Enhanced Catalytic Ability of Candida rugosa Lipase Immobilized on Pore-enlarged Hollow Silica Microspheres and Cross-linked by Modified Dextran in Both Aqueous and Non-aqueous Phases

Liwei Ren,Honghua Jia,Min Yu,Wenzhong Shen,Hua Zhou,Ping Wei 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.5

Candida rugosa lipase (CRL) is one of the most widely used lipases. To enhance the catalytic abilities of CRL in both aqueous and non-aqueous phases, hollow silica microspheres (HSMSs) with a pore size of 18.07 nm were used as an immobilization support, and aldehydecontaining dextrans were employed to further cross-link the adsorbed CRL. In the experimental ranges examined,the loading amount of lipase linearly increased to 171 ±3.4 mgprotein/gsupport with the CRL concentration and all the adsorption equilibriums were reached within 30 min. After simple cross-linking, the tolerance to pH 4.0 ~ 8.0 as well as the thermal stability of immobilized CRL at 40 ~ 80oC were both substantially increased, and 82 ± 2.1% activity remaining after the sixth reuse. The immobilized CRL was successfully applied to the resolution of racemic ibuprofen in non-aqueous phase. The initial reaction rate increased by 1.4- and 3.6-fold compared with the rates of adsorbed and native lipases, respectively. Furthermore, the R-ibuprofen was obtained at ee > 93%, and the enantiomeric ratio reached E > 140 at the conversion of 50 ± 1.5% within 48 h.

Effects of zearalenone on the localization and expression of the growth hormone receptor gene in the uteri of post-weaning piglets

Min Zhou,Li Jie Yang,Wei Ren Yang,Li Bo Huang,Xue Mei Zhou,Shu Zhen Jiang,Zai Bin Yang 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.1

Objective: In this study, we investigated the adverse effects of dietary zearalenone (ZEA) (0.5 to 1.5 mg/kg diet) on the localization and expression of the growth hormone receptor (GHR) in the uteri of post-weaning gilts and explored alternative mechanism of the reproductive toxicity of ZEA on piglets. Methods: A total of forty healthy piglets (Duroc×Landrace×Large White) aged 28 d were selected for study. Piglets were transferred to single cages after 10 days’ adaptation on an obstetric table. The animals were allocated to one of four treatments: a normal basal diet supplemented with 0 (Control), 0.5 (ZEA0.5), 1.0 (ZEA1.0), or 1.5 (ZEA1.5) mg/kg purified ZEA, and fed for 35 d after the 10-d adaptation. Analyzed ZEA concentrations in the diets were 0, 0.52±0.07, 1.04±0.03, and 1.51±0.13 mg/kg, respectively. At the end of the feeding trial, piglets were euthanized after being fasted for 12 h. Two samples of uterine tissue from each pig were rapidly collected, one of which was stored at –80°C for analysis of the relative mRNA and protein expression of GHR, and the second was promptly fixed in Bouin’s solution for immunohistochemical analysis. Results: The relative weight of the uteri and thickness of the myometrium and endometrium increased linearly (p<0.001) and quadratically (p<0.001) with an increasing level of ZEA. The results of immunohistochemical analysis indicated that GHR immunoreactive substance was mainly localizated in the cytoplasm of uterine smooth muscle, glandular epithelial, luminal epithelial, stromal, and vascular endothelial cells. In contrast, nuclear staining was rarely observed. The immunoreactive integrated optic density of GHR in the myometrium, luminal epithelium, glandular epithelium, and whole uteri of weaning gilts increased linearly (p<0.001) and quadratically (p<0.05) with an increasing level of ZEA. The mRNA and protein expression of GHR in the uteri of weaning gilts increased linearly (p<0.001) and quadratically (p<0.05) with an increasing level of ZEA. Conclusion: In conclusion, ZEA at a concentration of 0.5 mg/kg was sufficient to significantly thicken the myometrium and endometrium, and at a concentration of 1.0 mg/kg induced a high level of GHR expression to promote growth and development of the uteri. This revealed an alternative molecular mechanism whereby ZEA induces growth and development of the uteri and provides a theoretical basis for the revision of Chinese feed hygiene standards.

진로장벽과 진로결정 자기효능감 및 진로준비행동 간의 관계- 중국 랴오닝 지역 대학생을 중심으로 -

Zhou Min,송태임 한국의류산업학회 2023 한국의류산업학회지 Vol.25 No.2

This study attempts to reveal the causal relationship between certain variables, with the objective being toinvestigate the influence of career disability on self-efficacy and career preparation behavior of Chinese college students. In terms of data collection, online surveys were conducted during the period spanning September 1 to September 20,2022 targeting fourth-year university students living in Liaoning, China. Atotalof536completedsurveyswere used for thefinal analysis, and the SPSS 25.0 program was employed in this regard. The results of the study confirmed that the careerbarriers of Chinese college students had both positive and negative effects on career decision-making self-efficacy andcareer preparation behavior, signaling a partial causal relationship. This indicates that career barriers are not only negativefactors in career decision-making or career preparation. The current study is meaningful in that the results can serve asthe basis for future studies, as the work itself investigated the influence of career barriers on career decision self-efficacyand career preparation behavior; additionally, the results were quantified. It is anticipated that there will be further studieson such influences considering the diverse variables of career barriers, career decision self-efficacy, and career preparationbehavior. The results of this study are expected to provide useful information on career preparation to Chinese collegestudents – information which can be used as empirical data.

Molecular design and synthesis of D-π-A structured porphyrin dyes with various acceptor units for dye-sensitized solar cells

Zhou, Haoran,Ji, Jung-Min,Kang, Sung Ho,Kim, Min Su,Lee, Hyun Seok,Kim, Chul Hoon,Kim, Hwan Kyu The Royal Society of Chemistry 2019 Journal of Materials Chemistry C Vol.7 No.10

<P>Two porphyrin sensitizers, SGT-023 and SGT-025, were synthesized through acceptor structural engineering. SGT-023 was designed by replacing the commonly used benzothiadiazole (BTD) unit in the well-known platform of D-porphyrin-triple bond-BTD-acceptor sensitizers (<I>e.g.</I>, SM315 and SGT-021) with a pyridothiadiazole unit as a stronger electron-withdrawing group. As for SGT-025, an additional ethynylene bridge was inserted into the acceptor part of the well-known skeleton of D-porphyrin-triple bond-BTD-acceptor sensitizers. The impact of the auxiliary acceptors on the optical, electrochemical, and photovoltaic properties was investigated and compared with a benchmark dye, SGT-021, developed by our group. Consequently, both porphyrin sensitizers could red-shift the absorption range and downshift the lowest unoccupied molecular orbital (LUMO) energy level, which was supposed to achieve higher light harvest efficiency (LHE). Under standard global AM 1.5G solar light conditions, SGT-025 attained a relatively high maximum power conversion efficiency (PCE) of 11.0%, which was slightly lower than that of the reference dye SGT-021 (12.6%), and a moderate PCE of 5.6% was obtained by SGT-023. The main reason is the lower charge collection efficiency, which can be attributed to the tilted dye adsorption mode on TiO2. This may allow for faster charge recombination which eventually leads to lower <I>J</I>sc, <I>V</I>oc and PCE.</P>

Antitumor Effects of Hyperthermic CO₂ Pneumoperitoneum on Human Gastric Cancer Cells

Zhou, Hou-Min,Feng, Bo,Zhao, Hong-Chao,Zheng, Min-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1

Aim: To elucidate the effects of hyperthermic $CO_2$ pneumoperitoneum on human gastric AGS cells. Methods: Based on a newly devised in vitro study model, we evaluated the anti-cancer effects of HT-$CO_2$ ($42-44^{\circ}C$ for 2-4h) on human gastric cancer cells, and also the corresponding mechanisms. Results: HT-$CO_2$ ($42-44^{\circ}C$ for 2-4h) severely inhibited cell proliferation as assessed by Cell Counting Kit-8 assay, while inducing apoptosis in a temperature- and time-dependent manner demonstrated by annexin-V/PI flow cytometry and morphological analysis (Hoechst/PI fluorescence). In addition, it was found that HT-$CO_2$ ($42-44^{\circ}C$ for 2-4h) promoted the up-regulation of Bax by western blotting. Significantly, it could also suppress gastric cancer cell invasion and metastasis by in vitro invasion and motility assay. Conclusion: In conclusion, HT-$CO_2$ had an efficacious cytotoxic effect on gastric cancer cells through Bax-induced mitochondrial apoptotic signaling. Our studies indicate that it may serve as a potential therapy for peritoneal carcinomatosis of gastric cancer. Further investigations in vivo using animal models are now urgently needed.

A Survey of Motion Capture Data Earning as High Dimensional Time Series

Zhou Zhi-Min,Chen Zhong-Wen 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.9

Constructing effective and generalizable synthesized motions is crucial for creating naturalistic, versatile, and effective virtual characters and robots. High dimensional time series are endemic in applications of machine learning such as robotics (sensor data), computational biology (gene expression data), vision (video sequences) and graphics (motion capture data). Practical nonlinear probabilistic approaches to this data are required. I would like to go through several existing models such as Gaussian Process Dynamic Systems and Deep Belief Networks. I would analyze their strengths and limitations. I would also try to incorporate physical constraints to improve the motion quality. And on the other hand, try to improve the structure of the models or the learning algorithms.

Hemalin, a thrombin inhibitor isolated from the midgut EST database of hard tick Haemaphysalis longicornis

Min Liao,Jinlin Zhou,Haiyan Gong,Damdinsuren Boldbaatar,Rika Umemiya-Shirafuji,Banzragch Battur,Jianbao Dong,Yoshifumi Nishikawa,Myungjo You,Kozo Fujisaki 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

A full-length sequence of a thrombin inhibitor (designated as hemalin) from the midgut of pathenogenetic Haemaphysalis longicornis has been identified. Sequence analysis shows that this gene belongs to a Kunitz-type family, containing two Kunitz domains with high homology to boophilin, the thrombin inhibitor from Rhipicephalus (Boophilus) microplus. The recombinant protein expressed in insect cells delays bovine plasma clotting time and inhibits both thrombin-induced fibrinogen clotting and platelet aggregation. A 20-kDa protein was detected from the midgut lysate with antiserum against recombinant hemalin. The gene expresses at all stages of the tick except for the egg stage and mainly in the midgut of the female adult tick. Real-time PCR analysis shows that this gene has a distinctly high expression level in the rapid bloodsucking period of the larvae, nymphs, and adults. Disruption of the hemalin gene led to a 2-day extension of the tick blood feeding period, and 27.7% of the ticks did not successfully complete the blood feeding. These findings indicate that the newly indentified thrombin inhibitor from the midgut of H. longicornis might play an important role in tick blood feeding.

PARTICIPATION IN ANTI-JAPANESE DEMONSTRATIONS IN CHINA: EVIDENCE FROM A SURVEY ON THREE ELITE UNIVERSITIES IN BEIJING

Min Zhou,Hanning Wang 동아시아연구원 2016 Journal of East Asian Studies Vol.16 No.3

Nationwide anti-Japanese demonstrations have erupted in China periodically in recent years. This study investigates what factors make university students more motivated to participate in anti-Japanese demonstrations. We collected original data on 1,458 university students in Beijing in June 2014, inquiring about both actual and possible future participation. We find that students are more willing to participate in future demonstrations (1) when they believe that anti-Japanese demonstrations benefit China’s diplomacy (instrumentality), and (2) when they have prior demonstrators in their social networks (diffusion). However, when it comes to actual participation, only diffusion plays a significant role while instrumentality does not. While students claim that they are motivated by beliefs that demonstrations will matter for China’s diplomacy, they actually turn out only when networks operate. In addition, membership in the Chinese Communist Party (CCP) does not affect prospective participation but deters actual participation. The CCP actually discourages participation in recent anti-Japanese demonstrations.

The novel gene LRP15 is regulated by DNA methylation and confers increased efficiency of DNA repair of ultraviolet-induced DNA damage

( Zhou Min Xu ),( Wei Ran Gao ),( Qi Mei Jian ),( Chen Jing Lu ) 생화학분자생물학회 2008 BMB Reports Vol.41 No.3

A Single-phase Buck-boost AC-AC Converter with Three Legs

Zhou, Min,Sun, Yao,Su, Mei,Li, Xing,Liu, Fulin,Liu, Yonglu The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.2

This paper proposes a single-phase buck-boost AC-AC converter. It consists of three legs with six switching units (each unit is composed of an active switch and a diode) and its input and output ports share a common ground. It can provide buck-boost voltage operation and immune from shoot-through problem. Since only two switching units are involved in the current paths, the conduction losses are low, which improves the system efficiency. The operation principle of the proposed circuit is firstly presented, and then, various operation conditions are introduced to achieve different output voltages with step-changed frequencies. Additionally, the parameters design and comparative analysis of the power losses are also given. Finally, experimental results verify the correctness of the proposed converter.