications. Maxwell–Wagner dielectric response, non-Debye relaxation, activation energy, and negative temperature coefficient resistance (NTCR) behavior are the outcomes of the synthesized sample characterized using dielectric, impedance, modulus, and conductivity plots in a wider spectrum of frequency (1 kHz–1 MHz) and temperatures (25–500 °C). The contribution of both grains and grain boundaries in the conduction mechanism has been investigated using Nyquist's fitting data calibrated from ZSIMPWIN software. A decrease in the magnitude of grain boundary resistance with a rise in temperature in fitted Nyquist's plot suggests the presence of semiconducting nature in the material. Analysis of leakage current density suggests that the nature of the conduction mechanism follows to the space charge limited current process. The study of temperature-dependent resistance supports BLFMO as a good candidate for NTC thermistor-related device applications. The study of polarization–electric field hysteresis loop suggests the possibility of the ferroelectric nature in the studied sample.

Ratcheting Fatigue Behavior of Modified 9Cr–1Mo Steel at Room Temperature

Prerna Mishra,R. S. Rajpurohit,N. C. Santhi Srinivas,G. V. S. Sastry,Vakil Singh 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

Fusion reactor components undergo asymmetric cyclic loading,that leads to a progressive increase in plastic strain andcauses deterioration in life of engineering components. This investigation deals with fatigue behavior of modified 9Cr–1Mosteel at room temperature and presents the effect of mean stress (σm), stress amplitude (σa) and stress rate ( ̇ ) on fatigue life,deformation and fracture behavior under asymmetric cyclic loading. A series of fatigue tests were conducted under asymmetricstress-controlled loading with different combinations of σm (190–210 MPa), σa (400–420 MPa) and ̇ (50–450 MPa/s). The plastic strain increased with increase in σm and σa and the fatigue life was reduced, whereas increase in ̇ reduced theaccumulated plastic strain and the cyclic life was increased. The deformation behavior and microstructural changes under theinfluence of the three parameters (σm, σa and ̇ ) were examined by tranmission electron microscope (TEM). With increasein the three parameters (σm, σa, ̇ ), the lath martensitic structure changed to subgrain structure along with dislocation cellsand forest dislocations. Scanning electron microscopy revealed unique features of fractured specimens, with progressivereduction in diameter towards the fracture-end, fatigue striations on the tapered circumferential surface and dimples on thefracture surface.

Robust Features for Connected Hindi Digits Recognition

A. N. Mishra,Mahesh Chandra,Astik Biswas,S. N. Sharan 보안공학연구지원센터(IJSIP) 2011 International Journal of Signal Processing, Image Vol.4 No.2

Ratcheting Fatigue Behaviour of Zircaloy‑2 at 300 °C

R. S. Rajpurohit,Prerna Mishra,N. C. Santhi Srinivas,S. R. Singh,V. Singh 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.9

In laboratory, materials designed for engineering applications, specifically for fatigue, are generally tested under symmetricalcyclic loading (stress ratio, R = −1), but many structural components exhibit less fatigue life than predicted from symmetricloading due to asymmetric cyclic loading during service. This study deals with fatigue behaviour of Zircaloy-2 and presentsthe effect of mean stress (σm), stress amplitude (σa), stress rate ( ̇ ) on fatigue life, deformation and fracture behaviour at300 °C under asymmetric cyclic loading. A series of fatigue tests are performed at 300 °C under asymmetric stress-controlledloading with different combinations of σm (60–80 MPa), σa (115–135 MPa) and ̇ (30–750 MPa/s). Deformation behaviourand microstructural changes under the influence of above parameters (σm, σa and ̇ ) are examined by transmission electronmicroscope. It is observed that plastic strain increases with rise in σm as well as σa and cyclic life is reduced; on the otherhand, with increase in ̇ accumulation of plastic strain decreases and fatigue life is enhanced. The results are correlated withmicrostructural changes and fracture behaviour of the material under different test conditions.

Cp*Rh(III)-catalyzed C(sp3)-H alkylation of 8-methylquinolines in aqueous media

Kim, S.,Han, S.,Park, J.,Sharma, S.,Mishra, N.,Oh, H.,Kwak, J.,Kim, I. Royal Society of Chemistry 2017 Chemical communications Vol.53 No.21

<P>The rhodium(III)-catalyzed cross-coupling reaction of 8-methylquinolines with a range of allylic alcohols in water is described. This approach leads to the synthesis of various c-quinolinyl carbonyl compounds, which are synthetically useful precursors for the construction of bioactive tetrahydroquinoline and azasteroid derivatives.</P>