다양한 전처리 방법에 따른 양파의 이화학 및 영양학적 특성 분석

김광일 ( Kwang Il Kim ),황인국 ( In Guk Hwang ),유선미 ( Seon Mi Yu ),민상기 ( Sang Gi Min ),이상윤 ( Sang Yoon Lee ),최미정 ( Mi Jung Cho ) 한국산업식품공학회 2014 산업 식품공학 Vol.18 No.4

본 연구는 양파를 열처리 법 중에서 열수침지, 증기 및 볶음 처리에 따른 이화학적 및 영양학적 특성을 분석한 논문으로 열처리가 양파에 미치는 영향을 비교하고자 하였다. 상품을 구매할 때 1차적인 구매요소인 색도 및 경도측정과 추가적으로 pH와 조직관찰 결과로 최적조건 선정 후 영양학적 및 미생물 검사를 실시하였다. 열처리를 하지 않은 대조구에 비해 짧은 시간의 열처리에서는 더 진하고 선명한 색을 띠었으나, 장시간동안 처리한 열처리 시료는 색의 침착을 보였다. 원물과 처리구의 색은 열처리 시간에 따라 뚜렷한 차이를 나타냈고, 경도는 열처리 시간에 따라 짧은 시간에서는 분해효소의 불활성화로 유지 및 증가했지만, 3분 이상 처리하였을 때는 감소 경향을 나타냈고, 볶음처리는 짧은 시간으로도 물성이 연화됐다. 비타민은 열수 및 증기처리 1~2분 처리는 14% 정도 감소했지만 그 이상 처리 시에는 23~28% 범위로 큰 감소율을 보였으며, 유리당 및 유기산의 경우 열수침지 및 증기처리에서 손실을 보였지만, 볶음처리에서는 유지하거나 증가하는 경향을 나타냈다. 특히 유리당에서 열수침지 처리 시 높은 감소율을 나타냈다. Peroxidase activity 변화는 모든 처리구에서 75% 이상의 불활성화를 나타냈고, 특히 열수침지 및 증기 처리한 모든 시료에서 90% 이상의 불활성화를 나타내며 볶음처리보다 높은 불활성화율을 보였다. 미생물 측정한 결과 열수 침지 3분 이상 및 볶음처리 210초 이상 처리시 미생물 검출이 안됐으며, 증기처리 시료는 2분 처리만으로 사멸된 것을 관찰했다. 양파의 열처리 조건은 증기처리법으로 2분 동안 처리하는 것이 다른 처리법에서 처리한 시간들에 비해 최적으로 나타났다. 열처리 방법 별 각각의 최적 처리 시간을 가지고 있어, 높은 효율성이나 산업적으로 이용하려면 최적의 전처리 방법과 처리 시간을 산정하는 것이 중요하다. The pre-thermal treatment, or blanching, is an important process to minimize quality changes during main cooking or storage. In this study, three types of pre-thermal treatments were applied to onions to observe quality changes in their physicochemical, nutritional, and microbial properties. The washed and sliced onions were treated by hot-water immersion (100oC, 1~10 min), steaming (100oC, 1~10 min) and stir-frying with oil (180oC, 10~80 sec). The onions were cooled and wiped with gauze. The results showed that the total color difference of the treated sample increased to 18-23 units compared to that of the fresh control. The pH value of the onion was not significantly different with the exception of the stir-fried samples. The hardness of the onion tended to decrease depending on the treatment time. The micro-structures of onions treated by hot-water immersion and steaming were damaged above 2 min whereas stir-fried onions showed structural damage above 210 sec. In the analysis of the nutritional changes in the onion, the ascorbic acid content of the blanched treatments were lower than that of fresh onion and the free sugar contests showed a similar pattern excluding the stir-fried onions. In the organic acid content assay, the succinic acid content of the treated onion sample had significantly decreased compared with other organic acid contents (p<0.05). The peroxidase activities of the blanched treatments by hot-water immersion and steaming were inactivated by more than 92%, whereas only 76% inactivity was achieved by the stir-frying treatment. The mesophilic and psychrotrophic bacteria counts of the treated onion were not detected or decreased to near a 3-log scale, and yeast was detected only the sample treated by the stir-frying for 1 min. In particular, neither bacteria nor yeast counts in all onion samples by steam treatment were detected. Consequently, the results demonstrated that steam treatment for 2 min could be the optimal pre-thermal condition for onions to minimize the quality changes and increase safety.

殺蟲性 結晶蛋白質 特異的인 抗體의 生産

김기윤,이정민,유형진,백길현,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.1

Bacillus thuringiensis is a gram-positive soil bacterium characterized by its ability to produce crystalline inclusions during sporulation. These parasporal bodies consist of protoxins known as crystal proteins exhibiting highly specific insecticidal activities. The Lepidoptera-specific protoxin, Cry IAc, was purified from E.coli JM103 harboring the cry IAc gene. The gene was isolated from Bacillus thuringiensis kurstaki HD-73 and subcloned into an expression vector, pKK223-3. The recombinant DNA(pOS4201) was transformed into E.coli JM103. The Cry I Ac protoxin overexpressed in the E.coli was isolated from the total proteins by differential solubility. The protoxin were trypsinized to obtain activated toxin. The proteins was resolved on SDS gel. The molecular weights of the pro-and toxin were resistered about 130,000 and 65,000 Daltons respectively. The Cry IAc toxin was used as immunogen to produce anti-Cry IAc antiserum in a rabbit. The immunogen (200㎍/ 200㎕) was mixed with an equal volume of Freund's complete adjuvant, and the mixture was hypodermically injected to the hide back (30 spots) and paws (2 spots) of the rabbit. The immunization was performed four times every two weeks. The dosage of the immunogen was decreased by half, and incomplete adjuvant was substituted for the complete one from the second immunization. The serum was prepared from the blood as described elsewhere. Immunoreactivity of the serum was examined by dot-blotting with the aid of GAR-HRP indirect immunoassay kit. It has been found that the anti-Cry I Ac antiserum recognized nano-gram quantity of antigen under these experimental conditions. The antiserum will he used for the development of immunochemical mean(s) to screen transgenic plants transformed by the insecticidal characteristics of Cry I Ac.

새로운 Acetylcholinesterase inhibitors의 합성

최순규,김형민,조승환,최학기,박유미,이용균,정대일,김인식,한정태 동아대학교 부설 기초과학연구소 2004 基礎科學硏究論文集 Vol.21 No.1

노인성 치매의 일종인 alzheimer's disease의 효과적인 억제제를 합성하기 위해 우리는 분자 모델링에 의한 가장 적합한 물질인 m-[(N,N,N-trimethylammonio)phenyl]boronic acid를 합성하였다. 출발물질인 3-aminophenylboronic acid monohydrate의 경우 boronic acid의 작용기인 hydroxyl group을 protecting시킴으로써 반응의 안정성을 기하였다. Quarternary ammonium salt는 과량의 methyliodide와 염기 촉매인 potassium hydrogen carbonate를 사용하여 용매인 methanol에서 반응시켜 상당히 높은 수율을 얻을 수 있었다. 또한 과량의 methyliodide와 염기촉매인 potassium hydrogen carbonate를 사용하여 용매인 methanol에서 반응시킨 결과 boronic acid의 protection없이도 안정하게 반응이 진행되어짐을 확인할 수 있었다. In order to syntheisize a effective inhibotor for alzheimer's disease, we synthesized m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4 which is designed by molecular modeling form. We protected the hydroxyl group of 3-aminophenylboronic acid monohydrate 1 with ethlyne glycol to remove the reactivity if hydroxyl group. To synthesize m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4, we reacted 3-aminophenylboronic acid monohydrate 1 with ezcess methyl iodide and potassium hydrogen carbonate as a base-catalyst in methanol. but On executed reaction without protection on hydroxyl group, we found out the result that the hydroxyl group of boronic acid group at 3-aminophenylboronic acid monohydrate 1 didn't react with excess methyliodide. Synthesized m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4 is in progress about biological tests as a plausible acetylcholinesterase inhibitor.

Ethanosolv 전처리에 의한 보릿짚의 리그닌 제거

김영란(Youngran Kim),유안나(Anna Yu),정봉우(Bongwoo Chung),한민희(Min-hee Han),최기욱(Gi-wook Choi) 한국생물공학회 2009 KSBB Journal Vol.24 No.6

본 연구에서는 바이오 에탄올 생산을 위한 보릿짚의 전처리에 ethanosolv 방법을 적용하여 그 타당성을 조사 하였다. 리그닌제거율은 처리 온도와 시간에 따라 증가 하였으며 180℃, 200℃에서 거의 38%정도의 비슷한 제거율을 보였는데 비용절감 등의 부분을 감안할 때 80℃, 120 min가 적정조건이라는 결론을 얻었다. ethanosolv 전처리 효과를 증대시키기 위하여 2단계의 전처리 방법을 적용하였다. 볶은 후 ethanosolv 한 보릿짚의 경우 리그닌 제거율은 35%정도로 그렇지 않은 경우와 거의 유사하여 볶음이 리그닌제거율에 큰 영향을 미치지 않음을 확인 할 수 있었다. XRD분석을 통하여 전처리 시간과 온도가 증가할수록 결정성은 감소하였다. 볶은 후 ethanosolv 한 것과 ethanosolv 단독 처리한 보릿짚 사이의 결정성은 미소 하지만 물리적 변형을 한 단계 더 겪은 볶은 보릿짚이 전체적으로 낮게 나타났다. Lignocellulose represents a key sustainable source of biomass for transformation into biofuels and bio-based products. Unfortunately, lignocellulosic biomass is highly recalcitrant to biotransformation, both microbial and enzymatic, which limits its use and prevents. As a result, effective pretreatment strategies are necessary. The vast majority of pretreatment strategies have focused on achieving a reduction of lignin content. In this work, an ethanosolv pretreatment has been evaluated for extracting lignin from barley straw. 75% ethanol was used as a pretreatment solvent to extract lignin from barley straw. The influence on delignification of three independent variables are temperature, time, catalyst (1 M H₂SO₄) dose. The best pretreatment condition observed was 180℃, 120 min, 0.2% H₂SO₄ and delignification was 38%. A combined roasting and ethanosolv, 2-step pretreatment, was developed in order to improve the delignification. Roasting didn"t increase the delignification but reduced the pretreatment time. X-ray diffraction results indicated that these physical changes enhance the enzymatic digestibility in the ethanosolv treated barley straw. The cellulose in the pretreated barley straw becomes more crystalline without undergoing ethanosolv.

Research Articles : Quality Characteristics of Frozen Beef Developed into Home Meal Replacement Products Under Various Storage Conditions

( Sangyoon Lee ),( Kwang Il Kim ),( In Guk Hwang ),( Seon Mi Yu ),( Sang Gi Min ),( Mi Jung Choi ) 한국산업식품공학회 2015 산업 식품공학 Vol.19 No.3

Blanching and freezing of beef are important techniques used in developing frozen home meal replacement (HMR) products. These pre-thermal treatments minimize the deterioration of beef during storage. Beefs are washed, defatted, sliced (5.0×0.5×0.5 cm, fiber direction), and blanched by immersion in hot water for 2 min. Thereafter, the blanched beefs are spin-dried and frozen by individual quick freezing (IQF) method. Finally, the frozen beefs are packed using two methods: vacuum packages and air-containing packages. Packed beefs are stored at -12℃, -18℃, and -24℃ for nine months. One in every three months, they are thawed in a microwave (400W). While thawing, vacuum- packed samples possess lower thawing loss than air-containing-packed ones. Compared to raw beef, blanched beef have a higher L* and lower a* value. However, less changes are observed after blanching the beefs as the enzymes get inactivated in the blanching process. Shear force of beef also increase through blanching. Moreover, vacuum-packed samples have maintained their hardness to a greater extent than the air-containing-packed samples. All the samples have met microbial safety standards. Thus, it is inferred that vacuum-packaging and -18℃ storage temperatures are the best condition for maintaining beef that is later developed into HMR product.

IVIG Treatment Response and Age are Important for the Prognosis of Pediatric Immune Thrombocytopenia

Min Gi Sakong,Ji Hoon Park,Sang Beom Son,Yu Kyung Kim,Jae Min Lee 대한소아혈액종양학회 2022 Clinical Pediatric Hematology-Oncology Vol.29 No.2

Background: This study aimed to identify chronicity predictors of pediatric primary immune thrombocytopenia (ITP).Methods: This study retrospectively reviewed the medical records of patients with pri-mary pediatric ITP admitted to a tertiary medical center between 2010 and 2021. Forty-five patients with a platelet count <20,000/μL at the time of diagnosis who were treated with intravenous immunoglobulin (IVIG) were enrolled in this study.Results: According to the disease phase, 28, 6, and 11 patients were classified into the newly diagnosed (ND), persistent, and chronic groups, respectively. The number of patients over 6 years of age was significantly higher in the chronic group than in the ND and persistent groups. After 14 days of IVIG treatment, more patients had a complete response in the non-chronic groups than in the chronic group. In uni-variate analysis of risk factor for chronic ITP, age 6 years or older and IVIG dose <2 g/kg were found to be risk factors for chronic ITP, and these two factors were also confirmed as significant risk factors in multivariate analysis.Conclusion: In conclusion, in our study, patients over 6 years of age, and those who received IVIG doses less than 2 g/kg were more likely to progress to chronic ITP.

결정핵 물질 투입을 따른 축산폐수로부터의 Struvite 결정화 영향

유민성 ( Min Sung Yu ),김대기 ( Dae Gi Kim ),민경진 ( Kyung Jin Min ),박기영 ( Ki Young Park ) 한국물환경학회(구 한국수질보전학회) 2015 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2015 No.-

스마트폰과 고니오메타를 이용한 어깨관절의 안쪽돌림과 바깥돌림의 신뢰도와 타당도

정민기 ( Min-gi Jung ),임나영 ( Na-young Im ),최유리 ( Yu-ri Choi ),황현지 ( Hyun-ji Hwang ),조아영 ( A-young Cho ),추연기 ( Yeon-gi Choo ),오태영 ( Tae-young Oh ) 대한신경치료학회 2016 신경치료 Vol.20 No.2

Purpose This research was performed to inquire the reliability and validity of internal rotation and external rotation of shoulder joint using smart phone and goniometer to healthy subjects. Methods Universal goniometer and two android application, hysical tools YMED ROM and goniometer developed by Nagomi.aqu was used to collect the data from 30 healthy subjects in age of 20s, 15 male and 15 female, from S University in Busan, Republic of Korea. As a procedure, all subjects were properly stretched before the experiment. The active internal and external rotation of right arm of each subject were measured and 3 raters measured each subject 5 times with 3 different tools, overall 15 times for each subject. And each rater were blindfolded to conceal the results from other raters. SPSS 20.0 software were used to process all statistics, and intraclass correlation coeffienct (2.k) model were used to calculate the reliability and pearson correlation coefficient validity were used to calculate the validity. Results As the result, intrarater reliability of 3 raters showed high reliability (ICC>0.9). Interrater reliability showed high reliability in all measurement (ICC>0.9), validity of measuring tools showed very high correlation in both active internal and external rotation of shoulder joint. Conclusion This research showed the value measured with goniometer application using gyroscope of smartphone is reliable. Peculiarly, the value of both reliability and validity of external rotation was higher than that of internal rotation

구강암 환자에서 18F FDG-PET/CT의 경부 림프절 전이 평가 유용성

유민기(Min-gi Yu),유선열(Sun-Youl Ryu) 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.4

Purpose: The present study was aimed to examine the usefulness of 18F-FDG PET/CT in the evaluation of cervical lymph node metastasis in patients with oral cancer. Materials and methods: Twenty-two patients who underwent neck dissection to treat oral cancer were subjected for examination. The cervical node metastasis was evaluated by means of clinical examination, CT scan, PET, and histologic examination. By comparing the results of each examination modality with those of histologic examination, it s sensitivity, specificity, positive predictive value, and negative predictive value were determined. Results: The oral cancer was more frequent in males with a ratio of 2.14:1. The sixth decade showed the highest incidence in age distribution with mean of 56±16. Histologic findings showed that squamous cell carcinoma was the most common (15 patients), and mucoepidermoid carcinoma (3), malignant melanoma (2), and adenoid cystic carcinoma and ghost cell odontogenic carcinoma (1 each), in order. In most cases, wide surgical excision of the primary cancer and neck dissection was performed, followed by reconstruction with free flaps when necessary. When comparing the results of each examination modality with those of the histologic examination, clinical examination showed sensitivity, specificity, positive predictive value, and negative predictive value at 11%, 85%, 33%, and 58%, respectively. CT scans showed at 67%, 77%, 67%, and 77%, while 18F-FDG PET/CT at 78%, 77%, 70%, and 83%, respectively. Conclusions: These results suggest that PET is more useful, compared with clinical examination and CT scans, in the evaluation of cervical lymph node metastasis in patients with oral cancer.

Magnetic Properties and Giant Magnetocaloric Effects on Gd-Ge-Si Alloy by Substitution of Sn

Seong-Gi Min,Ying De Zhang,Chul-Min Heo,Xueling Hou,Ying Li,Seong-Cho Yu,Kyu- Won Lee 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-