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Behavioral Effect of Sterculia apetala Seed Oil Consumption in Male Zucker Rats
Socorro Herrera-Meza,Juan Francisco Rodrıguez-Landa,Armando J. Martınez,Grecia Herrera-Meza,Rafael Fernandez-Demeneghi,Karla Reyes-Saldana,Rosa Marıa Oliart-Ros 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.11
Sterculia apetala (order: Malvales, family: Sterculiaceae) seed oil contains two cyclopropene fatty acids: sterculic and malvalic acid. Both positive and negative effects have been associated with the consumption of sterculic oil. In Mexico, S. apetala seeds are consumed after being boiled or roasted, used as chocolate flavoring, and utilized as animal fodder. Therefore, it is important to evaluate whether the consumption of this seed has a negative impact on the organism. The aim of this study was to evaluate the effect of administration of sterculic oil, during an 8-week period, on anxiety-like behavior and spontaneous locomotor activity in Zucker rats, analyzed through light/dark and open-field tests. The results showed that the consumption of sterculic oil decreased exploration latency in light/dark tests, which suggests an anxiolytic-like effect. Alterations in time spent on rearing and grooming were present in open-field tests, but this was not statistically significant, discarding nonspecific motor alterations. The alterations found in this study are possibly related to intrinsic obesity and metabolic complications present in the Zucker rat model, where leptin plays an important role in animal mood, more so than sterculic oil consumption.
Meza-Espinoza, Libier,Vivar-Vera, Maria de los Angeles,Garcia-Magana, Maria de Lourdes,Sayago-Ayerdi, Sonia G.,Chacon-Lopez, Alejandra,Becerrea-Verdin, Eduardo M.,Montalvo-Gonzalez, Efigenia 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
The enzymatic activity and partial characterization of proteases from Bromelia karatas fruits were evaluated and compared with Bromelia pinguin proteases. The specific activity increased twofold after partial purification in both proteases. Partially purified proteases from Bromelia karatas showed good specific activity at pH 6.0-8.0 and residual activity of 70-100% for 60 min at $37-60^{\circ}C$, similar to Bromelia pinguin proteases. The $K_m$ value of proteases from Bromelia karatas was higher ($253.32{\mu}M$) than that of Bromelia pinguin proteases ($234.94{\mu}M$). The use of specific protease inhibitors indicated the presence of cysteine and serine proteases. Proteases with molecular weight of 66.2-97 and 21-31 kDa were detected. Bromelia karatas proteases registered 73% hydrolysis using a soy protein concentrate, similar to the enzyme activity of Bromelia pinguin proteases and commercial bromelain. These results demonstrate that Bromelia karatas proteases could be a potential alternative protease in the food industry.
Libier Meza-Espinoza,Marı´a de los A ´ ngeles Vivar-Vera,Marı´a de Lourdes Garcı´a-Magan˜a,Sonia G. Sa´yago-Ayerdi,Alejandra Chaco´n-Lo´pez,Eduardo M. Becerrea-Verdı´n,Efigenia Montalvo-Gonza´lez 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
The enzymatic activity and partial characterization of proteases from Bromelia karatas fruits were evaluated and compared with Bromelia pinguin proteases. The specific activity increased twofold after partial purification in both proteases. Partially purified proteases from Bromelia karatas showed good specific activity at pH 6.0–8.0 and residual activity of 70–100% for 60 min at 37–60 C, similar to Bromelia pinguin proteases. The Km value of proteases from Bromelia karatas was higher (253.32 lM) than that of Bromelia pinguin proteases (234.94 lM). The use of specific protease inhibitors indicated the presence of cysteine and serine proteases. Proteases with molecular weight of 66.2–97 and 21–31 kDa were detected. Bromelia karatas proteases registered 73% hydrolysis using a soy protein concentrate, similar to the enzyme activity of Bromelia pinguin proteases and commercial bromelain. These results demonstrate that Bromelia karatas proteases could be a potential alternative protease in the food industry.
Climate Change Emotions on YouTube: The Case of Before the Flood
Xanat Vargas Meza,Matthew Shapiro,박한우 한국자료분석학회 2018 Journal of the Korean Data Analysis Society Vol.20 No.4
This study examines public comments on the trailer for Before the Flood, a climate change-oriented documentary released in October 2016. Through webometrics, semantic and sentiment analyses, emotions related to climate change were explored. Sentiment analysis of comments on videos examined in previous studies was compared with a sentiment analysis of post-video comments for Before the Flood. Relevant influencers on the comment network were identified, their discourse analyzed in depth, and the results showed an acute politicization of comments, partly due to the electoral campaign ongoing in the United States; while usage of scientific terms decreased. Influential users tended to express polarized views in favor or against the view that climate change is real. However, sentiment regarding climate change remained stable. This study contributes to the intersection of webometrics with environmental psychology as a useful tool to analyze media, particularly if it is targeted to a global audience.
J.A. Morán Meza,J. Polesel-Maris,C. Lubin,F. Thoyer,A. Makky,A. Ouerghi,J. Cousty 한국물리학회 2015 Current Applied Physics Vol.15 No.9
Sharp Pt/Ir tips have been reproducibly etched by an electrochemical process using an inverse geometry of an electrochemical cell and a dedicated electronic device which allows us to control the applied voltages waveform and the intensity of the etching current. Conductive tips with a radius smaller than 10 nm were routinely produced as shown by field emission measurements through Fowler-Nordheim plots. These etched tips were then fixed on a quartz tuning fork force sensor working in a qPlus configuration to check their performances for both scanning tunneling microscopy (STM) and atomic force microscopy (AFM) imaging. Their sharpness and conductivity are evidenced by the resolution achieved in STM and AFM images obtained of epitaxial graphene on 6H-SiC(0001) surface. The structure of an epitaxial graphene layer thermally grown on the 6H-SiC(0001) (6 √3 × 6 √3 ) R30˚ reconstructed surface, was successfully imaged at room temperature with STM, dynamic STM and by frequency modulated AFM.
Rosa Isela Guzman-Geronimo,Tania Margarita Alarcon-Zavaleta,Rosa Marıa Oliart-Ros,Jose Enrique Meza-Alvarado,Socorro Herrera-Meza,Jose Luis Chavez-Servia 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.2
The effect of blue maize extract in factors related to metabolic syndrome (MS) in Wistar rats was investigated. Total polyphenols, monomeric anthocyanins, and antioxidant activity were analyzed in blue maize. MS was induced in Wistar rats fed with high-sucrose (HS) diet for 12 weeks. During a period of 4 weeks, blue maize extract was administrated to HS groups fed with high-sucrose and high-cholesterol–high-sucrose (HS+C) diets. In the blue maize extract administered by orogastric cannulation, the levels of total polyphenols and anthocyanins were 9.97 and 2.92 mg/kg of weight, respectively. HS diet administered during a period of 12 weeks increased significantly systolic blood pressure, serum triglycerides, and decreased high-density lipoprotein cholesterol (HDL-C), alterations related to the MS. Abdominal adipose tissue was only increased in the HS + C group. Blue maize extract administration enhanced HDL-C and decreased systolic blood pressure, serum triglycerides, total cholesterol, and epididymal adipose tissue weight. The blue maize may represent a promising nutraceutical option for the treatment of MS.